EY684724

Overview
NameEY684724
Unique NameEY684724
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length848
Libraries
Library NameType
Sweet orange bark, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
EY684724_ssr197 microsatellite EY684724_ssr197:197..211. BLAST: Citrus ESTs to Prunus persica proteins V1
Homology
[Close]
BLAST of EY684724 vs. ExPASy Swiss-Prot
Match: CSN8_ARATH (COP9 signalosome complex subunit 8 OS=Arabidopsis thaliana GN=CSN8 PE=1 SV=1)

HSP 1 Score: 189.119 bits (479), Expect = 1.112e-57
Identity = 87/119 (73.11%), Postives = 103/119 (86.55%), Query Frame = 2 
Query:  227 QVAAEGIVFQDEWPYVIHLLGYYYVNDINSARFLWKSIPSTIKDSRAEVVAAWKIGQHLWTRDYAGVYDAIRGFDWSQEAQALVAAFSELYTKRMFQLLMSAYSTISTQDTALFLGMNE 583
            QVA+EGI + D+WPY IHLLGY+YV+D +SARFLWK IP+ IK+ + EVVAAW IGQ LWT DYAGVY+AIRG+DWSQEA+ +VAAFS+LYTKRMFQLL+SAYSTI+  D ALFLGM E
Sbjct:   28 QVASEGIEYHDDWPYAIHLLGYFYVDDCDSARFLWKRIPTAIKERKPEVVAAWGIGQKLWTHDYAGVYEAIRGYDWSQEAKDMVAAFSDLYTKRMFQLLLSAYSTITIHDLALFLGMTE 146

HSP 2 Score: 45.4394 bits (106), Expect = 1.112e-57
Identity = 20/29 (68.97%), Postives = 26/29 (89.66%), Query Frame = 3 
Query:   51 MDFSPLTDALASKSYEKIADICDDLMLKV 137
            MD SP+ +ALA+KS++KIADICD LML+V
Sbjct:    1 MDLSPVKEALAAKSFDKIADICDTLMLQV 29

HSP 3 Score: 31.187 bits (69), Expect = 1.112e-57
Identity = 13/28 (46.43%), Postives = 18/28 (64.29%), Query Frame = 3 
Query:  582 KDDAAYYVQQQGWNLDPASRMVDGEKTA 665
            +DDA  YV + GW +D AS+M   +K A
Sbjct:  146 EDDATTYVVENGWTVDAASQMASVKKQA 173

HSP 4 Score: 21.1718 bits (43), Expect = 1.112e-57
Identity = 10/21 (47.62%), Postives = 14/21 (66.67%), Query Frame = 1 
Query:  655 KKQPLVTEQKLKSPKMPCLTK 717
            KKQ +  EQK+ S K+  LT+
Sbjct:  170 KKQAVKREQKVDSSKLQRLTE 190
[Close]
BLAST of EY684724 vs. ExPASy Swiss-Prot
Match: CSN8_XENLA (COP9 signalosome complex subunit 8 OS=Xenopus laevis GN=csn8 PE=2 SV=2)

HSP 1 Score: 95.1301 bits (235), Expect = 5.167e-19
Identity = 40/96 (41.67%), Postives = 62/96 (64.58%), Query Frame = 2 
Query:  290 YYYVNDINSARFLWKSIPSTIKDSRAEVVAAWKIGQHLWTRDYAGVYDAIRGFDWSQEAQALVAAFSELYTKRMFQLLMSAYSTISTQDTALFLGM 577
            Y   ND+N+AR+LWK IPS IK S +E+   W++GQ +W RD+ G+Y +I  + WS+  Q ++ A  +   +R F L+  AY++IS  D A F+G+
Sbjct:   46 YLLHNDLNNARYLWKRIPSAIKSSHSELGGIWEVGQKIWQRDFPGIYTSISAYQWSENIQQIMEAVRDATQQRAFGLVSQAYTSISADDFAAFVGL 141
[Close]
BLAST of EY684724 vs. ExPASy Swiss-Prot
Match: CSN8_DANRE (COP9 signalosome complex subunit 8 OS=Danio rerio GN=cops8 PE=2 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 6.002e-19
Identity = 40/98 (40.82%), Postives = 61/98 (62.24%), Query Frame = 2 
Query:  281 LLGYYYVNDINSARFLWKSIPSTIKDSRAEVVAAWKIGQHLWTRDYAGVYDAIRGFDWSQEAQALVAAFSELYTKRMFQLLMSAYSTISTQDTALFLG 574
            L+ Y   ND+N+AR+LWK IP  IK +  E+ A W +GQ +W RD+ G+Y AI  + WS+    ++ A  E   +R + L+  AY++IS +D A F+G
Sbjct:   39 LVLYLLHNDMNNARYLWKRIPQAIKTANPEMAAIWAVGQRIWQRDFPGIYSAIAAYQWSESILPVMEALRESTRRRAYGLVAQAYTSISAEDFAAFVG 136

HSP 2 Score: 23.0978 bits (48), Expect = 6.002e-19
Identity = 9/25 (36.00%), Postives = 14/25 (56.00%), Query Frame = 3 
Query:  585 DDAAYYVQQQGWNLDPASRMVDGEK 659
            ++A   V   GW  DP +RM+  +K
Sbjct:  140 EEAVKGVVSHGWQADPNTRMIMPQK 164
[Close]
BLAST of EY684724 vs. ExPASy Swiss-Prot
Match: CSN8_MOUSE (COP9 signalosome complex subunit 8 OS=Mus musculus GN=Cops8 PE=1 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 1.012e-18
Identity = 37/96 (38.54%), Postives = 57/96 (59.38%), Query Frame = 2 
Query:  290 YYYVNDINSARFLWKSIPSTIKDSRAEVVAAWKIGQHLWTRDYAGVYDAIRGFDWSQEAQALVAAFSELYTKRMFQLLMSAYSTISTQDTALFLGM 577
            Y   ND+N+AR+LWK IP  IK + +E+   W +GQ +W RD+ G+Y  I    WS+  Q ++ A  +   +R F L+  AY++I   D A F+G+
Sbjct:   44 YLLQNDMNNARYLWKRIPPAIKSANSELGGIWSVGQRIWQRDFPGIYTTINAHQWSETVQPIMEALRDATRRRAFALVSQAYTSIIADDFAAFVGL 139

HSP 2 Score: 25.409 bits (54), Expect = 1.012e-18
Identity = 12/28 (42.86%), Postives = 16/28 (57.14%), Query Frame = 3 
Query:  585 DDAAYYVQQQGWNLDPASRMVDGEKTAS 668
            ++A   V +QGW  D  +RMV   K AS
Sbjct:  142 EEAVKGVLEQGWQADSTTRMVLPRKPAS 169
[Close]
BLAST of EY684724 vs. ExPASy Swiss-Prot
Match: CSN8_XENTR (COP9 signalosome complex subunit 8 OS=Xenopus tropicalis GN=csn8 PE=2 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 1.717e-18
Identity = 38/97 (39.18%), Postives = 61/97 (62.89%), Query Frame = 2 
Query:  290 YYYVNDINSARFLWKSIPSTIKDSRAEVVAAWKIGQHLWTRDYAGVYDAIRGFDWSQEAQALVAAFSELYTKRMFQLLMSAYSTISTQDTALFLGMN 580
            Y   ND+N+AR+LWK IP  IK + +E+   W++GQ +W RD+ G+Y +I    WS+  Q ++ A  +   +R F L+  AY++IS  D A F+G++
Sbjct:   44 YLLHNDMNNARYLWKRIPPAIKSAHSELGGIWEVGQKIWQRDFPGIYTSISACQWSEAIQPVMEAVRDATRRRAFGLVSQAYTSISADDFAAFVGLS 140

HSP 2 Score: 24.2534 bits (51), Expect = 1.717e-18
Identity = 12/28 (42.86%), Postives = 17/28 (60.71%), Query Frame = 3 
Query:  585 DDAAYYVQQQGWNLDPASRMVDGEKTAS 668
            ++A   V +QGW  D A+RMV  +K  S
Sbjct:  142 EEAVKGVIEQGWQADLATRMVMPKKPDS 169
[Close]
BLAST of EY684724 vs. ExPASy Swiss-Prot
Match: CSN8_RAT (COP9 signalosome complex subunit 8 OS=Rattus norvegicus GN=Cops8 PE=2 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 2.893e-18
Identity = 37/96 (38.54%), Postives = 57/96 (59.38%), Query Frame = 2 
Query:  290 YYYVNDINSARFLWKSIPSTIKDSRAEVVAAWKIGQHLWTRDYAGVYDAIRGFDWSQEAQALVAAFSELYTKRMFQLLMSAYSTISTQDTALFLGM 577
            Y   ND+N+AR+LWK IP  IK + +E+   W +GQ +W RD+ G+Y  I    WS+  Q ++ A  +   +R F L+  AY++I   D A F+G+
Sbjct:   44 YLLQNDMNNARYLWKRIPPAIKSANSELGGIWSVGQRIWQRDFPGIYTTINAHQWSETVQPIMEALRDATRRRAFALVSQAYTSIIADDFAAFVGL 139

HSP 2 Score: 23.8682 bits (50), Expect = 2.893e-18
Identity = 11/27 (40.74%), Postives = 15/27 (55.56%), Query Frame = 3 
Query:  585 DDAAYYVQQQGWNLDPASRMVDGEKTA 665
            ++A   V +QGW  D  +RMV   K A
Sbjct:  142 EEAVKGVLEQGWQADSTTRMVLPRKPA 168
[Close]
BLAST of EY684724 vs. ExPASy Swiss-Prot
Match: CSN8_PONAB (COP9 signalosome complex subunit 8 OS=Pongo abelii GN=COPS8 PE=2 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 6.362e-18
Identity = 40/105 (38.10%), Postives = 61/105 (58.10%), Query Frame = 2 
Query:  266 PYVIHLLGYYYV-NDINSARFLWKSIPSTIKDSRAEVVAAWKIGQHLWTRDYAGVYDAIRGFDWSQEAQALVAAFSELYTKRMFQLLMSAYSTISTQDTALFLGM 577
            P    LL  Y + ND+N+AR+LWK IP  IK + +E+   W +GQ +W RD+ G+Y  I    WS+  Q ++ A  +   +R F L+  AY++I   D A F+G+
Sbjct:   35 PVYGQLLALYLLHNDMNNARYLWKRIPPAIKSANSELGGIWSVGQRIWQRDFPGIYTTINAHQWSETVQPIMEALRDATRRRAFALVSQAYTSIVADDFAAFVGL 139

HSP 2 Score: 22.7126 bits (47), Expect = 6.362e-18
Identity = 8/21 (38.10%), Postives = 13/21 (61.90%), Query Frame = 3 
Query:  585 DDAAYYVQQQGWNLDPASRMV 647
            ++A   + +QGW  D  +RMV
Sbjct:  142 EEAVKGILEQGWQADSTTRMV 162
[Close]
BLAST of EY684724 vs. ExPASy Swiss-Prot
Match: CSN8_HUMAN (COP9 signalosome complex subunit 8 OS=Homo sapiens GN=COPS8 PE=1 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 6.362e-18
Identity = 40/105 (38.10%), Postives = 61/105 (58.10%), Query Frame = 2 
Query:  266 PYVIHLLGYYYV-NDINSARFLWKSIPSTIKDSRAEVVAAWKIGQHLWTRDYAGVYDAIRGFDWSQEAQALVAAFSELYTKRMFQLLMSAYSTISTQDTALFLGM 577
            P    LL  Y + ND+N+AR+LWK IP  IK + +E+   W +GQ +W RD+ G+Y  I    WS+  Q ++ A  +   +R F L+  AY++I   D A F+G+
Sbjct:   35 PVYGQLLALYLLHNDMNNARYLWKRIPPAIKSANSELGGIWSVGQRIWQRDFPGIYTTINAHQWSETVQPIMEALRDATRRRAFALVSQAYTSIIADDFAAFVGL 139

HSP 2 Score: 22.7126 bits (47), Expect = 6.362e-18
Identity = 8/21 (38.10%), Postives = 13/21 (61.90%), Query Frame = 3 
Query:  585 DDAAYYVQQQGWNLDPASRMV 647
            ++A   + +QGW  D  +RMV
Sbjct:  142 EEAVKGILEQGWQADSTTRMV 162
The following BLAST results are available for this feature:
BLAST of EY684724 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 8
Match NameE-valueIdentityDescription
CSN8_ARATH1.112e-5773.11COP9 signalosome complex subunit 8 OS=Arabidopsis ... [more]
CSN8_XENLA5.167e-1941.67COP9 signalosome complex subunit 8 OS=Xenopus laev... [more]
CSN8_DANRE6.002e-1940.82COP9 signalosome complex subunit 8 OS=Danio rerio ... [more]
CSN8_MOUSE1.012e-1838.54COP9 signalosome complex subunit 8 OS=Mus musculus... [more]
CSN8_XENTR1.717e-1839.18COP9 signalosome complex subunit 8 OS=Xenopus trop... [more]
CSN8_RAT2.893e-1838.54COP9 signalosome complex subunit 8 OS=Rattus norve... [more]
CSN8_PONAB6.362e-1838.10COP9 signalosome complex subunit 8 OS=Pongo abelii... [more]
CSN8_HUMAN6.362e-1838.10COP9 signalosome complex subunit 8 OS=Homo sapiens... [more]
back to top
Properties
Property NameValue
Genbank descriptionCS00-C2-003-002-G08-CT.F Sweet orange bark, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY684724 ID=EY684724; Name=EY684724; organism=Citrus sinensis; type=EST; length=848bp
AAAATTTTACAGAGTAAGAATAAGAGCAGGGAAGAAGAGTAGGGAGTGCG
ATGGACTTCTCTCCTTTAACAGACGCTTTAGCGTCCAAATCGTATGAAAA
AATCGCTGATATTTGCGACGACCTAATGCTCAAGGTTAATTTTTTTTTTC
CTTTTTAATAATTAATCTTATTTTCAAAAATTAATTTCTTCATCTCATTA
TTATTATTATTAAAATTTCTCTTTAACAGGTTGCTGCAGAGGGTATTGTG
TTTCAAGACGAATGGCCTTACGTGATTCATCTCCTCGGTTACTATTATGT
CAATGATATTAACAGTGCACGCTTCCTTTGGAAATCAATACCTTCGACAA
TTAAGGATAGCAGGGCAGAAGTGGTTGCAGCTTGGAAAATTGGTCAGCAT
CTATGGACACGGGATTATGCTGGTGTTTATGACGCCATTCGTGGGTTTGA
TTGGAGTCAAGAAGCACAAGCTCTTGTTGCTGCTTTCTCAGAGCTCTACA
CCAAGAGGATGTTTCAGCTGCTTATGTCTGCTTATTCGACAATAAGCACC
CAAGACACAGCTCTCTTTCTGGGAATGAATGAAGGATGATGCCGCATATT
ACGTACAACAGCAAGGTTGGAATTTGGATCCTGCTTCTCGAATGGTTGAC
GGGGAAAAAACAGCCTCTTGTGACAGAGCAAAAACTAAAATCCCCCAAAA
TGCCGTGCTTGACCAAAAATGGTGTTCCACCTTGGACATTGATTTTACCC
TCTTGAACGGGCCAAAACTGGCAATTCTCCTGGGGAAACCACTTTTTGAT
CCCGGAACAGAAGTGGCTTCCAAACGTTTTAAAACCCCGGATTTTTCG
back to top