Regulation of urinary crystal inhibiting proteins and inflammatory genes by lemon peel extract and formulated citrus bioflavonoids on ethylene glycol induced urolithic rats

Publication Overview
TitleRegulation of urinary crystal inhibiting proteins and inflammatory genes by lemon peel extract and formulated citrus bioflavonoids on ethylene glycol induced urolithic rats
AuthorsSridharan B, Mehra Y, Ganesh RN, Pragasam V
TypeJournal Article
Journal NameFood and chemical toxicology : an international journal published for the British Industrial Biological Research Association
Year2016
CitationSridharan B, Mehra Y, Ganesh RN, Pragasam V. Regulation of urinary crystal inhibiting proteins and inflammatory genes by lemon peel extract and formulated citrus bioflavonoids on ethylene glycol induced urolithic rats. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 2016 May 27.

Abstract

The objective of this study is to check the regulation of crystal matrix proteins and inflammatory mediators by citrus bioflavonoids (CB) and Lemon peel (LP) extract in hyperoxaluric rats. The animals were divided into six groups with 6 animals each. Group 1: Control, Group 2: Urolithic (Ethylene glycol (EG)-0.75%); Group 3& 5: Preventive study (EG + CB (20 mg/kg body weight) and LP (100 mg/kg body weight) extract administration from 0th-7th week) respectively; Group 4 & 6: Curative study (EG + CB and LP extract administration from 4th-7th week) respectively by oral administration. Urinary lithogenic factors (Calcium, oxalate, phosphate and citrate) were normalized in CB & LP supplemented rats, while serum parameters revealed the nephroprotective nature of the intervening agents compared to urolithic rats (p < 0.001). Immunoblotting studies showed significantly increased expression of THP, osteopontin and transferrin in kidneys of urolithic rats (p < 0.001), while preventive and curative study showed near normal expression of these proteins. Expression of NF-κB, TNF-α and IL-6 were raised significantly (p < 0.001), while a very minimal increase in MCP-1 expression was observed in urolithic rats compared to control. Hence, supplementation of CB and LP reduced the crystal promoting factors and provides protection from crystal induced renal damage.

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Additional details for this publication include:
Property NameValue
Publication ModelPrint-Electronic
ISSN1873-6351
eISSN1873-6351
Publication Date2016 May 27
Journal AbbreviationFood Chem. Toxicol.
Elocation10.1016/j.fct.2016.05.013
CopyrightCopyright © 2016. Published by Elsevier Ltd.
LanguageEnglish
Language AbbrENG
Publication TypeJournal Article
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PMID: PubMedPMID:27241030