CK739983

Overview
NameCK739983
Unique NameCK739983
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length508
Libraries
Library NameType
Ridge pineapple sweet orange entire seedlingcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig13340 contig Csv1_Contig13340:11..518. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CK739983 vs. ExPASy Swiss-Prot
Match: RL27_PEA (60S ribosomal protein L27 OS=Pisum sativum GN=RPL27 PE=2 SV=1)

HSP 1 Score: 181.03 bits (458), Expect = 4.346e-49
Identity = 92/111 (82.88%), Postives = 96/111 (86.49%), Query Frame = 2 
Query:  137 IVKSFDDRTRERPNGHCLVAGIKK*PGKVIHKDSAKKTAK*SRVKAFVKLVSYQHLMPTRYTLDVDLKVVVTADSLQSKDKKMIACKETKKRLEERFKTSKNRWFFTKLRF 469
            IVK+FDD TRE+P GHCLVAGIKK P KVI KDSAKKTAK SRVKAFVKLV+YQHLMPTRYTLDVDLK  V  D LQSKDKK+ A KETKK LEERFKT KNRWFFTKLRF
Sbjct:   25 IVKTFDDGTREKPYGHCLVAGIKKFPSKVIKKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKDAVVPDVLQSKDKKVTALKETKKSLEERFKTGKNRWFFTKLRF 135

HSP 2 Score: 33.4982 bits (75), Expect = 4.346e-49
Identity = 15/18 (83.33%), Postives = 16/18 (88.89%), Query Frame = 3 
Query:   84 FLKPNKTVILL*GRYAGR 137
            FLKPNK VILL GRYAG+
Sbjct:    4 FLKPNKAVILLQGRYAGK 21
[Close]
BLAST of CK739983 vs. ExPASy Swiss-Prot
Match: RL273_ARATH (60S ribosomal protein L27-3 OS=Arabidopsis thaliana GN=RPL27C PE=2 SV=2)

HSP 1 Score: 177.948 bits (450), Expect = 3.015e-47
Identity = 87/111 (78.38%), Postives = 95/111 (85.59%), Query Frame = 2 
Query:  137 IVKSFDDRTRERPNGHCLVAGIKK*PGKVIHKDSAKKTAK*SRVKAFVKLVSYQHLMPTRYTLDVDLKVVVTADSLQSKDKKMIACKETKKRLEERFKTSKNRWFFTKLRF 469
            I+KSFDD  R+RP GHCLVAG+KK P KVI KDSAKKTAK SRVK F+KLV+YQHLMPTRYTLDVDLK V T D+LQSKDKK+ A KE K +LEERFKT KNRWFFTKLRF
Sbjct:   25 IIKSFDDGNRDRPYGHCLVAGLKKYPSKVIRKDSAKKTAKKSRVKCFIKLVNYQHLMPTRYTLDVDLKEVATLDALQSKDKKVAALKEAKAKLEERFKTGKNRWFFTKLRF 135

HSP 2 Score: 30.4166 bits (67), Expect = 3.015e-47
Identity = 14/18 (77.78%), Postives = 15/18 (83.33%), Query Frame = 3 
Query:   84 FLKPNKTVILL*GRYAGR 137
            FLK NK VILL GRYAG+
Sbjct:    4 FLKQNKAVILLQGRYAGK 21
[Close]
BLAST of CK739983 vs. ExPASy Swiss-Prot
Match: RL272_ARATH (60S ribosomal protein L27-2 OS=Arabidopsis thaliana GN=RPL27B PE=2 SV=2)

HSP 1 Score: 172.555 bits (436), Expect = 1.230e-45
Identity = 85/111 (76.58%), Postives = 94/111 (84.68%), Query Frame = 2 
Query:  137 IVKSFDDRTRERPNGHCLVAGIKK*PGKVIHKDSAKKTAK*SRVKAFVKLVSYQHLMPTRYTLDVDLKVVVTADSLQSKDKKMIACKETKKRLEERFKTSKNRWFFTKLRF 469
            I+KSFDD T +R  GHCLVAG+KK P KVI KDSAKKTAK SRVK F+KLV+YQHLMPTRYTLDVDLK V T D+L+SKDKK+ A KE K +LEERFKT KNRWFFTKLRF
Sbjct:   25 IIKSFDDGTSDRRYGHCLVAGLKKYPSKVIRKDSAKKTAKKSRVKCFIKLVNYQHLMPTRYTLDVDLKEVATLDALKSKDKKVTALKEAKAKLEERFKTGKNRWFFTKLRF 135

HSP 2 Score: 30.4166 bits (67), Expect = 1.230e-45
Identity = 14/18 (77.78%), Postives = 15/18 (83.33%), Query Frame = 3 
Query:   84 FLKPNKTVILL*GRYAGR 137
            FLK NK VILL GRYAG+
Sbjct:    4 FLKQNKAVILLQGRYAGK 21
[Close]
BLAST of CK739983 vs. ExPASy Swiss-Prot
Match: RL271_ARATH (60S ribosomal protein L27-1 OS=Arabidopsis thaliana GN=RPL27A PE=2 SV=1)

HSP 1 Score: 166.007 bits (419), Expect = 1.567e-42
Identity = 81/111 (72.97%), Postives = 93/111 (83.78%), Query Frame = 2 
Query:  137 IVKSFDDRTRERPNGHCLVAGIKK*PGKVIHKDSAKKTAK*SRVKAFVKLVSYQHLMPTRYTLDVDLKVVVTADSLQSKDKKMIACKETKKRLEERFKTSKNRWFFTKLRF 469
            IVKSFDD T E+  GHCLVAG+KK P KVI KDSAKKTAK SRVK F K+++YQH+MPTRYTLD+DLK VV+AD++ SKDKK+ A KE K + EERFKT KNRWFFTKLRF
Sbjct:   25 IVKSFDDGTVEKKYGHCLVAGLKKYPSKVIRKDSAKKTAKKSRVKCFFKVINYQHVMPTRYTLDLDLKNVVSADAISSKDKKVTALKEAKAKFEERFKTGKNRWFFTKLRF 135

HSP 2 Score: 26.5646 bits (57), Expect = 1.567e-42
Identity = 11/17 (64.71%), Postives = 13/17 (76.47%), Query Frame = 3 
Query:   87 LKPNKTVILL*GRYAGR 137
            +KP K VILL GRY G+
Sbjct:    5 MKPGKAVILLQGRYTGK 21
[Close]
BLAST of CK739983 vs. ExPASy Swiss-Prot
Match: RL27_SOLTU (60S ribosomal protein L27 OS=Solanum tuberosum GN=RPL27 PE=2 SV=1)

HSP 1 Score: 151.754 bits (382), Expect = 2.393e-40
Identity = 79/115 (68.70%), Postives = 91/115 (79.13%), Query Frame = 2 
Query:  137 IVKSFDDRTRERPNGHCLVAGIKK*PGKVIHKDSAKKTAK*SRVKAFVKLVSYQHLMPTRYTLDVDL----KVVVTADSLQSKDKKMIACKETKKRLEERFKTSKNRWFFTKLRF 469
            IV++FD+ TR+RP GHCLVAGI + P KVI KDSAKK  K SRVKAF+KLV+Y H+MPTRYTLD DL    K VV AD LQ++DKK+ A + TK RL ERFKT KNRWFFTKLRF
Sbjct:   25 IVRAFDEGTRDRPYGHCLVAGISRYPKKVIRKDSAKKAGKKSRVKAFIKLVNYNHIMPTRYTLDEDLKDVVKDVVNADVLQARDKKVTA-RRTKARLAERFKTGKNRWFFTKLRF 138

HSP 2 Score: 33.4982 bits (75), Expect = 2.393e-40
Identity = 15/18 (83.33%), Postives = 16/18 (88.89%), Query Frame = 3 
Query:   84 FLKPNKTVILL*GRYAGR 137
            FLKPNK VILL G+YAGR
Sbjct:    4 FLKPNKAVILLQGKYAGR 21
[Close]
BLAST of CK739983 vs. ExPASy Swiss-Prot
Match: RL27_PYRST (60S ribosomal protein L27 OS=Pyrobotrys stellata GN=RPL27 PE=2 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 2.164e-31
Identity = 67/111 (60.36%), Postives = 81/111 (72.97%), Query Frame = 2 
Query:  137 IVKSFDDRTRERPNGHCLVAGIKK*PGKVIHKDSAKKTAK*SRVKAFVKLVSYQHLMPTRYTLDVDLKVVVTADSLQSKDKKMIACKETKKRLEERFKTSKNRWFFTKLRF 469
            IV++FDD T  RP GH LV G++K P KV  + S KK AK S +K F+K V+Y HLMPTRYTLDVD K  V A++ ++  KK+ A KE KK LEE+FKT KNRWFFTKLRF
Sbjct:   25 IVRNFDDGTSSRPYGHALVVGLQKEPRKVTKRQSQKKQAKKSTLKTFIKTVNYNHLMPTRYTLDVDFK-GVAAEAQENPTKKVEARKECKKLLEEKFKTGKNRWFFTKLRF 134

HSP 2 Score: 25.0238 bits (53), Expect = 2.164e-31
Identity = 10/18 (55.56%), Postives = 14/18 (77.78%), Query Frame = 3 
Query:   84 FLKPNKTVILL*GRYAGR 137
            FLK  K V++L GR+AG+
Sbjct:    4 FLKSGKVVVVLSGRFAGK 21
[Close]
BLAST of CK739983 vs. ExPASy Swiss-Prot
Match: RL27_HIPCM (60S ribosomal protein L27 OS=Hippocampus comes GN=rpl27 PE=2 SV=2)

HSP 1 Score: 112.464 bits (280), Expect = 2.224e-27
Identity = 59/112 (52.68%), Postives = 74/112 (66.07%), Query Frame = 2 
Query:  137 IVKSFDDRTRERPNGHCLVAGIKK*PGKVIHKDSAKKTAK*SRVKAFVKLVSYQHLMPTRYTLDVDL-KVVVTADSLQSKDKKMIACKETKKRLEERFKTSKNRWFFTKLRF 469
            IVK+ DD T +RP  H LVAGI + P KV      KK AK S++KAFVK+ +Y HLMPTRY++D+ L K VV  D  +    K  A +E K + EER+KT KN+WFF KLRF
Sbjct:   25 IVKNIDDGTADRPYSHALVAGIDRYPRKVTAPMGKKKIAKRSKIKAFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKSKARREAKVKFEERYKTGKNKWFFQKLRF 136

HSP 2 Score: 29.261 bits (64), Expect = 2.224e-27
Identity = 12/18 (66.67%), Postives = 15/18 (83.33%), Query Frame = 3 
Query:   84 FLKPNKTVILL*GRYAGR 137
            F+KP K V++L GRYAGR
Sbjct:    4 FMKPGKVVMVLAGRYAGR 21
[Close]
BLAST of CK739983 vs. ExPASy Swiss-Prot
Match: RL27_DANRE (60S ribosomal protein L27 OS=Danio rerio GN=rpl27 PE=2 SV=3)

HSP 1 Score: 112.464 bits (280), Expect = 2.224e-27
Identity = 59/112 (52.68%), Postives = 74/112 (66.07%), Query Frame = 2 
Query:  137 IVKSFDDRTRERPNGHCLVAGIKK*PGKVIHKDSAKKTAK*SRVKAFVKLVSYQHLMPTRYTLDVDL-KVVVTADSLQSKDKKMIACKETKKRLEERFKTSKNRWFFTKLRF 469
            IVK+ DD T +RP  H LVAGI + P KV      KK AK S++KAFVK+ +Y HLMPTRY++D+ L K VV  D  +    K  A +E K + EER+KT KN+WFF KLRF
Sbjct:   25 IVKNIDDGTADRPYSHALVAGIDRYPRKVTATMGKKKIAKRSKIKAFVKVFNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF 136

HSP 2 Score: 29.261 bits (64), Expect = 2.224e-27
Identity = 12/18 (66.67%), Postives = 15/18 (83.33%), Query Frame = 3 
Query:   84 FLKPNKTVILL*GRYAGR 137
            F+KP K V++L GRYAGR
Sbjct:    4 FMKPGKVVMVLAGRYAGR 21
[Close]
BLAST of CK739983 vs. ExPASy Swiss-Prot
Match: RL27_ICTPU (60S ribosomal protein L27 OS=Ictalurus punctatus GN=rpl27 PE=2 SV=3)

HSP 1 Score: 111.309 bits (277), Expect = 4.907e-27
Identity = 57/112 (50.89%), Postives = 74/112 (66.07%), Query Frame = 2 
Query:  137 IVKSFDDRTRERPNGHCLVAGIKK*PGKVIHKDSAKKTAK*SRVKAFVKLVSYQHLMPTRYTLDVDL-KVVVTADSLQSKDKKMIACKETKKRLEERFKTSKNRWFFTKLRF 469
            IVK+ DD T +RP  H LV+GI + P KV      KK AK S++KAFVK+ +Y HLMPTRY++D+ L K +V  D  +    K  A +E K + EER+KT KN+WFF KLRF
Sbjct:   25 IVKNIDDGTADRPYSHALVSGIDRYPRKVTATMGKKKVAKRSKIKAFVKVFNYNHLMPTRYSVDIPLDKTIVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF 136

HSP 2 Score: 29.261 bits (64), Expect = 4.907e-27
Identity = 12/18 (66.67%), Postives = 15/18 (83.33%), Query Frame = 3 
Query:   84 FLKPNKTVILL*GRYAGR 137
            F+KP K V++L GRYAGR
Sbjct:    4 FMKPGKVVMVLAGRYAGR 21
[Close]
BLAST of CK739983 vs. ExPASy Swiss-Prot
Match: RL27_RAT (60S ribosomal protein L27 OS=Rattus norvegicus GN=Rpl27 PE=2 SV=2)

HSP 1 Score: 111.309 bits (277), Expect = 8.315e-27
Identity = 58/112 (51.79%), Postives = 74/112 (66.07%), Query Frame = 2 
Query:  137 IVKSFDDRTRERPNGHCLVAGIKK*PGKVIHKDSAKKTAK*SRVKAFVKLVSYQHLMPTRYTLDVDL-KVVVTADSLQSKDKKMIACKETKKRLEERFKTSKNRWFFTKLRF 469
            IVK+ DD T +RP  H LVAGI + P KV      KK AK S++K+FVK+ +Y HLMPTRY++D+ L K VV  D  +    K  A +E K + EER+KT KN+WFF KLRF
Sbjct:   25 IVKNIDDGTSDRPYSHALVAGIDRYPRKVTAAMGKKKIAKRSKIKSFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF 136

HSP 2 Score: 28.4906 bits (62), Expect = 8.315e-27
Identity = 11/18 (61.11%), Postives = 15/18 (83.33%), Query Frame = 3 
Query:   84 FLKPNKTVILL*GRYAGR 137
            F+KP K V++L GRY+GR
Sbjct:    4 FMKPGKVVLVLAGRYSGR 21
The following BLAST results are available for this feature:
BLAST of CK739983 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 25
Match NameE-valueIdentityDescription
RL27_PEA4.346e-4982.8860S ribosomal protein L27 OS=Pisum sativum GN=RPL2... [more]
RL273_ARATH3.015e-4778.3860S ribosomal protein L27-3 OS=Arabidopsis thalian... [more]
RL272_ARATH1.230e-4576.5860S ribosomal protein L27-2 OS=Arabidopsis thalian... [more]
RL271_ARATH1.567e-4272.9760S ribosomal protein L27-1 OS=Arabidopsis thalian... [more]
RL27_SOLTU2.393e-4068.7060S ribosomal protein L27 OS=Solanum tuberosum GN=... [more]
RL27_PYRST2.164e-3160.3660S ribosomal protein L27 OS=Pyrobotrys stellata G... [more]
RL27_HIPCM2.224e-2752.6860S ribosomal protein L27 OS=Hippocampus comes GN=... [more]
RL27_DANRE2.224e-2752.6860S ribosomal protein L27 OS=Danio rerio GN=rpl27 ... [more]
RL27_ICTPU4.907e-2750.8960S ribosomal protein L27 OS=Ictalurus punctatus G... [more]
RL27_RAT8.315e-2751.7960S ribosomal protein L27 OS=Rattus norvegicus GN=... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_6477 Ridge pineapple sweet orange entire seedling Citrus sinensis cDNA clone RSE64A04 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK739983 ID=CK739983; Name=CK739983; organism=Citrus sinensis; type=EST; length=508bp
CACGTTGCCAGCCTCTGCACAAACACACAAAAACCCAGAGAGAGTCGCGG
GCGCGCGAGGAGTGAGAGTTGAAATGGTGACCGTTTCTGAAACCAAATAA
GACCGTGATCCTTCTTTAGGGCCGTTACGCCGGCCGATTGTGAAGTCGTT
CGACGACAGGACCCGCGAGAGGCCGAACGGGCACTGTCTGGTAGCGGGGA
TAAAGAAGTAACCGGGCAAAGTGATCCACAAGGACTCGGCGAAGAAGACA
GCGAAGTAGTCACGCGTCAAGGCCTTCGTCAAGTTGGTCAGCTACCAGCA
CCTGATGCCCACGCGCTACACTCTCGACGTCGATTTGAAGGTAGTCGTGA
CGGCTGATTCCCTCCAGAGCAAGGACAAGAAGATGATAGCTTGCAAGGAG
ACCAAGAAACGCCTCGAGGAGAGGTTTAAGACTAGCAAGAATCGTTGGTT
CTTTACTAAGCTCAGGTTTTAGATTTTTAACCAAAGGAAGAAAACGACTT
GTCGTTTT
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