Genetic analysis of apomixis in Citrus and Poncirus by molecular markers

Publication Overview
TitleGenetic analysis of apomixis in Citrus and Poncirus by molecular markers
AuthorsGarcía R, Asíns MJ, Forner J, Carbonell EA
TypeJournal Article
Journal NameTheoretical and Applied Genetics
Volume99
Issue3-4
Year1999
Page(s)511-518
CitationGarcía R, Asíns MJ, Forner J, Carbonell EA. Genetic analysis of apomixis in Citrus and Poncirus by molecular markers. Theoretical and applied genetics TAG. 1999; 99(3-4):511-518.

Abstract

Propagation of citrus rootstocks depends upon the production of clonal plants from nucellar seedlings. This makes apomixis one of the host important traits in breeding programs for citrus rootstocks. The genetic control of apomixis was studied in a 50-tree progeny derived from the cross C. volkameriana×P. trifoliata using 69 molecular markers and bulked segregant analysis. The proportion of nucellar seedlings was estimated by isoenzymatic analysis of 25 seedlings per tree for 2 consecutive years. The type of embryony (polyembryonic versus monoembryonic seeds) was also determined for fruit-yielding trees. Separate genetic maps for each parental species were developed. The integration and comparison of these maps could be accomplished using common multiallelic segregant loci. Differences in gene synteny between the two species-specific genetic maps were shown. Important distortions in the segregation of markers at several genomic regions, some of them also involving differences in the C-methylation pattern, have been observed, especially for the pollen parent. Analysis of quantitative trait loci (QTLs) revealed the presence of six genomic positions (two in P. trifoliata and four in C. volkameriana) contributing individually up to 24% of the total variation for apomixis. Within the same species, QTLs with positive and negative allele effects were present, even in the same linkage group. One of the markers associated to apomixis (Apo2) is also associated to embryony type. Therefore, the genetic control of apomictic reproduction found in citrus (nucellar embryony) is quite complex compared to what has been reported for gametophytic apomixis. Molecular markers linked to QTLs governing apomixis will be useful to assist selection of future apomictic rootstocks for citrus varieties.
Featuremaps
This publication contains information about 3 maps:
Map Name
Citrus-Volkamer/Rubidoux-Volkamer-F1-1996
Citrus-Volkamer/Rubidioux-Rubidoux-F1-1999
Citrus-Volkamer/Rubidoux-F1-1996
Stocks
This publication contains information about 5 stocks:
Stock NameUniquenameType
VolkamerVolkamercultivar
RubidouxRubidouxcultivar
Citrus-Volkamer/Rubidoux-F1Citrus-Volkamer/Rubidoux-F1population
Citrus-Volkamer/Rubidoux-Volkamer-F1Citrus-Volkamer/Rubidoux-Volkamer-F1population
Citrus-Volkamer/Rubidioux-Rubidoux-F1Citrus-Volkamer/Rubidioux-Rubidoux-F1population
Properties
Additional details for this publication include:
Property NameValue
Language Abbreng
Publication Date1999
Publication Model[electronic resource].
Publication TypeJournal Article
URLhttp://dx.doi.org/10.1007/s001220051264
KeywordsCitrus, Poncirus, alleles, apomixis, chromosome mapping, gametophytes, genetic markers, hosts, linkage groups, loci, parents, pollen, progeny, quantitative analysis, quantitative trait loci, rootstocks, seedlings, seeds, segregation distortion, trees
Cross References
This publication is also available in the following databases:
DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:4740503