EY729805

Overview
NameEY729805
Unique NameEY729805
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length790
Libraries
Library NameType
Sweet orange fruit, development stadium (5 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY729805 vs. ExPASy Swiss-Prot
Match: ASTE_PSEFS (Succinylglutamate desuccinylase OS=Pseudomonas fluorescens (strain SBW25) GN=astE PE=3 SV=1)

HSP 1 Score: 177.948 bits (450), Expect = 5.427e-44
Identity = 96/133 (72.18%), Postives = 106/133 (79.70%), Query Frame = 2 
Query:  380 EPAQKIQLTVDGVQLRWLSEGALEVRXPEARDNGSDVLLSSGIHGNETAPIELGRRLAASIARGENQPRTRILFWFGKPEGMRPGERYL*LDVNRLFNGRHEQSIGPKAF-APPTPTVGRTFSSC-GAYRLTY 772
            EPAQKIQLTVDGVQ+RWLSEGALEVR PEARDNGSD+LLSSGIHGNETAPIEL  RL   IARGE +PR+R+LF FG  E MR GERYL LDVNRLFNGRHE++IGP+A  A     + R+F S  G  RL Y
Sbjct:   16 EPAQKIQLTVDGVQMRWLSEGALEVRPPEARDNGSDLLLSSGIHGNETAPIELLDRLLHGIARGEIKPRSRVLFLFGNTEAMRRGERYLELDVNRLFNGRHEKNIGPEAMRAAELEQLARSFFSLPGRSRLHY 148
[Close]
BLAST of EY729805 vs. ExPASy Swiss-Prot
Match: ASTE_PSEPF (Succinylglutamate desuccinylase OS=Pseudomonas fluorescens (strain Pf0-1) GN=astE PE=3 SV=1)

HSP 1 Score: 159.073 bits (401), Expect = 2.609e-38
Identity = 79/109 (72.48%), Postives = 91/109 (83.49%), Query Frame = 2 
Query:  380 EPAQKIQLTVDGVQLRWLSEGALEVRXPEARDNGSDVLLSSGIHGNETAPIELGRRLAASIARGENQPRTRILFWFGKPEGMRPGERYL*LDVNRLFNGRHEQSIGPKA 706
            EPA+K QLTV+GV++RWLSEGALEVR PEARDNG D+LLS+GIHGNETAPIEL  RL   IARG+ +PR RILF FG PE +R GER++  DVNRLFNGRHEQS G +A
Sbjct:   16 EPAEKTQLTVEGVRMRWLSEGALEVRPPEARDNGLDLLLSAGIHGNETAPIELLDRLLHDIARGDLKPRARILFLFGNPEAIRKGERFVEQDVNRLFNGRHEQSSGSEA 124
[Close]
BLAST of EY729805 vs. ExPASy Swiss-Prot
Match: ASTE_PSEF5 (Succinylglutamate desuccinylase OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=astE PE=3 SV=1)

HSP 1 Score: 158.688 bits (400), Expect = 3.407e-38
Identity = 80/109 (73.39%), Postives = 90/109 (82.57%), Query Frame = 2 
Query:  380 EPAQKIQLTVDGVQLRWLSEGALEVRXPEARDNGSDVLLSSGIHGNETAPIELGRRLAASIARGENQPRTRILFWFGKPEGMRPGERYL*LDVNRLFNGRHEQSIGPKA 706
            EPA+K QLTVDGV++RWLSEGALEVR PEARDNG D+LLS+GIHGNETAPIEL  RL   IARG+ +PR RILF FG PE MR GER++  DVNRLFNGRHE S G +A
Sbjct:   16 EPAEKTQLTVDGVRMRWLSEGALEVRPPEARDNGLDLLLSAGIHGNETAPIELLDRLLHDIARGDLKPRARILFLFGNPEAMRRGERFVEQDVNRLFNGRHESSSGFEA 124
[Close]
BLAST of EY729805 vs. ExPASy Swiss-Prot
Match: ASTE_PSESM (Succinylglutamate desuccinylase OS=Pseudomonas syringae pv. tomato GN=astE PE=3 SV=1)

HSP 1 Score: 154.836 bits (390), Expect = 4.920e-37
Identity = 77/109 (70.64%), Postives = 90/109 (82.57%), Query Frame = 2 
Query:  380 EPAQKIQLTVDGVQLRWLSEGALEVRXPEARDNGSDVLLSSGIHGNETAPIELGRRLAASIARGENQPRTRILFWFGKPEGMRPGERYL*LDVNRLFNGRHEQSIGPKA 706
            EPA+K QLTV+GV++RWL+EGALEVR P+ARDNG+D+LLS+GIHGNETAPIEL   L  SIARG+ +PR RILF FG P  MR G RY+  DVNRLFNGRHEQS G +A
Sbjct:   16 EPAEKTQLTVEGVRMRWLAEGALEVRPPQARDNGTDLLLSAGIHGNETAPIELLDELIRSIARGDLKPRARILFLFGNPAAMRLGARYVEQDVNRLFNGRHEQSGGAEA 124
[Close]
BLAST of EY729805 vs. ExPASy Swiss-Prot
Match: ASTE_PSE14 (Succinylglutamate desuccinylase OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=astE PE=3 SV=1)

HSP 1 Score: 152.14 bits (383), Expect = 3.189e-36
Identity = 74/109 (67.89%), Postives = 89/109 (81.65%), Query Frame = 2 
Query:  380 EPAQKIQLTVDGVQLRWLSEGALEVRXPEARDNGSDVLLSSGIHGNETAPIELGRRLAASIARGENQPRTRILFWFGKPEGMRPGERYL*LDVNRLFNGRHEQSIGPKA 706
            EPA+K QLTV+GV++RW+ EGALEVR P+ RDNG+D+LLS+GIHGNETAPIEL   L  SIARG+ +PR RILF FG P+ MR G R++  DVNRLFNGRHEQS G +A
Sbjct:   16 EPAEKTQLTVEGVRMRWMGEGALEVRPPQVRDNGTDLLLSAGIHGNETAPIELLDELIRSIARGDLKPRARILFLFGNPDAMRRGTRFVEQDVNRLFNGRHEQSGGAEA 124
[Close]
BLAST of EY729805 vs. ExPASy Swiss-Prot
Match: ASTE_PSEU2 (Succinylglutamate desuccinylase OS=Pseudomonas syringae pv. syringae (strain B728a) GN=astE PE=3 SV=1)

HSP 1 Score: 151.754 bits (382), Expect = 4.165e-36
Identity = 75/109 (68.81%), Postives = 89/109 (81.65%), Query Frame = 2 
Query:  380 EPAQKIQLTVDGVQLRWLSEGALEVRXPEARDNGSDVLLSSGIHGNETAPIELGRRLAASIARGENQPRTRILFWFGKPEGMRPGERYL*LDVNRLFNGRHEQSIGPKA 706
            EPA+K QLTV+GV++RW+ EGALEVR PEARDNG+D+LLS+GIHGNETAPIEL   L  SIARG+ +PR RILF FG P+ MR G R++  DVNRLFNGRH QS G +A
Sbjct:   16 EPAEKTQLTVEGVRMRWMGEGALEVRPPEARDNGTDLLLSAGIHGNETAPIELLDELIRSIARGDLKPRARILFLFGNPDAMRRGARFVEQDVNRLFNGRHLQSGGAEA 124
[Close]
BLAST of EY729805 vs. ExPASy Swiss-Prot
Match: ASTE_PSEMY (Succinylglutamate desuccinylase OS=Pseudomonas mendocina (strain ymp) GN=astE PE=3 SV=1)

HSP 1 Score: 146.747 bits (369), Expect = 1.340e-34
Identity = 74/109 (67.89%), Postives = 83/109 (76.15%), Query Frame = 2 
Query:  380 EPAQKIQLTVDGVQLRWLSEGALEVRXPEARDNGSDVLLSSGIHGNETAPIELGRRLAASIARGENQPRTRILFWFGKPEGMRPGERYL*LDVNRLFNGRHEQSIGPKA 706
            EP+ KIQLT DG +LRWL EGALE+  P ARDNG D+LLS+GIHGNETAPIEL  RL   IAR E  P  RILF FG PE MR GER++  D+NRLFNGRHEQS G +A
Sbjct:   16 EPSAKIQLTPDGTRLRWLDEGALEITPPAARDNGLDLLLSAGIHGNETAPIELLDRLLRGIARNELHPAARILFLFGNPEAMRRGERFVEQDINRLFNGRHEQSSGFEA 124
[Close]
BLAST of EY729805 vs. ExPASy Swiss-Prot
Match: ASTE_PSEAE (Succinylglutamate desuccinylase OS=Pseudomonas aeruginosa GN=astE PE=3 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 9.602e-33
Identity = 69/110 (62.73%), Postives = 82/110 (74.55%), Query Frame = 2 
Query:  380 EPAQKIQLTVDGVQLRWLSEGALEVRXPEARDNGSDVLLSSGIHGNETAPIELGRRLAASIARGENQPRTRILFWFGKPEGMRPGERYL*LDVNRLFNGRHEQSIGPKAF 709
            EP +KIQLT DG +L WL+EGALEV    ARDNG D+LLS+GIHGNETAPIEL  RL   +A G  +P  R+LF FG PE +R GERY+  D+NRLFNGRHE+  G +AF
Sbjct:   16 EPTEKIQLTADGTRLHWLAEGALEVTPIGARDNGVDLLLSAGIHGNETAPIELLERLIRKVAAGTLKPAARVLFLFGNPEAIRRGERYVEQDMNRLFNGRHEEGSGNEAF 125
[Close]
BLAST of EY729805 vs. ExPASy Swiss-Prot
Match: ASTE_PSEAB (Succinylglutamate desuccinylase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=astE PE=3 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 9.602e-33
Identity = 69/110 (62.73%), Postives = 82/110 (74.55%), Query Frame = 2 
Query:  380 EPAQKIQLTVDGVQLRWLSEGALEVRXPEARDNGSDVLLSSGIHGNETAPIELGRRLAASIARGENQPRTRILFWFGKPEGMRPGERYL*LDVNRLFNGRHEQSIGPKAF 709
            EP +KIQLT DG +L WL+EGALEV    ARDNG D+LLS+GIHGNETAPIEL  RL   +A G  +P  R+LF FG PE +R GERY+  D+NRLFNGRHE+  G +AF
Sbjct:   16 EPTEKIQLTADGTRLHWLAEGALEVTPIGARDNGVDLLLSAGIHGNETAPIELLERLIRKVAAGTLKPAARVLFLFGNPEAIRRGERYVEQDMNRLFNGRHEEGSGNEAF 125
[Close]
BLAST of EY729805 vs. ExPASy Swiss-Prot
Match: ASTE_PSEA8 (Succinylglutamate desuccinylase OS=Pseudomonas aeruginosa (strain LESB58) GN=astE PE=3 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 9.602e-33
Identity = 69/110 (62.73%), Postives = 82/110 (74.55%), Query Frame = 2 
Query:  380 EPAQKIQLTVDGVQLRWLSEGALEVRXPEARDNGSDVLLSSGIHGNETAPIELGRRLAASIARGENQPRTRILFWFGKPEGMRPGERYL*LDVNRLFNGRHEQSIGPKAF 709
            EP +KIQLT DG +L WL+EGALEV    ARDNG D+LLS+GIHGNETAPIEL  RL   +A G  +P  R+LF FG PE +R GERY+  D+NRLFNGRHE+  G +AF
Sbjct:   16 EPTEKIQLTADGTRLHWLAEGALEVTPIGARDNGVDLLLSAGIHGNETAPIELLERLIRKVAAGTLKPAARVLFLFGNPEAIRRGERYVEQDMNRLFNGRHEEGSGNEAF 125
The following BLAST results are available for this feature:
BLAST of EY729805 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 78
Match NameE-valueIdentityDescription
ASTE_PSEFS5.427e-4472.18Succinylglutamate desuccinylase OS=Pseudomonas flu... [more]
ASTE_PSEPF2.609e-3872.48Succinylglutamate desuccinylase OS=Pseudomonas flu... [more]
ASTE_PSEF53.407e-3873.39Succinylglutamate desuccinylase OS=Pseudomonas flu... [more]
ASTE_PSESM4.920e-3770.64Succinylglutamate desuccinylase OS=Pseudomonas syr... [more]
ASTE_PSE143.189e-3667.89Succinylglutamate desuccinylase OS=Pseudomonas syr... [more]
ASTE_PSEU24.165e-3668.81Succinylglutamate desuccinylase OS=Pseudomonas syr... [more]
ASTE_PSEMY1.340e-3467.89Succinylglutamate desuccinylase OS=Pseudomonas men... [more]
ASTE_PSEAE9.602e-3362.73Succinylglutamate desuccinylase OS=Pseudomonas aer... [more]
ASTE_PSEAB9.602e-3362.73Succinylglutamate desuccinylase OS=Pseudomonas aer... [more]
ASTE_PSEA89.602e-3362.73Succinylglutamate desuccinylase OS=Pseudomonas aer... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-704-021-E12-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY729805 ID=EY729805; Name=EY729805; organism=Citrus sinensis; type=EST; length=790bp
ATCAATTAACGCCGCACGGCTGAGAACTATTTATGACCACCGACACCCTG
AAACTGATCCTTGAAGACACCGACGGCACCCAGCTGGAAACGTCCTGCAC
CCGCGTCGCCGTGGTGTGGCAGGGCAAGGAGATCTGGATCCAGCACGACG
GCCGTGGCCAACTGTTGATCGGCGTTGACGTGGAAGAGGGCGACGCGGAA
TACGCCAACCTGCTGATGCGCCCATTGGCCACCAACCTGATGAGCCTGCA
ACTGGAAATGGAACCGGCCGACGTTGAAGGTGATGATGACGATCACGTCC
ACGGTCCTGGCTGCAACCACTAAGGAAGCTGCTTATGCTCGCCCTCGGCA
AACTGCTTGAACTGACCCTCGCCGGTCGCGAACCGGCGCAAAAAATTCAA
CTGACTGTCGATGGCGTGCAACTGCGCTGGCTCAGTGAGGGCGCGCTGGA
AGTGCGGNCTCCTGAGGCGCGGGACAACGGCAGCGACGTGCTGCTGTCGT
CCGGCATCCATGGCAACGAAACCGCGCCGATCGAATTGGGCCGACGCCTT
GCTGCAAGCATTGCCCGTGGGGAGAATCAGCCCCGCACCCGTATTCTGTT
CTGGTTCGGTAAACCCGAGGGCATGCGCCCCGGCGAGCGTTACTTGTAAC
TGGACGTCAACCGGCTGTTCAACGGGCGTCATGAACAAAGCATTGGCCCG
AAAGCATTCGCGCCGCCGACCCCAACAGTGGGCCGTACTTTTTCATCGTG
CGGGGCGTATCGTTTGACTTACACCTGAAACCGCATCGTG
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