Mapping Freeze Tolerance Quantitative Trait Loci in a Citrus grandis × Poncirus trifoliata F1 Pseudo-testcross Using Molecular Markers

Publication Overview
TitleMapping Freeze Tolerance Quantitative Trait Loci in a Citrus grandis × Poncirus trifoliata F1 Pseudo-testcross Using Molecular Markers
AuthorsWeber CA, Moore GA, Deng Z, Gmitter FGJ
TypeJournal Article
Journal NameJournal of the American Society for Horticultural Science
Volume128
Issue4
Year2003
Page(s)508-514
CitationWeber CA, Moore GA, Deng Z, Gmitter FGJ. Mapping Freeze Tolerance Quantitative Trait Loci in a Citrus grandis × Poncirus trifoliata F1 Pseudo-testcross Using Molecular Markers. Journal of the American Society for Horticultural Science. 2003; 128(4):508-514.

Abstract

Mapping quantitative trait loci (QTL) associated with freeze tolerance was accomplished using a Citrus grandis (L.) Osb. × Poncirus trifoliata (L.) Raf. F(1) pseudo-testcross population. A progeny population of 442 plants was acclimated and exposed to temperatures of -9 ÂC and -15 ÂC in two separate freeze tests. A subpopulation of 99 progeny was genotyped for random amplified polymorphic DNA (RAPD), cleaved amplified polymorphic sequence (CAPS), sequence characterized amplified region (SCAR), and sequence tagged site (STS) markers to produce a linkage map for each parent. Potential QTL were identified by interval mapping, and their validity was corroborated with results from means comparison (t test), one-way analysis of variance (F test), and bulked segregant analysis (BSA). Multiple analytical methods provided evidence supporting putative QTL and decreased the probability of missing significant QTL associated with freeze tolerance. QTL with a large effect on freeze tolerance were located on both the Citrus and Poncirus linkage maps. In addition, clusters of markers with significantly different means between marker present and absent classes indicating minor QTL that contribute smaller effects on the level of tolerance were found on the linkage maps of both species.
Featuremaps
This publication contains information about 2 maps:
Map Name
Citrus-DPI6-4/Rubidoux-DPI6-4-F1-2003
Citrus-DPI6-4/Rubidoux-Rubidoux-F1-2003
Stocks
This publication contains information about 4 stocks:
Stock NameUniquenameType
DPI6-4DPI6-4breeding_research_material
RubidouxRubidouxcultivar
Citrus-DPI6-4/Rubidoux-DPI6-4-F1Citrus-DPI6-4/Rubidoux-DPI6-4-F1population
Citrus-DPI6-4/Rubidoux-Rubidoux-F1Citrus-DPI6-4/Rubidoux-Rubidoux-F1population
Properties
Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Publication Date2003
Published Location|||
Language Abbreng
Publication Model[electronic resource].
URLhttp://journal.ashspublications.org/content/128/4.toc
KeywordsCitrus maxima, DNA, Poncirus trifoliata, analysis of variance, analytical methods, chromosome mapping, genetic markers, probability, progeny, quantitative trait loci, random amplified polymorphic DNA technique, t-test, temperature
Cross References
This publication is also available in the following databases:
DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:4767749