Convergent evolution of caffeine in plants by co-option of exapted ancestral enzymes

Publication Overview
TitleConvergent evolution of caffeine in plants by co-option of exapted ancestral enzymes
AuthorsHuang R, O'Donnell AJ, Barboline JJ, Barkman TJ
TypeJournal Article
Journal NameProceedings of the National Academy of Sciences of the United States of America
Volume113
Issue38
Year2016
Page(s)10613-8
CitationHuang R, O'Donnell AJ, Barboline JJ, Barkman TJ. Convergent evolution of caffeine in plants by co-option of exapted ancestral enzymes. Proceedings of the National Academy of Sciences of the United States of America. 2016 Sep 20; 113(38):10613-8.

Abstract

Convergent evolution is a process that has occurred throughout the tree of life, but the historical genetic and biochemical context promoting the repeated independent origins of a trait is rarely understood. The well-known stimulant caffeine, and its xanthine alkaloid precursors, has evolved multiple times in flowering plant history for various roles in plant defense and pollination. We have shown that convergent caffeine production, surprisingly, has evolved by two previously unknown biochemical pathways in chocolate, citrus, and guaran√° plants using either caffeine synthase- or xanthine methyltransferase-like enzymes. However, the pathway and enzyme lineage used by any given plant species is not predictable from phylogenetic relatedness alone. Ancestral sequence resurrection reveals that this convergence was facilitated by co-option of genes maintained over 100 million y for alternative biochemical roles. The ancient enzymes of the Citrus lineage were exapted for reactions currently used for various steps of caffeine biosynthesis and required very few mutations to acquire modern-day enzymatic characteristics, allowing for the evolution of a complete pathway. Future studies aimed at manipulating caffeine content of plants will require the use of different approaches given the metabolic and genetic diversity revealed by this study.

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This publication contains information about 1 features:
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Ov0117Ov0117genetic_marker
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Additional details for this publication include:
Property NameValue
Publication ModelPrint
ISSN1091-6490
eISSN1091-6490
Publication Date2016 Sep 20
Journal AbbreviationProc. Natl. Acad. Sci. U.S.A.
DOI10.1073/pnas.1602575113
Elocation10.1073/pnas.1602575113
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountryUnited States
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PMID: PubMedPMID:27638206