Multi-lineages of Shiikuwasha (Citrus depressa Hayata) evaluated by using whole chloroplast genome sequences and its bio-diversity in Okinawa, Japan

Publication Overview
TitleMulti-lineages of Shiikuwasha (Citrus depressa Hayata) evaluated by using whole chloroplast genome sequences and its bio-diversity in Okinawa, Japan
AuthorsIshikawa R, Badenoch N, Miyagi K, Medoruma K, Osada T, Onishi M
TypeJournal Article
Journal NameBreeding science
Volume66
Issue4
Year2016
Page(s)490-498
CitationIshikawa R, Badenoch N, Miyagi K, Medoruma K, Osada T, Onishi M. Multi-lineages of Shiikuwasha (Citrus depressa Hayata) evaluated by using whole chloroplast genome sequences and its bio-diversity in Okinawa, Japan. Breeding science. 2016 Sep; 66(4):490-498.

Abstract

Shiikuwasha (Citrus depressa Hayata) is distributed from the South-west of the Japanese archipelago to Taiwan. In this study, re-sequencing against the orange (C. sinensis (L.) Osbeck) chloroplast genome was applied to one superior landrace of Shiikuwasha cultivated in Oku ward, Okinawa, Japan. The chloroplast genome of the landrace was estimated to comprise 160,118 bp, including 48 indels and 71 nucleotide substitutions against the reference genome. The presumptive chloroplast indels were confirmed by subsequent experiments, and these identified multiple maternal lineages among other landraces. Some of the orange SSR markers were available for genotyping of other superior landraces and were able to distinguish among them. These molecular markers were then applied for evaluation of genetic diversity among wild and cultivated Shiikuwasha accessions. Except for Oku ward, the cultivated populations were found to have lost their genetic diversity in comparison with wild populations. Groves in Oku ward maintained, or showed even higher genetic diversity than wild accessions in the surrounding areas by the force of villagers.

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Tf0142Tf0142genetic_marker
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Additional details for this publication include:
Property NameValue
Publication ModelPrint-Electronic
ISSN1344-7610
pISSN1344-7610
Publication Date2016 Sep
Journal AbbreviationBreed. Sci.
LanguageEnglish
Language AbbrENG
Publication TypeJournal Article
Journal CountryJapan
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PMID: PubMedPMID:27795674