Unique Features of a Japanese 'Candidatus Liberibacter asiaticus' Strain Revealed by Whole Genome Sequencing

Publication Overview
TitleUnique Features of a Japanese 'Candidatus Liberibacter asiaticus' Strain Revealed by Whole Genome Sequencing
AuthorsKatoh H, Miyata S, Inoue H, Iwanami T
TypeJournal Article
Journal NamePloS one
Volume9
Issue9
Year2014
Page(s)e106109
CitationKatoh H, Miyata S, Inoue H, Iwanami T. Unique Features of a Japanese 'Candidatus Liberibacter asiaticus' Strain Revealed by Whole Genome Sequencing. PloS one. 2014; 9(9):e106109.

Abstract

Citrus greening (huanglongbing) is the most destructive disease of citrus worldwide. It is spread by citrus psyllids and is associated with phloem-limited bacteria of three species of α-Proteobacteria, namely, 'Candidatus Liberibacter asiaticus', 'Ca. L. americanus', and 'Ca. L. africanus'. Recent findings suggested that some Japanese strains lack the bacteriophage-type DNA polymerase region (DNA pol), in contrast to the Floridian psy62 strain. The whole genome sequence of the pol-negative 'Ca. L. asiaticus' Japanese isolate Ishi-1 was determined by metagenomic analysis of DNA extracted from 'Ca. L. asiaticus'-infected psyllids and leaf midribs. The 1.19-Mb genome has an average 36.32% GC content. Annotation revealed 13 operons encoding rRNA and 44 tRNA genes, but no typical bacterial pathogenesis-related genes were located within the genome, similar to the Floridian psy62 and Chinese gxpsy. In contrast to other 'Ca. L. asiaticus' strains, the genome of the Japanese Ishi-1 strain lacks a prophage-related region.

Properties
Additional details for this publication include:
Property NameValue
Publication ModelElectronic-eCollection
ISSN1932-6203
eISSN1932-6203
Publication Date2014
Journal AbbreviationPLoS ONE
DOI10.1371/journal.pone.0106109
Elocation10.1371/journal.pone.0106109
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountryUnited States
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PMID: PubMedPMID:25180586