EY685444 | Citrus Genome Database

Overview


Name	EY685444
Unique Name	EY685444
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	935

Libraries


Library Name	Type
Sweet orange bark, greenhouse plant	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY685444 vs. ExPASy Swiss-Prot
Match: CUTA_ARATH (Protein CutA, chloroplastic OS=Arabidopsis thaliana GN=CUTA PE=1 SV=1)

HSP 1 Score: 195.282 bits (495), Expect = 9.957e-52
Identity = 111/171 (64.91%), Postives = 136/171 (79.53%), Query Frame = 2

Query:   53 MASTLCTRASSLVSSPTIRRRLPLVGAFCMLS-LGLTNLCPTLSSSLKTGSAPS---LPFFGSKFSNQARAKSSNTIKMEGNRNTVPSIVVYVTVPNKEAGKKLAESIVKAKLAACVNRVPGIESVYEWKGEIQTDAEELLIIKTRQSLLETLTEHVKANHEYDVPEVIAL 553
            MAS+L TR S+++ S   RR  P+VGAFC+LS L +++L  + SS  K+G A S   +P   SKFS++A    S++I+ME +  TVPSIVVYVTVPN+EAGKKLA SIV+ KLAACVN VPGIESVYEW+G++Q+D+EELLIIKTRQSLLE LTEHV ANHEYDVPEVIAL
Sbjct:    1 MASSLTTRLSAVIGS---RRSFPIVGAFCVLSTLSISSL--SSSSPFKSGCAQSFSVVPLLRSKFSSKA---FSSSIRMEESSKTVPSIVVYVTVPNREAGKKLANSIVQEKLAACVNIVPGIESVYEWEGKVQSDSEELLIIKTRQSLLEPLTEHVNANHEYDVPEVIAL 163

HSP 2 Score: 30.4166 bits (67), Expect = 9.957e-52
Identity = 12/14 (85.71%), Postives = 12/14 (85.71%), Query Frame = 3

Query:  555 PITGGSQPYLEWLK 596
            PITGGS  YLEWLK
Sbjct:  164 PITGGSDKYLEWLK 177

[Close]

BLAST of EY685444 vs. ExPASy Swiss-Prot
Match: CUTA1_ORYSJ (Protein CutA 1, chloroplastic OS=Oryza sativa subsp. japonica GN=CUTA1 PE=1 SV=1)

HSP 1 Score: 163.696 bits (413), Expect = 4.982e-41
Identity = 91/159 (57.23%), Postives = 106/159 (66.67%), Query Frame = 2

Query:   77 ASSLVSSPTIRRRLPLVGAFCMLSLGLTNLCPTLSSSLKTGSAPSLPFFGSKFSNQARAKSSNTIKMEGNRNTVPSIVVYVTVPNKEAGKKLAESIVKAKLAACVNRVPGIESVYEWKGEIQTDAEELLIIKTRQSLLETLTEHVKANHEYDVPEVIAL 553
            A++ +S   +RRR P+ GA   LSLG                          F+      SS + +ME    TVPSIVVYVTVPNKEAGK+LA SI+  KLAACVN VPGIESVY W+G++QTDAEELLIIKTR+SLL+ LTEHVKANHEYDVPEVIAL
Sbjct:   24 AAAGLSPLLLRRRAPIAGALLFLSLGA-------------------------FAGVRSLSSSASARMESTSTTVPSIVVYVTVPNKEAGKRLAGSIISEKLAACVNIVPGIESVYWWEGKVQTDAEELLIIKTRESLLDALTEHVKANHEYDVPEVIAL 157

HSP 2 Score: 26.1794 bits (56), Expect = 4.982e-41
Identity = 10/14 (71.43%), Postives = 11/14 (78.57%), Query Frame = 3

Query:  555 PITGGSQPYLEWLK 596
            PI GG+  YLEWLK
Sbjct:  158 PIKGGNLKYLEWLK 171

[Close]

BLAST of EY685444 vs. ExPASy Swiss-Prot
Match: CUTA_HUMAN (Protein CutA OS=Homo sapiens GN=CUTA PE=1 SV=2)

HSP 1 Score: 93.5893 bits (231), Expect = 5.059e-19
Identity = 50/130 (38.46%), Postives = 82/130 (63.08%), Query Frame = 2

Query:  164 LCPTLSSSLKTGSAPSLPFFGSKFSNQARAKSSNTIKMEGNRNTVPSIVVYVTVPNKEAGKKLAESIVKAKLAACVNRVPGIESVYEWKGEIQTDAEELLIIKTRQSLLETLTEHVKANHEYDVPEVIAL 553
            L P +  ++ +GS P+ P         + A  S +  + G+ +       +VT PN++  K++A ++V+ +LAACVN +P I S+YEWKG+I+ D+E L++IKT+ SL+  LT+ V++ H Y+V EVIAL
Sbjct:   36 LLPRVLLTMASGSPPTQP---------SPASDSGSGYVPGSVSAA-----FVTCPNEKVAKEIARAVVEKRLAACVNLIPQITSIYEWKGKIEEDSEVLMMIKTQSSLVPALTDFVRSVHPYEVAEVIAL 151

HSP 2 Score: 22.3274 bits (46), Expect = 5.059e-19
Identity = 6/15 (40.00%), Postives = 12/15 (80.00%), Query Frame = 3

Query:  555 PITGGSQPYLEWLKK 599
            P+  G+ PYL+W+++
Sbjct:  152 PVEQGNFPYLQWVRQ 166

[Close]

BLAST of EY685444 vs. ExPASy Swiss-Prot
Match: CUTA_RAT (Protein CutA OS=Rattus norvegicus GN=Cuta PE=1 SV=2)

HSP 1 Score: 94.7449 bits (234), Expect = 5.063e-19
Identity = 52/130 (40.00%), Postives = 81/130 (62.31%), Query Frame = 2

Query:  164 LCPTLSSSLKTGSAPSLPFFGSKFSNQARAKSSNTIKMEGNRNTVPSIVVYVTVPNKEAGKKLAESIVKAKLAACVNRVPGIESVYEWKGEIQTDAEELLIIKTRQSLLETLTEHVKANHEYDVPEVIAL 553
            L P    S+ +GS PS P           + +S +  + G+ +       +VT PN++  K++A ++V+ +LAACVN +P I S+YEWKG+I+ D+E L++IKT+ SL+  LTE V++ H Y+V EVIAL
Sbjct:   36 LLPRALLSMASGSPPSQP-----------SPASGSGYVPGSVSAA-----FVTCPNEKVAKEIARAVVEKRLAACVNLIPQITSIYEWKGKIEEDSEVLMMIKTQSSLVPALTEFVRSVHPYEVAEVIAL 149

HSP 2 Score: 21.1718 bits (43), Expect = 5.063e-19
Identity = 6/13 (46.15%), Postives = 9/13 (69.23%), Query Frame = 3

Query:  555 PITGGSQPYLEWL 593
            P+  G+ PYL W+
Sbjct:  150 PVEQGNPPYLHWV 162

[Close]

BLAST of EY685444 vs. ExPASy Swiss-Prot
Match: CUTA_MOUSE (Protein CutA OS=Mus musculus GN=Cuta PE=2 SV=3)

HSP 1 Score: 93.9745 bits (232), Expect = 8.563e-19
Identity = 42/80 (52.50%), Postives = 63/80 (78.75%), Query Frame = 2

Query:  314 YVTVPNKEAGKKLAESIVKAKLAACVNRVPGIESVYEWKGEIQTDAEELLIIKTRQSLLETLTEHVKANHEYDVPEVIAL 553
            +VT PN++  K++A ++V+ +LAACVN +P I S+YEWKG+I+ D+E L++IKT+ SL+  LTE V++ H Y+V EVIAL
Sbjct:   70 FVTCPNEKVAKEIARAVVEKRLAACVNLIPQITSIYEWKGKIEEDSEVLMMIKTQSSLVPALTEFVRSVHPYEVAEVIAL 149

HSP 2 Score: 21.1718 bits (43), Expect = 8.563e-19
Identity = 6/13 (46.15%), Postives = 9/13 (69.23%), Query Frame = 3

Query:  555 PITGGSQPYLEWL 593
            P+  G+ PYL W+
Sbjct:  150 PVEQGNPPYLHWV 162

[Close]

BLAST of EY685444 vs. ExPASy Swiss-Prot
Match: CUTA_XENLA (Protein CutA homolog OS=Xenopus laevis GN=cuta PE=2 SV=2)

HSP 1 Score: 91.6633 bits (226), Expect = 2.473e-18
Identity = 43/80 (53.75%), Postives = 58/80 (72.50%), Query Frame = 2

Query:  314 YVTVPNKEAGKKLAESIVKAKLAACVNRVPGIESVYEWKGEIQTDAEELLIIKTRQSLLETLTEHVKANHEYDVPEVIAL 553
            YVT PN    K +A  +V+ KLAACVN +P I S+YEWKG+++ D E LL+IKTR S +  LTE+V++ H Y+V EVI+L
Sbjct:   53 YVTCPNDTVAKDIARGLVERKLAACVNVIPQITSIYEWKGKLEEDTEVLLMIKTRSSKVSALTEYVRSVHPYEVCEVISL 132

HSP 2 Score: 21.9422 bits (45), Expect = 2.473e-18
Identity = 7/13 (53.85%), Postives = 10/13 (76.92%), Query Frame = 3

Query:  555 PITGGSQPYLEWL 593
            PI  G+ PYL+W+
Sbjct:  133 PIEQGNPPYLKWV 145

[Close]

BLAST of EY685444 vs. ExPASy Swiss-Prot
Match: CUTA_BOVIN (Protein CutA OS=Bos taurus GN=CUTA PE=1 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 4.142e-18
Identity = 51/130 (39.23%), Postives = 80/130 (61.54%), Query Frame = 2

Query:  164 LCPTLSSSLKTGSAPSLPFFGSKFSNQARAKSSNTIKMEGNRNTVPSIVVYVTVPNKEAGKKLAESIVKAKLAACVNRVPGIESVYEWKGEIQTDAEELLIIKTRQSLLETLTEHVKANHEYDVPEVIAL 553
            L P    S+ +GS P+ P           + SS +  + G+ +       +VT PN++  K++A ++V+ +LA CVN VP I S+YEWKG+I+ D+E L++IKT+ SL+  LT+ V++ H Y+V EVIAL
Sbjct:   36 LLPRALLSMASGSPPAQP-----------SSSSRSAYVPGSVSAA-----FVTCPNEKVAKEIARAVVEKRLA-CVNLVPQITSIYEWKGKIEEDSEVLMMIKTQSSLVPALTDFVRSVHPYEVAEVIAL 148

HSP 2 Score: 23.483 bits (49), Expect = 4.142e-18
Identity = 6/15 (40.00%), Postives = 12/15 (80.00%), Query Frame = 3

Query:  555 PITGGSQPYLEWLKK 599
            P+  G+ PYL+W+++
Sbjct:  149 PVEQGNSPYLQWVRQ 163

[Close]

BLAST of EY685444 vs. ExPASy Swiss-Prot
Match: CUTA_THET8 (Divalent-cation tolerance protein cutA OS=Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) GN=cutA PE=1 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 1.243e-17
Identity = 40/82 (48.78%), Postives = 58/82 (70.73%), Query Frame = 2

Query:  308 VVYVTVPNKEAGKKLAESIVKAKLAACVNRVPGIESVYEWKGEIQTDAEELLIIKTRQSLLETLTEHVKANHEYDVPEVIAL 553
            VV +TVP++E  + +A+++V+ +LAACVN VPG+ S+Y W+GE+  D E LL++KT       L E VKA H Y VPE++AL
Sbjct:    4 VVLITVPSEEVARTIAKALVEERLAACVNIVPGLTSIYRWQGEVVEDQELLLLVKTTTHAFPKLKERVKALHPYTVPEIVAL 85

HSP 2 Score: 22.7126 bits (47), Expect = 1.243e-17
Identity = 7/15 (46.67%), Postives = 12/15 (80.00%), Query Frame = 3

Query:  555 PITGGSQPYLEWLKK 599
            PI  G++ YL+WL++
Sbjct:   86 PIAEGNREYLDWLRE 100

[Close]

BLAST of EY685444 vs. ExPASy Swiss-Prot
Match: CUTA_ERWCT (Divalent-cation tolerance protein cutA OS=Erwinia carotovora subsp. atroseptica GN=cutA PE=3 SV=2)

HSP 1 Score: 73.1738 bits (178), Expect = 4.494e-13
Identity = 31/90 (34.44%), Postives = 56/90 (62.22%), Query Frame = 2

Query:  284 NRNTVPSIVVYVTVPNKEAGKKLAESIVKAKLAACVNRVPGIESVYEWKGEIQTDAEELLIIKTRQSLLETLTEHVKANHEYDVPEVIAL 553
            +R    ++V+  T P+    ++LA S+++ +LAACV  +PG  S+Y W+G+++  +E  ++IK+  S  + L  H+K  H YD PE++ L
Sbjct:    3 DRPLCDAVVILCTAPDDACAQRLANSLLETRLAACVTLLPGARSLYYWEGKLEQQSEVQMLIKSDTSHQQALLTHLKQQHPYDTPELLVL 92

HSP 2 Score: 22.7126 bits (47), Expect = 4.494e-13
Identity = 7/13 (53.85%), Postives = 9/13 (69.23%), Query Frame = 3

Query:  555 PITGGSQPYLEWL 593
            P++GG   YL WL
Sbjct:   93 PVSGGDSDYLTWL 105

[Close]

BLAST of EY685444 vs. ExPASy Swiss-Prot
Match: CUTA_DANRE (Protein CutA homolog OS=Danio rerio GN=cuta PE=2 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 1.587e-12
Identity = 30/80 (37.50%), Postives = 52/80 (65.00%), Query Frame = 2

Query:  308 VVYVTVPNKEAGKKLAESIVKAKLAACVNRVPGIESVYEWKGEIQTDAEELLIIKTRQSLLETLTEHVKANHEYDVPEVI 547
            ++ V  P ++  + +   I++ +LAACVN  P   ++Y WKGEI+   E LL+++T+ SL++ L  ++ A H YD+PE+I
Sbjct:   49 LLLVNCPTEQTARDIGRIIMEKRLAACVNIFPRTATMYYWKGEIRDATEILLLVRTKTSLVQRLMTYITAIHPYDIPEII 128

HSP 2 Score: 23.8682 bits (50), Expect = 1.587e-12
Identity = 8/16 (50.00%), Postives = 12/16 (75.00%), Query Frame = 3

Query:  546 LPWPITGGSQPYLEWL 593
            + +PI  GSQ YL+W+
Sbjct:  128 ITFPINDGSQHYLKWI 143

The following BLAST results are available for this feature:

BLAST of EY685444 vs. ExPASy Swiss-Prot

BLAST of EY685444 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 36


Match Name	E-value	Identity	Description
CUTA_ARATH	9.957e-52	64.91	Protein CutA, chloroplastic OS=Arabidopsis thalian...	[more]
CUTA1_ORYSJ	4.982e-41	57.23	Protein CutA 1, chloroplastic OS=Oryza sativa subs...	[more]
CUTA_HUMAN	5.059e-19	38.46	Protein CutA OS=Homo sapiens GN=CUTA PE=1 SV=2	[more]
CUTA_RAT	5.063e-19	40.00	Protein CutA OS=Rattus norvegicus GN=Cuta PE=1 SV=...	[more]
CUTA_MOUSE	8.563e-19	52.50	Protein CutA OS=Mus musculus GN=Cuta PE=2 SV=3	[more]
CUTA_XENLA	2.473e-18	53.75	Protein CutA homolog OS=Xenopus laevis GN=cuta PE=...	[more]
CUTA_BOVIN	4.142e-18	39.23	Protein CutA OS=Bos taurus GN=CUTA PE=1 SV=1	[more]
CUTA_THET8	1.243e-17	48.78	Divalent-cation tolerance protein cutA OS=Thermus ...	[more]
CUTA_ERWCT	4.494e-13	34.44	Divalent-cation tolerance protein cutA OS=Erwinia ...	[more]
CUTA_DANRE	1.587e-12	37.50	Protein CutA homolog OS=Danio rerio GN=cuta PE=2 S...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C2-003-019-C12-CT.F Sweet orange bark, greenhouse plant Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY685444 ID=EY685444; Name=EY685444; organism=Citrus sinensis; type=EST; length=935bp
CCACGCGTCCGAAGTAAACCCCAAGTAATGAATCAGCAACTCACACTATA
AAATGGCTTCGACGCTCTGCACCAGAGCCTCGTCACTCGTTTCATCCCCC
ACAATACGACGTCGTCTGCCCTTAGTCGGGGCGTTTTGTATGCTAAGCTT
GGGCCTCACCAATCTCTGTCCCACTCTCTCTTCTTCCCTCAAAACGGGTT
CCGCTCCTTCTCTCCCGTTCTTCGGATCAAAGTTTAGTAACCAAGCTCGC
GCGAAGAGTAGTAACACGATCAAAATGGAAGGCAACAGAAACACTGTACC
AAGTATTGTAGTTTATGTCACCGTTCCTAACAAAGAAGCAGGAAAGAAAC
TGGCTGAAAGCATAGTGAAAGCAAAACTTGCAGCATGTGTGAATCGAGTG
CCAGGTATTGAATCAGTTTATGAGTGGAAGGGAGAGATCCAGACAGATGC
TGAAGAACTTCTTATTATAAAGACTAGGCAATCCCTTTTGGAAACTCTGA
CAGAGCATGTCAAGGCAAATCATGAATATGATGTGCCAGAAGTAATTGCC
TTGGCCCATCACTGGTGGGAGCCAGCCATACCTAGAATGGCTAAAAAAGC
AGTACAAAGGGGACTGACTCCCGCGGATGTACCAAGCGGAAGGAAGGGTG
GTGGAGCCTGCGAATGATAAGCTCCCTTTTGTTGGGGTGAGGCTCGCAAT
GAATACAACTAACTTGATAGACACTACACAGATCGCCATCACACGGACAC
CCTGGCCGGCATGGATGCAAGAGGCGTACGCGCGGCGGCCAAGGACCGAT
CCCCGGGGGCATACACACCAAAGTGAGTAGTATACGCCAGTCCGCGCACG
CCATGCCAACTGAACCCCACACGGAAAAGCCTCGAAGGGATACTCAGTAA
CGAGTACACGAACGCCGCACACAACCCACAGTGGC