CK932932 | Citrus Genome Database

Overview


Name	CK932932
Unique Name	CK932932
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	699

Libraries


Library Name	Type
Developing fruit juice sac at 38 DAFB	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
CK932932_ssr83	microsatellite	CK932932_ssr83:83..109.	BLAST: Citrus ESTs to Prunus persica proteins V1
CK932932_ssr105	microsatellite	CK932932_ssr105:105..116.	BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig9730	contig	Csv1_Contig9730:1..693.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CK932932 vs. ExPASy Swiss-Prot
Match: PIP27_ARATH (Aquaporin PIP2-7 OS=Arabidopsis thaliana GN=PIP2-7 PE=1 SV=2)

HSP 1 Score: 294.278 bits (752), Expect = 4.742e-85
Identity = 140/161 (86.96%), Postives = 150/161 (93.17%), Query Frame = 2

Query:  158 HHGKDYVDPPPAPLIDMAELKLWSFYRALIAEFVATLLFLYVSVATVIGHKKQSDACGGVGLLGIAWAFGGMIFVLVYCTAGISGGHINPAVAFGLFLARKVSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTF 640
            HHGKDYVDPPPAPL+DM ELK WSFYRALIAEF+ATLLFLYV+VATVIGHKKQ+  C GVGLLGIAWAFGGMIFVLVYCTAGISGGHINPAV FGLFLARKVSL+RA+ YM+AQCLGAICGVG VKAFMK  YN+LGGGANTVA GY+KG+ALGAEIIGTF
Sbjct:   12 HHGKDYVDPPPAPLLDMGELKSWSFYRALIAEFIATLLFLYVTVATVIGHKKQTGPCDGVGLLGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLVRALGYMIAQCLGAICGVGFVKAFMKTPYNTLGGGANTVADGYSKGTALGAEIIGTF 172

HSP 2 Score: 42.743 bits (99), Expect = 4.742e-85
Identity = 20/20 (100.00%), Postives = 20/20 (100.00%), Query Frame = 3

Query:  639 FVLVYTVFSATDPKRSARDS 698
            FVLVYTVFSATDPKRSARDS
Sbjct:  172 FVLVYTVFSATDPKRSARDS 191

[Close]

BLAST of CK932932 vs. ExPASy Swiss-Prot
Match: PIP28_ARATH (Probable aquaporin PIP2-8 OS=Arabidopsis thaliana GN=PIP2-8 PE=1 SV=1)

HSP 1 Score: 289.656 bits (740), Expect = 1.151e-83
Identity = 141/160 (88.12%), Postives = 148/160 (92.50%), Query Frame = 2

Query:  161 HGKDYVDPPPAPLIDMAELKLWSFYRALIAEFVATLLFLYVSVATVIGHKKQSDACGGVGLLGIAWAFGGMIFVLVYCTAGISGGHINPAVAFGLFLARKVSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTF 640
            HGKDYVDPPPAPL+DMAELKLWSFYRA+IAEF+ATLLFLYV+VATVIGHK Q+  CGGVGLLGIAWAFGGMIFVLVYCTAGISGGHINPAV FGLFLARKVSL RAVAYMVAQCLGAICGVGLVKAFM   Y  LGGGANTVA GY+ G+ALGAEIIGTF
Sbjct:   11 HGKDYVDPPPAPLLDMAELKLWSFYRAIIAEFIATLLFLYVTVATVIGHKNQTGPCGGVGLLGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLPRAVAYMVAQCLGAICGVGLVKAFMMTPYKRLGGGANTVADGYSTGTALGAEIIGTF 170

HSP 2 Score: 42.743 bits (99), Expect = 1.151e-83
Identity = 20/20 (100.00%), Postives = 20/20 (100.00%), Query Frame = 3

Query:  639 FVLVYTVFSATDPKRSARDS 698
            FVLVYTVFSATDPKRSARDS
Sbjct:  170 FVLVYTVFSATDPKRSARDS 189

[Close]

BLAST of CK932932 vs. ExPASy Swiss-Prot
Match: PIP1_ATRCA (Aquaporin PIP-type OS=Atriplex canescens PE=2 SV=1)

HSP 1 Score: 280.796 bits (717), Expect = 3.051e-81
Identity = 131/162 (80.86%), Postives = 145/162 (89.51%), Query Frame = 2

Query:  155 HHHGKDYVDPPPAPLIDMAELKLWSFYRALIAEFVATLLFLYVSVATVIGHKKQSDACGGVGLLGIAWAFGGMIFVLVYCTAGISGGHINPAVAFGLFLARKVSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTF 640
            H HGKDYVDPPPAP  DM ELKLWSF+RA IAEF+ATLLFLY++VATVIG+KK++D C  VGLLGIAW+FGGMIFVLVYCTAGISGGHINPAV FGLFLARKVSL+RA+ YM+AQC GAICGVGLVKAFMK  YN  GGGAN+VA GYNKG+A GAE+IGTF
Sbjct:   13 HQHGKDYVDPPPAPFFDMGELKLWSFWRAAIAEFIATLLFLYITVATVIGYKKETDPCASVGLLGIAWSFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLLRALVYMIAQCAGAICGVGLVKAFMKGPYNQFGGGANSVALGYNKGTAFGAELIGTF 174

HSP 2 Score: 43.5134 bits (101), Expect = 3.051e-81
Identity = 21/24 (87.50%), Postives = 22/24 (91.67%), Query Frame = 3

Query:  627 LLVPFVLVYTVFSATDPKRSARDS 698
            L+  FVLVYTVFSATDPKRSARDS
Sbjct:  170 LIGTFVLVYTVFSATDPKRSARDS 193

[Close]

BLAST of CK932932 vs. ExPASy Swiss-Prot
Match: PIP26_ORYSJ (Probable aquaporin PIP2-6 OS=Oryza sativa subsp. japonica GN=PIP2-6 PE=2 SV=2)

HSP 1 Score: 265.388 bits (677), Expect = 4.748e-76
Identity = 129/164 (78.66%), Postives = 143/164 (87.20%), Query Frame = 2

Query:  155 HHHGKDYVDPPPAPLIDMAELKLWSFYRALIAEFVATLLFLYVSVATVIGHKKQS--DACGGVGLLGIAWAFGGMIFVLVYCTAGISGGHINPAVAFGLFLARKVSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTF 640
            H   KDY DPPPAPL D+ EL+LWSFYRALIAEF+ATLLFLY++VATVIG+K QS  D CGGVG LGIAWAFGGMIF+LVYCTAGISGGHINPAV FGL LARKVS+IRAV Y+VAQCLG I GVG+VK  MKH+YN+ GGGAN VASGY+ G+ALGAEIIGTF
Sbjct:   11 HVRPKDYTDPPPAPLFDVGELRLWSFYRALIAEFIATLLFLYITVATVIGYKVQSSADQCGGVGTLGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLLLARKVSVIRAVMYIVAQCLGGIVGVGIVKGIMKHQYNANGGGANMVASGYSTGTALGAEIIGTF 174

HSP 2 Score: 41.5874 bits (96), Expect = 4.748e-76
Identity = 19/20 (95.00%), Postives = 20/20 (100.00%), Query Frame = 3

Query:  639 FVLVYTVFSATDPKRSARDS 698
            FVLVYTVFSATDPKR+ARDS
Sbjct:  174 FVLVYTVFSATDPKRNARDS 193

[Close]

BLAST of CK932932 vs. ExPASy Swiss-Prot
Match: PIP21_ORYSJ (Probable aquaporin PIP2-1 OS=Oryza sativa subsp. japonica GN=PIP2-1 PE=2 SV=1)

HSP 1 Score: 259.225 bits (661), Expect = 3.327e-74
Identity = 126/166 (75.90%), Postives = 142/166 (85.54%), Query Frame = 2

Query:  167 KDYVDPPPAPLIDMAELKLWSFYRALIAEFVATLLFLYVSVATVIGHKKQSDA--------CGGVGLLGIAWAFGGMIFVLVYCTAGISGGHINPAVAFGLFLARKVSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTF 640
            KDY DPPPAPLID AEL  WS YRA+IAEF+ATLLFLY++VATVIG+K Q+DA        CGGVG+LGIAWAFGGMIF+LVYCTAGISGGHINPAV FGLFLARKVSL+RA+ Y+VAQCLGAICGVGLVKAF    +N  GGGANT+A+GY+KG+ L AEIIGTF
Sbjct:   19 KDYTDPPPAPLIDAAELGSWSLYRAVIAEFIATLLFLYITVATVIGYKHQTDASASGADAACGGVGVLGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLVRAILYIVAQCLGAICGVGLVKAFQSAYFNRYGGGANTLAAGYSKGTGLAAEIIGTF 184

HSP 2 Score: 41.5874 bits (96), Expect = 3.327e-74
Identity = 19/20 (95.00%), Postives = 20/20 (100.00%), Query Frame = 3

Query:  639 FVLVYTVFSATDPKRSARDS 698
            FVLVYTVFSATDPKR+ARDS
Sbjct:  184 FVLVYTVFSATDPKRNARDS 203

[Close]

BLAST of CK932932 vs. ExPASy Swiss-Prot
Match: PIP22_MAIZE (Aquaporin PIP2-2 OS=Zea mays GN=PIP2-2 PE=2 SV=1)

HSP 1 Score: 257.684 bits (657), Expect = 9.625e-74
Identity = 125/168 (74.40%), Postives = 143/168 (85.12%), Query Frame = 2

Query:  167 KDYVDPPPAPLIDMAELKLWSFYRALIAEFVATLLFLYVSVATVIGHKKQSDA----------CGGVGLLGIAWAFGGMIFVLVYCTAGISGGHINPAVAFGLFLARKVSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTF 640
            KDY DPPPAPL+D AEL  WS YRA+IAEF+ATLLFLYV+VATVIG+K Q+DA          CGGVG+LGIAWAFGGMIFVLVYCTAGISGGHINPAV FGLFLARKVSL+RA+ YMVAQCLGA+CGVGLVKAF    ++  GGGAN++ASGY++G+ LGAEI+GTF
Sbjct:   19 KDYTDPPPAPLVDAAELGSWSLYRAVIAEFIATLLFLYVTVATVIGYKHQTDASASGAGADAACGGVGVLGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLVRALLYMVAQCLGAVCGVGLVKAFQSAYFDRYGGGANSLASGYSRGAGLGAEIVGTF 186

HSP 2 Score: 41.5874 bits (96), Expect = 9.625e-74
Identity = 19/20 (95.00%), Postives = 20/20 (100.00%), Query Frame = 3

Query:  639 FVLVYTVFSATDPKRSARDS 698
            FVLVYTVFSATDPKR+ARDS
Sbjct:  186 FVLVYTVFSATDPKRNARDS 205

[Close]

BLAST of CK932932 vs. ExPASy Swiss-Prot
Match: PIP21_MAIZE (Aquaporin PIP2-1 OS=Zea mays GN=PIP2-1 PE=1 SV=2)

HSP 1 Score: 257.684 bits (657), Expect = 9.625e-74
Identity = 126/166 (75.90%), Postives = 143/166 (86.14%), Query Frame = 2

Query:  167 KDYVDPPPAPLIDMAELKLWSFYRALIAEFVATLLFLYVSVATVIGHKKQSDA--------CGGVGLLGIAWAFGGMIFVLVYCTAGISGGHINPAVAFGLFLARKVSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTF 640
            KDY DPPPAPLID AEL  WS YRA+IAEF+ATLLFLY++VATVIG+K Q+DA        CGGVG+LGIAWAFGGMIFVLVYCTAGISGGHINPAV FGLFLARKVSL+RA+ Y+VAQCLGAICGVGLVKAF    ++  GGGAN++ASGY++G+ LGAEIIGTF
Sbjct:   19 KDYTDPPPAPLIDAAELGSWSLYRAVIAEFIATLLFLYITVATVIGYKHQTDASASGADAACGGVGVLGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLVRALLYIVAQCLGAICGVGLVKAFQSAYFDRYGGGANSLASGYSRGTGLGAEIIGTF 184

HSP 2 Score: 41.5874 bits (96), Expect = 9.625e-74
Identity = 19/20 (95.00%), Postives = 20/20 (100.00%), Query Frame = 3

Query:  639 FVLVYTVFSATDPKRSARDS 698
            FVLVYTVFSATDPKR+ARDS
Sbjct:  184 FVLVYTVFSATDPKRNARDS 203

[Close]

BLAST of CK932932 vs. ExPASy Swiss-Prot
Match: PIP22_ORYSJ (Probable aquaporin PIP2-2 OS=Oryza sativa subsp. japonica GN=PIP2-2 PE=2 SV=1)

HSP 1 Score: 253.832 bits (647), Expect = 1.370e-72
Identity = 124/166 (74.70%), Postives = 138/166 (83.13%), Query Frame = 2

Query:  167 KDYVDPPPAPLIDMAELKLWSFYRALIAEFVATLLFLYVSVATVIGHKKQSDA--------CGGVGLLGIAWAFGGMIFVLVYCTAGISGGHINPAVAFGLFLARKVSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTF 640
            KDY DPPPAPLID+ EL  WS YRA+IAEF+ATLLFLY++VATVIG+K QSDA        C GVG+LGIAWAFGGMIF+LVYCTAGISGGHINPAV FGLFLARKVSLIRAV Y++AQCLGAICGVGLVK F    Y   GGGAN ++ GY+KG+ LGAEIIGTF
Sbjct:   18 KDYTDPPPAPLIDVEELTKWSLYRAVIAEFIATLLFLYITVATVIGYKHQSDATVNTTDAACSGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLIRAVLYIIAQCLGAICGVGLVKGFQSSYYARYGGGANELSDGYSKGTGLGAEIIGTF 183

HSP 2 Score: 41.5874 bits (96), Expect = 1.370e-72
Identity = 19/20 (95.00%), Postives = 20/20 (100.00%), Query Frame = 3

Query:  639 FVLVYTVFSATDPKRSARDS 698
            FVLVYTVFSATDPKR+ARDS
Sbjct:  183 FVLVYTVFSATDPKRNARDS 202

[Close]

BLAST of CK932932 vs. ExPASy Swiss-Prot
Match: PIP25_MAIZE (Aquaporin PIP2-5 OS=Zea mays GN=PIP2-5 PE=1 SV=1)

HSP 1 Score: 253.832 bits (647), Expect = 1.371e-72
Identity = 123/169 (72.78%), Postives = 140/169 (82.84%), Query Frame = 2

Query:  158 HHGKDYVDPPPAPLIDMAELKLWSFYRALIAEFVATLLFLYVSVATVIGHKKQSDA--------CGGVGLLGIAWAFGGMIFVLVYCTAGISGGHINPAVAFGLFLARKVSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTF 640
            H GKDY DPPPAPL+D  EL  WS YRA+IAEFVATLLFLY++VATVIG+K Q+DA        CGGVG+LGIAWAFGGMIF+LVYCTAG+SGGHINPAV FGLFLARKVSL+RA+ Y+VAQCLGAICGVGLVK F    Y   GGGAN +++GY+KG+ L AEIIGTF
Sbjct:   11 HEGKDYSDPPPAPLVDAEELTKWSLYRAVIAEFVATLLFLYITVATVIGYKHQTDAAASGPDAACGGVGVLGIAWAFGGMIFILVYCTAGVSGGHINPAVTFGLFLARKVSLVRALLYIVAQCLGAICGVGLVKGFQSAFYVRYGGGANELSAGYSKGTGLAAEIIGTF 179

HSP 2 Score: 41.5874 bits (96), Expect = 1.371e-72
Identity = 19/20 (95.00%), Postives = 20/20 (100.00%), Query Frame = 3

Query:  639 FVLVYTVFSATDPKRSARDS 698
            FVLVYTVFSATDPKR+ARDS
Sbjct:  179 FVLVYTVFSATDPKRNARDS 198

[Close]

BLAST of CK932932 vs. ExPASy Swiss-Prot
Match: PIP27_MAIZE (Aquaporin PIP2-7 OS=Zea mays GN=PIP2-7 PE=2 SV=1)

HSP 1 Score: 251.521 bits (641), Expect = 6.743e-72
Identity = 121/168 (72.02%), Postives = 135/168 (80.36%), Query Frame = 2

Query:  158 HHGKDYVDPPPAPLIDMAELKLWSFYRALIAEFVATLLFLYVSVATVIGHKKQSDA-------CGGVGLLGIAWAFGGMIFVLVYCTAGISGGHINPAVAFGLFLARKVSLIRAVAYMVAQCLGAICGVGLVKAFMKHEYNSLGGGANTVASGYNKGSALGAEIIGTF 640
            +  KDY DPPPAPLID  EL  WS YRA IAEF+ATLLFLY++V T+IG+K+QSD        C GVG+LGIAWAFGGMIF+LVYCTAGISGGHINPAV FGLFL RKVSL+RA+ YM+AQC GAICG GL K F K  YN  GGG NTV+ GYNKG+ALGAEIIGTF
Sbjct:   14 YSAKDYHDPPPAPLIDPDELTKWSLYRAAIAEFIATLLFLYITVLTIIGYKRQSDTKIPGNTECDGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLGRKVSLVRALLYMIAQCAGAICGAGLAKGFQKSFYNRYGGGVNTVSDGYNKGTALGAEIIGTF 181

HSP 2 Score: 41.5874 bits (96), Expect = 6.743e-72
Identity = 19/20 (95.00%), Postives = 20/20 (100.00%), Query Frame = 3

Query:  639 FVLVYTVFSATDPKRSARDS 698
            FVLVYTVFSATDPKR+ARDS
Sbjct:  181 FVLVYTVFSATDPKRNARDS 200

The following BLAST results are available for this feature:

BLAST of CK932932 vs. ExPASy Swiss-Prot

BLAST of CK932932 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 124


Match Name	E-value	Identity	Description
PIP27_ARATH	4.742e-85	86.96	Aquaporin PIP2-7 OS=Arabidopsis thaliana GN=PIP2-7...	[more]
PIP28_ARATH	1.151e-83	88.13	Probable aquaporin PIP2-8 OS=Arabidopsis thaliana ...	[more]
PIP1_ATRCA	3.051e-81	80.86	Aquaporin PIP-type OS=Atriplex canescens PE=2 SV=1	[more]
PIP26_ORYSJ	4.748e-76	78.66	Probable aquaporin PIP2-6 OS=Oryza sativa subsp. j...	[more]
PIP21_ORYSJ	3.327e-74	75.90	Probable aquaporin PIP2-1 OS=Oryza sativa subsp. j...	[more]
PIP22_MAIZE	9.625e-74	74.40	Aquaporin PIP2-2 OS=Zea mays GN=PIP2-2 PE=2 SV=1	[more]
PIP21_MAIZE	9.625e-74	75.90	Aquaporin PIP2-1 OS=Zea mays GN=PIP2-1 PE=1 SV=2	[more]
PIP22_ORYSJ	1.370e-72	74.70	Probable aquaporin PIP2-2 OS=Oryza sativa subsp. j...	[more]
PIP25_MAIZE	1.371e-72	72.78	Aquaporin PIP2-5 OS=Zea mays GN=PIP2-5 PE=1 SV=1	[more]
PIP27_MAIZE	6.743e-72	72.02	Aquaporin PIP2-7 OS=Zea mays GN=PIP2-7 PE=2 SV=1	[more]

Pages

Properties


Property Name	Value
Genbank description	CGF1004349_F09 Developing fruit juice sac at 38 DAFB Citrus sinensis cDNA clone JSJune0004_IIIF_F09 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK932932 ID=CK932932; Name=CK932932; organism=Citrus sinensis; type=EST; length=699bp
GGGGACAGACGCTACCTTTTCTCGAGACCAGAGAGGGTGTGTGCTTTTCT
TTGATTGTTCCAAAGAGAAATCGAAATCTTGTGAAGAAGAAGAAGAAGAA
GAAGAAGAAAGAAAGATGTCAAAGGAGGTGAATGAAGAAGGACAAACTCA
CCGCCATCACCATGGCAAGGACTACGTGGACCCACCACCAGCACCACTCA
TTGACATGGCTGAGCTCAAGCTCTGGTCCTTTTACAGAGCTCTCATAGCC
GAGTTCGTGGCCACTCTCCTCTTCCTCTACGTCAGTGTTGCCACTGTCAT
TGGCCACAAGAAACAAAGTGATGCCTGTGGTGGTGTTGGCCTTTTGGGTA
TTGCATGGGCCTTTGGTGGCATGATCTTCGTCCTTGTTTACTGCACCGCT
GGCATTTCTGGTGGTCACATTAACCCTGCTGTGGCGTTTGGGCTGTTCTT
GGCAAGGAAGGTATCATTGATCAGGGCTGTGGCTTACATGGTGGCTCAGT
GCTTGGGTGCCATTTGCGGTGTTGGTTTGGTCAAGGCCTTCATGAAGCAT
GAGTACAACTCACTTGGTGGTGGTGCTAACACTGTGGCTTCTGGCTACAA
CAAAGGATCAGCCTTGGGTGCTGAGATTATTGGTACCTTTCGTGCTTGTT
TACACAGTCTTCTCGGCCACAGACCCCAAGAGAAGCGCTCGTGACTCTC