CK935606 | Citrus Genome Database

Overview


Name	CK935606
Unique Name	CK935606
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	919

Libraries


Library Name	Type
Developing fruit 24 DAFB	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CK935606 vs. ExPASy Swiss-Prot
Match: PSB5B_ARATH (Proteasome subunit beta type-5-B OS=Arabidopsis thaliana GN=PBE2 PE=1 SV=1)

HSP 1 Score: 252.292 bits (643), Expect = 3.354e-97
Identity = 131/164 (79.88%), Postives = 136/164 (82.93%), Query Frame = 1

Query:   64 MKLDTSGLESTSPF--FGSGDELCPEXXXXXXXXXXXXTDFDEFQKDTKATLKHAKGTTTLAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRLHELANKRRISVTGASKLLANILYSYRGMGLSVGTMIAGWDET 549
            MKLDTSGLE+T P   FGS  E+               TDFD FQK     +K AKGTTTLAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRLHELANKRRISV+GASKLLAN+LYSYRGMGLSVGTMIAGWDET
Sbjct:    1 MKLDTSGLETTMPVIGFGSNSEMLDGFSSAPSFDLPRTTDFDGFQKKAVEMVKPAKGTTTLAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRLHELANKRRISVSGASKLLANMLYSYRGMGLSVGTMIAGWDET 164

HSP 2 Score: 102.064 bits (253), Expect = 3.354e-97
Identity = 48/62 (77.42%), Postives = 50/62 (80.65%), Query Frame = 3

Query:  636 GPGLYYVDSEGGRLKGTRFSVGSGSPYAYGVLDSGYKFDMSIXXXXXXXXXXIYHATFRDAA 821
            GPGLYYVD+EGGRLKG RFSVGSGSPYAYGVLDSGYKFDMS+          IYHATFRD A
Sbjct:  165 GPGLYYVDNEGGRLKGDRFSVGSGSPYAYGVLDSGYKFDMSVEEASELARRSIYHATFRDGA 226

HSP 3 Score: 44.669 bits (104), Expect = 3.354e-97
Identity = 17/18 (94.44%), Postives = 17/18 (94.44%), Query Frame = 1

Query:  865 WKKLSGDDVGELHYHYYP 918
            W KLSGDDVGELHYHYYP
Sbjct:  241 WTKLSGDDVGELHYHYYP 258

[Close]

BLAST of CK935606 vs. ExPASy Swiss-Prot
Match: PSB5A_ARATH (Proteasome subunit beta type-5-A OS=Arabidopsis thaliana GN=PBE1 PE=1 SV=1)

HSP 1 Score: 251.906 bits (642), Expect = 9.676e-97
Identity = 129/164 (78.66%), Postives = 136/164 (82.93%), Query Frame = 1

Query:   64 MKLDTSGLESTSPF--FGSGDELCPEXXXXXXXXXXXXTDFDEFQKDTKATLKHAKGTTTLAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRLHELANKRRISVTGASKLLANILYSYRGMGLSVGTMIAGWDET 549
            MKLDTSG E++ P   FGS  ++  E             +FD FQK  K  LKHAKGTTTLAFIFK GVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRLHELANKRRISV+GASKLLAN+LYSYRGMGLSVGTMIAGWDET
Sbjct:    1 MKLDTSGFETSMPMIGFGSSSDMLDELSSVPSFDLPRTKEFDGFQKKAKDMLKHAKGTTTLAFIFKGGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRLHELANKRRISVSGASKLLANMLYSYRGMGLSVGTMIAGWDET 164

HSP 2 Score: 100.908 bits (250), Expect = 9.676e-97
Identity = 47/62 (75.81%), Postives = 50/62 (80.65%), Query Frame = 3

Query:  636 GPGLYYVDSEGGRLKGTRFSVGSGSPYAYGVLDSGYKFDMSIXXXXXXXXXXIYHATFRDAA 821
            GPGLYYVD+EGGRLKG RFSVGSGSPYAYGVLDSGYK+DMS+          IYHATFRD A
Sbjct:  165 GPGLYYVDNEGGRLKGDRFSVGSGSPYAYGVLDSGYKYDMSVEEASELARRSIYHATFRDGA 226

HSP 3 Score: 44.669 bits (104), Expect = 9.676e-97
Identity = 17/18 (94.44%), Postives = 17/18 (94.44%), Query Frame = 1

Query:  865 WKKLSGDDVGELHYHYYP 918
            W KLSGDDVGELHYHYYP
Sbjct:  241 WTKLSGDDVGELHYHYYP 258

[Close]

BLAST of CK935606 vs. ExPASy Swiss-Prot
Match: PSB5_SPIOL (Proteasome subunit beta type-5 OS=Spinacia oleracea PE=2 SV=1)

HSP 1 Score: 253.832 bits (647), Expect = 6.698e-95
Identity = 130/162 (80.25%), Postives = 135/162 (83.33%), Query Frame = 1

Query:   64 MKLDTSGLESTSPFFGSGDELCPEXXXXXXXXXXXXTDFDEFQKDTKATLKHAKGTTTLAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRLHELANKRRISVTGASKLLANILYSYRGMGLSVGTMIAGWDET 549
            MKLDTSGLEST+P F   D +               TDFD FQK+    +K AKGTTTLAFIFK GVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRLHELANKRRISVTGASKLLANILY+YRGMGLSVGTMIAGWDET
Sbjct:    1 MKLDTSGLESTAPIFRRSDFVFDGLQMTPSFDLPNPTDFDGFQKEAVQMVKPAKGTTTLAFIFKHGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRLHELANKRRISVTGASKLLANILYNYRGMGLSVGTMIAGWDET 162

HSP 2 Score: 98.5969 bits (244), Expect = 6.698e-95
Identity = 45/62 (72.58%), Postives = 50/62 (80.65%), Query Frame = 3

Query:  636 GPGLYYVDSEGGRLKGTRFSVGSGSPYAYGVLDSGYKFDMSIXXXXXXXXXXIYHATFRDAA 821
            GPGLYYVDSEGGRLKG RFSVGSGSPYAYGVLD+GYK+DM++          IYHAT+RD A
Sbjct:  163 GPGLYYVDSEGGRLKGMRFSVGSGSPYAYGVLDNGYKYDMTVEEASELARRAIYHATYRDGA 224

HSP 3 Score: 38.891 bits (89), Expect = 6.698e-95
Identity = 13/18 (72.22%), Postives = 17/18 (94.44%), Query Frame = 1

Query:  865 WKKLSGDDVGELHYHYYP 918
            WKK++GDDVG+LH+ YYP
Sbjct:  239 WKKVTGDDVGDLHFQYYP 256

[Close]

BLAST of CK935606 vs. ExPASy Swiss-Prot
Match: PSB5_MOUSE (Proteasome subunit beta type-5 OS=Mus musculus GN=Psmb5 PE=1 SV=3)

HSP 1 Score: 162.925 bits (411), Expect = 1.198e-57
Identity = 77/107 (71.96%), Postives = 91/107 (85.05%), Query Frame = 1

Query:  226 GTTTLAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRLHELANKRRISVTGASKLLANILYSYRGMGLSVGTMIAGWDE 546
            GTTTLAF F  GV+VAADSRA+ G YI+SQ+VKK+IEINPY+LGTMAGGAADC FW R L  +CR++EL NK RISV  ASKLLAN++Y Y+GMGLS+GTMI GWD+
Sbjct:   59 GTTTLAFKFLHGVIVAADSRATAGAYIASQTVKKVIEINPYLLGTMAGGAADCSFWERLLARQCRIYELRNKERISVAAASKLLANMVYQYKGMGLSMGTMICGWDK 165

HSP 2 Score: 82.4185 bits (202), Expect = 1.198e-57
Identity = 37/62 (59.68%), Postives = 44/62 (70.97%), Query Frame = 3

Query:  633 QGPGLYYVDSEGGRLKGTRFSVGSGSPYAYGVLDSGYKFDMSIXXXXXXXXXXIYHATFRDA 818
            +GPGLYYVDSEG R+ GT FSVGSGS YAYGV+D GY +D+ +          IY AT+RDA
Sbjct:  166 RGPGLYYVDSEGNRISGTAFSVGSGSVYAYGVMDRGYSYDLKVEEAYDLARRAIYQATYRDA 227

[Close]

BLAST of CK935606 vs. ExPASy Swiss-Prot
Match: PSB5_PONAB (Proteasome subunit beta type-5 OS=Pongo abelii GN=PSMB5 PE=2 SV=3)

HSP 1 Score: 164.466 bits (415), Expect = 1.561e-57
Identity = 77/107 (71.96%), Postives = 92/107 (85.98%), Query Frame = 1

Query:  226 GTTTLAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRLHELANKRRISVTGASKLLANILYSYRGMGLSVGTMIAGWDE 546
            GTTTLAF F+ GV+VAADSRA+ G YI+SQ+VKK+IEINPY+LGTMAGGAADC FW R L  +CR++EL NK RISV  ASKLLAN++Y Y+GMGLS+GTMI GWD+
Sbjct:   59 GTTTLAFKFRHGVIVAADSRATAGAYIASQTVKKVIEINPYLLGTMAGGAADCSFWERLLARQCRIYELRNKERISVAAASKLLANMVYQYKGMGLSMGTMICGWDK 165

HSP 2 Score: 80.4925 bits (197), Expect = 1.561e-57
Identity = 36/62 (58.06%), Postives = 43/62 (69.35%), Query Frame = 3

Query:  633 QGPGLYYVDSEGGRLKGTRFSVGSGSPYAYGVLDSGYKFDMSIXXXXXXXXXXIYHATFRDA 818
            +GPGLYYVDSEG R+ G  FSVGSGS YAYGV+D GY +D+ +          IY AT+RDA
Sbjct:  166 RGPGLYYVDSEGNRISGATFSVGSGSVYAYGVMDRGYSYDLEVEQAYDLARRAIYQATYRDA 227

[Close]

BLAST of CK935606 vs. ExPASy Swiss-Prot
Match: PSB5_HUMAN (Proteasome subunit beta type-5 OS=Homo sapiens GN=PSMB5 PE=1 SV=3)

HSP 1 Score: 164.466 bits (415), Expect = 1.561e-57
Identity = 77/107 (71.96%), Postives = 92/107 (85.98%), Query Frame = 1

Query:  226 GTTTLAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRLHELANKRRISVTGASKLLANILYSYRGMGLSVGTMIAGWDE 546
            GTTTLAF F+ GV+VAADSRA+ G YI+SQ+VKK+IEINPY+LGTMAGGAADC FW R L  +CR++EL NK RISV  ASKLLAN++Y Y+GMGLS+GTMI GWD+
Sbjct:   59 GTTTLAFKFRHGVIVAADSRATAGAYIASQTVKKVIEINPYLLGTMAGGAADCSFWERLLARQCRIYELRNKERISVAAASKLLANMVYQYKGMGLSMGTMICGWDK 165

HSP 2 Score: 80.4925 bits (197), Expect = 1.561e-57
Identity = 36/62 (58.06%), Postives = 43/62 (69.35%), Query Frame = 3

Query:  633 QGPGLYYVDSEGGRLKGTRFSVGSGSPYAYGVLDSGYKFDMSIXXXXXXXXXXIYHATFRDA 818
            +GPGLYYVDSEG R+ G  FSVGSGS YAYGV+D GY +D+ +          IY AT+RDA
Sbjct:  166 RGPGLYYVDSEGNRISGATFSVGSGSVYAYGVMDRGYSYDLEVEQAYDLARRAIYQATYRDA 227

[Close]

BLAST of CK935606 vs. ExPASy Swiss-Prot
Match: PSB5_BOVIN (Proteasome subunit beta type-5 OS=Bos taurus GN=PSMB5 PE=1 SV=1)

HSP 1 Score: 164.466 bits (415), Expect = 1.561e-57
Identity = 77/107 (71.96%), Postives = 92/107 (85.98%), Query Frame = 1

Query:  226 GTTTLAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRLHELANKRRISVTGASKLLANILYSYRGMGLSVGTMIAGWDE 546
            GTTTLAF F+ GV+VAADSRA+ G YI+SQ+VKK+IEINPY+LGTMAGGAADC FW R L  +CR++EL NK RISV  ASKLLAN++Y Y+GMGLS+GTMI GWD+
Sbjct:   59 GTTTLAFKFRHGVIVAADSRATAGAYIASQTVKKVIEINPYLLGTMAGGAADCSFWERLLARQCRIYELRNKERISVAAASKLLANMVYQYKGMGLSMGTMICGWDK 165

HSP 2 Score: 80.4925 bits (197), Expect = 1.561e-57
Identity = 36/62 (58.06%), Postives = 43/62 (69.35%), Query Frame = 3

Query:  633 QGPGLYYVDSEGGRLKGTRFSVGSGSPYAYGVLDSGYKFDMSIXXXXXXXXXXIYHATFRDA 818
            +GPGLYYVDSEG R+ G  FSVGSGS YAYGV+D GY +D+ +          IY AT+RDA
Sbjct:  166 RGPGLYYVDSEGNRISGATFSVGSGSVYAYGVMDRGYSYDLEVEEAYDLARRAIYQATYRDA 227

[Close]

BLAST of CK935606 vs. ExPASy Swiss-Prot
Match: PSB5_RAT (Proteasome subunit beta type-5 OS=Rattus norvegicus GN=Psmb5 PE=1 SV=3)

HSP 1 Score: 163.31 bits (412), Expect = 2.654e-57
Identity = 77/107 (71.96%), Postives = 92/107 (85.98%), Query Frame = 1

Query:  226 GTTTLAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRLHELANKRRISVTGASKLLANILYSYRGMGLSVGTMIAGWDE 546
            GTTTLAF F+ GV+VAADSRA+ G YI+SQ+VKK+IEINPY+LGTMAGGAADC FW R L  +CR++EL NK RISV  ASKLLAN++Y Y+GMGLS+GTMI GWD+
Sbjct:   59 GTTTLAFKFQHGVIVAADSRATAGPYIASQTVKKVIEINPYLLGTMAGGAADCSFWERLLARQCRIYELRNKERISVAAASKLLANMVYQYKGMGLSMGTMICGWDK 165

HSP 2 Score: 80.8777 bits (198), Expect = 2.654e-57
Identity = 36/62 (58.06%), Postives = 44/62 (70.97%), Query Frame = 3

Query:  633 QGPGLYYVDSEGGRLKGTRFSVGSGSPYAYGVLDSGYKFDMSIXXXXXXXXXXIYHATFRDA 818
            +GPGLYYVDSEG R+ GT FSVGSGS YA+GV+D GY +D+ +          IY AT+RDA
Sbjct:  166 RGPGLYYVDSEGNRISGTAFSVGSGSVYAFGVMDRGYSYDLQVEEAYDLARRAIYQATYRDA 227

[Close]

BLAST of CK935606 vs. ExPASy Swiss-Prot
Match: PSB5_DICDI (Proteasome subunit beta type-5 OS=Dictyostelium discoideum GN=psmB5 PE=1 SV=1)

HSP 1 Score: 157.147 bits (396), Expect = 2.025e-55
Identity = 72/113 (63.72%), Postives = 93/113 (82.30%), Query Frame = 1

Query:  211 LKHAKGTTTLAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRLHELANKRRISVTGASKLLANILYSYRGMGLSVGTMIAGWDET 549
            L+ A GTTTLAF++  G++V+ DS+++ G Y++S+SVKK+IEI P +LGTMAGGAADC FW R LG +CRL+EL NK  ISV  ASK+LANI+YSY+G GLS+GTMI GWD+T
Sbjct:   57 LEFAHGTTTLAFVYGGGIIVSVDSKSTQGPYVASRSVKKVIEITPTLLGTMAGGAADCSFWERELGRRCRLYELRNKELISVAAASKILANIVYSYKGYGLSMGTMITGWDKT 169

HSP 2 Score: 76.2554 bits (186), Expect = 2.025e-55
Identity = 36/61 (59.02%), Postives = 43/61 (70.49%), Query Frame = 3

Query:  636 GPGLYYVDSEGGRLKGTRFSVGSGSPYAYGVLDSGYKFDMSIXXXXXXXXXXIYHATFRDA 818
            GP LYYVD++G RL G RFS GSGS YAYGVLD+G+K+DM+           +YHAT RDA
Sbjct:  170 GPQLYYVDNDGTRLHGQRFSCGSGSTYAYGVLDTGFKWDMNDEEAYELGRRAVYHATHRDA 230

HSP 3 Score: 25.409 bits (54), Expect = 2.025e-55
Identity = 9/17 (52.94%), Postives = 12/17 (70.59%), Query Frame = 1

Query:  865 WKKLSGDDVGELHYHYY 915
            WKK+S DD  +L+  YY
Sbjct:  246 WKKISSDDCFKLYQKYY 262

[Close]

BLAST of CK935606 vs. ExPASy Swiss-Prot
Match: PSB5_SCHPO (Proteasome subunit beta type-5 OS=Schizosaccharomyces pombe GN=pts1 PE=2 SV=3)

HSP 1 Score: 152.14 bits (383), Expect = 7.451e-51
Identity = 72/115 (62.61%), Postives = 92/115 (80.00%), Query Frame = 1

Query:  193 KDTKATLKHAKGTTTLAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRLHELANKRRISVTGASKLLANILYSYRGMGLSVGTMIAG 537
            K+    +K   GTTTLAF ++ G++V  DSRAS G  I+SQ+VKK+IEINPY+LGT+AGGAADCQFW   LG++CRLH+L NK  ISV+ ASK+L+NI YSY+G GLS+GTM+AG
Sbjct:   50 KNKHCLIKMNHGTTTLAFRYQHGIVVCVDSRASAGPLIASQTVKKVIEINPYLLGTLAGGAADCQFWETVLGMECRLHQLRNKELISVSAASKILSNITYSYKGYGLSMGTMLAG 164

HSP 2 Score: 70.4774 bits (171), Expect = 7.451e-51
Identity = 36/64 (56.25%), Postives = 42/64 (65.62%), Query Frame = 3

Query:  627 GLQGPGLYYVDSEGGRLKGTRFSVGSGSPYAYGVLDSGYKFDMSIXXXXXXXXXXIYHATFRDA 818
            G  G  LYY+DS+G RLKG  FSVGSGS +AYGVLDSGY++D+S           I  AT RDA
Sbjct:  166 GKGGTALYYIDSDGTRLKGDLFSVGSGSTFAYGVLDSGYRWDLSKQEALYLAQRSIVAATHRDA 229

The following BLAST results are available for this feature:

BLAST of CK935606 vs. ExPASy Swiss-Prot

BLAST of CK935606 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 31


Match Name	E-value	Identity	Description
PSB5B_ARATH	3.354e-97	79.88	Proteasome subunit beta type-5-B OS=Arabidopsis th...	[more]
PSB5A_ARATH	9.676e-97	78.66	Proteasome subunit beta type-5-A OS=Arabidopsis th...	[more]
PSB5_SPIOL	6.698e-95	80.25	Proteasome subunit beta type-5 OS=Spinacia olerace...	[more]
PSB5_MOUSE	1.198e-57	71.96	Proteasome subunit beta type-5 OS=Mus musculus GN=...	[more]
PSB5_PONAB	1.561e-57	71.96	Proteasome subunit beta type-5 OS=Pongo abelii GN=...	[more]
PSB5_HUMAN	1.561e-57	71.96	Proteasome subunit beta type-5 OS=Homo sapiens GN=...	[more]
PSB5_BOVIN	1.561e-57	71.96	Proteasome subunit beta type-5 OS=Bos taurus GN=PS...	[more]
PSB5_RAT	2.654e-57	71.96	Proteasome subunit beta type-5 OS=Rattus norvegicu...	[more]
PSB5_DICDI	2.025e-55	63.72	Proteasome subunit beta type-5 OS=Dictyostelium di...	[more]
PSB5_SCHPO	7.451e-51	62.61	Proteasome subunit beta type-5 OS=Schizosaccharomy...	[more]

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Properties


Property Name	Value
Genbank description	CGF1004553_F08 Developing fruit 24 DAFB Citrus sinensis cDNA clone T24DAB0004_IIF_F08 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK935606 ID=CK935606; Name=CK935606; organism=Citrus sinensis; type=EST; length=919bp
GGGGGATTTCTGCTTGCAACTCTCAGTTACATTGTCGTTTAATCTTATAT
TCAGTGCGTAGACATGAAGCTCGACACTAGCGGTCTTGAGTCCACTTCTC
CTTTTTTCGGGTCAGGCGACGAGCTCTGTCCTGAGTTATCAGCTGCACCA
TCATTTTCTCTACCTCTTTCTACTGATTTTGATGAATTTCAAAAGGACAC
TAAAGCTACACTGAAGCATGCCAAGGGGACAACCACACTTGCTTTTATCT
TTAAAGAGGGTGTTATGGTGGCTGCTGATTCTCGAGCTAGCATGGGAGGC
TATATCTCATCACAGTCTGTGAAGAAAATTATAGAAATCAATCCTTACAT
GCTTGGCACAATGGCTGGAGGAGCAGCCGATTGCCAGTTTTGGCACAGAA
ATCTGGGCATTAAGTGCCGACTACATGAATTGGCCAACAAGCGTAGAATT
TCAGTTACAGGGGCATCAAAGCTGCTGGCCAACATTCTCTACTCTTATCG
TGGAATGGGGTTGTCTGTTGGGACTATGATTGCTGGATGGGATGAGACTG
TAAGGGAATTCATGTTTCAGGTTTTTTGTTTTGCTTTTGCATCTCTGATT
GCGTCTCTTTTGAGCTGTTTGTCCGTGGGTTGCAGGGTCCTGGGCTGTAT
TATGTGGACAGTGAAGGAGGGAGGCTCAAAGGAACACGGTTCTCTGTGGG
ATCAGGTTCGCCATATGCTTATGGTGTATTGGACAGCGGGTACAAGTTTG
ACATGTCTATTGAAGAAGCTGCGGAGTTGGCTAGAAGAGCTATTTATCAT
GCGACATTCAGAGATGCAGCCAGNTGGTGGTGTTGCAAGCGTTTATTATG
TGGGACNNNNNGGATGGAAGAAGCTGTCTGGTGATGATGTTGGAGAGCTT
CACTACCATTATTATCCAG