Characterization of a Citrus R2R3-MYB Transcription Factor that Regulates the Flavonol and Hydroxycinnamic Acid Biosynthesis

Publication Overview
TitleCharacterization of a Citrus R2R3-MYB Transcription Factor that Regulates the Flavonol and Hydroxycinnamic Acid Biosynthesis
AuthorsLiu C, Long J, Zhu K, Liu L, Yang W, Zhang H, Li L, Xu Q, Deng X
TypeJournal Article
Journal NameScientific reports
Volume6
Year2016
Page(s)25352
CitationLiu C, Long J, Zhu K, Liu L, Yang W, Zhang H, Li L, Xu Q, Deng X. Characterization of a Citrus R2R3-MYB Transcription Factor that Regulates the Flavonol and Hydroxycinnamic Acid Biosynthesis. Scientific reports. 2016; 6:25352.

Abstract

Flavonols and hydroxycinnamic acids are important phenylpropanoid metabolites in plants. In this study, we isolated and characterized a citrus R2R3-MYB transcription factor CsMYBF1, encoding a protein belonging to the flavonol-specific MYB subgroup. Ectopic expression of CsMYBF1 in tomato led to an up-regulation of a series of genes involved in primary metabolism and the phenylpropanoid pathway, and induced a strong accumulation of hydroxycinnamic acid compounds but not the flavonols. The RNAi suppression of CsMYBF1 in citrus callus caused a down-regulation of many phenylpropanoid pathway genes and reduced the contents of hydroxycinnamic acids and flavonols. Transactivation assays indicated that CsMYBF1 activated several promoters of phenylpropanoid pathway genes in tomato and citrus. Interestingly, CsMYBF1 could activate the CHS gene promoter in citrus, but not in tomato. Further examinations revealed that the MYBPLANT cis-elements were essential for CsMYBF1 in activating phenylpropanoid pathway genes. In summary, our data indicated that CsMYBF1 possessed the function in controlling the flavonol and hydroxycinnamic acid biosynthesis, and the regulatory differences in the target metabolite accumulation between two species may be due to the differential activation of CHS promoters by CsMYBF1. Therefore, CsMYBF1 constitutes an important gene source for the engineering of specific phenylpropanoid components.

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This publication contains information about 2 features:
Feature NameUniquenameType
KT727073KT727073.1region
Al0329Al0329genetic_marker
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Additional details for this publication include:
Property NameValue
DOI10.1038/srep25352
Elocation10.1038/srep25352
Publication ModelElectronic
ISSN2045-2322
eISSN2045-2322
Publication Date2016
Journal AbbreviationSci Rep
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountryEngland
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PMID: PubMedPMID:27162196