EY675590

Overview
NameEY675590
Unique NameEY675590
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length970
Libraries
Library NameType
Sweet orange leaf, infected with CiLVcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY675590 vs. ExPASy Swiss-Prot
Match: SAC1_DROME (Phosphatidylinositide phosphatase SAC1 OS=Drosophila melanogaster GN=Sac1 PE=1 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 9.495e-15
Identity = 40/109 (36.70%), Postives = 65/109 (59.63%), Query Frame = 3 
Query:   12 RMWRRGPNLEGDTANFIETDQLLETEGFKSSLLQVRGSIPLLWEQIVDLSYKPQLKIINHDQTPKVVERHFRVLLQRYGEIVAVDLTDKHGDEGQLSAAYAAEMQKLPN 338
            R++ RG + +G  ANF+ET+Q++E  G  +  +Q RGS+P  W Q+ +L YKP+  ++           HF+  ++ YG  VAV+L D  G EG+L A YA  ++++ N
Sbjct:  221 RLFCRGSDEQGHVANFVETEQIVEFNGQLTGFVQTRGSMPFHWHQLPNLRYKPRPVLVPGKDHLAACGLHFKEQIRLYGNNVAVNLVDHKGAEGELEATYARLVREMGN 329
[Close]
BLAST of EY675590 vs. ExPASy Swiss-Prot
Match: SAC1_DICDI (Phosphatidylinositide phosphatase SAC1 OS=Dictyostelium discoideum GN=sac1 PE=3 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 1.240e-14
Identity = 43/108 (39.81%), Postives = 66/108 (61.11%), Query Frame = 3 
Query:   24 RGPNLEGDTANFIETDQLLET-EGFKSSLLQVRGSIPLLWEQIVDLSYKPQLKIINHD-QTPKVVERHFRVLLQRYGEIVAVDLTDKHGDEGQLSAAYAAEMQKLPNI 341
            RG + +G+ AN +ET+Q++E  E   +S +QVRGSIPLLW Q   L YKP +K    + +  + +E+HF+ L Q YG    V+L D+ G E +L  AY   ++ L ++
Sbjct:  221 RGIDKKGNVANNVETEQIIEIKENTFTSFVQVRGSIPLLWSQFPTLKYKPSVKFYGDEKENSQALEQHFKQLHQLYGSTTVVNLIDRKGAELKLGEAYEERVKSLKDV 328
[Close]
BLAST of EY675590 vs. ExPASy Swiss-Prot
Match: SAC1A_DANRE (Phosphatidylinositide phosphatase SAC1-A OS=Danio rerio GN=sacm1la PE=2 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.700e-13
Identity = 41/109 (37.61%), Postives = 62/109 (56.88%), Query Frame = 3 
Query:   12 RMWRRGPNLEGDTANFIETDQLLETEGFKSSLLQVRGSIPLLWEQIVDLSYKPQLKIINHDQTPKVVERHFRVLLQRYGEIVAVDLTDKHGDEGQLSAAYAAEMQKLPN 338
            R + RG + EG  ANF+ET+Q+++  G K+S +Q RGSIP  W Q  +L YKP+ +I          +RHF   +  YG+ V ++L ++ G E  L  A+A  +  L N
Sbjct:  221 RYYVRGIDSEGHAANFVETEQIIQYNGAKASFIQTRGSIPFYWSQRPNLKYKPKPQISKSINHLDGFQRHFDSQIIIYGKQVILNLVNQKGSEKPLEQAFAKMVGSLGN 329

HSP 2 Score: 21.1718 bits (43), Expect = 1.700e-13
Identity = 6/10 (60.00%), Postives = 8/10 (80.00%), Query Frame = 1 
Query:  340 YVSFDFHHVC 369
            Y++FDFH  C
Sbjct:  334 YIAFDFHKEC 343
[Close]
BLAST of EY675590 vs. ExPASy Swiss-Prot
Match: SAC1_BOVIN (Phosphatidylinositide phosphatase SAC1 OS=Bos taurus GN=SACM1L PE=2 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 3.725e-13
Identity = 43/112 (38.39%), Postives = 63/112 (56.25%), Query Frame = 3 
Query:   12 RMWRRGPNLEGDTANFIETDQLLETEGFKSSLLQVRGSIPLLWEQIVDLSYKPQ---LKIINHDQTPKVVERHFRVLLQRYGEIVAVDLTDKHGDEGQLSAAYAAEMQKLPN 338
            R + RG + EG  ANF+ET+Q++   G ++S +Q RGSIPL W Q  +L YKP     K+ NH       +RHF   +  YG+ V ++L ++ G E  L  A+A  +  L N
Sbjct:  222 RYYVRGIDSEGHAANFVETEQIVHYNGSRASFVQTRGSIPLYWSQRPNLKYKPLPLINKVANHMDG---FQRHFDSQIIIYGKQVIINLVNQKGSEKPLEQAFATMVSSLGN 330

HSP 2 Score: 21.1718 bits (43), Expect = 3.725e-13
Identity = 6/10 (60.00%), Postives = 8/10 (80.00%), Query Frame = 1 
Query:  340 YVSFDFHHVC 369
            Y++FDFH  C
Sbjct:  335 YIAFDFHKEC 344
[Close]
BLAST of EY675590 vs. ExPASy Swiss-Prot
Match: SAC1B_DANRE (Phosphatidylinositide phosphatase SAC1-B OS=Danio rerio GN=sacm1lb PE=2 SV=2)

HSP 1 Score: 72.7886 bits (177), Expect = 1.787e-12
Identity = 38/109 (34.86%), Postives = 60/109 (55.05%), Query Frame = 3 
Query:   12 RMWRRGPNLEGDTANFIETDQLLETEGFKSSLLQVRGSIPLLWEQIVDLSYKPQLKIINHDQTPKVVERHFRVLLQRYGEIVAVDLTDKHGDEGQLSAAYAAEMQKLPN 338
            R + RG + EG  ANF+ET+Q+++    ++S +Q RGSIP  W Q  +L YKP+  I         + RHF   +  YG+ V ++L ++ G E  L  A+A  +  + N
Sbjct:  221 RYYVRGIDSEGHAANFVETEQIVQFNNARASFVQTRGSIPFFWSQRPNLKYKPKPLISKDTNHMDGLRRHFESQVLIYGKQVILNLVNQKGSELPLEQAFAKMVSSMEN 329

HSP 2 Score: 21.1718 bits (43), Expect = 1.787e-12
Identity = 6/10 (60.00%), Postives = 8/10 (80.00%), Query Frame = 1 
Query:  340 YVSFDFHHVC 369
            Y++FDFH  C
Sbjct:  334 YIAFDFHKEC 343
[Close]
BLAST of EY675590 vs. ExPASy Swiss-Prot
Match: SAC1_PONAB (Phosphatidylinositide phosphatase SAC1 OS=Pongo abelii GN=SACM1L PE=2 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 2.320e-12
Identity = 39/107 (36.45%), Postives = 59/107 (55.14%), Query Frame = 3 
Query:   12 RMWRRGPNLEGDTANFIETDQLLETEGFKSSLLQVRGSIPLLWEQIVDLSYKPQLKIINHDQTPKVVERHFRVLLQRYGEIVAVDLTDKHGDEGQLSAAYAAEMQKL 332
            R + RG + EG  ANF+ET+Q++   G K+S +Q RGSIP+ W Q  +L YKP  +I          +RHF   +  YG+ V ++L ++ G E  L   +A  +  L
Sbjct:  222 RYYVRGIDSEGHAANFVETEQIVHYNGSKASFVQTRGSIPVFWSQRPNLKYKPLPQISKVANHMDGFQRHFDSQVIIYGKQVIINLINQKGSEKPLEQTFATMVSSL 328

HSP 2 Score: 21.1718 bits (43), Expect = 2.320e-12
Identity = 6/10 (60.00%), Postives = 8/10 (80.00%), Query Frame = 1 
Query:  340 YVSFDFHHVC 369
            Y++FDFH  C
Sbjct:  335 YIAFDFHKEC 344
[Close]
BLAST of EY675590 vs. ExPASy Swiss-Prot
Match: SAC1_HUMAN (Phosphatidylinositide phosphatase SAC1 OS=Homo sapiens GN=SACM1L PE=1 SV=2)

HSP 1 Score: 72.4034 bits (176), Expect = 2.320e-12
Identity = 39/107 (36.45%), Postives = 59/107 (55.14%), Query Frame = 3 
Query:   12 RMWRRGPNLEGDTANFIETDQLLETEGFKSSLLQVRGSIPLLWEQIVDLSYKPQLKIINHDQTPKVVERHFRVLLQRYGEIVAVDLTDKHGDEGQLSAAYAAEMQKL 332
            R + RG + EG  ANF+ET+Q++   G K+S +Q RGSIP+ W Q  +L YKP  +I          +RHF   +  YG+ V ++L ++ G E  L   +A  +  L
Sbjct:  222 RYYVRGIDSEGHAANFVETEQIVHYNGSKASFVQTRGSIPVFWSQRPNLKYKPLPQISKVANHMDGFQRHFDSQVIIYGKQVIINLINQKGSEKPLEQTFATMVSSL 328

HSP 2 Score: 21.1718 bits (43), Expect = 2.320e-12
Identity = 6/10 (60.00%), Postives = 8/10 (80.00%), Query Frame = 1 
Query:  340 YVSFDFHHVC 369
            Y++FDFH  C
Sbjct:  335 YIAFDFHKEC 344
[Close]
BLAST of EY675590 vs. ExPASy Swiss-Prot
Match: SAC1_XENLA (Phosphatidylinositide phosphatase SAC1 OS=Xenopus laevis GN=sacm1l PE=2 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 3.012e-12
Identity = 39/109 (35.78%), Postives = 60/109 (55.05%), Query Frame = 3 
Query:   12 RMWRRGPNLEGDTANFIETDQLLETEGFKSSLLQVRGSIPLLWEQIVDLSYKPQLKIINHDQTPKVVERHFRVLLQRYGEIVAVDLTDKHGDEGQLSAAYAAEMQKLPN 338
            R + RG + EG  ANF+ET+Q++   G K+S +Q RGSIP  W Q  +L YKP+ +I          +RHF   +  YG+ V ++L ++ G E  L   ++  +  L N
Sbjct:  221 RYYVRGIDSEGHAANFVETEQIVHYNGNKASFVQTRGSIPFYWSQRPNLKYKPKPQISKAVNHMDGFQRHFDSQVISYGKQVVLNLVNQKGSEKPLEQEFSQMVSGLGN 329

HSP 2 Score: 21.1718 bits (43), Expect = 3.012e-12
Identity = 6/10 (60.00%), Postives = 8/10 (80.00%), Query Frame = 1 
Query:  340 YVSFDFHHVC 369
            Y++FDFH  C
Sbjct:  334 YIAFDFHKEC 343
[Close]
BLAST of EY675590 vs. ExPASy Swiss-Prot
Match: SAC1_MOUSE (Phosphatidylinositide phosphatase SAC1 OS=Mus musculus GN=Sacm1l PE=2 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 6.593e-12
Identity = 38/107 (35.51%), Postives = 58/107 (54.21%), Query Frame = 3 
Query:   12 RMWRRGPNLEGDTANFIETDQLLETEGFKSSLLQVRGSIPLLWEQIVDLSYKPQLKIINHDQTPKVVERHFRVLLQRYGEIVAVDLTDKHGDEGQLSAAYAAEMQKL 332
            R + RG + EG  ANF+ET+Q++   G ++S +Q RGSIP+ W Q  +L YKP  +I          +RHF   +  YG+ V ++L +  G E  L   +A  +  L
Sbjct:  222 RYYVRGIDSEGHAANFVETEQIVHYSGNRASFVQTRGSIPIFWSQRPNLKYKPHPQISKVANHMDGFQRHFDSQVIIYGKQVIINLVNHKGSEKPLEQTFANMVSSL 328

HSP 2 Score: 21.1718 bits (43), Expect = 6.593e-12
Identity = 6/10 (60.00%), Postives = 8/10 (80.00%), Query Frame = 1 
Query:  340 YVSFDFHHVC 369
            Y++FDFH  C
Sbjct:  335 YIAFDFHKEC 344
[Close]
BLAST of EY675590 vs. ExPASy Swiss-Prot
Match: SAC1_RAT (Phosphatidylinositide phosphatase SAC1 OS=Rattus norvegicus GN=Sacm1l PE=1 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 8.559e-12
Identity = 38/107 (35.51%), Postives = 58/107 (54.21%), Query Frame = 3 
Query:   12 RMWRRGPNLEGDTANFIETDQLLETEGFKSSLLQVRGSIPLLWEQIVDLSYKPQLKIINHDQTPKVVERHFRVLLQRYGEIVAVDLTDKHGDEGQLSAAYAAEMQKL 332
            R + RG + EG  ANF+ET+Q++   G ++S +Q RGSIP+ W Q  +L YKP  +I          +RHF   +  YG+ V ++L +  G E  L   +A  +  L
Sbjct:  222 RYYVRGIDSEGHAANFVETEQIVHYSGNRASFVQTRGSIPVFWSQRPNLKYKPDPQINKVANHMDGFQRHFDSQVIIYGKQVIINLVNHKGSEKPLEQTFAKMVSSL 328

HSP 2 Score: 21.1718 bits (43), Expect = 8.559e-12
Identity = 6/10 (60.00%), Postives = 8/10 (80.00%), Query Frame = 1 
Query:  340 YVSFDFHHVC 369
            Y++FDFH  C
Sbjct:  335 YIAFDFHKEC 344
The following BLAST results are available for this feature:
BLAST of EY675590 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 11
Match NameE-valueIdentityDescription
SAC1_DROME9.495e-1536.70Phosphatidylinositide phosphatase SAC1 OS=Drosophi... [more]
SAC1_DICDI1.240e-1439.81Phosphatidylinositide phosphatase SAC1 OS=Dictyost... [more]
SAC1A_DANRE1.700e-1337.61Phosphatidylinositide phosphatase SAC1-A OS=Danio ... [more]
SAC1_BOVIN3.725e-1338.39Phosphatidylinositide phosphatase SAC1 OS=Bos taur... [more]
SAC1B_DANRE1.787e-1234.86Phosphatidylinositide phosphatase SAC1-B OS=Danio ... [more]
SAC1_PONAB2.320e-1236.45Phosphatidylinositide phosphatase SAC1 OS=Pongo ab... [more]
SAC1_HUMAN2.320e-1236.45Phosphatidylinositide phosphatase SAC1 OS=Homo sap... [more]
SAC1_XENLA3.012e-1235.78Phosphatidylinositide phosphatase SAC1 OS=Xenopus ... [more]
SAC1_MOUSE6.593e-1235.51Phosphatidylinositide phosphatase SAC1 OS=Mus musc... [more]
SAC1_RAT8.559e-1235.51Phosphatidylinositide phosphatase SAC1 OS=Rattus n... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-401-012-F02-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY675590 ID=EY675590; Name=EY675590; organism=Citrus sinensis; type=EST; length=970bp
CCACGCGTCCGCGAATGTGGAGAAGAGGACCTAATCTTGAAGGTGACACC
GCTAATTTTATAGAAACTGACCAGTTACTAGAGACGGAAGGTTTTAAATC
CTCACTATTGCAGGTTCGGGGCTCAATACCACTCCTCTGGGAGCAGATTG
TTGATTTGAGTTATAAGCCACAGCTTAAAATTATTAATCATGACCAAACA
CCAAAAGTTGTGGAGCGCCATTTCCGTGTTCTATTACAAAGATATGGAGA
AATTGTAGCAGTAGACCTTACCGATAAGCATGGTGATGAAGGTCAATTGA
GTGCAGCATATGCGGCTGAAATGCAGAAGCTTCCAAATATATGTCTCATT
TGACTTTCATCATGTTTGTGGCACTTCAAACTTTTGATAAGCTGCAAGTT
CTGTATGATCAGATTCAACAGGAGTTTTGATAACCAAGGCTGAACAGATA
TCTCCTCATAGACCCAGAAAGAAATATATTTAGAAGGGAGCAGAAAGGAG
TTATCACAATCCAACTGTATTGATTGTCTTTGATCAACAAAATGTCACCC
CAAAAGTAACCTGGGCTCAAAAAGTTTCCTAAATTTTACAGTTGCAAAAG
AATTGGGAATATTGTCTTCCCACATAGTGCATTTCCATTGTTCCAGAGGG
AAAAATGGAAAATTTCAGAATGTTTGTGGGGTTAAACAAGGGCACCAAAA
TAACTCACCCAAATTGCTAGGAACAATTGCGCCCGAAAGGGAAAACCATT
GTTCGAAAAGGGAAAGAGAAACAACAATGGGAGAGGGAGTCTGAAGTGGG
GGAAGGGGATATCTATTACAGAAACAAAATTAAAGGATGTGTTCTCAAGA
ACGGAGTATTAAAAGAAGGAGCCAATGACGAGAAGATAAATTAGAAGATA
TAAATTAATATCTTGTATAGAAATAAACAATATTAGGCTCTCTCGTAATT
TTTTGTGATTTTGATATTGG
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