The role of CBF transcriptional activators in two Citrus species (Poncirus and Citrus) with contrasting levels of freezing tolerance

Publication Overview
TitleThe role of CBF transcriptional activators in two Citrus species (Poncirus and Citrus) with contrasting levels of freezing tolerance
AuthorsChamp KI, Febres VJ, Moore GA
TypeJournal Article
Journal NamePhysiologia plantarum
Volume129
Issue3
Year2007
Page(s)529-541
CitationChamp KI, Febres VJ, Moore GA. The role of CBF transcriptional activators in two Citrus species (Poncirus and Citrus) with contrasting levels of freezing tolerance. Physiologia plantarum. 2007; 129(3):529-541.

Abstract

Citrus species are considered cold sensitive, yet Poncirus trifoliata, an interfertile Citrus relative, is extremely cold tolerant and can survive freezes at -20°C when fully cold acclimated. To better understand the molecular basis of this difference in freezing tolerance, C-repeat-binding factors (CBFs) from P. trifoliata and Citrus paradisi (grapefruit) were isolated and characterized. Based on our results, P. trifoliata and C. paradisi do contain a CBF pathway for cold-regulated gene expression, and similar to other species, they both appear to contain small CBF families. The nucleotide and amino acid sequences of PtCBF1 and CpCBF1 are highly homologous. However, differences in cold-induced expression were observed. PtCBF1 accumulates both earlier and to higher levels than CpCBF1. Furthermore, CORc115, a cold-induced group II LEA gene, also accumulates earlier and to higher levels in P. trifoliata in response to cold temperatures. The regulatory region of CORc115 contains a putative C-repeat/dehydration-responsive element that is specifically recognized and bound by PtCBF, indicating that CORc115 is a target of PtCBF1. This study provides the first evidence of a correlation between CBF expression (timing and quantity) and the degree of cold tolerance in two closely related species with wide differences in cold tolerance. The importance of using Poncirus and Citrus, two non-model organisms, to address new questions about the CBF pathway is illustrated, as these results provide novel evidence that the CBF expression pattern may play an important role in the notable difference in cold tolerance between these two related species.
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This publication contains information about 26 features:
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Pages

Stocks
This publication contains information about 3 stocks:
Stock NameUniquenameType
Citrus grandisCitrus grandisbreeding_research_material
Citrus paradisiCitrus paradisibreeding_research_material
Poncirus trifoliataPoncirus trifoliatawild_unimproved
Properties
Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Publication Date2007
Published Location|||
Language Abbreng
Publication Model[electronic resource].
URLhttp://dx.doi.org/10.1111/j.1399-3054.2006.00826.x
KeywordsCitrus paradisi, Poncirus trifoliata, amino acid sequences, cold tolerance, freezing, gene expression, genes, grapefruits, species differences
Cross References
This publication is also available in the following databases:
DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:3878333