The complete genome sequence of 'Candidatus Liberibacter americanus', associated with Citrus huanglongbing

Publication Overview
TitleThe complete genome sequence of 'Candidatus Liberibacter americanus', associated with Citrus huanglongbing
AuthorsWulff NA, Zhang S, Setubal JC, Almeida NF, Martins EC, Harakava R, Kumar D, Rangel LT, Foissac X, Bové JM, Gabriel DW
TypeJournal Article
Journal NameMolecular plant-microbe interactions : MPMI
Volume27
Issue2
Year2014
Page(s)163-76
CitationWulff NA, Zhang S, Setubal JC, Almeida NF, Martins EC, Harakava R, Kumar D, Rangel LT, Foissac X, Bové JM, Gabriel DW. The complete genome sequence of 'Candidatus Liberibacter americanus', associated with Citrus huanglongbing. Molecular plant-microbe interactions : MPMI. 2014 Feb; 27(2):163-76.

Abstract

Liberibacter spp. form a Rhizobiaceae clade of phloem-limited pathogens of limited host range. Two obligately parasitic species have been sequenced: 'Candidatus Liberibacter asiaticus', which causes citrus huanglongbing (HLB) worldwide, and 'Ca. L. solanacearum', which causes potato "zebra chip" disease. A third (proposed) species, Liberibacter crescens, was isolated from mountain papaya, grown in axenic culture, and sequenced. In an effort to identify common host determinants, the complete genomic DNA sequence of a second HLB species, 'Ca. L. americanus' strain 'São Paulo' was determined. The circular genome of 1,195,201 bp had an average 31.12% GC content and 983 predicted protein encoding genes, 800 (81.4%) of which had a predicted function. There were 658 genes common to all sequenced Liberibacter spp. and only 8 genes common to 'Ca. L. americanus' and 'Ca. L. asiaticus' but not found in 'Ca. L. solanacearum'. Surprisingly, most of the lipopolysaccharide biosynthetic genes were missing from the 'Ca. L. americanus' genome, as well as OmpA and a key regulator of flagellin, all indicating a 'Ca. L. americanus' strategy of avoiding production of major pathogen-associated molecular patterns present in 'Ca. L. asiaticus' and 'Ca. L. solanacearum'. As with 'Ca. L. asiaticus', one of two 'Ca. L. americanus' prophages replicated as an excision plasmid and carried potential lysogenic conversion genes that appeared fragmentary or degenerated in 'Ca. L. solanacearum'.

Properties
Additional details for this publication include:
Property NameValue
pISSN0894-0282
Publication Date2014 Feb
Journal AbbreviationMol. Plant Microbe Interact.
DOI10.1094/MPMI-09-13-0292-R
Elocation10.1094/MPMI-09-13-0292-R
Publication ModelPrint
LanguageEnglish
Language Abbreng
Publication TypeJournal Article
ISSN0894-0282
Journal CountryUnited States
Publication TypeResearch Support, Non-U.S. Gov't
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PMID: PubMedPMID:24200077