Fine Mapping for Identification of Citrus Alternaria Brown Spot Candidate Resistance Genes and Development of New SNP Markers for Marker-Assisted Selection

Publication Overview
TitleFine Mapping for Identification of Citrus Alternaria Brown Spot Candidate Resistance Genes and Development of New SNP Markers for Marker-Assisted Selection
AuthorsCuenca J, Aleza P, Garcia-Lor A, Ollitrault P, Navarro L
TypeJournal Article
Journal NameFrontiers in plant science
Volume7
Year2016
Page(s)1948
CitationCuenca J, Aleza P, Garcia-Lor A, Ollitrault P, Navarro L. Fine Mapping for Identification of Citrus Alternaria Brown Spot Candidate Resistance Genes and Development of New SNP Markers for Marker-Assisted Selection. Frontiers in plant science. 2016; 7:1948.

Abstract

Alternaria brown spot (ABS) is a serious disease affecting susceptible citrus genotypes, which is a strong concern regarding citrus breeding programs. Resistance is conferred by a recessive locus (ABSr) previously located by our group within a 3.3 Mb genome region near the centromere in chromosome III. This work addresses fine-linkage mapping of this region for identifying candidate resistance genes and develops new molecular markers for ABS-resistance effective marker-assisted selection (MAS). Markers closely linked to ABSr locus were used for fine mapping using a 268-segregating diploid progeny derived from a heterozygous susceptible × resistant cross. Fine mapping limited the genomic region containing the ABSr resistance gene to 366 kb, flanked by markers at 0.4 and 0.7 cM. This region contains nine genes related to pathogen resistance. Among them, eight are resistance (R) gene homologs, with two of them harboring a serine/threonine protein kinase domain. These two genes along with a gene encoding a S-adenosyl-L-methionine-dependent-methyltransferase protein, should be considered as strong candidates for ABS-resistance. Moreover, the closest SNP was genotyped in 40 citrus varieties, revealing very high association with the resistant/susceptible phenotype. This new marker is currently used in our citrus breeding program for ABS-resistant parent and cultivar selection, at diploid, triploid and tetraploid level.

Features
This publication contains information about 9 features:
Feature NameUniquenameType
SNP01SNP01genetic_marker
SNP02SNP02genetic_marker
SNP03SNP03genetic_marker
SNP04SNP04genetic_marker
SNP05SNP05genetic_marker
SNP06SNP06genetic_marker
SNP07SNP07genetic_marker
SNP08SNP08genetic_marker
SNP09SNP09genetic_marker
Stocks
This publication contains information about 40 stocks:
Stock NameUniquenameType
ClemenulesClemenulescultivar
AnanaAnanacultivar
CampeonaCampeonacultivar
CarvahalCarvahalcultivar
DancyDancycultivar
EmperorEmperorcultivar
KingKingcultivar
PonkanPonkancultivar
ScarletScarletcultivar
TempleTemplecultivar
WillowleafWillowleafcultivar
DaisyDaisycultivar
EncoreEncorecultivar
FairchildFairchildcultivar
FallgloFallglocultivar
FortuneFortunecultivar
FremontFremontcultivar
Gold NuggetGold Nuggetcultivar
HoneyHoneycultivar
KaraKaracultivar
KinnowKinnowcultivar
MoncadaMoncadacultivar
NovaNovacultivar
OsceolaOsceolacultivar
PagePagecultivar

Pages

Properties
Additional details for this publication include:
Property NameValue
Publication ModelElectronic-eCollection
Publication Date2016
Journal AbbreviationFront Plant Sci
DOI10.3389/fpls.2016.01948
Elocation10.3389/fpls.2016.01948
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
Journal CountrySwitzerland
Cross References
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DatabaseAccession
PMID: PubMedPMID:28066498