EY756224

Overview
NameEY756224
Unique NameEY756224
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length884
Libraries
Library NameType
Sweet orange leaf, field plant Bcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of EY756224 vs. ExPASy Swiss-Prot
Match: GSTU6_ORYSJ (Probable glutathione S-transferase GSTU6 OS=Oryza sativa subsp. japonica GN=GSTU6 PE=2 SV=2)

HSP 1 Score: 180.644 bits (457), Expect = 1.003e-44
Identity = 95/192 (49.48%), Postives = 123/192 (64.06%), Query Frame = 3
Query:   12 KSELLLKSNPVHKKIPVLIHNDKPVCESLIIVEYVDEAWXXXXXXXXXX---DPYDRAVARFWAAYVDEKLFPALKSIGSGDGAEAKKAA-IEQLIEGLVLLEDAFVKCSKGKPFFGGSQIGFLDIAFGCYLGWVRVTEKMNEVKLLDEVKTPGLFKWAERFCADAAVKDVMP-ETDKLAEFAKVLAKLRAS 572
            KS+LLL SNPVHK +PVL+H  +PV ES +IV+Y+DE W             DPY+RAVARFWAAYVD+K+ PA  +I  G   E ++AA + Q +  L  LE AF +CSKGKPFFGG  +GF+D+  G YLGW    +K+   +L+D  +TP L  W ERF A  A K V+P + DKL EF + L +  AS
Sbjct:   42 KSDLLLASNPVHKSVPVLLHAGRPVNESQVIVQYIDEVWPGGAGGRPSVMPSDPYERAVARFWAAYVDDKVRPAWLAILFGSKTEEERAAAVAQAVAALETLEGAFGECSKGKPFFGGDGVGFVDVVLGGYLGWFTAIDKLIGRRLIDPARTPALAAWEERFRATDAAKGVVPDDADKLLEFRQTLLRWSAS 233          
BLAST of EY756224 vs. ExPASy Swiss-Prot
Match: GSTX4_TOBAC (Probable glutathione S-transferase OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 145.206 bits (365), Expect = 4.669e-34
Identity = 80/183 (43.72%), Postives = 104/183 (56.83%), Query Frame = 3
Query:    6 RPKSELLLKSNPVHKKIPVLIHNDKPVCESLIIVEYVDEAWXXXXXXXXXXDPYDRAVARFWAAYVDEKLFPALKSIGSGDGAEAKKAAIEQLIEGLVLLEDAFVKCSKGKPFFGGSQIGFLDIAFGCYLGWVRVTEKMNEVKLLDEVKTPGLFKWAERFCADAAVKDVMPETDKLAEFAKVL 554
            R KS LLL+ NP+HKKIPVLIHN KP+CES+I VEY++E W          DPYDRA ARFWA Y+D+KL+   + + +  G E ++AA +  IE L +LE A       KP+FGG   GF+DIA   Y  W    E         E + P    WA+R     +V   +P+  K+ EF KVL
Sbjct:   39 RNKSPLLLQMNPIHKKIPVLIHNGKPICESIIAVEYIEEVWKDKAPNLLPSDPYDRAQARFWADYIDKKLYDFGRKLWTTKG-EEQEAAKKDFIECLKVLEGAL----GDKPYFGGESFGFVDIALIGYYSWFYAYETFGNFS--TEAECPKFVAWAKRCMQRESVAKSLPDQPKVLEFVKVL 214          
BLAST of EY756224 vs. ExPASy Swiss-Prot
Match: GSTXC_TOBAC (Probable glutathione S-transferase parC OS=Nicotiana tabacum GN=PARC PE=2 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 3.026e-33
Identity = 78/183 (42.62%), Postives = 104/183 (56.83%), Query Frame = 3
Query:    6 RPKSELLLKSNPVHKKIPVLIHNDKPVCESLIIVEYVDEAWXXXXXXXXXXDPYDRAVARFWAAYVDEKLFPALKSIGSGDGAEAKKAAIEQLIEGLVLLEDAFVKCSKGKPFFGGSQIGFLDIAFGCYLGWVRVTEKMNEVKLLDEVKTPGLFKWAERFCADAAVKDVMPETDKLAEFAKVL 554
            R KS LLL+ NP+HKKIPVLIHN KP+CES+I VEY++E W          DPYDRA ARFWA Y+D+KL+   + + +  G E ++AA +  IE L +LE A       +P+FGG   GF+DIA   +  W    E         E + P    WA+R     +V   +P+  K+ EF KVL
Sbjct:   39 RNKSPLLLQMNPIHKKIPVLIHNGKPICESIIAVEYIEEVWKDKAPSLLPSDPYDRAQARFWADYIDKKLYDFGRKLWATKG-EEQEAAKKDFIECLKVLEGAL----GDRPYFGGESFGFVDIALIGFYSWFYAYETFGNFS--TEAECPKFVAWAKRCMQRESVAKSLPDQPKVLEFVKVL 214          
BLAST of EY756224 vs. ExPASy Swiss-Prot
Match: GSTX6_SOYBN (Probable glutathione S-transferase OS=Glycine max GN=HSP26-A PE=2 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 9.735e-32
Identity = 73/180 (40.56%), Postives = 109/180 (60.56%), Query Frame = 3
Query:   12 KSELLLKSNPVHKKIPVLIHNDKPVCESLIIVEYVDEAWXXXXXXXXXXDPYDRAVARFWAAYVDEKLFPAL-KSIGSGDGAEAKKAAIEQLIEGLVLLEDAFVKCSKGKPFFGGSQIGFLDIAFGCYLGWVRVTEKMNEVKLLDEVKTPGLFKWAERFCADAAVKDVMPETDKLAEFAK 548
            KS+LLLK NPVHKK+PV +HN++P+ ESL+IVEY+DE W          DPY RA+ARFW+ ++D+K+  A+ KS+ + D  E +K  +E+  E L  LE+      K K FFGG + G +DIA      W+ + +++  ++L    K P L+KW++ F     V +V+P  D L  + K
Sbjct:   43 KSDLLLKYNPVHKKVPVFVHNEQPIAESLVIVEYIDETW--KNNPILPSDPYQRALARFWSKFIDDKIVGAVSKSVFTVDEKEREK-NVEETYEALQFLENEL----KDKKFFGGEEFGLVDIAAVFIAFWIPIFQEIAGLQLFTSEKFPILYKWSQEFLNHPFVHEVLPPRDPLFAYFK 215          
BLAST of EY756224 vs. ExPASy Swiss-Prot
Match: GSTX2_MAIZE (Probable glutathione S-transferase BZ2 OS=Zea mays GN=BZ2 PE=3 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 2.832e-31
Identity = 73/189 (38.62%), Postives = 111/189 (58.73%), Query Frame = 3
Query:   12 KSELLLKSNPVHKKIPVLIHND-KPVCESLIIVEYVDEA-----WXXXXXXXXXXDPYDRAVARFWAAYVDEKLFPALKSIGSGDGAEAKKAAIEQLIEGLVLLEDAFVKCSKGKPFFGGSQI--GFLDIAFGCYLGWVRVTEKMNEVKLLDEVKTPGLFKWAERFCADAAVKDVMPETDKLAEFAKVL 554
            KS+ LL +NPV+ KIPVL+  D + +CES +IV+Y+++                 DPY+RA+ RFW A++D+K +PAL ++       A+  A E     L LLE+AF   S G+ FF G     G LD+A GC+L  +R  E+++ + L+D   TP L  W++RF A  A K V+P+T+K+ +F + L
Sbjct:   37 KSDRLLAANPVYGKIPVLLLPDGRAICESAVIVQYIEDVARESGGAEAGSLLLPDDPYERAMHRFWTAFIDDKFWPALDAVSLAPTPGARAQAAEDTRAALSLLEEAFKDRSNGRAFFSGGDAAPGLLDLALGCFLPALRACERLHGLSLIDASATPLLDGWSQRFAAHPAAKRVLPDTEKVVQFTRFL 225          
BLAST of EY756224 vs. ExPASy Swiss-Prot
Match: GSTX1_TOBAC (Probable glutathione S-transferase OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 1.455e-27
Identity = 70/178 (39.33%), Postives = 104/178 (58.43%), Query Frame = 3
Query:   12 KSELLLKSNPVHKKIPVLIHNDKPVCESLIIVEYVDEAWXXXXXXXXXXDPYDRAVARFWAAYVDEKLFPALKS-IGSGDGAEAKKAAIEQLIEGLVLLEDAFVKCSKGKPFFGGSQIGFLDIAFGCYLGWVRVTEKMNEVKLLDEVKTPGLFKWAERFCADAAVKDVMPETDKLAEF 542
            KS LLL+SNP+HKK+PVLIHN K + ES++I+EY+DE +          DPYDRA+ARFWA ++D+K+   +K+ +  G+  E  K   E++ E L +L++      K K FF G + GF DIA      W+ V E+ + V L+   K P   KW   +   + +K+ +P  D+L  F
Sbjct:   39 KSSLLLQSNPIHKKVPVLIHNGKRIVESMVILEYIDETF--EGPSILPKDPYDRALARFWAKFLDDKVPAVVKTFLRKGEEQEKDK---EEVCEMLKVLDNEL----KDKKFFVGDKFGFADIAANLVAFWLGVFEEASGVVLVTSEKFPNFCKWRGEYINCSQIKESLPPRDELLAF 207          
BLAST of EY756224 vs. ExPASy Swiss-Prot
Match: GSTXA_TOBAC (Probable glutathione S-transferase parA OS=Nicotiana tabacum GN=PARA PE=2 SV=1)

HSP 1 Score: 121.709 bits (304), Expect = 5.528e-27
Identity = 69/177 (38.98%), Postives = 97/177 (54.80%), Query Frame = 3
Query:   12 KSELLLKSNPVHKKIPVLIHNDKPVCESLIIVEYVDEAWXXXXXXXXXXDPYDRAVARFWAAYVDEKLFPALKSIGSGDGAEAKKAAIEQLIEGLVLLEDAFVKCSKGKPFFGGSQIGFLDIAFGCYLGWVRVTEKMNEVKLLDEVKTPGLFKWAERFCADAAVKDVMPETDKLAEF 542
            KS LLL+ NPVHKKIP+LIHN K +CESL I+EY+DE W          DPY+R+ ARFWA Y+D+K++   + + SG G E ++ A ++ IE L  LE         K +FGG  +GF+D+A   +  W    E      +  E + P L  WA+      +V   +P   K+  F
Sbjct:   41 KSPLLLEMNPVHKKIPILIHNSKAICESLNILEYIDEVW-HDKCPLLPSDPYERSQARFWADYIDKKIYSTGRRVWSGKG-EDQEEAKKEFIEILKTLEGEL----GNKTYFGGDNLGFVDVALVPFTSWFYSYETCANFSI--EAECPKLVVWAKTCMESESVSKSLPHPHKIYGF 209          
BLAST of EY756224 vs. ExPASy Swiss-Prot
Match: GSTX3_TOBAC (Probable glutathione S-transferase OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 1.231e-26
Identity = 74/191 (38.74%), Postives = 109/191 (57.07%), Query Frame = 3
Query:   12 KSELLLKSNPVHKKIPVLIHNDKPVCESLIIVEYVDEAWXXXXXXXXXXDPYDRAVARFWAAYVDEKLFPALKSIGSGDGAEAKKAAIEQLIEGLVLLEDAFVKCSKGKPFFGGSQIGFLDIAFGCYLGWVRVTEKMNEVKLLDEVKTPGLFKWAERFCADAAVKDVMPETDKL-----AEFAKVLAKLRA 569
            KS LLL+SNPVHKK+PVLIHN KP+ ES++I+EY+DE +          DPYDRA+ARFW+ ++ +K+  A+ +     G E +K   E++ E L +L++      K K FF G + GF DIA      W+ V E+   V L+   K P   +W + +   + VK+ +P  D+L     A F  V+A + A
Sbjct:   39 KSSLLLQSNPVHKKVPVLIHNGKPIVESMVILEYIDETF--EGPSILPKDPYDRALARFWSKFLGDKV-AAVVNTFFRKGEEQEKGK-EEVYEMLKVLDNEL----KDKKFFVGDKFGFADIAANLVGFWLGVFEEGYGVVLVTSEKFPNFSRWRDEYINCSQVKESLPSRDELLAFFRARFQAVVASISA 221          
BLAST of EY756224 vs. ExPASy Swiss-Prot
Match: GSTX3_SOYBN (Glutathione S-transferase 3 OS=Glycine max GN=GST3 PE=1 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 3.583e-26
Identity = 64/174 (36.78%), Postives = 92/174 (52.87%), Query Frame = 3
Query:   12 KSELLLKSNPVHKKIPVLIHNDKPVCESLIIVEYVDEAWXXXXXXXXXXDPYDRAVARFWAAYVDEKLFPALKSIGSGDGAEAKKAAIEQLIEGLVLLEDAFVKCSKGKPFFGGSQIGFLDIAFGCYLGWVRVTEKMNEVKLLDEVKTPGLFKWAERFCADAAVKDVMPETDKL 533
            +S LLL+ NP+HKKIPVLIHN KP+CES IIV+Y+DE W          DPY R+ ARFW  Y+D+K++   K +    G E ++   ++LI     LE+        KPF+G    GF+D+    +  W    E     K+  E + P L  W +R      V + +P+  K+
Sbjct:   40 RSPLLLQMNPIHKKIPVLIHNGKPICESAIIVQYIDEVW-NDKSPLMPSDPYKRSQARFWVDYIDKKIYDTWKKMWLSKGEEHEEGK-KELISIFKQLEETLT----DKPFYGDDTFGFVDLCLITFSSWFYTYETYGNFKM--EEECPKLMAWVKRCMERETVSNTLPDAKKV 205          
BLAST of EY756224 vs. ExPASy Swiss-Prot
Match: GSTX2_TOBAC (Probable glutathione S-transferase OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 3.583e-26
Identity = 71/177 (40.11%), Postives = 102/177 (57.63%), Query Frame = 3
Query:   12 KSELLLKSNPVHKKIPVLIHNDKPVCESLIIVEYVDEAWXXXXXXXXXXDPYDRAVARFWAAYVDEKLFPALKSIGSGDGAEAKKAAIEQLIEGLVLLEDAFVKCSKGKPFFGGSQIGFLDIAFGCYLGWVRVTEKMNEVKLLDEVKTPGLFKWAERFCADAAVKDVMPETDKLAEF 542
            KS LLL+SNPV+KK+PVLIHN KP+ ES+II+EY+DE +          DPYDRA+ARFWA ++D+K+  A+ +     G E +K   E++ E L +L++      K K FF G + GF DIA      W+ V E+     L+   K P   KW + +   + V + +P  D+L  F
Sbjct:   39 KSSLLLQSNPVYKKVPVLIHNGKPIVESMIILEYIDETF--EGPSILPKDPYDRALARFWAKFLDDKV-AAVVNTFFRKGEEQEKGK-EEVYEMLKVLDNEL----KDKKFFAGDKFGFADIAANLVGFWLGVFEEGYGDVLVKSEKFPNFSKWRDEYINCSQVNESLPPRDELLAF 207          
The following BLAST results are available for this feature:
BLAST of EY756224 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 15
Match NameE-valueIdentityDescription
GSTU6_ORYSJ1.003e-4449.48Probable glutathione S-transferase GSTU6 OS=Oryza ... [more]
GSTX4_TOBAC4.669e-3443.72Probable glutathione S-transferase OS=Nicotiana ta... [more]
GSTXC_TOBAC3.026e-3342.62Probable glutathione S-transferase parC OS=Nicotia... [more]
GSTX6_SOYBN9.735e-3240.56Probable glutathione S-transferase OS=Glycine max ... [more]
GSTX2_MAIZE2.832e-3138.62Probable glutathione S-transferase BZ2 OS=Zea mays... [more]
GSTX1_TOBAC1.455e-2739.33Probable glutathione S-transferase OS=Nicotiana ta... [more]
GSTXA_TOBAC5.528e-2738.98Probable glutathione S-transferase parA OS=Nicotia... [more]
GSTX3_TOBAC1.231e-2638.74Probable glutathione S-transferase OS=Nicotiana ta... [more]
GSTX3_SOYBN3.583e-2636.78Glutathione S-transferase 3 OS=Glycine max GN=GST3... [more]
GSTX2_TOBAC3.583e-2640.11Probable glutathione S-transferase OS=Nicotiana ta... [more]

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Properties
Property NameValue
Genbank descriptionCS13-C1-001-006-A10-CT.F Sweet orange leaf, field plant B Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY756224 ID=EY756224; Name=EY756224; organism=Citrus sinensis; type=EST; length=884bp
CCACGCGTCCGAAAAGCGAGCTTCTGCTTAAATCAAACCCTGTTCACAAG
AAAATCCCAGTTCTGATTCACAATGATAAGCCTGTTTGCGAATCTCTCAT
CATTGTCGAGTACGTGGATGAGGCTTGGTCCTCTTCAGGTCCCTCTATTA
TTCCTTCTGATCCTTATGACCGTGCCGTTGCTCGTTTCTGGGCCGCTTAT
GTTGATGAGAAGTTGTTCCCTGCCTTGAAAAGCATTGGTAGTGGAGATGG
AGCGGAAGCTAAGAAAGCTGCGATTGAGCAATTGATTGAAGGCTTGGTTT
TGCTGGAGGATGCTTTTGTTAAGTGTAGCAAAGGGAAGCCTTTCTTTGGT
GGAAGCCAAATTGGGTTTCTTGACATTGCATTTGGTTGCTACTTGGGATG
GGTGAGAGTGACAGAGAAAATGAATGAGGTGAAGCTCCTTGATGAAGTCA
AAACTCCTGGACTGTTCAAATGGGCTGAGAGATTCTGTGCTGATGCCGCT
GTCAAAGATGTTATGCCTGAGACTGATAAGCTTGCAGAGTTTGCTAAGGT
TCTTGCCAAACTCAGAGCTTCTGGCAAATGGAATTAAGAAGCTTAATTCT
GTGCCTGGAGGGGCACNNNNNNNNAANTANNANGAATNANANNNANAAAA
AAAAACCAAAAAANGGAAGGGGNNNNGGCTAAGNNNNAAAGGNNNNCNAA
ACGGANGGGACCNCCGNNAGGACGGGGTTGGTGGATAAAGGAACTTTTAT
TTTTGGCTTGCATATAACATGAGGGCGGGGTGGCGCCACAATGAGATCCA
TTTTTTGCCGCGCGTGCGTTGCCACTGGCATGGTAGAAATGAAGATTCAA
ACTTGGGGGCGGGCGATTATTCCCTCTTGAAAAA
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