EY728712

Overview
NameEY728712
Unique NameEY728712
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length861
Libraries
Library NameType
Sweet orange fruit, development stadium (5 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of EY728712 vs. ExPASy Swiss-Prot
Match: THI4_CITSI (Thiazole biosynthetic enzyme, chloroplastic OS=Citrus sinensis GN=THI1 PE=2 SV=1)

HSP 1 Score: 174.096 bits (440), Expect = 9.016e-43
Identity = 89/109 (81.65%), Postives = 95/109 (87.16%), Query Frame = 1
Query:  163 NIQIAIIEQSVSPGGGAWLSGQLFSAMVVRKPAHIFLDELGIDYDEQDSYVVIKHAALFTSTIMSELLARPNVKLFSAVAVEDLIXXXXXXXXXXSNWALVVMSLDTQA 489
            NIQIAIIEQSVSPGGGAWL GQLFSAMVVRKPAHIFLDELGIDYDEQD+YVVIKHAALFTSTIMS+LLARPNVKLF+AVA EDLI          +NWALV M+ DTQ+
Sbjct:  114 NIQIAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHIFLDELGIDYDEQDNYVVIKHAALFTSTIMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQS 222          
BLAST of EY728712 vs. ExPASy Swiss-Prot
Match: THI4_ALNGL (Thiazole biosynthetic enzyme, chloroplastic OS=Alnus glutinosa GN=THI4 PE=2 SV=1)

HSP 1 Score: 167.548 bits (423), Expect = 8.439e-41
Identity = 83/109 (76.15%), Postives = 94/109 (86.24%), Query Frame = 1
Query:  163 NIQIAIIEQSVSPGGGAWLSGQLFSAMVVRKPAHIFLDELGIDYDEQDSYVVIKHAALFTSTIMSELLARPNVKLFSAVAVEDLIXXXXXXXXXXSNWALVVMSLDTQA 489
            ++Q+AIIEQSVSPGGGAWL GQLFS MVVRKPAH+FLDELGI+YDEQD+YVVIKHAALFTSTIMS+LLARPNVKLF+AVA EDLI          +NWALV M+ DTQ+
Sbjct:  110 SVQVAIIEQSVSPGGGAWLGGQLFSGMVVRKPAHLFLDELGIEYDEQDNYVVIKHAALFTSTIMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQS 218          
BLAST of EY728712 vs. ExPASy Swiss-Prot
Match: THI4_ARATH (Thiazole biosynthetic enzyme, chloroplastic OS=Arabidopsis thaliana GN=THI1 PE=1 SV=1)

HSP 1 Score: 163.696 bits (413), Expect = 1.219e-39
Identity = 79/109 (72.48%), Postives = 92/109 (84.40%), Query Frame = 1
Query:  163 NIQIAIIEQSVSPGGGAWLSGQLFSAMVVRKPAHIFLDELGIDYDEQDSYVVIKHAALFTSTIMSELLARPNVKLFSAVAVEDLIXXXXXXXXXXSNWALVVMSLDTQA 489
            N+Q+AIIEQSVSPGGGAWL GQLFSAM+VRKPAH+FLDE+G+ YDEQD+YVV+KHAALFTSTIMS+LLARPNVKLF+AVA EDLI          +NWALV  +  TQ+
Sbjct:  107 NVQVAIIEQSVSPGGGAWLGGQLFSAMIVRKPAHLFLDEIGVAYDEQDTYVVVKHAALFTSTIMSKLLARPNVKLFNAVAAEDLIVKGNRVGGVVTNWALVAQNHHTQS 215          
BLAST of EY728712 vs. ExPASy Swiss-Prot
Match: THI41_MAIZE (Thiazole biosynthetic enzyme 1-1, chloroplastic OS=Zea mays GN=THI1-1 PE=2 SV=1)

HSP 1 Score: 160.229 bits (404), Expect = 1.347e-38
Identity = 80/108 (74.07%), Postives = 90/108 (83.33%), Query Frame = 1
Query:  166 IQIAIIEQSVSPGGGAWLSGQLFSAMVVRKPAHIFLDELGIDYDEQDSYVVIKHAALFTSTIMSELLARPNVKLFSAVAVEDLIXXXXXXXXXXSNWALVVMSLDTQA 489
            + IAI+EQSVSPGGGAWL GQLFSAMVVRKPAH+FLDELG+ YDE + YVVIKHAALFTST+MS LLARPNVKLF+AVAVEDLI          +NWALV M+ DTQ+
Sbjct:  114 VSIAIVEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDELGVAYDEAEDYVVIKHAALFTSTVMSLLLARPNVKLFNAVAVEDLIVRGGRVGGVVTNWALVSMNHDTQS 221          
BLAST of EY728712 vs. ExPASy Swiss-Prot
Match: THI42_MAIZE (Thiazole biosynthetic enzyme 1-2, chloroplastic OS=Zea mays GN=THI1-2 PE=2 SV=1)

HSP 1 Score: 159.844 bits (403), Expect = 1.760e-38
Identity = 77/108 (71.30%), Postives = 90/108 (83.33%), Query Frame = 1
Query:  166 IQIAIIEQSVSPGGGAWLSGQLFSAMVVRKPAHIFLDELGIDYDEQDSYVVIKHAALFTSTIMSELLARPNVKLFSAVAVEDLIXXXXXXXXXXSNWALVVMSLDTQA 489
            + +AI+EQSVSPGGGAWL GQLFSAMVVR+PAH+FLDELG+ YDE + YVV+KHAALFTST+MS LLARPNVKLF+AVAVEDLI          +NWALV M+ DTQ+
Sbjct:  111 VSVAIVEQSVSPGGGAWLGGQLFSAMVVRRPAHLFLDELGVGYDEAEDYVVVKHAALFTSTVMSRLLARPNVKLFNAVAVEDLIVRRGRVGGVVTNWALVSMNHDTQS 218          
BLAST of EY728712 vs. ExPASy Swiss-Prot
Match: THI4_FUSOX (Thiazole biosynthetic enzyme, mitochondrial OS=Fusarium oxysporum GN=STI35 PE=2 SV=2)

HSP 1 Score: 129.798 bits (325), Expect = 1.950e-29
Identity = 65/117 (55.56%), Postives = 86/117 (73.50%), Query Frame = 1
Query:  151 QQKTNIQIAIIEQSVSPGGGAWLSGQLFSAMVVRKPAHIFLDELGIDYDEQDSYVVIKHAALFTSTIMSELLARPNVKLFSAVAVEDLI----XXXXXXXXXXSNWALVVMSLDTQA 489
            +++ +++IAIIE SVSPGGGAWL GQLFSAM++RKPA  FL E+G+ Y+++ +YVV+KHAALFTSTIMS++L  PN+KLF+A  VEDLI              +NW LV M  D Q+
Sbjct:   92 KKRPDLKIAIIEASVSPGGGAWLGGQLFSAMIMRKPADAFLREVGVPYEDEGNYVVVKHAALFTSTIMSKVLQMPNIKLFNATCVEDLITRPSEEGVRIAGVVTNWTLVSMHHDDQS 208          
BLAST of EY728712 vs. ExPASy Swiss-Prot
Match: THI4_FUSSH (Thiazole biosynthetic enzyme, mitochondrial OS=Fusarium solani subsp. phaseoli GN=STI35 PE=2 SV=1)

HSP 1 Score: 129.413 bits (324), Expect = 2.547e-29
Identity = 67/117 (57.26%), Postives = 85/117 (72.65%), Query Frame = 1
Query:  151 QQKTNIQIAIIEQSVSPGGGAWLSGQLFSAMVVRKPAHIFLDELGIDYDEQDSYVVIKHAALFTSTIMSELLARPNVKLFSAVAVEDLIXXXXXXXXXXS----NWALVVMSLDTQA 489
            +++ +++IAIIE SVSPGGGAWL GQLFSAMV+RKPA  FL E+G+ Y+++ +YVV+KHAALFTSTIMS++L  PN KLF+A  VEDLI          S    NW LV M  D Q+
Sbjct:   96 KKRPDLKIAIIEASVSPGGGAWLGGQLFSAMVMRKPADAFLREVGVPYEDEGNYVVVKHAALFTSTIMSKVLQLPNCKLFNATCVEDLITRPSKEGVRISGVVTNWTLVSMHHDDQS 212          
BLAST of EY728712 vs. ExPASy Swiss-Prot
Match: THI2_SCHPO (Thiazole biosynthetic enzyme, mitochondrial OS=Schizosaccharomyces pombe GN=thi2 PE=2 SV=2)

HSP 1 Score: 129.413 bits (324), Expect = 2.547e-29
Identity = 65/117 (55.56%), Postives = 86/117 (73.50%), Query Frame = 1
Query:  154 QKTNIQIAIIEQSVSPGGGAWLSGQLFSAMVVRKPAHIFLDELGIDYDEQDSYVVIKHAALFTSTIMSELLARPNVKLFSAVAVEDLI-----XXXXXXXXXXSNWALVVMSLDTQA 489
            ++ +++IAIIE SV+PGGGAWL GQLFSAMVVRKPA +FL+E+G+ Y+++  YVV+KHAALFTST+M+  LA PNVKLF+A AVEDLI               +NW LV ++   Q+
Sbjct:   98 RRPDLKIAIIEASVAPGGGAWLGGQLFSAMVVRKPADLFLNEIGVPYEDEGDYVVVKHAALFTSTVMARTLALPNVKLFNATAVEDLIVKEGKDGKQRIAGVVTNWTLVSLNHGLQS 214          
BLAST of EY728712 vs. ExPASy Swiss-Prot
Match: THI4_ASPOR (Thiazole biosynthetic enzyme, mitochondrial OS=Aspergillus oryzae GN=thiA PE=3 SV=2)

HSP 1 Score: 125.176 bits (313), Expect = 4.803e-28
Identity = 65/118 (55.08%), Postives = 86/118 (72.88%), Query Frame = 1
Query:  148 AQQKTNIQIAIIEQSVSPGGGAWLSGQLFSAMVVRKPAHIFLDELGIDYDE--QDSYVVIKHAALFTSTIMSELLARPNVKLFSAVAVEDLI-----XXXXXXXXXXSNWALVVMSLD 480
            A+ + +++IAI+E SVSPGGGAWL GQLFSAMV+R+PA +FL+ELG+ Y+E    +YVV+KHA+LFTST+MS++L+ PNVKLF+A AVEDLI                NW LV +  D
Sbjct:   95 AKARPDLKIAIVEASVSPGGGAWLGGQLFSAMVMRRPAEVFLNELGVPYEEDANPNYVVVKHASLFTSTLMSKVLSFPNVKLFNATAVEDLITRPTENGNPQIAGVVVNWTLVTLHHD 212          
BLAST of EY728712 vs. ExPASy Swiss-Prot
Match: THI4_YEAST (Thiazole biosynthetic enzyme, mitochondrial OS=Saccharomyces cerevisiae GN=THI4 PE=1 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 6.273e-28
Identity = 61/119 (51.26%), Postives = 83/119 (69.75%), Query Frame = 1
Query:  148 AQQKTNIQIAIIEQSVSPGGGAWLSGQLFSAMVVRKPAHIFLDELGIDYDEQDSYVVIKHAALFTSTIMSELLARPNVKLFSAVAVEDLI------XXXXXXXXXXSNWALVVMSLDTQ 486
            A+ + ++++ IIE SV+PGGG+WL GQLFSAMV+RKPAH+FL EL I Y+++  YVV+KHAALF ST++S++L  PNVKLF+A  VEDL+                +NW LV  +  TQ
Sbjct:   85 AKNRPDLKVCIIESSVAPGGGSWLGGQLFSAMVMRKPAHLFLQELEIPYEDEGDYVVVKHAALFISTVLSKVLQLPNVKLFNATCVEDLVTRPPTEKGEVTVAGVVTNWTLVTQAHGTQ 203          
The following BLAST results are available for this feature:
BLAST of EY728712 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 11
Match NameE-valueIdentityDescription
THI4_CITSI9.016e-4381.65Thiazole biosynthetic enzyme, chloroplastic OS=Cit... [more]
THI4_ALNGL8.439e-4176.15Thiazole biosynthetic enzyme, chloroplastic OS=Aln... [more]
THI4_ARATH1.219e-3972.48Thiazole biosynthetic enzyme, chloroplastic OS=Ara... [more]
THI41_MAIZE1.347e-3874.07Thiazole biosynthetic enzyme 1-1, chloroplastic OS... [more]
THI42_MAIZE1.760e-3871.30Thiazole biosynthetic enzyme 1-2, chloroplastic OS... [more]
THI4_FUSOX1.950e-2955.56Thiazole biosynthetic enzyme, mitochondrial OS=Fus... [more]
THI4_FUSSH2.547e-2957.26Thiazole biosynthetic enzyme, mitochondrial OS=Fus... [more]
THI2_SCHPO2.547e-2955.56Thiazole biosynthetic enzyme, mitochondrial OS=Sch... [more]
THI4_ASPOR4.803e-2855.08Thiazole biosynthetic enzyme, mitochondrial OS=Asp... [more]
THI4_YEAST6.273e-2851.26Thiazole biosynthetic enzyme, mitochondrial OS=Sac... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-704-007-F02-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY728712 ID=EY728712; Name=EY728712; organism=Citrus sinensis; type=EST; length=861bp
GCGCACGTTGTCAGGTACCGGTGCGGAATTCCTAGGTCGGACCACGCGTC
CGATGACGCGCAGGTAGATGACGGACATGATCACTTATGCTGACACTGAT
GTGGTAGTGGTTGGTGCTGGCTCTGCTGGCCTCTCTTGTGCTTATGAGCT
CAGCAAAAAACCAACATTCAGATTGCTATTATCGAACAGTCTGTTAGCCC
CGGCGGTGGCGCGTGGCTCAGTGGACAGCTCTTCTCTGCCATGGTTGTGC
GCAAACCAGCCCACATCTTCCTTGACGAGCTTGGCATCGACTACGATGAG
CAAGACAGCTATGTTGTGATCAAGCATGCAGCGCTGTTCACCTCCACTAT
AATGAGCGAGCTCCTGGCCCGGCCCAACGTCAAGCTCTTTAGTGCAGTGG
CAGTCGAGGACTTGATAGTCAGAGGCGGCAGAGTTGGTGGTGTGGTGAGC
AACTGGGCGTTGGTGGTCATGAGTCTCGATACACAGGCTGGCGTGGATGC
CAATGTAGTGGAGGCCAAGGTGGTGGTGAGCGCTGGGGGGCTGATGGGGC
TGTTGGGGCCGCGGACTGATGACGTGGGGGAGGTTGGGGTGAGGGGGGGG
GTGCGGGGGAGGATGGCTGGGGTTGTGGTGTGCCAAAAGCCGCTTTGGTG
GCGACGCGCGAGTGGAGCGGGGGAGCGGTAGGGGTGTGAGGGTGCTGCGC
TCGGGGTGGCTACATGGGAGTCGCCGGGTGGTGAGCGCATCGGGTGTCTG
TTCGTAGGGTGGGCTGGCCGGGTGGTCGGTGGATGCTTGGGGTGGTGGGC
GGCGGATGGGCTGGTGGGCCCGGCATGGCGGCGCGTGGGCGGGGGCGGAG
GGGATATGCGC
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