Serological detection of 'Candidatus Liberibacter asiaticus' in citrus, and identification by GeLC-MS/MS of a chaperone protein responding to cellular pathogens

Publication Overview
TitleSerological detection of 'Candidatus Liberibacter asiaticus' in citrus, and identification by GeLC-MS/MS of a chaperone protein responding to cellular pathogens
AuthorsDing F, Duan Y, Yuan Q, Shao J, Hartung JS
TypeJournal Article
Journal NameScientific reports
Volume6
Year2016
Page(s)29272
CitationDing F, Duan Y, Yuan Q, Shao J, Hartung JS. Serological detection of 'Candidatus Liberibacter asiaticus' in citrus, and identification by GeLC-MS/MS of a chaperone protein responding to cellular pathogens. Scientific reports. 2016; 6:29272.

Abstract

We describe experiments with antibodies against 'Candidatus Liberibacter asiaticus used to detect the pathogen in infected plants. We used scFv selected to bind epitopes exposed on the surface of the bacterium in tissue prints, with secondary monoclonal antibodies directed at a FLAG epitope included at the carboxyl end of the scFv. Unexpectedly, the anti-FLAG secondary antibody produced positive results with CaLas diseased samples when the primary scFv were not used. The anti-FLAG monoclonal antibody (Mab) also identified plants infected with other vascular pathogens. We then identified a paralogous group of secreted chaperone proteins in the HSP-90 family that contained the amino acid sequence DDDDK identical to the carboxy-terminal sequence of the FLAG epitope. A rabbit polyclonal antibody against one of the same epitopes combined with a goat anti-rabbit secondary antibody produced very strong purple color in individual phloem cells, as expected for this pathogen. These results were entirely specific for CaLas-infected citrus. The simplicity, cost and ability to scale the tissue print assay makes this an attractive assay to complement PCR-based assays currently in use. The partial FLAG epitope may itself be useful as a molecular marker for the rapid screening of citrus plants for the presence of vascular pathogens.

Features
This publication contains information about 1 features:
Feature NameUniquenameType
Bf0218Bf0218genetic_marker
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Additional details for this publication include:
Property NameValue
Publication ModelElectronic
ISSN2045-2322
eISSN2045-2322
Publication Date2016
Journal AbbreviationSci Rep
Elocation10.1038/srep29272
LanguageEnglish
Language AbbrENG
Publication TypeJournal Article
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PMID: PubMedPMID:27381064