EY705315

Overview
NameEY705315
Unique NameEY705315
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length846
Libraries
Library NameType
Sweet orange fruit, development stadium (2 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig10176 contig Csv1_Contig10176:27..872. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
BLAST of EY705315 vs. ExPASy Swiss-Prot
Match: MIRA_RICDU (Miraculin OS=Richadella dulcifica PE=1 SV=3)

HSP 1 Score: 106.686 bits (265), Expect = 1.716e-22
Identity = 70/194 (36.08%), Postives = 102/194 (52.58%), Query Frame = 1
Query:   58 ATQPLLGISSNS-EPVLDIIGNKVKSTLEYKLIPVLDGAGGFSTHLGT--NGQ--CPLDVIQLSSPSERGDNLRLLPYDNYK--IVKVSTDVNLRFPVKVSVKPCN--EEPLWKVDSYDDP----LIVTGGSEGHPGAETLLNWFKIEKAGNFSYGGVYNIVHCPSVCLSCPRRCNKIGVSTKDGVRRLLLVGD 600
            A  PLL  + ++  PVLDI G K+++   Y ++PVL   GG  T   T  NG   CP  V+Q     +    L   P +N K  +V+VSTD+N+ F    +  PC      +W++D YD+      +  GG +G+PG ET+ +WFKIE+   F   G Y +V CP+VC SC  +C  +G+      RR L + D
Sbjct:   21 AANPLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVSATTPNGTFVCPPRVVQTRKEVDHDRPLAFFP-ENPKEDVVRVSTDLNINFS---AFMPCRWTSSTVWRLDKYDESTGQYFVTIGGVKGNPGPETISSWFKIEE---FCGSGFYKLVFCPTVCGSCKVKCGDVGIYIDQKGRRRLALSD 207          
BLAST of EY705315 vs. ExPASy Swiss-Prot
Match: ASP_THECC (21 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 4.993e-22
Identity = 64/179 (35.75%), Postives = 98/179 (54.75%), Query Frame = 1
Query:   91 SEPVLDIIGNKVKSTLEYKLIPVLDGAGGFSTHLG--TNGQCPLDVIQLSSPSERGDNLRLLPYDNYK-IVKVSTDVNLRFPVKVSVKPCNEEPLWKVDSYDDPL----IVTGGSEGHPGAETLLNWFKIEKAGNFSYGGVYNIVHCPSVCLSCPRRCNKIGVSTKDGVRRLLLVGDHE 606
            + PVLD  G+++++ ++Y ++  + GAGG    LG  T   CP  V+Q  S  + G  +     D+   +V+VSTDVN+ F V +  + C+   +W++D+YD+      + T G +G PG  TL +WFKIEKAG       Y    CPSVC SC   C+ IG  + D  +  L + D+E
Sbjct:   28 NSPVLDTDGDELQTGVQYYVLSSISGAGGGGLALGRATGQSCPEIVVQRRSDLDNGTPVIFSNADSKDDVVRVSTDVNIEF-VPIRDRLCSTSTVWRLDNYDNSAGKWWVTTDGVKGEPGPNTLCSWFKIEKAGVLG----YKFRFCPSVCDSCTTLCSDIGRHSDDDGQIRLALSDNE 201          
BLAST of EY705315 vs. ExPASy Swiss-Prot
Match: LSPI_CARPA (Latex serine proteinase inhibitor OS=Carica papaya PE=1 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 1.115e-13
Identity = 58/179 (32.40%), Postives = 91/179 (50.84%), Query Frame = 1
Query:   94 EPVLDIIGNKVKSTLEYKLIPVLDGAGGFSTHL---GTNGQCPLDVIQLSSPSERGDNLRLLPYDNYK--IVKVSTDVNLRFPVKVSVKPCNEEPLWKVDSYDDPL---IVTGGSEGHPGAETLLNWFKIEKAG-NFSYGGVYNIVHCPSVCLSCPRRCNKIGV-STKDGVRRLLLVGD 600
            +P++DI G  V   ++Y ++  + GAGG    +   G   +CPL V+Q   P + G+ +      N K  IV  S D+N++F + ++   CNE   WKVD +   +   +  GG +G+ G E+  + FKI+KAG  FS    Y    CPS   +    CN + +   K  +RRL+L  D
Sbjct:    4 KPIVDIDGKPVLYGVDYFVVSAIWGAGGGGLTVYGPGNKKKCPLSVVQ--DPFDNGEPIIFSAIKNVKDNIVFESVDLNVKFNITIN---CNETTAWKVDRFPGVIGWTVTLGGEKGYHGFESTHSMFKIKKAGLPFS----YKFHFCPSYPRTRLIPCNNVDIFFDKYRIRRLILTND 173          
BLAST of EY705315 vs. ExPASy Swiss-Prot
Match: SPI7_SOLTU (Serine protease inhibitor 7 OS=Solanum tuberosum PE=1 SV=2)

HSP 1 Score: 75.8702 bits (185), Expect = 3.244e-13
Identity = 58/194 (29.90%), Postives = 100/194 (51.55%), Query Frame = 1
Query:   73 LGISSNSEPVLDIIGNKVKSTLEYKLIPVLDGAGGFSTHLG----TNGQCPLDVIQLSSP-SERGDNLRLLPYDNY--KIVKVSTDVNLRFPVKVSVKPCNEEPLWKVDSYD----DPLIVTGGSEGHPGAETLLNWFKIEKAGNFSYGGVYNIVHCP-SVCLSCP-----RRCNKIGVSTKDGVRRLLLVGDH 603
            + + S++ PVLD+ G ++ S L Y++I    GA G   +LG    ++  C   + + +S     G  +R +   ++  + +  +  +N++F +  S K C    +WKV  YD      L+ TGG+ G   +    +WFKI K+  F     YN+++CP +  +SCP     + C K+GV  ++G RRL LV D+
Sbjct:   27 INLPSDATPVLDVAGKELDSRLSYRIISTFWGALGGDVYLGKSPNSDAPCANGIFRYNSDVGPSGTPVRFIGSSSHFGQGIFENELLNIQFAISTS-KLCVSYTIWKVGDYDASLGTMLLETGGTIGQADS----SWFKIVKSSQFG----YNLLYCPVTSTMSCPFSSDDQFCLKVGVVHQNGKRRLALVKDN 211          
BLAST of EY705315 vs. ExPASy Swiss-Prot
Match: SPI2_SOLTU (Serine protease inhibitor 2 OS=Solanum tuberosum PE=1 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 7.227e-13
Identity = 54/186 (29.03%), Postives = 95/186 (51.08%), Query Frame = 1
Query:   79 ISSNSEPVLDIIGNKVKSTLEYKLIPVLDGAGGFSTHLG----TNGQCPLDVIQLSSP-SERGDNLRLLPYDNY--KIVKVSTDVNLRFPVKVSVKPCNEEPLWKVDSYDDPL----IVTGGSEGHPGAETLLNWFKIEKAGNFSYGGVYNIVHCPSVCLSCPRRCNKIGVSTKDGVRRLLLVGDH 603
            + S++ PVLD+ G ++ S L Y++I    GA G   +LG    ++  C   + + +S     G  +R +   ++  + +  +  +N++F +  S K C    +WKV  YD  L    + TGG+ G   +    +WFKI K+    Y    N+++CP    S  + C+K+GV  ++G RRL LV ++
Sbjct:    1 LPSDATPVLDVTGKELDSRLSYRIISTFWGALGGDVYLGKSPNSDAPCANGIFRYNSDVGPSGTPVRFIGSSSHFGQGIFENELLNIQFAISTS-KLCVSYTIWKVGDYDASLGTMLLETGGTIGQADS----SWFKIVKSSQLGY----NLLYCPVTSSSDDQFCSKVGVVHQNGKRRLALVNEN 177          
BLAST of EY705315 vs. ExPASy Swiss-Prot
Match: SPI1_SOLTU (Serine protease inhibitor 1 OS=Solanum tuberosum PE=1 SV=2)

HSP 1 Score: 74.3294 bits (181), Expect = 9.438e-13
Identity = 59/198 (29.80%), Postives = 101/198 (51.01%), Query Frame = 1
Query:   61 TQPLLGISSNSEPVLDIIGNKVKSTLEYKLIPVLDGAGGFSTHLG----TNGQCPLDVIQLSSP-SERGDNLRLLPYDNY--KIVKVSTDVNLRFPVKVSVKPCNEEPLWKVDSYD----DPLIVTGGSEGHPGAETLLNWFKIEKAGNFSYGGVYNIVHCP-SVCLSCP-----RRCNKIGVSTKDGVRRLLLVGDH 603
            +Q  + + S++ PVLD+ G ++ S L Y++I    GA G   +LG    ++  C   V + +S     G  +R +   ++  + +  +  +N++F +  S K C    +WKV  YD      L+ TGG+ G   +    +WFKI K+        YN+++CP +  +SCP     + C K+GV  ++G RRL LV D+
Sbjct:   23 SQNPINLPSDATPVLDVTGKELDSRLSYRIISTFWGALGGDVYLGKSPNSDAPCANGVFRYNSDVGPSGTPVRFIGSSSHFGQGIFENELLNIQFAISTS-KLCVSYTIWKVGDYDASLGTMLLETGGTIGQADS----SWFKIVKSSQLG----YNLLYCPVTSTMSCPFSSDDQFCLKVGVVHQNGKRRLALVKDN 211          
BLAST of EY705315 vs. ExPASy Swiss-Prot
Match: SPI6_SOLTU (Probable serine protease inhibitor 6 OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 6.118e-12
Identity = 57/198 (28.79%), Postives = 100/198 (50.51%), Query Frame = 1
Query:   61 TQPLLGISSNSEPVLDIIGNKVKSTLEYKLIPVLDGAGGFSTHLG----TNGQCPLDVIQLSSP-SERGDNLRLLPYDNY--KIVKVSTDVNLRFPVKVSVKPCNEEPLWKVDSYD----DPLIVTGGSEGHPGAETLLNWFKIEKAGNFSYGGVYNIVHCP-SVCLSCP-----RRCNKIGVSTKDGVRRLLLVGDH 603
            +Q  + + S++ PVLD+ G ++   L Y +I    GA G   +LG    ++  C   + + +S     G  +R +   ++  + +  +  +N++F +  S K C    +WKV  YD      L+ TGG+ G   +    +WFKI ++  F     YN+++CP +  +SCP     + C K+GV  ++G RRL LV D+
Sbjct:   23 SQNPINLPSDATPVLDVTGKELDPRLSYHIISTFWGALGGDVYLGKSPNSDAPCANGIFRYNSDVGPSGTPVRFIGSSSHFGQGIFENELLNIQFAISTS-KLCVSYTIWKVGDYDASLGTMLLETGGTIGQADS----SWFKIVQSSQFG----YNLLYCPVTSTMSCPFSSDDQFCLKVGVVHQNGKRRLALVKDN 211          
BLAST of EY705315 vs. ExPASy Swiss-Prot
Match: SPI5_SOLTU (Serine protease inhibitor 5 OS=Solanum tuberosum PE=1 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 3.036e-11
Identity = 56/192 (29.17%), Postives = 96/192 (50.00%), Query Frame = 1
Query:   61 TQPLLGISSNSEPVLDIIGNKVKSTLEYKLIPVLDGAGGFSTHLG----TNGQCPLDVIQLSSP-SERGDNLRLLPYDNY--KIVKVSTDVNLRFPVKVSVKPCNEEPLWKVDSYD----DPLIVTGGSEGHPGAETLLNWFKIEKAGNFSYGGVYNIVHCPSVCLSCPRRCNKIGVSTKDGVRRLLLVGDH 603
            +Q  + + S++ PVLD+ G ++   L Y++I +  GA G   +LG    ++  C   V + +S     G  +R +   ++    +     +N++F +  +VK C    +WKV  YD      L+ TGG+ G   +    +WFKI K+        YN+++CP    S  + C K+GV  ++G RRL LV D+
Sbjct:   23 SQNPINLPSDATPVLDVTGKELDPRLSYRIISIGRGALGGDVYLGKSPNSDAPCANGVFRFNSDVGPSGTPVRFIGSSSHFGPHIFEGELLNIQFDIS-TVKLCVSYTIWKVGDYDASLGTMLLETGGTIGQADS----SWFKIVKSSQLG----YNLLYCP--FSSDDQFCLKVGVVHQNGKRRLALVKDN 203          
BLAST of EY705315 vs. ExPASy Swiss-Prot
Match: DRTI_DELRE (Kunitz-type serine protease inhibitor DrTI OS=Delonix regia PE=1 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 3.965e-11
Identity = 56/173 (32.37%), Postives = 83/173 (47.98%), Query Frame = 1
Query:   85 SNSEPVLDIIGNKVKSTLEYKLIPVLDGAGGFSTHLG-TNGQ-CPLDVIQLSSPSERGDNLRLL-PYDNYKIVKVSTDVNLRFPVKVSVKPCNEEPLWK-VDSYDDPLIVTGGSEGHPGAETLLNWFKIEKAGNFSYGGVYNIVHCPSVCLSCPRRCNKIGVSTKDGVRRLLL 591
            S++E V DI G  V    EY ++  + GAGG     G T G  CP+ +IQ  S  + G  +R   P ++   +   T++ + F   V    C E   W  V    +  +  GGSE HP  E +  +FKIEK G+ +    Y +V CP    S    C+ IG++  +G R L+L
Sbjct:    1 SDAEKVYDIEGYPVFLGSEYYIVSAIIGAGGGGVRPGRTRGSMCPMSIIQEQSDLQMGLPVRFSSPEESQGKIYTDTELEIEF---VEKPDCAESSKWVIVKDSGEARVAIGGSEDHPQGELVRGFFKIEKLGSLA----YKLVFCPK---SSSGSCSDIGIN-YEGRRSLVL 162          
BLAST of EY705315 vs. ExPASy Swiss-Prot
Match: API9_SOLTU (Aspartic protease inhibitor 9 OS=Solanum tuberosum PE=1 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 3.965e-11
Identity = 55/184 (29.89%), Postives = 87/184 (47.28%), Query Frame = 1
Query:   94 EPVLDIIGNKVKSTLEYKLIPVLDGAGGFSTHLG----TNGQCPLDVIQLSSP-SERGDNLRLLPYDNYKIVKVSTDVNLRFPVKVSVKPCNEEPLWKVDS----YDDPLIVTGGSEGHPGAETLLNWFKIEKAGNFSYGGVYNIVHCP---SVCLSCP--RRCNKIGVSTKDGVRRLLLVGDH 603
            +PVLD  G ++     Y++I +  GA G   +LG    ++  CP  V + +S     G  +R +P           ++    P   +VK C    +WKV +    +   L+ TGG+ G        ++FKI K  NF     YN++ CP    +CL CP  + C K+GV  ++G RRL LV ++
Sbjct:    6 KPVLDTNGKELNPNSSYRIISIGAGALGGDVYLGKSPNSDAPCPDGVFRYNSDVGPSGTPVRFIPLSGGIFEDQLLNIQFNIP---TVKLCVSYTIWKVGNLNAYFRTMLLETGGTIGQADN----SYFKIVKLSNFG----YNLLSCPFTSIICLRCPEDQFCAKVGVVIQNGKRRLALVNEN 178          
The following BLAST results are available for this feature:
BLAST of EY705315 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 11
Match NameE-valueIdentityDescription
MIRA_RICDU1.716e-2236.08Miraculin OS=Richadella dulcifica PE=1 SV=3[more]
ASP_THECC4.993e-2235.7521 kDa seed protein OS=Theobroma cacao GN=ASP PE=2... [more]
LSPI_CARPA1.115e-1332.40Latex serine proteinase inhibitor OS=Carica papaya... [more]
SPI7_SOLTU3.244e-1329.90Serine protease inhibitor 7 OS=Solanum tuberosum P... [more]
SPI2_SOLTU7.227e-1329.03Serine protease inhibitor 2 OS=Solanum tuberosum P... [more]
SPI1_SOLTU9.438e-1329.80Serine protease inhibitor 1 OS=Solanum tuberosum P... [more]
SPI6_SOLTU6.118e-1228.79Probable serine protease inhibitor 6 OS=Solanum tu... [more]
SPI5_SOLTU3.036e-1129.17Serine protease inhibitor 5 OS=Solanum tuberosum P... [more]
DRTI_DELRE3.965e-1132.37Kunitz-type serine protease inhibitor DrTI OS=Delo... [more]
API9_SOLTU3.965e-1129.89Aspartic protease inhibitor 9 OS=Solanum tuberosum... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-701-042-B12-CT.F Sweet orange fruit, development stadium (2 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY705315 ID=EY705315; Name=EY705315; organism=Citrus sinensis; type=EST; length=846bp
CCACGCGTCCGGAAGACTTCATTGACAACAACACTCTCCTTCCTTATCCT
TACCTTGGCCACGCAGCCTCTATTGGGCATCTCATCAAACTCTGAACCTG
TCTTAGATATCATTGGCAACAAGGTCAAATCCACCCTCGAGTATAAACTT
ATCCCAGTCCTAGATGGCGCCGGTGGATTCAGCACACACCTAGGCACAAA
TGGACAGTGTCCCTTGGACGTTATCCAATTAAGTTCTCCTAGCGAAAGGG
GAGATAATTTAAGATTACTTCCGTACGACAATTACAAAATTGTCAAGGTA
TCCACTGATGTCAACCTGAGATTCCCCGTAAAAGTTTCTGTTAAGCCCTG
TAATGAAGAACCATTGTGGAAAGTTGATAGCTATGACGATCCATTAATTG
TAACCGGCGGAAGTGAGGGCCACCCAGGAGCTGAAACGTTGCTAAATTGG
TTTAAGATTGAGAAAGCTGGAAATTTTTCATATGGTGGTGTATACAATAT
TGTCCATTGTCCGAGTGTCTGTCTATCTTGTCCTAGACGCTGCAACAAAA
TTGGCGTTTCAACTAAGGATGGCGTTCGACGTCTGCTTCTAGTTGGTGAT
CATGAACCGGCTTTGAATGGGTCTGTTTTTCCATCAGAGGAAAGCTNCAG
GCTACTTNCAGCTGCTAACGCAATATCAAGGGTTTTTGAAGCTGCTGAAG
CATATCAATGACCGCGTAATGAATGAACTTAGCTGTATCATTCTAAATAA
TAGACCAGGCTAGTAACCTTTTATCTCTATTACAGAAATAAAATAACAAC
GCCTGTGATCCTTTTGCTGTTGGTAAAAAAAAAAAAAAAAAGGCCG
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