EY732183

Overview
NameEY732183
Unique NameEY732183
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length861
Libraries
Library NameType
Sweet orange fruit, development stadium (5 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY732183 vs. ExPASy Swiss-Prot
Match: PP1_ORYSJ (Serine/threonine-protein phosphatase PP1 OS=Oryza sativa subsp. japonica GN=Os03g0268000 PE=2 SV=2)

HSP 1 Score: 173.711 bits (439), Expect = 6.815e-47
Identity = 79/94 (84.04%), Postives = 89/94 (94.68%), Query Frame = 2 
Query:   62 DFRGWEANDRGVSYVFGADRVTEFLQKHDLELICRAHQVVEDGYEFFANRRLITIFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPADKKPKF 343
            D +GW  NDRGVSY FGAD+V+EFL+KHDL+LICRAHQVVEDGYEFFA+R+L+TIFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPA++K KF
Sbjct:  223 DVQGWGMNDRGVSYTFGADKVSEFLEKHDLDLICRAHQVVEDGYEFFADRQLVTIFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPAERKGKF 316

HSP 2 Score: 33.4982 bits (75), Expect = 6.815e-47
Identity = 12/14 (85.71%), Postives = 14/14 (100.00%), Query Frame = 1 
Query:   13 PESGLLCDLLWSDP 54
            P++GLLCDLLWSDP
Sbjct:  207 PDTGLLCDLLWSDP 220
[Close]
BLAST of EY732183 vs. ExPASy Swiss-Prot
Match: PP1_EMENI (Serine/threonine-protein phosphatase PP1 OS=Emericella nidulans GN=bimG PE=2 SV=1)

HSP 1 Score: 171.785 bits (434), Expect = 3.340e-46
Identity = 77/115 (66.96%), Postives = 95/115 (82.61%), Query Frame = 2 
Query:   53 PIIDFRGWEANDRGVSYVFGADRVTEFLQKHDLELICRAHQVVEDGYEFFANRRLITIFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPADKKPKFSFGSTTTVKSGAPPSRMK 397
            P  D  GW  NDRGVS+ FG D V+ FLQKHD++LICRAHQVVEDGYEFF+ R+L+T+FSAPNYCGEFDNAGAMMSVDE+L+CSFQILKPA+KK K+ +G+ ++ +   PP + K
Sbjct:  208 PDKDITGWSENDRGVSFTFGPDVVSRFLQKHDMDLICRAHQVVEDGYEFFSKRQLVTLFSAPNYCGEFDNAGAMMSVDESLLCSFQILKPAEKKQKYVYGAMSSGRPITPPRKQK 322

HSP 2 Score: 33.113 bits (74), Expect = 3.340e-46
Identity = 12/15 (80.00%), Postives = 14/15 (93.33%), Query Frame = 1 
Query:   13 PESGLLCDLLWSDPN 57
            P+ GLLCDLLWSDP+
Sbjct:  195 PDCGLLCDLLWSDPD 209
[Close]
BLAST of EY732183 vs. ExPASy Swiss-Prot
Match: PP13_ARATH (Serine/threonine-protein phosphatase PP1 isozyme 3 OS=Arabidopsis thaliana GN=TOPP3 PE=2 SV=1)

HSP 1 Score: 171.785 bits (434), Expect = 3.340e-46
Identity = 81/113 (71.68%), Postives = 91/113 (80.53%), Query Frame = 2 
Query:   53 PIIDFRGWEANDRGVSYVFGADRVTEFLQKHDLELICRAHQVVEDGYEFFANRRLITIFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPADKKPKFSFGSTTTVKSGAPPSR 391
            P  D  GW  NDRGVSY FGAD+V EFLQ HDL+LICRAHQVVEDGYEFFANR+L+TIFSAPNYCGEFDNAGAMMSVD++L CSFQILK ++KK  F FG     + G PP +
Sbjct:  206 PDKDIEGWGENDRGVSYTFGADKVEEFLQTHDLDLICRAHQVVEDGYEFFANRQLVTIFSAPNYCGEFDNAGAMMSVDDSLTCSFQILKASEKKGNFGFGKNAG-RRGTPPRK 317

HSP 2 Score: 33.113 bits (74), Expect = 3.340e-46
Identity = 12/15 (80.00%), Postives = 14/15 (93.33%), Query Frame = 1 
Query:   13 PESGLLCDLLWSDPN 57
            P+ GLLCDLLWSDP+
Sbjct:  193 PDHGLLCDLLWSDPD 207
[Close]
BLAST of EY732183 vs. ExPASy Swiss-Prot
Match: PP14_ARATH (Serine/threonine-protein phosphatase PP1 isozyme 4 OS=Arabidopsis thaliana GN=TOPP4 PE=2 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 3.340e-46
Identity = 76/94 (80.85%), Postives = 86/94 (91.49%), Query Frame = 2 
Query:   62 DFRGWEANDRGVSYVFGADRVTEFLQKHDLELICRAHQVVEDGYEFFANRRLITIFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPADKKPKF 343
            D +GW  NDRGVSY FG D+V+EFL KHDL+L+CRAHQVVEDGYEFFA+R+L+T+FSAPNYCGEFDNAGAMMSVDE LMCSFQILKPA+KK KF
Sbjct:  222 DVKGWGMNDRGVSYTFGPDKVSEFLTKHDLDLVCRAHQVVEDGYEFFADRQLVTVFSAPNYCGEFDNAGAMMSVDENLMCSFQILKPAEKKTKF 315

HSP 2 Score: 35.039 bits (79), Expect = 3.340e-46
Identity = 13/18 (72.22%), Postives = 16/18 (88.89%), Query Frame = 1 
Query:    1 PRVRPESGLLCDLLWSDP 54
            P + P++GLLCDLLWSDP
Sbjct:  202 PTMIPDTGLLCDLLWSDP 219
[Close]
BLAST of EY732183 vs. ExPASy Swiss-Prot
Match: PP1B_XENTR (Serine/threonine-protein phosphatase PP1-beta catalytic subunit OS=Xenopus tropicalis GN=ppp1cb PE=2 SV=1)

HSP 1 Score: 170.629 bits (431), Expect = 4.352e-46
Identity = 77/111 (69.37%), Postives = 92/111 (82.88%), Query Frame = 2 
Query:   53 PIIDFRGWEANDRGVSYVFGADRVTEFLQKHDLELICRAHQVVEDGYEFFANRRLITIFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPADKKPKFSFGSTTTVKSGAPP 385
            P  D +GW  NDRGVS+ FGAD V++FL +HDL+LICRAHQVVEDGYEFFA R+L+T+FSAPNYCGEFDNAG MMSVDETLMCSFQILKP++KK K+ +G   + +   PP
Sbjct:  208 PDKDVQGWGENDRGVSFTFGADVVSKFLNRHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGGMMSVDETLMCSFQILKPSEKKAKYQYGGLNSGRPVTPP 318

HSP 2 Score: 33.8834 bits (76), Expect = 4.352e-46
Identity = 12/15 (80.00%), Postives = 15/15 (100.00%), Query Frame = 1 
Query:   13 PESGLLCDLLWSDPN 57
            P++GLLCDLLWSDP+
Sbjct:  195 PDTGLLCDLLWSDPD 209
[Close]
BLAST of EY732183 vs. ExPASy Swiss-Prot
Match: PP1B_XENLA (Serine/threonine-protein phosphatase PP1-beta catalytic subunit OS=Xenopus laevis GN=ppp1cb PE=2 SV=1)

HSP 1 Score: 170.629 bits (431), Expect = 4.352e-46
Identity = 77/111 (69.37%), Postives = 92/111 (82.88%), Query Frame = 2 
Query:   53 PIIDFRGWEANDRGVSYVFGADRVTEFLQKHDLELICRAHQVVEDGYEFFANRRLITIFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPADKKPKFSFGSTTTVKSGAPP 385
            P  D +GW  NDRGVS+ FGAD V++FL +HDL+LICRAHQVVEDGYEFFA R+L+T+FSAPNYCGEFDNAG MMSVDETLMCSFQILKP++KK K+ +G   + +   PP
Sbjct:  208 PDKDVQGWGENDRGVSFTFGADVVSKFLNRHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGGMMSVDETLMCSFQILKPSEKKAKYQYGGLNSGRPVTPP 318

HSP 2 Score: 33.8834 bits (76), Expect = 4.352e-46
Identity = 12/15 (80.00%), Postives = 15/15 (100.00%), Query Frame = 1 
Query:   13 PESGLLCDLLWSDPN 57
            P++GLLCDLLWSDP+
Sbjct:  195 PDTGLLCDLLWSDPD 209
[Close]
BLAST of EY732183 vs. ExPASy Swiss-Prot
Match: PP1B_RAT (Serine/threonine-protein phosphatase PP1-beta catalytic subunit OS=Rattus norvegicus GN=Ppp1cb PE=1 SV=3)

HSP 1 Score: 170.629 bits (431), Expect = 4.352e-46
Identity = 77/111 (69.37%), Postives = 92/111 (82.88%), Query Frame = 2 
Query:   53 PIIDFRGWEANDRGVSYVFGADRVTEFLQKHDLELICRAHQVVEDGYEFFANRRLITIFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPADKKPKFSFGSTTTVKSGAPP 385
            P  D +GW  NDRGVS+ FGAD V++FL +HDL+LICRAHQVVEDGYEFFA R+L+T+FSAPNYCGEFDNAG MMSVDETLMCSFQILKP++KK K+ +G   + +   PP
Sbjct:  208 PDKDVQGWGENDRGVSFTFGADVVSKFLNRHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGGMMSVDETLMCSFQILKPSEKKAKYQYGGLNSGRPVTPP 318

HSP 2 Score: 33.8834 bits (76), Expect = 4.352e-46
Identity = 12/15 (80.00%), Postives = 15/15 (100.00%), Query Frame = 1 
Query:   13 PESGLLCDLLWSDPN 57
            P++GLLCDLLWSDP+
Sbjct:  195 PDTGLLCDLLWSDPD 209
[Close]
BLAST of EY732183 vs. ExPASy Swiss-Prot
Match: PP1B_RABIT (Serine/threonine-protein phosphatase PP1-beta catalytic subunit OS=Oryctolagus cuniculus GN=PPP1CB PE=1 SV=3)

HSP 1 Score: 170.629 bits (431), Expect = 4.352e-46
Identity = 77/111 (69.37%), Postives = 92/111 (82.88%), Query Frame = 2 
Query:   53 PIIDFRGWEANDRGVSYVFGADRVTEFLQKHDLELICRAHQVVEDGYEFFANRRLITIFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPADKKPKFSFGSTTTVKSGAPP 385
            P  D +GW  NDRGVS+ FGAD V++FL +HDL+LICRAHQVVEDGYEFFA R+L+T+FSAPNYCGEFDNAG MMSVDETLMCSFQILKP++KK K+ +G   + +   PP
Sbjct:  208 PDKDVQGWGENDRGVSFTFGADVVSKFLNRHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGGMMSVDETLMCSFQILKPSEKKAKYQYGGLNSGRPVTPP 318

HSP 2 Score: 33.8834 bits (76), Expect = 4.352e-46
Identity = 12/15 (80.00%), Postives = 15/15 (100.00%), Query Frame = 1 
Query:   13 PESGLLCDLLWSDPN 57
            P++GLLCDLLWSDP+
Sbjct:  195 PDTGLLCDLLWSDPD 209
[Close]
BLAST of EY732183 vs. ExPASy Swiss-Prot
Match: PP1B_PONAB (Serine/threonine-protein phosphatase PP1-beta catalytic subunit OS=Pongo abelii GN=PPP1CB PE=2 SV=1)

HSP 1 Score: 170.629 bits (431), Expect = 4.352e-46
Identity = 77/111 (69.37%), Postives = 92/111 (82.88%), Query Frame = 2 
Query:   53 PIIDFRGWEANDRGVSYVFGADRVTEFLQKHDLELICRAHQVVEDGYEFFANRRLITIFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPADKKPKFSFGSTTTVKSGAPP 385
            P  D +GW  NDRGVS+ FGAD V++FL +HDL+LICRAHQVVEDGYEFFA R+L+T+FSAPNYCGEFDNAG MMSVDETLMCSFQILKP++KK K+ +G   + +   PP
Sbjct:  208 PDKDVQGWGENDRGVSFTFGADVVSKFLNRHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGGMMSVDETLMCSFQILKPSEKKAKYQYGGLNSGRPVTPP 318

HSP 2 Score: 33.8834 bits (76), Expect = 4.352e-46
Identity = 12/15 (80.00%), Postives = 15/15 (100.00%), Query Frame = 1 
Query:   13 PESGLLCDLLWSDPN 57
            P++GLLCDLLWSDP+
Sbjct:  195 PDTGLLCDLLWSDPD 209
[Close]
BLAST of EY732183 vs. ExPASy Swiss-Prot
Match: PP1B_PIG (Serine/threonine-protein phosphatase PP1-beta catalytic subunit OS=Sus scrofa GN=PPP1CB PE=1 SV=3)

HSP 1 Score: 170.629 bits (431), Expect = 4.352e-46
Identity = 77/111 (69.37%), Postives = 92/111 (82.88%), Query Frame = 2 
Query:   53 PIIDFRGWEANDRGVSYVFGADRVTEFLQKHDLELICRAHQVVEDGYEFFANRRLITIFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPADKKPKFSFGSTTTVKSGAPP 385
            P  D +GW  NDRGVS+ FGAD V++FL +HDL+LICRAHQVVEDGYEFFA R+L+T+FSAPNYCGEFDNAG MMSVDETLMCSFQILKP++KK K+ +G   + +   PP
Sbjct:  208 PDKDVQGWGENDRGVSFTFGADVVSKFLNRHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGGMMSVDETLMCSFQILKPSEKKAKYQYGGLNSGRPVTPP 318

HSP 2 Score: 33.8834 bits (76), Expect = 4.352e-46
Identity = 12/15 (80.00%), Postives = 15/15 (100.00%), Query Frame = 1 
Query:   13 PESGLLCDLLWSDPN 57
            P++GLLCDLLWSDP+
Sbjct:  195 PDTGLLCDLLWSDPD 209
The following BLAST results are available for this feature:
BLAST of EY732183 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 164
Match NameE-valueIdentityDescription
PP1_ORYSJ6.815e-4784.04Serine/threonine-protein phosphatase PP1 OS=Oryza ... [more]
PP1_EMENI3.340e-4666.96Serine/threonine-protein phosphatase PP1 OS=Emeric... [more]
PP13_ARATH3.340e-4671.68Serine/threonine-protein phosphatase PP1 isozyme 3... [more]
PP14_ARATH3.340e-4680.85Serine/threonine-protein phosphatase PP1 isozyme 4... [more]
PP1B_XENTR4.352e-4669.37Serine/threonine-protein phosphatase PP1-beta cata... [more]
PP1B_XENLA4.352e-4669.37Serine/threonine-protein phosphatase PP1-beta cata... [more]
PP1B_RAT4.352e-4669.37Serine/threonine-protein phosphatase PP1-beta cata... [more]
PP1B_RABIT4.352e-4669.37Serine/threonine-protein phosphatase PP1-beta cata... [more]
PP1B_PONAB4.352e-4669.37Serine/threonine-protein phosphatase PP1-beta cata... [more]
PP1B_PIG4.352e-4669.37Serine/threonine-protein phosphatase PP1-beta cata... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-704-056-H05-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY732183 ID=EY732183; Name=EY732183; organism=Citrus sinensis; type=EST; length=861bp
CCACGCGTCCGCCCAGAAAGTGGTTTGCTCTGCGATCTCCTATGGTCGGA
TCCCAATCATCGACTTTCGAGGTTGGGAAGCAAATGATAGAGGAGTTTCA
TATGTCTTTGGTGCTGATAGAGTGACAGAGTTTCTTCAGAAGCACGATCT
CGAGTTAATTTGTCGGGCCCACCAGGTTGTGGAAGATGGATATGAATTTT
TTGCTAATAGACGGCTCATAACTATATTTTCAGCACCTAATTACTGTGGA
GAGTTTGACAATGCTGGTGCAATGATGAGCGTGGATGAGACACTAATGTG
TTCTTTTCAGATATTAAAGCCTGCAGATAAGAAGCCAAAGTTCAGTTTTG
GGAGCACGACTACAGTTAAGTCAGGTGCTCCTCCAAGTAGAATGAAGTCG
TTTCTTGGTGCAAAGGTTTAATAAGCTTGATCTAAAATGGAGCTTTCTGA
AGAAAGTAGCTGTATTAGATCAATGATGTATAGAACATCACGAGAAATAG
CAATCTAACAAATCTGGGTTCCAATAGTACGTCCCAACGAGGCAAACCGT
TGGTGTTGTCAACCTTGTTGAGCAGCAGAGGATAAATGATGTGCCTCCAG
AAAATTTGTGCGGAAGATGTAATCTCTTGTTGTTTTGCTCCCCCTCTGTA
CATTATGAGTGCTACACCAGCTGGAAGTGGTGGCCTCTCGGGACTCCCCT
CTGGTCGCTTCTTCCTTAGAACCGGGTCGGATCATAATCCGCGCGGGGGG
GATTCACGGGGGTGGTTGGTTGAGGTCGGCGTTTCGGGGCGACTCTTGTG
AGGGTCCTCCGTGTCGCGCGGCGATGGTCAGGCGGGGGGGCCTCCGCTCC
GCAGTCGCCGC
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