EY728479 | Citrus Genome Database

Overview


Name	EY728479
Unique Name	EY728479
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	803

Libraries


Library Name	Type
Sweet orange fruit, development stadium (5 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
EY728479_ssr299	microsatellite	EY728479_ssr299:299..313.	BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig760	contig	Csv1_Contig760:77..878.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of EY728479 vs. ExPASy Swiss-Prot
Match: DNJH_CUCSA (DnaJ protein homolog OS=Cucumis sativus GN=DNAJ1 PE=2 SV=1)

HSP 1 Score: 268.085 bits (684), Expect = 4.095e-71
Identity = 151/231 (65.37%), Postives = 158/231 (68.40%), Query Frame = 2

Query:   53 MFGRAPKKSDNTKYYEILGVSKNASQDDLKKAYRKAAIKNHPDKGGDPEKFKELAQAYEVLSDPQKREIYDQYGEDALKEXXXXXXXAHDPFDI-XXXXXXXXXXXXXXXXXXXXXXXEDVIHPLKVSLEDLYNGTSXKLSLSRNVIXXXXXXXXXXXXXXXXXXXXQGSGMKVSIRHLGPSMIXQMQHPCNECKGTGETSMTRIAGHHVRVRRSIPRKN-LGGIVEKGIK 739
            MFGR PKKSDNTKYYEILGVSKNASQDDLKKAYRKAAIKNHPDKGGDPEKFKELAQAYEVLSDP+KREIYDQYGEDALKE        HDPFDI                        EDVIHPLKVSLEDLYNGTS KLSLSRNVI                    QGSGMKVSIRHLGPSMI QMQHPCNECKGTGET   +      +  + +  K  L  IVEKG++
Sbjct:    1 MFGR-PKKSDNTKYYEILGVSKNASQDDLKKAYRKAAIKNHPDKGGDPEKFKELAQAYEVLSDPEKREIYDQYGEDALKE-GMGGGGGHDPFDIFQSFFGGSPFGGGGSSRGRRQRRGEDVIHPLKVSLEDLYNGTSKKLSLSRNVICSKCKGKGSKSGASMKCPGCQGSGMKVSIRHLGPSMIQQMQHPCNECKGTGETINDKDRCSQCKGEKVVQEKKVLEVIVEKGMQ 229

[Close]

BLAST of EY728479 vs. ExPASy Swiss-Prot
Match: DNJH_ATRNU (DnaJ protein homolog ANJ1 OS=Atriplex nummularia PE=2 SV=1)

HSP 1 Score: 256.914 bits (655), Expect = 9.441e-68
Identity = 138/231 (59.74%), Postives = 155/231 (67.10%), Query Frame = 2

Query:   53 MFGRAPKKSDNTKYYEILGVSKNASQDDLKKAYRKAAIKNHPDKGGDPEKFKELAQAYEVLSDPQKREIYDQYGEDALKEXXXXXXXAHDPFDI-XXXXXXXXXXXXXXXXXXXXXXXEDVIHPLKVSLEDLYNGTSXKLSLSRNVIXXXXXXXXXXXXXXXXXXXXQGSGMKVSIRHLGPSMIXQMQHPCNECKGTGETSMTRIAGHHVRVRRSIPRKN-LGGIVEKGIK 739
            MFGRAPKKSD+T+YYEILGV K+AS +DLKKAY+KAAIKNHPDKGGDPEKFKELA AYEVLSDP+KREIYDQYGEDALKE        HDPFDI                        EDV+HPLKVSLEDL+ GT+ KLSLSRNVI                    QG+GMKVSIRHLGPSMI QMQHPCNECKGTGET   +      +  + +  K  L  +VEKG++
Sbjct:    1 MFGRAPKKSDSTRYYEILGVPKDASPEDLKKAYKKAAIKNHPDKGGDPEKFKELAHAYEVLSDPEKREIYDQYGEDALKEGMGGGGGMHDPFDIFQSFFGGSPFGGVGSSRGRRQRRGEDVVHPLKVSLEDLFTGTTKKLSLSRNVICSKCTGKGSKSGASMKCSGCQGTGMKVSIRHLGPSMIQQMQHPCNECKGTGETINDKDRCPQCKGEKVVQEKKVLEVVVEKGMQ 231

[Close]

BLAST of EY728479 vs. ExPASy Swiss-Prot
Match: DNJH2_ALLPO (DnaJ protein homolog 2 OS=Allium porrum GN=LDJ2 PE=2 SV=1)

HSP 1 Score: 256.914 bits (655), Expect = 9.441e-68
Identity = 136/231 (58.87%), Postives = 154/231 (66.67%), Query Frame = 2

Query:   53 MFGRAPKKSDNTKYYEILGVSKNASQDDLKKAYRKAAIKNHPDKGGDPEKFKELAQAYEVLSDPQKREIYDQYGEDALKEXXXXXXXAHDPFDI-XXXXXXXXXXXXXXXXXXXXXXXEDVIHPLKVSLEDLYNGTSXKLSLSRNVIXXXXXXXXXXXXXXXXXXXXQGSGMKVSIRHLGPSMIXQMQHPCNECKGTGETSMTRIAGHHVRVRRSIPRKNLGGI-VEKGIK 739
            MFGRAPKKSDNTKYYE+LGVSKNA+ +DLKKAYRKAAIKNHPDKGGDPEKFKE+ QAYEVL+DP+KREIYDQYGE+ LKE        HDPFDI                        EDV+HPLKVSLEDLYNGTS KLSLSRNV+                    QGSGMKVSIR LGP MI QMQHPCNECKGTGE    +      +  + + +K +  + VEKG++
Sbjct:    1 MFGRAPKKSDNTKYYEVLGVSKNATPEDLKKAYRKAAIKNHPDKGGDPEKFKEIGQAYEVLNDPEKREIYDQYGEEGLKEGMGGGGGVHDPFDIFQSFFGGGGFGGGGSSRGRRQRRGEDVVHPLKVSLEDLYNGTSKKLSLSRNVLCTKCKGKGSKSGASMNCASCQGSGMKVSIRQLGPGMIQQMQHPCNECKGTGEMISDKDRCPQCKGEKVVQQKKVLEVHVEKGMQ 231

[Close]

BLAST of EY728479 vs. ExPASy Swiss-Prot
Match: DNAJ3_ARATH (Chaperone protein dnaJ 3 OS=Arabidopsis thaliana GN=ATJ3 PE=1 SV=2)

HSP 1 Score: 251.136 bits (640), Expect = 5.180e-66
Identity = 137/231 (59.31%), Postives = 151/231 (65.37%), Query Frame = 2

Query:   53 MFGRAP-KKSDNTKYYEILGVSKNASQDDLKKAYRKAAIKNHPDKGGDPEKFKELAQAYEVLSDPQKREIYDQYGEDALKEXXXXXXXAHDPFDIXXXXXXXXXXXXXXXXXXXXXXXEDVIHPLKVSLEDLYNGTSXKLSLSRNVIXXXXXXXXXXXXXXXXXXXXQGSGMKVSIRHLGPSMIXQMQHPCNECKGTGETSMTRIAGHHVRVRRSIPRKNLGGI-VEKGIK 739
            MFGR P KKSDNTK+YEILGV K+AS +DLKKAY+KAAIKNHPDKGGDPEKFKELAQAYEVLSDP+KREIYDQYGEDALKE        HDPFDI                       EDV+HPLKVSLED+Y GT  KLSLSRN +                    QGSGMKVSIR LGP MI QMQH CNECKGTGET   R      +  + IP K +  + VEKG++
Sbjct:    1 MFGRGPSKKSDNTKFYEILGVPKSASPEDLKKAYKKAAIKNHPDKGGDPEKFKELAQAYEVLSDPEKREIYDQYGEDALKEGMGGGGGGHDPFDIFSSFFGGGPFGGNTSRQRRQRRGEDVVHPLKVSLEDVYLGTMKKLSLSRNALCSKCNGKGSKSGASLKCGGCQGSGMKVSIRQLGPGMIQQMQHACNECKGTGETINDRDRCPQCKGDKVIPEKKVLEVNVEKGMQ 231

[Close]

BLAST of EY728479 vs. ExPASy Swiss-Prot
Match: DNAJ2_ARATH (Chaperone protein dnaJ 2 OS=Arabidopsis thaliana GN=ATJ2 PE=1 SV=2)

HSP 1 Score: 233.802 bits (595), Expect = 8.559e-61
Identity = 127/232 (54.74%), Postives = 148/232 (63.79%), Query Frame = 2

Query:   53 MFGRAP-KKSDNTKYYEILGVSKNASQDDLKKAYRKAAIKNHPDKGGDPEKFKELAQAYEVLSDPQKREIYDQYGEDALKEXXXXXXXAHDPFDI-XXXXXXXXXXXXXXXXXXXXXXXEDVIHPLKVSLEDLYNGTSXKLSLSRNVIXXXXXXXXXXXXXXXXXXXXQGSGMKVSIRHLGPSMIXQMQHPCNECKGTGETSMTRIAGHHVRVRRSIPRKNLGGI-VEKGIK 739
            MFGR P +KSDNTK+YEILGV K A+ +DLKKAY+KAAIKNHPDKGGDPEKFKELAQAYEVLSDP+KREIYDQYGEDALKE        HDPFDI                        EDV+HPLKVSLED+Y GT+ KLSLSR  +                    QGSGMK+SIR  GP M+ Q+QH CN+CKGTGET   R      +  + +  K +  + VEKG++
Sbjct:    1 MFGRGPSRKSDNTKFYEILGVPKTAAPEDLKKAYKKAAIKNHPDKGGDPEKFKELAQAYEVLSDPEKREIYDQYGEDALKEGMGGGGGGHDPFDIFSSFFGSGGHPFGSHSRGRRQRRGEDVVHPLKVSLEDVYLGTTKKLSLSRKALCSKCNGKGSKSGASMKCGGCQGSGMKISIRQFGPGMMQQVQHACNDCKGTGETINDRDRCPQCKGEKVVSEKKVLEVNVEKGMQ 232

[Close]

BLAST of EY728479 vs. ExPASy Swiss-Prot
Match: DNJH1_ALLPO (DnaJ protein homolog 1 (Fragment) OS=Allium porrum GN=DNAJ1 PE=2 SV=1)

HSP 1 Score: 219.55 bits (558), Expect = 1.590e-60
Identity = 116/178 (65.17%), Postives = 121/178 (67.98%), Query Frame = 2

Query:  116 KNASQDDLKKAYRKAAIKNHPDKGGDPEKFKELAQAYEVLSDPQKREIYDQYGEDALKEXXXXXXXAHDPFDI-XXXXXXXXXXXXXXXXXXXXXXXEDVIHPLKVSLEDLYNGTSXKLSLSRNVIXXXXXXXXXXXXXXXXXXXXQGSGMKVSIRHLGPSMIXQMQHPCNECKGTGE 646
            KNAS DDLKKAYRKAAIKNHPDKGGDPEKFKELAQAY+VLSDP+KREIYDQYGEDALKE        HDPFDI                        EDV+HPLKVSLE+LYNGTS KLSLSRNVI                    QGSGMKVSIR LGP MI QMQHPCN+CKGTGE
Sbjct:    1 KNASPDDLKKAYRKAAIKNHPDKGGDPEKFKELAQAYDVLSDPEKREIYDQYGEDALKEGMGGGGGDHDPFDIFQSFFGGGGFGGGGSSRGRRQRRGEDVVHPLKVSLEELYNGTSKKLSLSRNVICSKCNGKGSKSGASMRCASCQGSGMKVSIRQLGPGMIQQMQHPCNDCKGTGE 178

HSP 2 Score: 35.4242 bits (80), Expect = 1.590e-60
Identity = 17/26 (65.38%), Postives = 18/26 (69.23%), Query Frame = 1

Query:  631 QGYWRNINDKDRWPPCKGEKVDPEKK 708
            +G    INDKDR P CKGEKV  EKK
Sbjct:  174 KGTGEMINDKDRCPLCKGEKVVQEKK 199

[Close]

BLAST of EY728479 vs. ExPASy Swiss-Prot
Match: DNJA4_MOUSE (DnaJ homolog subfamily A member 4 OS=Mus musculus GN=Dnaja4 PE=2 SV=1)

HSP 1 Score: 149.443 bits (376), Expect = 3.622e-37
Identity = 83/188 (44.15%), Postives = 108/188 (57.45%), Query Frame = 2

Query:   86 TKYYEILGVSKNASQDDLKKAYRKAAIKNHPDKGGDP-EKFKELAQAYEVLSDPQKREIYDQYGEDALKEXXXXXXXAHDPFDIXXXXXXXXXXXXXXXXXXXXXXXEDVIHPLKVSLEDLYNGTSXKLSLSRNVIXXXXXXXXXXXXXXXXXXXXQGSGMKVSIRHLGPSMIXQMQHPCNECKGTGE 646
            T+YY+ILGV  +AS +++KKAYRK A+K HPDK  D  EKFK ++QAYEVLSDP+KR+IYDQ GE A+KE          P DI                       ++V+H L V+LEDLYNG + KL+L +NVI                    +G GM+V I+ +GP M+ Q+Q  C ECKG GE
Sbjct:    5 TQYYDILGVKPSASPEEIKKAYRKLALKYHPDKNPDEGEKFKLISQAYEVLSDPKKRDIYDQGGEQAIKEGGSGSPSFSSPMDI------FDMFFGGGGRMTRERRGKNVVHQLSVTLEDLYNGITKKLALQKNVICEKCEGIGGKKGSVEKCPLCKGRGMQVHIQQIGPGMVQQIQTVCIECKGQGE 186

HSP 2 Score: 27.335 bits (59), Expect = 3.622e-37
Identity = 13/26 (50.00%), Postives = 14/26 (53.85%), Query Frame = 1

Query:  631 QGYWRNINDKDRWPPCKGEKVDPEKK 708
            +G    IN KDR   C G KV  EKK
Sbjct:  182 KGQGERINPKDRCENCSGAKVTREKK 207

[Close]

BLAST of EY728479 vs. ExPASy Swiss-Prot
Match: DNJA2_RAT (DnaJ homolog subfamily A member 2 OS=Rattus norvegicus GN=Dnaja2 PE=2 SV=1)

HSP 1 Score: 149.058 bits (375), Expect = 1.769e-36
Identity = 80/189 (42.33%), Postives = 107/189 (56.61%), Query Frame = 2

Query:   83 NTKYYEILGVSKNASQDDLKKAYRKAAIKNHPDKGGDP-EKFKELAQAYEVLSDPQKREIYDQYGEDALKEXXXXXXXAHDPFDIXXXXXXXXXXXXXXXXXXXXXXXEDVIHPLKVSLEDLYNGTSXKLSLSRNVIXXXXXXXXXXXXXXXXXXXXQGSGMKVSIRHLGPSMIXQMQHPCNECKGTGE 646
            +TK Y+ILGV   AS+++LKKAYRK A + HPDK  +  +KFKE++ AYEVLS+P+KRE+YD+YGE  L+E         D F                         ED++HPLKVSLEDLYNG + KL LS+NV+                    +G G+++ IR L P M+ QMQ  C++C G GE
Sbjct:    6 DTKLYDILGVPPGASENELKKAYRKLAKEYHPDKNPNAGDKFKEISFAYEVLSNPEKRELYDRYGEQGLREGSGGGGGMDDIFSHIFGGGLFGFMGNQSRSRNGRRRGEDMMHPLKVSLEDLYNGKTTKLQLSKNVLCSACSGQGGKSGAVQKCSACRGRGVRIMIRQLAPGMVQQMQSVCSDCNGEGE 194

HSP 2 Score: 25.409 bits (54), Expect = 1.769e-36
Identity = 11/20 (55.00%), Postives = 14/20 (70.00%), Query Frame = 1

Query:  649 INDKDRWPPCKGEKVDPEKK 708
            IN+KDR   C+G+KV  E K
Sbjct:  196 INEKDRCKKCEGKKVIKEVK 215

[Close]

BLAST of EY728479 vs. ExPASy Swiss-Prot
Match: DNJA2_MOUSE (DnaJ homolog subfamily A member 2 OS=Mus musculus GN=Dnaja2 PE=1 SV=1)

HSP 1 Score: 149.058 bits (375), Expect = 1.769e-36
Identity = 80/189 (42.33%), Postives = 107/189 (56.61%), Query Frame = 2

Query:   83 NTKYYEILGVSKNASQDDLKKAYRKAAIKNHPDKGGDP-EKFKELAQAYEVLSDPQKREIYDQYGEDALKEXXXXXXXAHDPFDIXXXXXXXXXXXXXXXXXXXXXXXEDVIHPLKVSLEDLYNGTSXKLSLSRNVIXXXXXXXXXXXXXXXXXXXXQGSGMKVSIRHLGPSMIXQMQHPCNECKGTGE 646
            +TK Y+ILGV   AS+++LKKAYRK A + HPDK  +  +KFKE++ AYEVLS+P+KRE+YD+YGE  L+E         D F                         ED++HPLKVSLEDLYNG + KL LS+NV+                    +G G+++ IR L P M+ QMQ  C++C G GE
Sbjct:    6 DTKLYDILGVPPGASENELKKAYRKLAKEYHPDKNPNAGDKFKEISFAYEVLSNPEKRELYDRYGEQGLREGSGGGGGMDDIFSHIFGGGLFGFMGNQSRSRNGRRRGEDMMHPLKVSLEDLYNGKTTKLQLSKNVLCSACSGQGGKSGAVQKCSACRGRGVRIMIRQLAPGMVQQMQSVCSDCNGEGE 194

HSP 2 Score: 25.409 bits (54), Expect = 1.769e-36
Identity = 11/20 (55.00%), Postives = 14/20 (70.00%), Query Frame = 1

Query:  649 INDKDRWPPCKGEKVDPEKK 708
            IN+KDR   C+G+KV  E K
Sbjct:  196 INEKDRCKKCEGKKVIKEVK 215

[Close]

BLAST of EY728479 vs. ExPASy Swiss-Prot
Match: DNJA2_HUMAN (DnaJ homolog subfamily A member 2 OS=Homo sapiens GN=DNAJA2 PE=1 SV=1)

HSP 1 Score: 149.058 bits (375), Expect = 1.769e-36
Identity = 80/189 (42.33%), Postives = 107/189 (56.61%), Query Frame = 2

Query:   83 NTKYYEILGVSKNASQDDLKKAYRKAAIKNHPDKGGDP-EKFKELAQAYEVLSDPQKREIYDQYGEDALKEXXXXXXXAHDPFDIXXXXXXXXXXXXXXXXXXXXXXXEDVIHPLKVSLEDLYNGTSXKLSLSRNVIXXXXXXXXXXXXXXXXXXXXQGSGMKVSIRHLGPSMIXQMQHPCNECKGTGE 646
            +TK Y+ILGV   AS+++LKKAYRK A + HPDK  +  +KFKE++ AYEVLS+P+KRE+YD+YGE  L+E         D F                         ED++HPLKVSLEDLYNG + KL LS+NV+                    +G G+++ IR L P M+ QMQ  C++C G GE
Sbjct:    6 DTKLYDILGVPPGASENELKKAYRKLAKEYHPDKNPNAGDKFKEISFAYEVLSNPEKRELYDRYGEQGLREGSGGGGGMDDIFSHIFGGGLFGFMGNQSRSRNGRRRGEDMMHPLKVSLEDLYNGKTTKLQLSKNVLCSACSGQGGKSGAVQKCSACRGRGVRIMIRQLAPGMVQQMQSVCSDCNGEGE 194

HSP 2 Score: 25.409 bits (54), Expect = 1.769e-36
Identity = 11/20 (55.00%), Postives = 14/20 (70.00%), Query Frame = 1

Query:  649 INDKDRWPPCKGEKVDPEKK 708
            IN+KDR   C+G+KV  E K
Sbjct:  196 INEKDRCKKCEGKKVIKEVK 215

The following BLAST results are available for this feature:

BLAST of EY728479 vs. ExPASy Swiss-Prot

BLAST of EY728479 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
DNJH_CUCSA	4.095e-71	65.37	DnaJ protein homolog OS=Cucumis sativus GN=DNAJ1 P...	[more]
DNJH_ATRNU	9.441e-68	59.74	DnaJ protein homolog ANJ1 OS=Atriplex nummularia P...	[more]
DNJH2_ALLPO	9.441e-68	58.87	DnaJ protein homolog 2 OS=Allium porrum GN=LDJ2 PE...	[more]
DNAJ3_ARATH	5.180e-66	59.31	Chaperone protein dnaJ 3 OS=Arabidopsis thaliana G...	[more]
DNAJ2_ARATH	8.559e-61	54.74	Chaperone protein dnaJ 2 OS=Arabidopsis thaliana G...	[more]
DNJH1_ALLPO	1.590e-60	65.17	DnaJ protein homolog 1 (Fragment) OS=Allium porrum...	[more]
DNJA4_MOUSE	3.622e-37	44.15	DnaJ homolog subfamily A member 4 OS=Mus musculus ...	[more]
DNJA2_RAT	1.769e-36	42.33	DnaJ homolog subfamily A member 2 OS=Rattus norveg...	[more]
DNJA2_MOUSE	1.769e-36	42.33	DnaJ homolog subfamily A member 2 OS=Mus musculus ...	[more]
DNJA2_HUMAN	1.769e-36	42.33	DnaJ homolog subfamily A member 2 OS=Homo sapiens ...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C3-704-004-C12-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY728479 ID=EY728479; Name=EY728479; organism=Citrus sinensis; type=EST; length=803bp
CCACGCGTCCGCGCGTCTACTCTGAAGATCTGAAATTTTTTAGTACAAGA
AGATGTTCGGAAGAGCGCCAAAGAAAAGCGATAACACAAAGTACTATGAG
ATTCTCGGAGTCTCGAAAAACGCTTCGCAAGATGATCTGAAGAAGGCTTA
TAGGAAAGCCGCCATCAAAAACCATCCTGACAAGGGCGGTGATCCTGAGA
AGTTTAAAGAGTTGGCACAAGCGTATGAGGTTCTGAGTGACCCACAGAAG
CGTGAAATATATGATCAATACGGCGAGGATGCGCTTAAAGAGGGAATGGG
TGGTGGTGGTGGTGCCCATGACCCATTCGACATATTCCAATCCTTCTTCG
GTGGCAGCCCATTTGGTGGTGGGAGCAGCAGAGGAAGAAGGCAAAGGAGG
GGAGAGGACGTAATCCATCCCCTCAAGGTTTCGTTGGAGGATCTGTACAA
TGGGACATCTAANAAGCTCTCGCTCTCCCGCAATGTAATCTGCACCAAGT
GCAAAGGGAAGGGGTCTAAGTCAGGTGCATCAATGAAATGTTCTGGCTGC
CAGGGATCTGGAATGAAAGTTTCAATAAGACACCTTGGCCCCTCTATGAT
TCANCAAATGCAGCATCCTTGCAATGAGTGCAAGGGTACTGGAGAAACAT
CAATGACAAGGATCGCTGGCCACCATGTAAGGGTGAGAAGGTCGATCCCG
AGAAAAAATCTTGGAGGTATTGTTGAAAAGGGGATCAAATGGACNAAAAT
ACTTCCTCGAAACTGAGAACGCCCACCGCCCGTGACTGTTTGTCTCACAA
AGA