Development and application of a multiplex real-time PCR assay as an indicator of potential allergenicity in citrus fruits

Publication Overview
TitleDevelopment and application of a multiplex real-time PCR assay as an indicator of potential allergenicity in citrus fruits
AuthorsWu J, Chen L, Lin D, Ma Z, Deng X
TypeJournal Article
Journal NameJournal of agricultural and food chemistry
Year2016
CitationWu J, Chen L, Lin D, Ma Z, Deng X. Development and application of a multiplex real-time PCR assay as an indicator of potential allergenicity in citrus fruits. Journal of agricultural and food chemistry. 2016 Oct 31.

Abstract

The effects of tissue type, harvest maturity and genetic factors on the expression of genes that related to citrus fruit allergies remain poorly understood. In the present study, a multiplex real-time PCR assay was developed to monitor the expression of citrus allergen genes individually with the advantages of much fewer sample requirements and simultaneously multiple target genes detection. Gene specific primer pairs and Taqman probes of three citrus allergen genes Cit s 1.01, Cit s 2.01, Cit s 3.01 and the house-keeping gene β-actin were designed based on gene sequence differences. The PCR results showed that differential expression patterns were found during the ripening process. The expression levels of Cit s 3.01 were much higher than those of Cit s 1.01 and Cit s 2.01 in both peel and pulp tissues among ten citrus cultivars. Data suggested that Kao Phuang Pummelo could be safely consumed with a potential low risk in allergenicity. Considering that assessing allergenicity is one of the tests in food safety, this assay might also facilitate the breeding and production of "allergy-friendly" citrus fruits.

Features
This publication contains information about 1 features:
Feature NameUniquenameType
Tf0149Tf0149genetic_marker
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Additional details for this publication include:
Property NameValue
Publication ModelPrint-Electronic
ISSN1520-5118
eISSN1520-5118
Publication Date2016 Oct 31
Journal AbbreviationJ. Agric. Food Chem.
LanguageEnglish
Language AbbrENG
Publication TypeJournal Article
Journal CountryUnited States
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PMID: PubMedPMID:27794596