Degreening behavior in ‘Fallglo' and ‘Lee×Orlando' is correlated with differential expression of ethylene signaling and biosynthesis genes

Publication Overview
TitleDegreening behavior in ‘Fallglo' and ‘Lee×Orlando' is correlated with differential expression of ethylene signaling and biosynthesis genes
AuthorsJohn-Karuppiah K, Burns JK
TypeJournal Article
Journal NamePostharvest biology and technology
Volume58
Issue3
Year2010
Page(s)185-193
CitationJohn-Karuppiah K, Burns JK. Degreening behavior in ‘Fallglo' and ‘Lee×Orlando' is correlated with differential expression of ethylene signaling and biosynthesis genes. Postharvest biology and technology. 2010; 58(3):185-193.

Abstract

Two citrus types (‘Fallglo' and ‘Lee×Orlando') exhibiting differential fruit degreening response when treated with ethylene were selected. Fruit were harvested at commercial maturity but at different developmental periods (Harvest I, II and III). Rate of color change was greater in ‘Fallglo' than in ‘Lee×Orlando' when fruit were treated with 5μLL⁻¹ of ethylene for 24h. After 24h of transfer of fruit to ethylene-free storage, rate of change decreased in ‘Fallgo' and exhibited varied response in ‘Lee×Orlando' depending on harvest date. ‘Fallglo' fruit from Harvests I and II were completely degreened at the end of storage for 7d; however ‘Lee×Orlando' were not and were green in color. No difference in seedling triple response was observed between ‘Fallglo' and ‘Lee×Orlando' and sequences of the four ethylene receptors were identical between them. Expression of genes involved in ethylene biosynthesis and signaling pathways were studied in flavedo to test if differences in these pathways were correlated with differential ethylene sensitivity of the citrus types. Basal levels of ACS2 and ACO expressions declined as maturity progressed, and ethylene-induced expression of ACS1 and ACO were influenced by fruit maturity. At Harvests I and II, ethylene-induced increase in ACS1 and ACO expressions and ACC levels were greater in ‘Fallglo' than in ‘Lee×Orlando'. Ethylene treatment influenced MACC content only during Harvest I in ‘Lee×Orlando'. MACC levels were generally higher in ‘Lee×Orlando' than in ‘Fallglo'. Expressions of ETR1 and ETR2 were ethylene responsive in ‘Fallglo' and only ETR1 expression was ethylene responsive in ‘Lee×Orlando'. Ethylene had more impact on ETR1 expression in ‘Fallglo' than in ‘Lee×Orlando'. Ethylene had a negative effect on ETR3 expression which was more pronounced in ‘Lee×Orlando' than in ‘Fallglo'. Expressions of ERS1, CTR1, EIN2, EIL1 and EIL2 were not affected by ethylene in both citrus types. Expression of chlorophyllase gene and rate of total chlorophyll degradation were higher in ‘Fallglo' than in ‘Lee×Orlando' during ethylene treatment. Differential degreening behavior of ‘Fallglo' and ‘Lee×Orlando' correlated with peel maturity, and factor(s) downstream of ethylene signaling but upstream of ethylene biosynthesis play a role in the differential sensitivity.
Features
This publication contains information about 17 features:
Feature NameUniquenameType
ACO.1ACO.1genetic_marker
ACS1.1ACS1.1genetic_marker
ACS2.1ACS2.1genetic_marker
CHLCHLgenetic_marker
CTR1CTR1genetic_marker
EIL1EIL1genetic_marker
EIL2EIL2genetic_marker
EIN2EIN2genetic_marker
ERS1-fullERS1-fullgenetic_marker
ERS1-partialERS1-partialgenetic_marker
ETR1- fullETR1- fullgenetic_marker
ETR1-partialETR1-partialgenetic_marker
ETR2-fullETR2-fullgenetic_marker
ETR2-partialETR2-partialgenetic_marker
ETR3-fullETR3-fullgenetic_marker
ETR3-partialETR3-partialgenetic_marker
GAPDHGAPDHgenetic_marker
Stocks
This publication contains information about 14 stocks:
Stock NameUniquenameType
BlancoBlancocultivar
DancyDancycultivar
DuncanDuncancultivar
LeeLeecultivar
OrlandoOrlandocultivar
ClementineClementinecultivar
Dancy_x_DuncanDancy_x_Duncanpopulation
Lee_×_OrlandoLee_×_Orlandopopulation
DxD_x_ClementineDxD_x_Clementinepopulation
Blanco_X_BlancoBlanco_X_Blancopopulation
FallgloFallglocultivar
Citrus paradisiCitrus paradisibreeding_research_material
Sweet OrangeSweet Orangebreeding_research_material
TempleTemplebreeding_research_material
Properties
Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Publication Date2010
Published Location|||
Language Abbreng
Publication Model[electronic resource].
URLhttp://dx.doi.org/10.1016/j.postharvbio.2010.07.013
Keywordstangerines, color, temporal variation, postharvest physiology, ripening, ethylene, ethylene production, signal transduction, gene expression, biochemical pathways, harvest date, genetic variation, hormone receptors, 1-aminocyclopropane-1-carboxylate synthase, aminocyclopropanecarboxylate oxidase, plant proteins, chlorophyllase, chlorophyll, chemical treatment, degreening, flavedo
Cross References
This publication is also available in the following databases:
DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:4431299