EY755911

Overview
NameEY755911
Unique NameEY755911
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length935
Libraries
Library NameType
Sweet orange leaf, field plant Bcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of EY755911 vs. ExPASy Swiss-Prot
Match: CAN9_MOUSE (Calpain-9 OS=Mus musculus GN=Capn9 PE=2 SV=1)

HSP 1 Score: 150.599 bits (379), Expect = 1.206e-35
Identity = 89/230 (38.70%), Postives = 129/230 (56.09%), Query Frame = 3
Query:   24 WVSILEKAYAKLHGSYEALEGGLVQDALVDLTGGAGEEIDMRSAQAQIDLASGRLWSQLLRFKQEGFLLGAG----SPSGSDVHISSSGIVQGHAYSILQVREVDGH----KLVQIRNPWANEVEWNGPWSDSSPEW--TDRMKHKLKHVPQSKDGIFWMSWQDFQIHFRSIYVCRVYPSEMRYSVHGQW----------RGYSAGGCQDYAS--WNQNPQFRLRASGSD 647
            W ++LEKAYAKL+GSYEALEGG   +A+ D TGG  E   +R A           +  L +  + G LLG      + S S+   +S G+++GHAY++  + +V+ H    KL+++RNPW  +VEWNGPWSDSSPEW   D  + K        DG FWM+++DF+IHF  + +C + P  +  S   +W          RG +AGGC+++    W  NPQ +L  +  D
Sbjct:  169 WSALLEKAYAKLNGSYEALEGGSAIEAMEDFTGGVAENFQIREAPED-------FFEILEKALKRGSLLGCSIDTLNASESEAR-TSLGLIKGHAYTVTGLDQVNFHGQRIKLIRVRNPW-GQVEWNGPWSDSSPEWRSVDLEEQKRLGHTALDDGEFWMAFKDFKIHFDKVEICNLTPDALEDSALHRWEVTIHQGSWVRGSTAGGCRNFLDTFWT-NPQIKLSLTERD 388          
BLAST of EY755911 vs. ExPASy Swiss-Prot
Match: CAN_CAEEL (Calpain clp-1 OS=Caenorhabditis elegans GN=clp-1 PE=2 SV=4)

HSP 1 Score: 149.828 bits (377), Expect = 2.057e-35
Identity = 93/236 (39.41%), Postives = 131/236 (55.51%), Query Frame = 3
Query:   24 WVSILEKAYAKLHGSYEALEGGLVQDALVDLTGGAGEEIDMRSAQAQIDLASGRLWSQLLRFKQEGFLLGAGSPSGSDVHIS--SSGIVQGHAYSILQVREVDGHK----LVQIRNPWANEVEWNGPWSDSSPEW---TDRMKHKLKHVPQSKDGIFWMSWQDFQIHFRSIYVCRVYPSEM----------------RYSVH-GQW-RGYSAGGCQDYA-SWNQNPQFRLRASGSD 647
            W ++LEKAYAKL GSYEAL+GG   +AL D+TGG  E ID+++           L   ++R  + G L G    +  +V  +  S+G+V+GHAYSI   R VDG      +++IRNPW NE EWNGPWSD+S EW    D +K  +  +    DG FWMS+ DF  +F  + +C + P  M                  + H G W R  +AGGC++Y  ++  NPQFR++ + SD
Sbjct:  443 WSALLEKAYAKLFGSYEALKGGTTSEALEDMTGGLTEFIDLKNPPR-------NLMQMMMRGFEMGSLFGCSIEADPNVWEAKMSNGLVKGHAYSITGCRIVDGPNGQTCILRIRNPWGNEQEWNGPWSDNSREWRSVPDSVKQDM-GLKFDHDGEFWMSFDDFMRNFEKMEICNLGPDVMDEVYQMTGVKAAGMVWAANTHDGAWVRNQTAGGCRNYINTFANNPQFRVQLTDSD 670          
BLAST of EY755911 vs. ExPASy Swiss-Prot
Match: CANB_DROME (Calpain-B OS=Drosophila melanogaster GN=CalpB PE=1 SV=2)

HSP 1 Score: 145.976 bits (367), Expect = 2.970e-34
Identity = 84/238 (35.29%), Postives = 131/238 (55.04%), Query Frame = 3
Query:   24 WVSILEKAYAKLHGSYEALEGGLVQDALVDLTGGAGEEIDMRSAQAQIDLASGRLWSQLLRFKQEGFLLGAGSPSGSDVHI----SSSGIVQGHAYSILQVREVD--------GHKLVQIRNPWANEVEWNGPWSDSSPEWTDRMKHKLKHVPQS--KDGIFWMSWQDFQIHFRSIYVCRVYP----SEMRYSVHGQWR----------GYSAGGCQDYAS--WNQNPQFRLRASGSD 647
            W ++LEKAYAKLHGSYEAL+GG   +A+ D TGG  E  D++ A          L+S +++  + G ++G       D H+    +  G+++GHAYSI +V  +D           ++++RNPW N+ EW+GPWSDSSPEW    +H  + +  +  +DG FWMS+QDF  HF  + +C + P     + ++S   +W           G +AGGC+++    W+ NPQ+ +     D
Sbjct:  386 WSALLEKAYAKLHGSYEALKGGTTCEAMEDFTGGVTEWYDIKEAPP-------NLFSIMMKAAERGSMMGCSLE--PDPHVLEAETPQGLIRGHAYSITKVCLMDISTPNRQGKLPMIRMRNPWGNDAEWSGPWSDSSPEWRFIPEHTKEEIGLNFDRDGEFWMSFQDFLNHFDRVEICNLSPDSLTEDQQHSSRRKWEMSMFEGEWTSGVTAGGCRNFLETFWH-NPQYIISLEDPD 613          
BLAST of EY755911 vs. ExPASy Swiss-Prot
Match: CAN9_RAT (Calpain-9 (Fragment) OS=Rattus norvegicus GN=Capn9 PE=2 SV=1)

HSP 1 Score: 145.976 bits (367), Expect = 2.970e-34
Identity = 86/230 (37.39%), Postives = 130/230 (56.52%), Query Frame = 3
Query:   24 WVSILEKAYAKLHGSYEALEGGLVQDALVDLTGGAGEEIDMRSAQAQIDLASGRLWSQLLRFKQEGFLLGAG----SPSGSDVHISSSGIVQGHAYSILQVREVDGH----KLVQIRNPWANEVEWNGPWSDSSPEWTDRMKHKLKHVPQS--KDGIFWMSWQDFQIHFRSIYVCRVYPSEM--------RYSVH-GQW-RGYSAGGCQDYAS--WNQNPQFRLRASGSD 647
            W ++LEKAYAKL+GSYEAL+GG   +A+ D TGG  E   +R A           +  L +  + G LLG      + S S+   +  G+++GHAY++  + +V+ H    KL+++RNPW  +VEWNGPWSDSSPEW      + K +  +   DG FWM+++DF+ HF  + +C + P  +          ++H G W RG +AGGC+++    W  NPQ +L  +  D
Sbjct:  151 WSALLEKAYAKLNGSYEALKGGSAIEAMEDFTGGVAENFQIREAPED-------FYEILEKALRRGSLLGCSIDTLNASESEAR-TPLGLIKGHAYTVTGLDQVNFHGQRIKLIRVRNPW-GQVEWNGPWSDSSPEWRSVSLEEQKRLGHTALDDGEFWMAFEDFKTHFDKVEICNLTPDALEDNTLHKWEVTIHQGSWVRGSTAGGCRNFLDTFWT-NPQIKLSLTERD 370          
BLAST of EY755911 vs. ExPASy Swiss-Prot
Match: CANA_DROME (Calpain-A OS=Drosophila melanogaster GN=CalpA PE=1 SV=2)

HSP 1 Score: 145.206 bits (365), Expect = 5.067e-34
Identity = 83/228 (36.40%), Postives = 125/228 (54.82%), Query Frame = 3
Query:   24 WVSILEKAYAKLHGSYEALEGGLVQDALVDLTGGAGEEIDMRSAQAQIDLASGRLWSQLLRFKQEGFLLGAGSPSGSDVHISSS--GIVQGHAYSILQVREVD--------GHKLVQIRNPWANEVEWNGPWSDSSPEWTDRMKHKLKHVPQS--KDGIFWMSWQDFQIHFRSIYVCRVYPSEMRYS-------------VHGQWR-GYSAGGCQDYAS--WNQNPQF 623
            W ++LEKAYAKLHGSYEAL+GG   +A+ D TGG  E  D++        A G L++ L +  +   ++G       +V  + +  G+++GHAYSI +V  +D           ++++RNPW NE EWNGPWSDSSPEW    + +   +  +  +DG FWMS+QDF  HF  + +C + P  +                  G+W  G +AGGC+++    W+ NPQ+
Sbjct:  215 WSALLEKAYAKLHGSYEALKGGSTCEAMEDFTGGVSEWYDLKE-------APGNLFTILQKAAERNSMMGCSIEPDPNVTEAETPQGLIRGHAYSITKVCLIDIVTPNRQGKIPMIRMRNPWGNEAEWNGPWSDSSPEWRYIPEEQKAEIGLTFDRDGEFWMSFQDFLNHFDRVEICNLSPDSLTEDQQNSGKRKWEMSMYEGEWTPGVTAGGCRNFLDTFWH-NPQY 434          
BLAST of EY755911 vs. ExPASy Swiss-Prot
Match: CAN8_MOUSE (Calpain-8 OS=Mus musculus GN=Capn8 PE=1 SV=1)

HSP 1 Score: 144.436 bits (363), Expect = 8.642e-34
Identity = 90/232 (38.79%), Postives = 127/232 (54.74%), Query Frame = 3
Query:   12 GHVLWVSILEKAYAKLHGSYEALEGGLVQDALVDLTGGAGEEIDMRSAQAQIDLASGRLWSQLLRFKQEGFLLGAG---SPSGSDVHISSSGIVQGHAYSILQVREVD----GHKLVQIRNPWANEVEWNGPWSDSSPEWT--DRMKHKLKHVPQSKDGIFWMSWQDFQIHFRSIYVCRVYPSEMRYS---------VHGQW-RGYSAGGCQDY-ASWNQNPQFRLRASGSD 647
            G+  W ++LEKAYAKL+GSYEAL GG   +   D TGG  E  D+R          G L+  + +  ++G LLG     S +      +   +V+GHAYS+  V EVD      KL+++RNPW  EVEW G WSDS+PEW   D  K K +   +++DG FWMS+ DF   F  + +C + P  +             +G+W RG +AGGCQ+Y A++  NPQF++     D
Sbjct:  173 GNEFWSALLEKAYAKLNGSYEALAGGSTIEGFEDFTGGISEFYDLRK-------PPGNLYYTIQKALRKGSLLGCSIDVSNAAEAEATTRQKLVKGHAYSVTGVEEVDFRGLPEKLIRLRNPW-GEVEWTGAWSDSAPEWNYIDPQK-KGELDKRAEDGEFWMSFSDFLKQFSRLEICNLSPDSLSSEEIHKWNLVLFNGRWTRGSTAGGCQNYPATYWTNPQFKIHLDEVD 395          
BLAST of EY755911 vs. ExPASy Swiss-Prot
Match: CAN1_MOUSE (Calpain-1 catalytic subunit OS=Mus musculus GN=Capn1 PE=2 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 1.474e-33
Identity = 87/233 (37.34%), Postives = 133/233 (57.08%), Query Frame = 3
Query:    9 RGHVLWVSILEKAYAKLHGSYEALEGGLVQDALVDLTGGAGEEIDMRSAQAQIDLASGRLWSQLLRFKQEGFLLGAGSPSGSDVH----ISSSGIVQGHAYSILQVREV--DGHK--LVQIRNPWANEVEWNGPWSDSSPEWTDRMKHKLKHV-PQSKDGIFWMSWQDFQIHFRSIYVCRVYPSEMRYSVHGQW----------RGYSAGGCQDY-ASWNQNPQFRLRASGSD 647
            +G+  W ++LEKAYAK++GSYEAL GG   +A  D TGG  E  D++ A +        L+  +L+  + G LLG  S + SD+     I+   +V+GHAYS+   ++V   G +  L+++RNPW  EVEW GPWSDSS EW     ++ + +  + +DG FWMS++DF   F  + +C + P  ++      W          RG +AGGC++Y A++  NPQF++R    D
Sbjct:  182 QGNEFWSALLEKAYAKVNGSYEALSGGCTSEAFEDFTGGVTEWYDLQKAPSD-------LYQIILKALERGSLLGC-SINISDIRDLEAITFKNLVRGHAYSVTGAKQVTYQGQRVNLIRMRNPW-GEVEWKGPWSDSSYEWNKVDPYEREQLRVKMEDGEFWMSFRDFIREFTKLEICNLTPDALKSRTLRNWNTTFYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLEEVD 405          
BLAST of EY755911 vs. ExPASy Swiss-Prot
Match: CAN1_RAT (Calpain-1 catalytic subunit OS=Rattus norvegicus GN=Capn1 PE=1 SV=1)

HSP 1 Score: 143.28 bits (360), Expect = 1.925e-33
Identity = 86/233 (36.91%), Postives = 133/233 (57.08%), Query Frame = 3
Query:    9 RGHVLWVSILEKAYAKLHGSYEALEGGLVQDALVDLTGGAGEEIDMRSAQAQIDLASGRLWSQLLRFKQEGFLLGAGSPSGSDVH----ISSSGIVQGHAYSILQVREV--DGHK--LVQIRNPWANEVEWNGPWSDSSPEWTDRMKHKLKHV-PQSKDGIFWMSWQDFQIHFRSIYVCRVYPSEMRYSVHGQW----------RGYSAGGCQDY-ASWNQNPQFRLRASGSD 647
            +G+  W ++LEKAYAK++GSYEAL GG   +A  D TGG  E  D++ A +        L+  +L+  + G LLG  S + SD+     I+   +V+GHAYS+   ++V   G +  L+++RNPW  EVEW GPWSD+S EW     ++ + +  + +DG FWMS++DF   F  + +C + P  ++      W          RG +AGGC++Y A++  NPQF++R    D
Sbjct:  182 QGNEFWSALLEKAYAKVNGSYEALSGGCTSEAFEDFTGGVTEWYDLQKAPSD-------LYQIILKALERGSLLGC-SINISDIRDLEAITFKNLVRGHAYSVTDAKQVTYQGQRVNLIRMRNPW-GEVEWKGPWSDNSYEWNKVDPYEREQLRVKMEDGEFWMSFRDFIREFTKLEICNLTPDALKSRTLRNWNTTFYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLEEVD 405          
BLAST of EY755911 vs. ExPASy Swiss-Prot
Match: CAN1_PONAB (Calpain-1 catalytic subunit OS=Pongo abelii GN=CAPN1 PE=2 SV=1)

HSP 1 Score: 140.198 bits (352), Expect = 1.630e-32
Identity = 82/231 (35.50%), Postives = 128/231 (55.41%), Query Frame = 3
Query:   12 GHVLWVSILEKAYAKLHGSYEALEGGLVQDALVDLTGGAGEEIDMRSAQAQIDLASGRLWSQLLRFKQEGFLLGAGSPSGSDVH---ISSSGIVQGHAYSILQVREVDGH----KLVQIRNPWANEVEWNGPWSDSSPEWTDRMKHKLKHV-PQSKDGIFWMSWQDFQIHFRSIYVCRVYPSEMRYSVHGQW----------RGYSAGGCQDY-ASWNQNPQFRLRASGSD 647
            G+  W ++LEKAYAK++GSYEAL GG   +   D TGG  E  ++R A +        L+  +L+  + G LLG      S +    I+   +V+GHAYS+   ++V+       L+++RNPW  EVEW G WSDSS EW +   ++   +  + +DG FWMS++DF   F  + +C + P  ++     +W          RG +AGGC++Y A++  NPQF++R   +D
Sbjct:  183 GNEFWSALLEKAYAKVNGSYEALSGGSTSEGFEDFTGGVTEWYELRKAPSD-------LYQIILKALERGSLLGCSIDISSVLDMEAITFKKLVKGHAYSVTGAKQVNYRGQMVSLIRMRNPW-GEVEWTGAWSDSSSEWNNVDPYERDQLRVKMEDGEFWMSFRDFMREFTRLEICNLTPDALKSRTIRKWNTTLYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLDETD 405          
BLAST of EY755911 vs. ExPASy Swiss-Prot
Match: CAN1_HUMAN (Calpain-1 catalytic subunit OS=Homo sapiens GN=CAPN1 PE=1 SV=1)

HSP 1 Score: 140.198 bits (352), Expect = 1.630e-32
Identity = 82/231 (35.50%), Postives = 128/231 (55.41%), Query Frame = 3
Query:   12 GHVLWVSILEKAYAKLHGSYEALEGGLVQDALVDLTGGAGEEIDMRSAQAQIDLASGRLWSQLLRFKQEGFLLGAGSPSGSDVH---ISSSGIVQGHAYSILQVREVDGH----KLVQIRNPWANEVEWNGPWSDSSPEWTDRMKHKLKHV-PQSKDGIFWMSWQDFQIHFRSIYVCRVYPSEMRYSVHGQW----------RGYSAGGCQDY-ASWNQNPQFRLRASGSD 647
            G+  W ++LEKAYAK++GSYEAL GG   +   D TGG  E  ++R A +        L+  +L+  + G LLG      S +    I+   +V+GHAYS+   ++V+       L+++RNPW  EVEW G WSDSS EW +   ++   +  + +DG FWMS++DF   F  + +C + P  ++     +W          RG +AGGC++Y A++  NPQF++R   +D
Sbjct:  183 GNEFWSALLEKAYAKVNGSYEALSGGSTSEGFEDFTGGVTEWYELRKAPSD-------LYQIILKALERGSLLGCSIDISSVLDMEAITFKKLVKGHAYSVTGAKQVNYRGQVVSLIRMRNPW-GEVEWTGAWSDSSSEWNNVDPYERDQLRVKMEDGEFWMSFRDFMREFTRLEICNLTPDALKSRTIRKWNTTLYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLDETD 405          
The following BLAST results are available for this feature:
BLAST of EY755911 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 63
Match NameE-valueIdentityDescription
CAN9_MOUSE1.206e-3538.70Calpain-9 OS=Mus musculus GN=Capn9 PE=2 SV=1[more]
CAN_CAEEL2.057e-3539.41Calpain clp-1 OS=Caenorhabditis elegans GN=clp-1 P... [more]
CANB_DROME2.970e-3435.29Calpain-B OS=Drosophila melanogaster GN=CalpB PE=1... [more]
CAN9_RAT2.970e-3437.39Calpain-9 (Fragment) OS=Rattus norvegicus GN=Capn9... [more]
CANA_DROME5.067e-3436.40Calpain-A OS=Drosophila melanogaster GN=CalpA PE=1... [more]
CAN8_MOUSE8.642e-3438.79Calpain-8 OS=Mus musculus GN=Capn8 PE=1 SV=1[more]
CAN1_MOUSE1.474e-3337.34Calpain-1 catalytic subunit OS=Mus musculus GN=Cap... [more]
CAN1_RAT1.925e-3336.91Calpain-1 catalytic subunit OS=Rattus norvegicus G... [more]
CAN1_PONAB1.630e-3235.50Calpain-1 catalytic subunit OS=Pongo abelii GN=CAP... [more]
CAN1_HUMAN1.630e-3235.50Calpain-1 catalytic subunit OS=Homo sapiens GN=CAP... [more]

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Properties
Property NameValue
Genbank descriptionCS13-C1-001-001-D01-CT.F Sweet orange leaf, field plant B Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY755911 ID=EY755911; Name=EY755911; organism=Citrus sinensis; type=EST; length=935bp
CACGCGTCCGGGGTCATGTACTCTGGGTCTCTATACTGGAGAAGGCATAT
GCCAAATTGCATGGTTCTTATGAGGCACTGGAAGGAGGACTTGTTCAGGA
TGCTCTTGTGGACCTCACTGGGGGTGCTGGTGAGGAGATTGACATGAGAA
GTGCCCAGGCTCAGATTGATCTTGCAAGTGGTAGATTATGGTCCCAATTG
TTGCGCTTTAAACAAGAAGGATTCCTACTTGGTGCTGGGAGTCCATCAGG
TTCTGATGTGCATATTTCTTCCAGTGGCATAGTGCAAGGCCATGCTTATT
CAATTCTACAGGTGAGGGAGGTGGATGGCCACAAGCTTGTTCAAATTCGA
AATCCATGGGCTAATGAAGTTGAATGGAATGGCCCCTGGTCTGATTCGTC
ACCTGAATGGACTGACAGGATGAAACACAAGCTAAAACATGTTCCGCAGT
CAAAAGATGGTATATTCTGGATGTCCTGGCAAGATTTTCAGATTCACTTT
CGATCAATATATGTCTGCCGTGTTTATCCGTCCGAGATGCGCTATTCTGT
GCACGGCCAATGGCGTGGTTACAGTGCAGGTGGTTGCCAAGATTATGCTT
CATGGAATCAGAATCCTCAGTTTCGATTGAGGGCCTCTGGATCGGATGCA
TCATTTCCTATTCATGTTTTCATTACCTTGACGCAAGGTGTGAGTTTCTC
AAGAACCAGTGCTGGTTTTAAAAATATCCAATCAGTCACGATTCTATGAT
GTTCTATATTGGAATGAGGATACTGAAAACTCGTGGNCGCCGTGCTGCTA
CCAAACATTACTACATGAATCAGTTGGTGGGACCAATATGGAAATTCCCG
GGAAATTTCTTGGAGATTGGTCTGGATCCTGACCCGAGGGCTCCCAATAA
GGCCTCACCCTTACCCTGGGGAAGAGCCCATTTGT
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