EY685423

Overview
NameEY685423
Unique NameEY685423
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length957
Libraries
Library NameType
Sweet orange bark, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY685423 vs. ExPASy Swiss-Prot
Match: PIP1_SOLLC (Probable aquaporin PIP-type pTOM75 OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 7.897e-26
Identity = 69/128 (53.91%), Postives = 79/128 (61.72%), Query Frame = 2 
Query:  107 IQ*IACAFGDMIFTLVYCTADISG*HINPSETFRLLLPRNLSLTTATFYMVVHRLSCCMCTS*FVGILK*SNYKRLGGGANVAAPGYTTGDGLAAQIIGTVGGRGTPSTLPLDAKTNRKNSAVNYFGP 490
            IQ +A AFG MIF LVYCTA ISG HINP+ TF L L R LSLT A FYMV+  L   +C +  V       Y+RLGGGANV  PGYT GDGL A+IIGT     T  +   DAK N ++S V    P
Sbjct:   88 IQGVAWAFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRAVFYMVMQCLG-AICGAGVVKGFMVGPYQRLGGGANVVNPGYTKGDGLGAEIIGTFVLVYTVFS-ATDAKRNARDSHVPILAP 213

HSP 2 Score: 27.335 bits (59), Expect = 7.897e-26
Identity = 12/13 (92.31%), Postives = 12/13 (92.31%), Query Frame = 1 
Query:   19 FMATFLFLYITXL 57
            FMATFLFLYIT L
Sbjct:   59 FMATFLFLYITIL 71
[Close]
BLAST of EY685423 vs. ExPASy Swiss-Prot
Match: PIP12_ORYSJ (Probable aquaporin PIP1-2 OS=Oryza sativa subsp. japonica GN=PIP1-2 PE=2 SV=3)

HSP 1 Score: 108.227 bits (269), Expect = 8.462e-25
Identity = 67/128 (52.34%), Postives = 79/128 (61.72%), Query Frame = 2 
Query:  107 IQ*IACAFGDMIFTLVYCTADISG*HINPSETFRLLLPRNLSLTTATFYMVVHRLSCCMCTS*FVGILK*SNYKRLGGGANVAAPGYTTGDGLAAQIIGTVGGRGTPSTLPLDAKTNRKNSAVNYFGP 490
            IQ IA +FG MIF LVYCTA ISG HINP+ TF L L R LSLT A FYMV+  L   +C +  V   +   Y+  GGGANV APGYT GDGL A+I+GT     T  +   DAK N ++S V    P
Sbjct:   89 IQGIAWSFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRALFYMVMQCLG-AICGAGVVKGFQKGLYETTGGGANVVAPGYTKGDGLGAEIVGTFILVYTVFS-ATDAKRNARDSHVPILAP 214

HSP 2 Score: 27.335 bits (59), Expect = 8.462e-25
Identity = 12/13 (92.31%), Postives = 12/13 (92.31%), Query Frame = 1 
Query:   19 FMATFLFLYITXL 57
            FMATFLFLYIT L
Sbjct:   60 FMATFLFLYITVL 72
[Close]
BLAST of EY685423 vs. ExPASy Swiss-Prot
Match: PIP16_MAIZE (Aquaporin PIP1-6 OS=Zea mays GN=PIP1-6 PE=2 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 3.286e-23
Identity = 55/100 (55.00%), Postives = 68/100 (68.00%), Query Frame = 2 
Query:  107 IQ*IACAFGDMIFTLVYCTADISG*HINPSETFRLLLPRNLSLTTATFYMVVHRLSCCMCTS*FVGILK*SNYKRLGGGANVAAPGYTTGDGLAAQIIGT 406
            IQ IA AFG MIF LVYCTA +SG HINP+ TF LLL R LSLT A +Y+V+  L   +C +  V     + Y+  GGGAN  +PGYT GDGL A+++GT
Sbjct:   95 IQGIAWAFGGMIFALVYCTAGVSGGHINPAVTFGLLLARKLSLTRAVYYVVMQCLG-AVCGAGVVKAFGSALYESAGGGANAVSPGYTKGDGLGAEVVGT 193

HSP 2 Score: 25.409 bits (54), Expect = 3.286e-23
Identity = 10/13 (76.92%), Postives = 12/13 (92.31%), Query Frame = 1 
Query:   19 FMATFLFLYITXL 57
            F+ATFLFLY+T L
Sbjct:   66 FVATFLFLYVTVL 78

HSP 3 Score: 21.1718 bits (43), Expect = 3.286e-23
Identity = 13/41 (31.71%), Postives = 19/41 (46.34%), Query Frame = 3 
Query:  450 RQTAKTLLSTTLAPPLICITSYHEHTSQPPHNNTGIRKAKS 572
            ++TA+      LAP  I    +  H +  P   TGI  A+S
Sbjct:  207 KRTARDSHVPALAPLPIGFAVFLVHLATIPITGTGINPARS 247
[Close]
BLAST of EY685423 vs. ExPASy Swiss-Prot
Match: PIP13_MAIZE (Aquaporin PIP1-3/PIP1-4 OS=Zea mays GN=PIP1-3 PE=2 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 3.380e-23
Identity = 65/128 (50.78%), Postives = 78/128 (60.94%), Query Frame = 2 
Query:  107 IQ*IACAFGDMIFTLVYCTADISG*HINPSETFRLLLPRNLSLTTATFYMVVHRLSCCMCTS*FVGILK*SNYKRLGGGANVAAPGYTTGDGLAAQIIGTVGGRGTPSTLPLDAKTNRKNSAVNYFGP 490
            IQ IA +FG MIF LVYCTA ISG HINP+ TF L L R LSLT A FY+++  L   +C +  V   +   Y   GGGANV APGYT GDGL A+I+GT     T  +   DAK N ++S V    P
Sbjct:   93 IQGIAWSFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRAIFYIIMQCLG-AICGAGVVKGFQQGLYMGNGGGANVVAPGYTKGDGLGAEIVGTFILVYTVFS-ATDAKRNARDSHVPILAP 218

HSP 2 Score: 25.7942 bits (55), Expect = 3.380e-23
Identity = 11/13 (84.62%), Postives = 12/13 (92.31%), Query Frame = 1 
Query:   19 FMATFLFLYITXL 57
            F+ATFLFLYIT L
Sbjct:   64 FVATFLFLYITVL 76
[Close]
BLAST of EY685423 vs. ExPASy Swiss-Prot
Match: PIP12_MAIZE (Aquaporin PIP1-2 OS=Zea mays GN=PIP1-2 PE=1 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 3.380e-23
Identity = 65/128 (50.78%), Postives = 78/128 (60.94%), Query Frame = 2 
Query:  107 IQ*IACAFGDMIFTLVYCTADISG*HINPSETFRLLLPRNLSLTTATFYMVVHRLSCCMCTS*FVGILK*SNYKRLGGGANVAAPGYTTGDGLAAQIIGTVGGRGTPSTLPLDAKTNRKNSAVNYFGP 490
            IQ IA +FG MIF LVYCTA ISG HINP+ TF L L R LSLT A FY+++  L   +C +  V   +   Y   GGGANV APGYT GDGL A+I+GT     T  +   DAK N ++S V    P
Sbjct:   90 IQGIAWSFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRALFYIIMQCLG-AVCGAGVVKGFQQGLYMGNGGGANVVAPGYTKGDGLGAEIVGTFILVYTVFS-ATDAKRNARDSHVPILAP 215

HSP 2 Score: 25.7942 bits (55), Expect = 3.380e-23
Identity = 11/13 (84.62%), Postives = 12/13 (92.31%), Query Frame = 1 
Query:   19 FMATFLFLYITXL 57
            F+ATFLFLYIT L
Sbjct:   61 FVATFLFLYITIL 73
[Close]
BLAST of EY685423 vs. ExPASy Swiss-Prot
Match: PIP14_ARATH (Probable aquaporin PIP1-4 OS=Arabidopsis thaliana GN=PIP1.4 PE=1 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 5.725e-23
Identity = 65/128 (50.78%), Postives = 78/128 (60.94%), Query Frame = 2 
Query:  107 IQ*IACAFGDMIFTLVYCTADISG*HINPSETFRLLLPRNLSLTTATFYMVVHRLSCCMCTS*FVGILK*SNYKRLGGGANVAAPGYTTGDGLAAQIIGTVGGRGTPSTLPLDAKTNRKNSAVNYFGP 490
            IQ IA AFG MIF LVYCTA ISG HINP+ TF L L R LSLT A FYM++  L   +C +  V   + + Y+ LGGGAN  A GYT G GL A+IIGT     T  +   DAK + ++S V    P
Sbjct:   88 IQGIAWAFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRAVFYMIMQCLG-AICGAGVVKGFQPTPYQTLGGGANTVAHGYTKGSGLGAEIIGTFVLVYTVFS-ATDAKRSARDSHVPILAP 213

HSP 2 Score: 25.7942 bits (55), Expect = 5.725e-23
Identity = 11/13 (84.62%), Postives = 12/13 (92.31%), Query Frame = 1 
Query:   19 FMATFLFLYITXL 57
            F+ATFLFLYIT L
Sbjct:   59 FIATFLFLYITVL 71
[Close]
BLAST of EY685423 vs. ExPASy Swiss-Prot
Match: PIP12_ARATH (Aquaporin PIP1-2 OS=Arabidopsis thaliana GN=PIP1-2 PE=1 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 7.449e-23
Identity = 65/128 (50.78%), Postives = 77/128 (60.16%), Query Frame = 2 
Query:  107 IQ*IACAFGDMIFTLVYCTADISG*HINPSETFRLLLPRNLSLTTATFYMVVHRLSCCMCTS*FVGILK*SNYKRLGGGANVAAPGYTTGDGLAAQIIGTVGGRGTPSTLPLDAKTNRKNSAVNYFGP 490
            IQ IA AFG MIF LVYCTA ISG HINP+ TF L L R LSLT A +Y+V+  L   +C +  V   +   Y+ LGGGAN  A GYT G GL A+IIGT     T  +   DAK N ++S V    P
Sbjct:   87 IQGIAWAFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRAVYYIVMQCLG-AICGAGVVKGFQPKQYQALGGGANTIAHGYTKGSGLGAEIIGTFVLVYTVFS-ATDAKRNARDSHVPILAP 212

HSP 2 Score: 25.7942 bits (55), Expect = 7.449e-23
Identity = 11/13 (84.62%), Postives = 12/13 (92.31%), Query Frame = 1 
Query:   19 FMATFLFLYITXL 57
            F+ATFLFLYIT L
Sbjct:   58 FIATFLFLYITVL 70
[Close]
BLAST of EY685423 vs. ExPASy Swiss-Prot
Match: PIP11_VICFA (Aquaporin PIP1.1 OS=Vicia faba GN=PIP1.1 PE=2 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 7.449e-23
Identity = 65/128 (50.78%), Postives = 77/128 (60.16%), Query Frame = 2 
Query:  107 IQ*IACAFGDMIFTLVYCTADISG*HINPSETFRLLLPRNLSLTTATFYMVVHRLSCCMCTS*FVGILK*SNYKRLGGGANVAAPGYTTGDGLAAQIIGTVGGRGTPSTLPLDAKTNRKNSAVNYFGP 490
            IQ IA AFG MIF LVYCTA ISG HINP+ TF L L R LSLT A +Y+V+  L   +C +  V   +   Y+ LGGGAN  A GYT G GL A+IIGT     T  +   DAK N ++S V    P
Sbjct:   87 IQGIAWAFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRALYYIVMQCLG-AICGAGVVKGFQPKQYQALGGGANTVAHGYTKGSGLGAEIIGTFVLVYTVFS-ATDAKRNARDSHVPILAP 212

HSP 2 Score: 25.7942 bits (55), Expect = 7.449e-23
Identity = 11/13 (84.62%), Postives = 12/13 (92.31%), Query Frame = 1 
Query:   19 FMATFLFLYITXL 57
            F+ATFLFLYIT L
Sbjct:   58 FIATFLFLYITVL 70
[Close]
BLAST of EY685423 vs. ExPASy Swiss-Prot
Match: PIP11_ARATH (Aquaporin PIP1-1 OS=Arabidopsis thaliana GN=PIP1-1 PE=1 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 7.449e-23
Identity = 65/128 (50.78%), Postives = 77/128 (60.16%), Query Frame = 2 
Query:  107 IQ*IACAFGDMIFTLVYCTADISG*HINPSETFRLLLPRNLSLTTATFYMVVHRLSCCMCTS*FVGILK*SNYKRLGGGANVAAPGYTTGDGLAAQIIGTVGGRGTPSTLPLDAKTNRKNSAVNYFGP 490
            IQ IA AFG MIF LVYCTA ISG HINP+ TF L L R LSLT A +Y+V+  L   +C +  V   +   Y+ LGGGAN  A GYT G GL A+IIGT     T  +   DAK N ++S V    P
Sbjct:   87 IQGIAWAFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRALYYIVMQCLG-AICGAGVVKGFQPKQYQALGGGANTVAHGYTKGSGLGAEIIGTFVLVYTVFS-ATDAKRNARDSHVPILAP 212

HSP 2 Score: 25.7942 bits (55), Expect = 7.449e-23
Identity = 11/13 (84.62%), Postives = 12/13 (92.31%), Query Frame = 1 
Query:   19 FMATFLFLYITXL 57
            F+ATFLFLYIT L
Sbjct:   58 FIATFLFLYITVL 70
[Close]
BLAST of EY685423 vs. ExPASy Swiss-Prot
Match: PIP2_PEA (Probable aquaporin PIP-type 7a OS=Pisum sativum GN=TRG-31 PE=2 SV=2)

HSP 1 Score: 102.449 bits (254), Expect = 1.261e-22
Identity = 57/100 (57.00%), Postives = 62/100 (62.00%), Query Frame = 2 
Query:  107 IQ*IACAFGDMIFTLVYCTADISG*HINPSETFRLLLPRNLSLTTATFYMVVHRLSCCMCTS*FVGILK*SNYKRLGGGANVAAPGYTTGDGLAAQIIGT 406
            IQ IA AFG MIF LVYCTA ISG HINP+ TF L L R LSLT A FYMV+  L          G      +  L GGAN  APGYT GDGL A+I+GT
Sbjct:   90 IQGIAWAFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRAIFYMVMQVLGAICGAGVVKGFEGKQRFGDLNGGANFVAPGYTKGDGLGAEIVGT 189

HSP 2 Score: 25.7942 bits (55), Expect = 1.261e-22
Identity = 11/13 (84.62%), Postives = 12/13 (92.31%), Query Frame = 1 
Query:   19 FMATFLFLYITXL 57
            F+ATFLFLYIT L
Sbjct:   61 FIATFLFLYITVL 73
The following BLAST results are available for this feature:
BLAST of EY685423 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 40
Match NameE-valueIdentityDescription
PIP1_SOLLC7.897e-2653.91Probable aquaporin PIP-type pTOM75 OS=Solanum lyco... [more]
PIP12_ORYSJ8.462e-2552.34Probable aquaporin PIP1-2 OS=Oryza sativa subsp. j... [more]
PIP16_MAIZE3.286e-2355.00Aquaporin PIP1-6 OS=Zea mays GN=PIP1-6 PE=2 SV=1[more]
PIP13_MAIZE3.380e-2350.78Aquaporin PIP1-3/PIP1-4 OS=Zea mays GN=PIP1-3 PE=2... [more]
PIP12_MAIZE3.380e-2350.78Aquaporin PIP1-2 OS=Zea mays GN=PIP1-2 PE=1 SV=1[more]
PIP14_ARATH5.725e-2350.78Probable aquaporin PIP1-4 OS=Arabidopsis thaliana ... [more]
PIP12_ARATH7.449e-2350.78Aquaporin PIP1-2 OS=Arabidopsis thaliana GN=PIP1-2... [more]
PIP11_VICFA7.449e-2350.78Aquaporin PIP1.1 OS=Vicia faba GN=PIP1.1 PE=2 SV=1[more]
PIP11_ARATH7.449e-2350.78Aquaporin PIP1-1 OS=Arabidopsis thaliana GN=PIP1-1... [more]
PIP2_PEA1.261e-2257.00Probable aquaporin PIP-type 7a OS=Pisum sativum GN... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C2-003-019-A10-CT.F Sweet orange bark, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY685423 ID=EY685423; Name=EY685423; organism=Citrus sinensis; type=EST; length=957bp
CCACGCTTCCGTGCATAGTTCATGGCTACCTTCTTGTTCCTCTACATCAC
TNGCCTTGACTGTCATGAGTGTCTCTAATTCTAACACCAACTGCTCCACT
GTACTCATCCAATGAATTGCTTGTGCCTTTGGTGACATGATCTTTACGCT
TGTTTACTGCACTGCTGATATCTCAGGATGACACATCAACCCATCAGAGA
CCTTCCGTCTGCTGCTACCTAGGAATCTTTCCCTGACAACGGCTACCTTC
TACATGGTCGTGCACCGCCTTAGTTGCTGCATGTGCACCTCCTAATTTGT
AGGGATTCTCAAGTGAAGTAACTACAAAAGGTTGGGTGGTGGAGCCAATG
TTGCTGCTCCCGGCTACACCACGGGTGATGGCCTTGCTGCTCAAATAATT
GGCACCGTGGGGGGGCGGGGTACACCGTCCACACTGCCACTCGATGCGAA
GACAAACCGCAAAAACTCTGCTGTCAACTACTTTGGCCCCACCTCTCATC
TGTATTACCTCTTATCACGAACACACAAGCCAACCGCCGCATAATAACAC
CGGCATACGCAAGGCCAAATCGGACTGGAACCACTCTCACCAATAACTAC
CACCCAAAGCCTCCATCACCTCACCTCTCACTAACACCCCCACCTCCACT
ATGAACACAACGACACACCAGAAAAACAGAACAAAAACACCAGGAAGAAT
GCCAATAATGCTCCGACACACAAAAGGCCAACCACGCATACACAAGGACG
CAGTCCCAAACCGCCACACCAACCCGACAGAACACACAAACCACTACCAA
CCGATCACAACATACTCAGATGACACCCATACACACAGAGCCTCCAGAAA
CTCCTCCAACAACGCAACTCAACCCACAATCAACACACACCATCCCATAC
AGCCAACAAATACGCAGCAAACCAAGAACATAATCACGCAGAAAACCTAA
CCACCCA
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