EY660243 | Citrus Genome Database

Overview


Name	EY660243
Unique Name	EY660243
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	612

Libraries


Library Name	Type
Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig7838	contig	Csv1_Contig7838:1148..1753.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

BLAST of EY660243 vs. ExPASy Swiss-Prot
Match: TBA1_PEA (Tubulin alpha-1 chain OS=Pisum sativum GN=TUBA1 PE=3 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 1.210e-20
Identity = 40/40 (100.00%), Postives = 40/40 (100.00%), Query Frame = 2

Query:  128 HKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY 247
            HKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY
Sbjct:  393 HKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY 432

HSP 2 Score: 33.4982 bits (75), Expect = 1.210e-20
Identity = 15/19 (78.95%), Postives = 17/19 (89.47%), Query Frame = 3

Query:   69 KGVCMISNSTSVAEVFLRL 125
            + VCMISNSTSVAEVF R+
Sbjct:  373 RAVCMISNSTSVAEVFSRI 391

BLAST of EY660243 vs. ExPASy Swiss-Prot
Match: TBA_PRUDU (Tubulin alpha chain OS=Prunus dulcis GN=TUBA PE=2 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 1.210e-20
Identity = 40/40 (100.00%), Postives = 40/40 (100.00%), Query Frame = 2

Query:  128 HKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY 247
            HKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY
Sbjct:  393 HKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY 432

HSP 2 Score: 33.4982 bits (75), Expect = 1.210e-20
Identity = 15/19 (78.95%), Postives = 17/19 (89.47%), Query Frame = 3

Query:   69 KGVCMISNSTSVAEVFLRL 125
            + VCMISNSTSVAEVF R+
Sbjct:  373 RAVCMISNSTSVAEVFSRI 391

BLAST of EY660243 vs. ExPASy Swiss-Prot
Match: TBA6_ARATH (Tubulin alpha-6 chain OS=Arabidopsis thaliana GN=TUBA6 PE=2 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 1.210e-20
Identity = 40/40 (100.00%), Postives = 40/40 (100.00%), Query Frame = 2

Query:  128 HKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY 247
            HKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY
Sbjct:  393 HKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY 432

HSP 2 Score: 33.4982 bits (75), Expect = 1.210e-20
Identity = 15/19 (78.95%), Postives = 17/19 (89.47%), Query Frame = 3

Query:   69 KGVCMISNSTSVAEVFLRL 125
            + VCMISNSTSVAEVF R+
Sbjct:  373 RAVCMISNSTSVAEVFSRI 391

BLAST of EY660243 vs. ExPASy Swiss-Prot
Match: TBA2_ARATH (Tubulin alpha-2/alpha-4 chain OS=Arabidopsis thaliana GN=TUBA2 PE=2 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 1.210e-20
Identity = 40/40 (100.00%), Postives = 40/40 (100.00%), Query Frame = 2

Query:  128 HKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY 247
            HKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY
Sbjct:  393 HKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY 432

HSP 2 Score: 33.4982 bits (75), Expect = 1.210e-20
Identity = 15/19 (78.95%), Postives = 17/19 (89.47%), Query Frame = 3

Query:   69 KGVCMISNSTSVAEVFLRL 125
            + VCMISNSTSVAEVF R+
Sbjct:  373 RAVCMISNSTSVAEVFSRI 391

BLAST of EY660243 vs. ExPASy Swiss-Prot
Match: TBA1_GOSHI (Tubulin alpha-1 chain OS=Gossypium hirsutum PE=2 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 1.210e-20
Identity = 40/40 (100.00%), Postives = 40/40 (100.00%), Query Frame = 2

Query:  128 HKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY 247
            HKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY
Sbjct:  393 HKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY 432

HSP 2 Score: 33.4982 bits (75), Expect = 1.210e-20
Identity = 15/19 (78.95%), Postives = 17/19 (89.47%), Query Frame = 3

Query:   69 KGVCMISNSTSVAEVFLRL 125
            + VCMISNSTSVAEVF R+
Sbjct:  373 RAVCMISNSTSVAEVFSRI 391

BLAST of EY660243 vs. ExPASy Swiss-Prot
Match: TBA1_ANEPH (Tubulin alpha-1 chain OS=Anemia phyllitidis GN=TUBA1 PE=2 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 1.210e-20
Identity = 40/40 (100.00%), Postives = 40/40 (100.00%), Query Frame = 2

Query:  128 HKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY 247
            HKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY
Sbjct:  393 HKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY 432

HSP 2 Score: 33.4982 bits (75), Expect = 1.210e-20
Identity = 15/19 (78.95%), Postives = 17/19 (89.47%), Query Frame = 3

Query:   69 KGVCMISNSTSVAEVFLRL 125
            + VCMISNSTSVAEVF R+
Sbjct:  373 RAVCMISNSTSVAEVFSRI 391

BLAST of EY660243 vs. ExPASy Swiss-Prot
Match: TBA2_GOSHI (Tubulin alpha-2 chain OS=Gossypium hirsutum PE=2 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 1.574e-20
Identity = 39/40 (97.50%), Postives = 40/40 (100.00%), Query Frame = 2

Query:  128 HKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY 247
            HKFDLMYAKRAF+HWYVGEGMEEGEFSEAREDLAALEKDY
Sbjct:  393 HKFDLMYAKRAFIHWYVGEGMEEGEFSEAREDLAALEKDY 432

HSP 2 Score: 33.4982 bits (75), Expect = 1.574e-20
Identity = 15/19 (78.95%), Postives = 17/19 (89.47%), Query Frame = 3

Query:   69 KGVCMISNSTSVAEVFLRL 125
            + VCMISNSTSVAEVF R+
Sbjct:  373 RAVCMISNSTSVAEVFSRI 391

BLAST of EY660243 vs. ExPASy Swiss-Prot
Match: TBA1_STYLE (Tubulin alpha-1 chain OS=Stylonychia lemnae PE=3 SV=2)

HSP 1 Score: 86.2705 bits (212), Expect = 1.575e-20
Identity = 39/40 (97.50%), Postives = 39/40 (97.50%), Query Frame = 2

Query:  128 HKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY 247
            HKFDLMYAKRAFVHWYVGEGMEEGEFSE REDLAALEKDY
Sbjct:  388 HKFDLMYAKRAFVHWYVGEGMEEGEFSEVREDLAALEKDY 427

HSP 2 Score: 34.6538 bits (78), Expect = 1.575e-20
Identity = 19/39 (48.72%), Postives = 25/39 (64.10%), Query Frame = 3

Query:   12 CGTSPESLLDEASG-PCPV*KGVCMISNSTSVAEVFLRL 125
            CG + +      SG P  V + VCMISNST++AEVF R+
Sbjct:  348 CGINYQPPTVVPSGDPAKVMRAVCMISNSTAIAEVFSRI 386

BLAST of EY660243 vs. ExPASy Swiss-Prot
Match: TBA_TETTH (Tubulin alpha chain OS=Tetrahymena thermophila PE=1 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 2.048e-20
Identity = 40/40 (100.00%), Postives = 40/40 (100.00%), Query Frame = 2

Query:  128 HKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY 247
            HKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY
Sbjct:  393 HKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY 432

HSP 2 Score: 32.7278 bits (73), Expect = 2.048e-20
Identity = 14/19 (73.68%), Postives = 17/19 (89.47%), Query Frame = 3

Query:   69 KGVCMISNSTSVAEVFLRL 125
            + VCMISNST++AEVF RL
Sbjct:  373 RAVCMISNSTAIAEVFSRL 391

BLAST of EY660243 vs. ExPASy Swiss-Prot
Match: TBA_TETPY (Tubulin alpha chain OS=Tetrahymena pyriformis PE=3 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 2.048e-20
Identity = 40/40 (100.00%), Postives = 40/40 (100.00%), Query Frame = 2

Query:  128 HKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY 247
            HKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY
Sbjct:  393 HKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDY 432

HSP 2 Score: 32.7278 bits (73), Expect = 2.048e-20
Identity = 14/19 (73.68%), Postives = 17/19 (89.47%), Query Frame = 3

Query:   69 KGVCMISNSTSVAEVFLRL 125
            + VCMISNST++AEVF RL
Sbjct:  373 RAVCMISNSTAIAEVFSRL 391

The following BLAST results are available for this feature:

BLAST of EY660243 vs. ExPASy Swiss-Prot

BLAST of EY660243 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 140


Match Name	E-value	Identity	Description
TBA1_PEA	1.210e-20	100.00	Tubulin alpha-1 chain OS=Pisum sativum GN=TUBA1 PE...	[more]
TBA_PRUDU	1.210e-20	100.00	Tubulin alpha chain OS=Prunus dulcis GN=TUBA PE=2 ...	[more]
TBA6_ARATH	1.210e-20	100.00	Tubulin alpha-6 chain OS=Arabidopsis thaliana GN=T...	[more]
TBA2_ARATH	1.210e-20	100.00	Tubulin alpha-2/alpha-4 chain OS=Arabidopsis thali...	[more]
TBA1_GOSHI	1.210e-20	100.00	Tubulin alpha-1 chain OS=Gossypium hirsutum PE=2 S...	[more]
TBA1_ANEPH	1.210e-20	100.00	Tubulin alpha-1 chain OS=Anemia phyllitidis GN=TUB...	[more]
TBA2_GOSHI	1.574e-20	97.50	Tubulin alpha-2 chain OS=Gossypium hirsutum PE=2 S...	[more]
TBA1_STYLE	1.575e-20	97.50	Tubulin alpha-1 chain OS=Stylonychia lemnae PE=3 S...	[more]
TBA_TETTH	2.048e-20	100.00	Tubulin alpha chain OS=Tetrahymena thermophila PE=...	[more]
TBA_TETPY	2.048e-20	100.00	Tubulin alpha chain OS=Tetrahymena pyriformis PE=3...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C1-101-020-B01-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY660243 ID=EY660243; Name=EY660243; organism=Citrus sinensis; type=EST; length=612bp
CAGTGGTACCCTGCGGNACCTCTCCGGAATCCCTTCTCGACGAGGCTTCC
GGACCATGCCCTGTCTAAAAAGGTGTTTGCATGATCTCCAACTCCACCAG
TGTGGCAGAGGTGTTCTTGCGTCTGNACACAAGTTTGATCTTATGTATGC
AAAGCGTGCTTTCGTCCACTGGTATGTTGGCGAGGGTATGGAAGAAGGAG
AGTTCTCTGAGGCTCGTGAGGATCTTGCTGCCCTTGAGAAGGATTATGAG
GAGGTGGGTGCAGAGTCTGCCGAGGGCGAGGAAGATTATGGTGAGGAGGA
CTGAGGGATCTAAGTGTGCAATTATCTGTGCGTTTTAGTATGATGTCTTG
TGCTTTAGTGACGCTTCGTGCCTTTATTTTGTGTTGAAAACCTTATTCCT
CTCTTTGATTCTCGCTCAATTTCAAAGCTTGTTGAAATTTGTTATGCTTA
TCAAGTTTATGGGNTCTATATGTTCATGTAATCTTTGACTGTTTGATTTT
GATTGGATTATCTTATTTCAATTTACAACATAGCTTCNGGGGGTTTGTCT
AATATTGGTCGGATATTTACGTCAAATAAAGATAATTGATTTTCAAGAAA
AAAAAAAAAAAA