Assessment of the origin of new citrus tetraploid hybrids (2n = 4x) by means of SSR markers and PCR based dosage effects

Publication Overview
TitleAssessment of the origin of new citrus tetraploid hybrids (2n = 4x) by means of SSR markers and PCR based dosage effects
AuthorsFerrante SP, Lucretti S, Reale S, De Patrizio A, Abbate L, Tusa N, Scarano M
TypeJournal Article
Journal NameEuphytica
Volume173
Issue2
Year2010
Page(s)223-233
CitationFerrante SP, Lucretti S, Reale S, De Patrizio A, Abbate L, Tusa N, Scarano M. Assessment of the origin of new citrus tetraploid hybrids (2n = 4x) by means of SSR markers and PCR based dosage effects. Euphytica. 2010; 173(2):223-233.

Abstract

We report the accurate determination of the allelic configurations of a total of eight new citrus tetraploid hybrids by means of SSR analysis, coupled with capillary electrophoresis, and PCR based dosage effects. Tetraploid hybrids were spontaneously obtained from different interploid crosses (2x × 4x) between diploid ‘Femminello' lemon and the allotetraploid somatic hybrid (2n = 4x = 36) ‘Key' lime + ‘Valencia' orange, and between diploid ‘Wilking' and ‘Fortune' mandarins and an autotetraploid ‘Dancy' mandarin (2n = 4x = 36). To understand the opportunity to employ them in further backcross programs, the cytological mechanisms underlying their ploidy level were unambiguously determined using six SSR primers. PCR conditions were optimized and skewness in template/product ratios were verified. Tetraploid allelic configurations were determined from PCR based dosage effects using electropherogram peak heights to estimate the copy number per allele. In all the tetraploid hybrids we found out that diploginy (2n eggs) has occurred, contributing the extra haploid genome in the tetraploids. According to the marker genotypes, it was further inferred that the 2n eggs in ‘Femminello' lemon resulted from first division restitution (FDR), while in ‘Wilking' and ‘Fortune' mandarins 2n eggs occurred in second division restitution (SDR). These new genotypes, with their improved genetic female background, can be therefore considered very valuable in our citrus genetic improvement program as pollen donors in backcrosses suitable to eliminate negative traits.
Features
This publication contains information about 4 features:
Feature NameUniquenameType
CAT01CAT01genetic_marker
GT03GT03genetic_marker
TAA3TAA3genetic_marker
TAA41TAA41genetic_marker
Stocks
This publication contains information about 10 stocks:
Stock NameUniquenameType
DancyDancycultivar
FortuneFortunecultivar
WilkingWilkingcultivar
KeylimeKeylimecultivar
ValenciaValenciacultivar
FemminelloFemminellocultivar
Dancy_x_FortuneDancy_x_Fortunepopulation
Dancy_x_WilkingDancy_x_Wilkingpopulation
Keylime_x_ValenciaKeylime_x_Valenciapopulation
(Keylime_x_Valencia)_x_Femminello(Keylime_x_Valencia)_x_Femminellopopulation
Properties
Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Publication Date2010
Published Location|||
Language Abbreng
Publication Model[electronic resource].
URLhttp://dx.doi.org/10.1007/s10681-009-0093-3
KeywordsCitrus, citrus fruits, fruit crops, tetraploidy, hybrids, interspecific hybridization, genetic markers, polymerase chain reaction, microsatellite repeats, gene dosage, alleles, backcrossing, ovules, haploidy, meiosis, cell division, species differences, plant genetic resources, molecular sequence data, first division restitution, second division restitution
Cross References
This publication is also available in the following databases:
DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:4352410