EY735677

Overview
NameEY735677
Unique NameEY735677
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length900
Libraries
Library NameType
Sweet orange fruit, development stadium (6 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
EY735677_ssr21 microsatellite EY735677_ssr21:21..32. BLAST: Citrus ESTs to Prunus persica proteins V1
Homology
[Close]
BLAST of EY735677 vs. ExPASy Swiss-Prot
Match: ARF1_CATRO (ADP-ribosylation factor 1 OS=Catharanthus roseus GN=ARF1 PE=2 SV=3)

HSP 1 Score: 273.092 bits (697), Expect = 4.766e-78
Identity = 138/150 (92.00%), Postives = 143/150 (95.33%), Query Frame = 2 
Query:   86 MGLSFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVCG*QQDLPNAMNAGEITGQLGLHSLRR 535
            MGLSFTKLFSRLFAKKEMRILMVGLDAAGKTTILY+LKLGEIVTTIPTIGFNVETVEY+ ISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLV   +QDLPNAMNA EIT + GLHSLR+
Sbjct:    1 MGLSFTKLFSRLFAKKEMRILMVGLDAAGKTTILYQLKLGEIVTTIPTIGFNVETVEYQYISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKHGLHSLRQ 150

HSP 2 Score: 40.4318 bits (93), Expect = 4.766e-78
Identity = 15/18 (83.33%), Postives = 17/18 (94.44%), Query Frame = 3 
Query:  540 HWYIHSTCATSGEGVFEG 593
            HWYI STCATSGEG++EG
Sbjct:  152 HWYIQSTCATSGEGLYEG 169
[Close]
BLAST of EY735677 vs. ExPASy Swiss-Prot
Match: ARF_VIGUN (ADP-ribosylation factor OS=Vigna unguiculata GN=ARF PE=2 SV=3)

HSP 1 Score: 281.952 bits (720), Expect = 3.286e-75
Identity = 142/150 (94.67%), Postives = 145/150 (96.67%), Query Frame = 2 
Query:   86 MGLSFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVCG*QQDLPNAMNAGEITGQLGLHSLRR 535
            MGLSFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLV   +QDLPNAMNA EIT +LGLHSLR+
Sbjct:    1 MGLSFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQ 150
[Close]
BLAST of EY735677 vs. ExPASy Swiss-Prot
Match: ARF2_ARATH (ADP-ribosylation factor 2 OS=Arabidopsis thaliana GN=ARF2-A PE=2 SV=3)

HSP 1 Score: 280.026 bits (715), Expect = 1.249e-74
Identity = 141/150 (94.00%), Postives = 144/150 (96.00%), Query Frame = 2 
Query:   86 MGLSFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVCG*QQDLPNAMNAGEITGQLGLHSLRR 535
            MGLSF KLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLV   +QDLPNAMNA EIT +LGLHSLR+
Sbjct:    1 MGLSFAKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQ 150
[Close]
BLAST of EY735677 vs. ExPASy Swiss-Prot
Match: ARF1_DAUCA (ADP-ribosylation factor 1 OS=Daucus carota GN=ARF1 PE=2 SV=2)

HSP 1 Score: 279.641 bits (714), Expect = 1.631e-74
Identity = 141/150 (94.00%), Postives = 144/150 (96.00%), Query Frame = 2 
Query:   86 MGLSFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVCG*QQDLPNAMNAGEITGQLGLHSLRR 535
            MGLSFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLV   +QDLPNAMNA EI  +LGLHSLR+
Sbjct:    1 MGLSFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEIIDKLGLHSLRQ 150
[Close]
BLAST of EY735677 vs. ExPASy Swiss-Prot
Match: ARF2_ORYSJ (ADP-ribosylation factor 2 OS=Oryza sativa subsp. japonica GN=ARF PE=2 SV=2)

HSP 1 Score: 279.256 bits (713), Expect = 2.130e-74
Identity = 140/150 (93.33%), Postives = 145/150 (96.67%), Query Frame = 2 
Query:   86 MGLSFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVCG*QQDLPNAMNAGEITGQLGLHSLRR 535
            MGL+FTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+RVVEARDELHRMLNEDELRDAVLLV   +QDLPNAMNA EIT +LGLHSLR+
Sbjct:    1 MGLTFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQ 150
[Close]
BLAST of EY735677 vs. ExPASy Swiss-Prot
Match: ARF1_ORYSJ (ADP-ribosylation factor 1 OS=Oryza sativa subsp. japonica GN=Os01g0813400 PE=2 SV=3)

HSP 1 Score: 279.256 bits (713), Expect = 2.130e-74
Identity = 140/150 (93.33%), Postives = 145/150 (96.67%), Query Frame = 2 
Query:   86 MGLSFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVCG*QQDLPNAMNAGEITGQLGLHSLRR 535
            MGL+FTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+RVVEARDELHRMLNEDELRDAVLLV   +QDLPNAMNA EIT +LGLHSLR+
Sbjct:    1 MGLTFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQ 150
[Close]
BLAST of EY735677 vs. ExPASy Swiss-Prot
Match: ARF1_ARATH (ADP-ribosylation factor 1 OS=Arabidopsis thaliana GN=ARF1 PE=1 SV=2)

HSP 1 Score: 279.256 bits (713), Expect = 2.130e-74
Identity = 141/150 (94.00%), Postives = 144/150 (96.00%), Query Frame = 2 
Query:   86 MGLSFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVCG*QQDLPNAMNAGEITGQLGLHSLRR 535
            MGLSF KLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLV   +QDLPNAMNA EIT +LGLHSLR+
Sbjct:    1 MGLSFGKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQ 150
[Close]
BLAST of EY735677 vs. ExPASy Swiss-Prot
Match: ARF_MAIZE (ADP-ribosylation factor OS=Zea mays GN=ARF1 PE=2 SV=2)

HSP 1 Score: 278.1 bits (710), Expect = 4.744e-74
Identity = 140/150 (93.33%), Postives = 145/150 (96.67%), Query Frame = 2 
Query:   86 MGLSFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVCG*QQDLPNAMNAGEITGQLGLHSLRR 535
            MGL+FTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLV   +QDLPNAMNA EIT +LGL+SLR+
Sbjct:    1 MGLTFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLNSLRQ 150
[Close]
BLAST of EY735677 vs. ExPASy Swiss-Prot
Match: ARF1_SALBA (ADP-ribosylation factor 1 OS=Salix bakko GN=ARF1 PE=2 SV=3)

HSP 1 Score: 274.248 bits (700), Expect = 6.851e-73
Identity = 138/150 (92.00%), Postives = 142/150 (94.67%), Query Frame = 2 
Query:   86 MGLSFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVCG*QQDLPNAMNAGEITGQLGLHSLRR 535
            MGLSFTKL  RLF+KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRV EARDELHRMLNEDELRDAVLLV   +QDLPNAMNA EIT +LGLHSLR+
Sbjct:    1 MGLSFTKLLGRLFSKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVGEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQ 150
[Close]
BLAST of EY735677 vs. ExPASy Swiss-Prot
Match: ARF1_CHLRE (ADP-ribosylation factor 1 OS=Chlamydomonas reinhardtii GN=ARF1 PE=2 SV=2)

HSP 1 Score: 272.707 bits (696), Expect = 1.993e-72
Identity = 137/150 (91.33%), Postives = 141/150 (94.00%), Query Frame = 2 
Query:   86 MGLSFTKLFSRLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVCG*QQDLPNAMNAGEITGQLGLHSLRR 535
            MGL FTK  SRLF KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+RVVEARDELHRMLNEDELRDAVLLV   +QDLPNAMNA EIT +LGLHSLR+
Sbjct:    1 MGLRFTKALSRLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQ 150
The following BLAST results are available for this feature:
BLAST of EY735677 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 217
Match NameE-valueIdentityDescription
ARF1_CATRO4.766e-7892.00ADP-ribosylation factor 1 OS=Catharanthus roseus G... [more]
ARF_VIGUN3.286e-7594.67ADP-ribosylation factor OS=Vigna unguiculata GN=AR... [more]
ARF2_ARATH1.249e-7494.00ADP-ribosylation factor 2 OS=Arabidopsis thaliana ... [more]
ARF1_DAUCA1.631e-7494.00ADP-ribosylation factor 1 OS=Daucus carota GN=ARF1... [more]
ARF2_ORYSJ2.130e-7493.33ADP-ribosylation factor 2 OS=Oryza sativa subsp. j... [more]
ARF1_ORYSJ2.130e-7493.33ADP-ribosylation factor 1 OS=Oryza sativa subsp. j... [more]
ARF1_ARATH2.130e-7494.00ADP-ribosylation factor 1 OS=Arabidopsis thaliana ... [more]
ARF_MAIZE4.744e-7493.33ADP-ribosylation factor OS=Zea mays GN=ARF1 PE=2 S... [more]
ARF1_SALBA6.851e-7392.00ADP-ribosylation factor 1 OS=Salix bakko GN=ARF1 P... [more]
ARF1_CHLRE1.993e-7291.33ADP-ribosylation factor 1 OS=Chlamydomonas reinhar... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-705-003-F01-CT.F Sweet orange fruit, development stadium (6 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY735677 ID=EY735677; Name=EY735677; organism=Citrus sinensis; type=EST; length=900bp
CACGCGTCCGGTATTTTTATAGAGAGAGAGAGGGGCAGTAGATCTGCACC
AAGGCAAAAACCGCTCTCTTCCTCAGTTGTGAAAAATGGGGCTGTCTTTC
ACCAAGCTTTTCAGCAGGCTTTTTGCTAAGAAAGAGATGAGAATTCTCAT
GGTAGGTCTCGATGCAGCTGGTAAGACTACTATCCTGTACAAGCTTAAGC
TCGGAGAGATCGTCACCACCATTCCTACCATTGGATTTAATGTAGAGACC
GTTGAGTATAAGAACATCAGCTTTACTGTCTGGGATGTCGGGGGTCAAGA
CAAGATCCGACCTTTGTGGAGACATTATTTCCAAAACACCCAAGGACTCA
TCTTTGTGGTTGACAGTAATGATCGCGACCGTGTGGTAGAAGCTAGGGAT
GAGTTGCACAGAATGTTGAATGAGGATGAATTGAGAGATGCTGTGCTGCT
TGTATGTGGCTAACAGCAAGATCTTCCAAATGCAATGAATGCTGGTGAGA
TAACTGGACAGCTTGGCCTTCACTCTCTTCGGCGGGGCCCATTGGTATAT
CCATAGCACCTGTGCCACTTCTGGTGAAGGGGTGTTTGAGGGGACTTGAC
TGGCGTCGGGGAGGGGTGGGGGGGTAGGGGTGTGACCACTCCACTTTTTA
TTTGTGGGTTGGCGGTAGCGGGCGAAAGACCAGAGTCATTATGTGGTTTT
TGGTGGTTCTTGTGTTTGCACCCGCCGGATTATGTGGGGTGGTCGCGGTT
TCTGTGGAGCTGGCTGTGTCCGCTGTCGTCGTGTCTGGGATTGTGTTGCG
GTGCTGCTGGGTTAGGACGCCCGGCACGCCTGTCACCAGCCCGGGAAGAC
TCGAAGCGGTGTGGAGGCCTGAGAGCATCCCCACGGGCAGCCCGGGGGCG
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