EY660254 | Citrus Genome Database

Overview


Name	EY660254
Unique Name	EY660254
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	937

Libraries


Library Name	Type
Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY660254 vs. ExPASy Swiss-Prot
Match: CAMT2_MAIZE (Caffeoyl-CoA O-methyltransferase 2 OS=Zea mays GN=CCOAOMT2 PE=2 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 5.790e-25
Identity = 58/138 (42.03%), Postives = 84/138 (60.87%), Query Frame = 3

Query:  270 LYDYILRNV---REPEILRQLREETAGMRGSQMQVSPDQAQLLAMLVQILGAQRCIEVGVYTGYSSLAIALVLPESGCLVACERDARSLEVAKKYYERAGVSHKVKIKHGLAADSLKALILNGEA-SSYDFASADAEK 671
            LY YIL      REPE +++LRE TA    + M  S D+ Q L ML++++GA++ +E+GVYTGYS LA AL LPE G ++A + +  + E+      +AGV HK+  + G A   L  L+ + E   S+DFA  DA+K
Sbjct:   46 LYQYILDTSVYPREPESMKELREITAKHPWNLMTTSADEGQFLNMLIKLIGAKKTMEIGVYTGYSLLATALALPEDGTILAMDINRENYELGLPCINKAGVGHKIDFREGPALPVLDDLVADKEQHGSFDFAFVDADK 183

HSP 2 Score: 33.113 bits (74), Expect = 5.790e-25
Identity = 15/32 (46.88%), Postives = 20/32 (62.50%), Query Frame = 2

Query:  668 KKMYHEYFELLLQLIRDGGII*N*NVLWDGKI 763
            K  Y  Y E LL+L+R GG+I   N LW+G +
Sbjct:  183 KDNYLNYHERLLKLVRPGGLIGYDNTLWNGSV 214

[Close]

BLAST of EY660254 vs. ExPASy Swiss-Prot
Match: CAMT3_ARATH (Probable caffeoyl-CoA O-methyltransferase At4g26220 OS=Arabidopsis thaliana GN=At4g26220 PE=2 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 3.674e-24
Identity = 57/138 (41.30%), Postives = 80/138 (57.97%), Query Frame = 3

Query:  270 LYDYILRNV---REPEILRQLREETAGMRGSQMQVSPDQAQLLAMLVQILGAQRCIEVGVYTGYSSLAIALVLPESGCLVACERDARSLEVAKKYYERAGVSHKVKIKHGLAADSLKALILNG-EASSYDFASADAEK 671
            LY YIL      REPE+LR+LR  T     + M  +PD  QL+ ML+ ++ A++ IEVGV+TGYS L  AL LPE G ++A + +  S E+     ++AGV HK+  K   A  +L  L+ N      +DFA  DA+K
Sbjct:   15 LYKYILETSVYPREPEVLRELRNITHNHPQAGMATAPDAGQLMGMLLNLVNARKTIEVGVFTGYSLLLTALTLPEDGKVIAIDMNRDSYEIGLPVIKKAGVEHKIDFKESEALPALDELLNNKVNEGGFDFAFVDADK 152

HSP 2 Score: 33.113 bits (74), Expect = 3.674e-24
Identity = 16/34 (47.06%), Postives = 21/34 (61.76%), Query Frame = 2

Query:  668 KKMYHEYFELLLQLIRDGGII*N*NVLWDGKIAD 769
            K  Y  Y E L++LI+ GGII   N LW G +A+
Sbjct:  152 KLNYWNYHERLIRLIKVGGIIVYDNTLWGGSVAE 185

[Close]

BLAST of EY660254 vs. ExPASy Swiss-Prot
Match: CAMT1_MAIZE (Caffeoyl-CoA O-methyltransferase 1 OS=Zea mays GN=CCOAOMT1 PE=2 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 4.767e-24
Identity = 58/138 (42.03%), Postives = 84/138 (60.87%), Query Frame = 3

Query:  270 LYDYILRNV---REPEILRQLREETAGMRGSQMQVSPDQAQLLAMLVQILGAQRCIEVGVYTGYSSLAIALVLPESGCLVACERDARSLEVAKKYYERAGVSHKVKIKHGLAADSLKALILNGE-ASSYDFASADAEK 671
            LY YIL      REPE +++LRE TA    + M  S D+ Q L ML++++GA++ +E+GVYTGYS LA AL LPE G ++A + +  + E+     E+AGV+HK+  + G A   L  LI   +   S+DF   DA+K
Sbjct:   40 LYQYILDTSVYPREPESMKELREVTAKHPWNLMTTSADEGQFLNMLIKLIGAKKTMEIGVYTGYSLLATALALPEDGTILAMDINRENYELGLPCIEKAGVAHKIDFREGPALPVLDDLIAEEKNHGSFDFVFVDADK 177

HSP 2 Score: 30.8018 bits (68), Expect = 4.767e-24
Identity = 14/32 (43.75%), Postives = 20/32 (62.50%), Query Frame = 2

Query:  668 KKMYHEYFELLLQLIRDGGII*N*NVLWDGKI 763
            K  Y  Y E LL+L++ GG+I   N LW+G +
Sbjct:  177 KDNYLNYHERLLKLVKLGGLIGYDNTLWNGSV 208

[Close]

BLAST of EY660254 vs. ExPASy Swiss-Prot
Match: CAMT4_TOBAC (Caffeoyl-CoA O-methyltransferase 4 OS=Nicotiana tabacum GN=CCOAOMT4 PE=2 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 3.927e-23
Identity = 55/138 (39.86%), Postives = 86/138 (62.32%), Query Frame = 3

Query:  270 LYDYILRNV---REPEILRQLREETAGMRGSQMQVSPDQAQLLAMLVQILGAQRCIEVGVYTGYSSLAIALVLPESGCLVACERDARSLEVAKKYYERAGVSHKVKIKHGLAADSLKALILNGE-ASSYDFASADAEK 671
            LY YIL      REPE +++LRE TA    + M  S D+ Q L+ML++++ A+  +E+GV+TGYS LA A+ LP+ G ++A + +  + E+     E+AG++HK++ K G A   L  +I +G+   SYDF   DA+K
Sbjct:   24 LYQYILETSVYPREPEPMKELREITAKHPWNLMTTSADEGQFLSMLIKLINAKNTMEIGVFTGYSLLATAMALPDDGKILAMDINRENYEIGLPVIEKAGLAHKIEFKEGPALPVLDQMIEDGKYHGSYDFIFVDADK 161

HSP 2 Score: 28.4906 bits (62), Expect = 3.927e-23
Identity = 12/32 (37.50%), Postives = 19/32 (59.38%), Query Frame = 2

Query:  668 KKMYHEYFELLLQLIRDGGII*N*NVLWDGKI 763
            K  Y  Y + L+ L++ GG+I   N LW+G +
Sbjct:  161 KDNYLNYHKRLIDLVKIGGLIGYDNTLWNGSV 192

[Close]

BLAST of EY660254 vs. ExPASy Swiss-Prot
Match: CAMT1_TOBAC (Caffeoyl-CoA O-methyltransferase 1 OS=Nicotiana tabacum GN=CCOAOMT1 PE=1 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 3.929e-23
Identity = 55/138 (39.86%), Postives = 86/138 (62.32%), Query Frame = 3

Query:  270 LYDYILRNV---REPEILRQLREETAGMRGSQMQVSPDQAQLLAMLVQILGAQRCIEVGVYTGYSSLAIALVLPESGCLVACERDARSLEVAKKYYERAGVSHKVKIKHGLAADSLKALILNGE-ASSYDFASADAEK 671
            LY YIL      REPE +++LRE TA    + M  S D+ Q L+ML++++ A+  +E+GV+TGYS LA A+ LP+ G ++A + +  + E+     E+AG++HK++ K G A   L  +I +G+   SYDF   DA+K
Sbjct:   21 LYQYILETSVYPREPEPMKELREITAKHPWNLMTTSADEGQFLSMLIKLINAKNTMEIGVFTGYSLLATAMALPDDGKILAMDINRENYEIGLPVIEKAGLAHKIEFKEGPALPVLDQMIEDGKYHGSYDFIFVDADK 158

HSP 2 Score: 28.4906 bits (62), Expect = 3.929e-23
Identity = 12/32 (37.50%), Postives = 19/32 (59.38%), Query Frame = 2

Query:  668 KKMYHEYFELLLQLIRDGGII*N*NVLWDGKI 763
            K  Y  Y + L+ L++ GG+I   N LW+G +
Sbjct:  158 KDNYLNYHKRLIDLVKIGGLIGYDNTLWNGSV 189

[Close]

BLAST of EY660254 vs. ExPASy Swiss-Prot
Match: CAMT1_POPTR (Caffeoyl-CoA O-methyltransferase 1 OS=Populus trichocarpa GN=CCOAOMT1 PE=2 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 5.107e-23
Identity = 56/138 (40.58%), Postives = 84/138 (60.87%), Query Frame = 3

Query:  270 LYDYILRNV---REPEILRQLREETAGMRGSQMQVSPDQAQLLAMLVQILGAQRCIEVGVYTGYSSLAIALVLPESGCLVACERDARSLEVAKKYYERAGVSHKVKIKHGLAADSLKALILNGEA-SSYDFASADAEK 671
            LY YIL      REPE +++LRE TA    + M  S D+ Q L ML++++ A+  +E+GVYTGYS LA AL +PE G ++A + +  + E+     ++AGV+HK+  K G A   L  +I +G+   S+DF   DA+K
Sbjct:   29 LYQYILETSVYPREPECMKELREVTAKHPWNIMTTSADEGQFLNMLLKLVNAKNTMEIGVYTGYSLLATALAIPEDGKILAMDINRENYELGLPVIQKAGVAHKIDFKEGPALPVLDQMIEDGKCHGSFDFIFVDADK 166

HSP 2 Score: 29.261 bits (64), Expect = 5.107e-23
Identity = 12/32 (37.50%), Postives = 20/32 (62.50%), Query Frame = 2

Query:  668 KKMYHEYFELLLQLIRDGGII*N*NVLWDGKI 763
            K  Y  Y + L++L++ GG+I   N LW+G +
Sbjct:  166 KDNYINYHKRLIELVKVGGLIGYDNTLWNGSV 197

[Close]

BLAST of EY660254 vs. ExPASy Swiss-Prot
Match: CAMT_POPTM (Caffeoyl-CoA O-methyltransferase OS=Populus tremuloides PE=2 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 8.647e-23
Identity = 56/138 (40.58%), Postives = 84/138 (60.87%), Query Frame = 3

Query:  270 LYDYILRNV---REPEILRQLREETAGMRGSQMQVSPDQAQLLAMLVQILGAQRCIEVGVYTGYSSLAIALVLPESGCLVACERDARSLEVAKKYYERAGVSHKVKIKHGLAADSLKALILNGE-ASSYDFASADAEK 671
            LY YIL      REPE +++LRE TA    + M  S D+ Q L ML++++ A+  +E+GVYTGYS LA AL +PE G ++A + +  + E+     ++AGV+HK+  K G A   L  +I +G+   S+DF   DA+K
Sbjct:   29 LYQYILETSVYPREPECMKELREVTAKHPWNIMTTSADEGQFLNMLLKLVNAKNTMEIGVYTGYSLLATALAIPEDGKILAMDINRENYELGLPVIQKAGVAHKIDFKEGPALPVLDQMIEDGKYHGSFDFIFVDADK 166

HSP 2 Score: 29.261 bits (64), Expect = 8.647e-23
Identity = 12/32 (37.50%), Postives = 20/32 (62.50%), Query Frame = 2

Query:  668 KKMYHEYFELLLQLIRDGGII*N*NVLWDGKI 763
            K  Y  Y + L++L++ GG+I   N LW+G +
Sbjct:  166 KDNYINYHKRLIELVKVGGLIGYDNTLWNGSV 197

[Close]

BLAST of EY660254 vs. ExPASy Swiss-Prot
Match: CAMT_PETCR (Caffeoyl-CoA O-methyltransferase OS=Petroselinum crispum PE=1 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 1.126e-22
Identity = 54/138 (39.13%), Postives = 83/138 (60.14%), Query Frame = 3

Query:  270 LYDYILRNV---REPEILRQLREETAGMRGSQMQVSPDQAQLLAMLVQILGAQRCIEVGVYTGYSSLAIALVLPESGCLVACERDARSLEVAKKYYERAGVSHKVKIKHGLAADSLKALILNGE-ASSYDFASADAEK 671
            LY YIL      REPE +++LRE TA    + M  S D+ Q L ML++++ A+  +E+GVYTGYS LA AL LP+ G ++A + +  + E+     E+AGV HK+  + G A   L  ++ +G+   ++DF   DA+K
Sbjct:   23 LYQYILETSVYPREPEAMKELREVTAKHPWNLMTTSADEGQFLNMLLKLINAKNTMEIGVYTGYSLLATALALPDDGKILAMDINRENYEIGLPIIEKAGVGHKIDFREGPALPVLDHMLEDGKYHGTFDFVFVDADK 160

HSP 2 Score: 30.0314 bits (66), Expect = 1.126e-22
Identity = 13/33 (39.39%), Postives = 20/33 (60.61%), Query Frame = 2

Query:  668 KKMYHEYFELLLQLIRDGGII*N*NVLWDGKIA 766
            K  Y  Y + L+ L++ GG+I   N LW+G +A
Sbjct:  160 KDNYINYHKRLIDLVKIGGLIGYDNTLWNGSVA 192

[Close]

BLAST of EY660254 vs. ExPASy Swiss-Prot
Match: CAMT1_ARATH (Putative caffeoyl-CoA O-methyltransferase At1g67980 OS=Arabidopsis thaliana GN=At1g67980 PE=2 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 1.127e-22
Identity = 56/128 (43.75%), Postives = 81/128 (63.28%), Query Frame = 3

Query:  297 REPEILRQLREETAGMRG--SQMQVSPDQAQLLAMLVQILGAQRCIEVGVYTGYSSLAIALVLPESGCLVACERDARSLEVAKKYYERAGVSHKVKIKHGLAADSLKAL-ILNGEASSYDFASADAEK 671
            RE E+L++LR+ T    G  S+M+V  D+   L+MLV+I+ A+  IE+GV+TGYS L  AL LPE G + A + D  + EV  ++ ++AGV HK+   H   +D LKAL  L  +   +DFA ADA+K
Sbjct:   28 REHELLKELRKATVQKYGNLSEMEVPVDEGHFLSMLVKIMNAKNTIEIGVFTGYSLLTTALALPEDGRITAIDIDKEAYEVGLEFIKKAGVDHKINFIH---SDGLKALDQLVNDKCEFDFAFADADK 152

HSP 2 Score: 30.4166 bits (67), Expect = 1.127e-22
Identity = 15/34 (44.12%), Postives = 21/34 (61.76%), Query Frame = 2

Query:  668 KKMYHEYFELLLQLIRDGGII*N*NVLWDGKIAD 769
            K  Y  + E LL+L++ GGII   N LW G +A+
Sbjct:  152 KSSYVNFHERLLKLVKVGGIIAFDNTLWFGFVAE 185

[Close]

BLAST of EY660254 vs. ExPASy Swiss-Prot
Match: CAMT3_TOBAC (Caffeoyl-CoA O-methyltransferase 3 OS=Nicotiana tabacum GN=CCOAOMT3 PE=2 SV=2)

HSP 1 Score: 99.3673 bits (246), Expect = 1.465e-22
Identity = 55/138 (39.86%), Postives = 86/138 (62.32%), Query Frame = 3

Query:  270 LYDYILRNV---REPEILRQLREETAGMRGSQMQVSPDQAQLLAMLVQILGAQRCIEVGVYTGYSSLAIALVLPESGCLVACERDARSLEVAKKYYERAGVSHKVKIKHGLAADSLKALILNGE-ASSYDFASADAEK 671
            LY YIL      REPE +++LRE TA    + M  S D+ Q L+ML++++ A+  +E+GV+TGYS LA A+ LP+ G ++A + +  + E+     E+AG++HK++ K G A   L  +I +G+   SYDF   DA+K
Sbjct:   24 LYQYILETSVYPREPEPMKELREITAKHPWNIMTTSADEGQFLSMLLKLINAKNTMEIGVFTGYSLLATAMALPDDGKILAMDINRDNYEIGLPVIEKAGLAHKIEFKEGPALPVLDQMIEDGKYHGSYDFIFVDADK 161

HSP 2 Score: 28.4906 bits (62), Expect = 1.465e-22
Identity = 12/32 (37.50%), Postives = 19/32 (59.38%), Query Frame = 2

Query:  668 KKMYHEYFELLLQLIRDGGII*N*NVLWDGKI 763
            K  Y  Y + L+ L++ GG+I   N LW+G +
Sbjct:  161 KDNYLNYHKRLIDLVKVGGLIGYDNTLWNGSV 192

The following BLAST results are available for this feature:

BLAST of EY660254 vs. ExPASy Swiss-Prot

BLAST of EY660254 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 33


Match Name	E-value	Identity	Description
CAMT2_MAIZE	5.790e-25	42.03	Caffeoyl-CoA O-methyltransferase 2 OS=Zea mays GN=...	[more]
CAMT3_ARATH	3.674e-24	41.30	Probable caffeoyl-CoA O-methyltransferase At4g2622...	[more]
CAMT1_MAIZE	4.767e-24	42.03	Caffeoyl-CoA O-methyltransferase 1 OS=Zea mays GN=...	[more]
CAMT4_TOBAC	3.927e-23	39.86	Caffeoyl-CoA O-methyltransferase 4 OS=Nicotiana ta...	[more]
CAMT1_TOBAC	3.929e-23	39.86	Caffeoyl-CoA O-methyltransferase 1 OS=Nicotiana ta...	[more]
CAMT1_POPTR	5.107e-23	40.58	Caffeoyl-CoA O-methyltransferase 1 OS=Populus tric...	[more]
CAMT_POPTM	8.647e-23	40.58	Caffeoyl-CoA O-methyltransferase OS=Populus tremul...	[more]
CAMT_PETCR	1.126e-22	39.13	Caffeoyl-CoA O-methyltransferase OS=Petroselinum c...	[more]
CAMT1_ARATH	1.127e-22	43.75	Putative caffeoyl-CoA O-methyltransferase At1g6798...	[more]
CAMT3_TOBAC	1.465e-22	39.86	Caffeoyl-CoA O-methyltransferase 3 OS=Nicotiana ta...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C1-101-020-B12-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY660254 ID=EY660254; Name=EY660254; organism=Citrus sinensis; type=EST; length=937bp
TGCAAAAAAGGGGTTTCCGCAAACNGCNGTAACAAAATCAGGGTTTGCNN
GAGCAAATGGCGAGCAGCTTGGGGGAGAAACTGTGNGATCTTAAAAAAAG
GATAACTGAAAAAACGGTTGCCAAATTCCATTCCTTTCCCTTATCTGAAA
GATTGAGATTGAGACCCTGCAAGTTTAAGACTAAGAATCAGTTCACCACA
AACTGCTGTGTTTCCTCTGCCCATGACGAAAAGTACAGCAACAAGCAAGT
CATCAGTGTAACTCCTCCCCTTTACGACTACATCCTCCGAAATGTTCGTG
AGCCCGAGATTTTGCGTCAACTGCGGGAAGAGACTGCTGGTATGCGTGGT
AGCCAGATGCAGGTTTCGCCAGATCAAGCACAGCTACTTGCAATGCTTGT
ACAGATTCTCGGTGCACAACGGTGTATAGAAGTTGGTGTGTATACTGGAT
ACTCATCATTGGCTATTGCACTAGTGCTGCCGGAATCTGGTTGTCTTGTT
GCTTGTGAAAGAGATGCCAGGTCTCTTGAGGTTGCAAAAAAGTATTATGA
GCGAGCTGGTGTTTCGCATAAGGTAAAAATTAAGCATGGATTAGCAGCAG
ATTCCCTAAAAGCTCTGATTTTGAATGGCGAAGCTTCCAGCTATGATTTT
GCATCTGCTGATGCTGAAAAAAGATGTACCACGAGTACTTTGAATTGTTG
CTGCAACTGATCAGAGATGGGGGGATCATCTGAAATTGAAATGTCCTTTG
GGATGGAAAAATTGCTGACCAGATGGTGAATTGATGCTAAGACTTTTCCC
CATTAGGAATTTTTATTAAAATTTTATGGGAGGACGAACGGGTAAGCATT
AAATATGGTTCCTATTGGAAAATGGGATGACAATCTGTGCGGAAAAAATA
GACTTCATTTTTTTCTTGCACTTGTTTCCAAAAAAAA