EY675161

Overview
NameEY675161
Unique NameEY675161
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length983
Libraries
Library NameType
Sweet orange leaf, infected with CiLVcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
EY675161_ssr471 microsatellite EY675161_ssr471:471..480. BLAST: Citrus ESTs to Prunus persica proteins V1
Homology
[Close]
BLAST of EY675161 vs. ExPASy Swiss-Prot
Match: PEL1_ARATH (Probable pectate lyase 1 OS=Arabidopsis thaliana GN=At1g04680 PE=2 SV=2)

HSP 1 Score: 62.3882 bits (150), Expect = 1.073e-12
Identity = 27/33 (81.82%), Postives = 31/33 (93.94%), Query Frame = 2 
Query:   80 VNPKPGTLHHAVIQDKPLWIVFPRDMVIQLRKE 178
            VNP+PGTL HAVIQD+PLWIVF RDMVIQL++E
Sbjct:  131 VNPRPGTLRHAVIQDRPLWIVFKRDMVIQLKQE 163

HSP 2 Score: 32.3426 bits (72), Expect = 1.073e-12
Identity = 13/15 (86.67%), Postives = 14/15 (93.33%), Query Frame = 1 
Query:   22 LGPRDGRFYVVTDPR 66
            +G RDGRFYVVTDPR
Sbjct:  112 IGGRDGRFYVVTDPR 126
[Close]
BLAST of EY675161 vs. ExPASy Swiss-Prot
Match: PEL15_ARATH (Probable pectate lyase 15 OS=Arabidopsis thaliana GN=At4g13710 PE=2 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 1.123e-11
Identity = 26/36 (72.22%), Postives = 33/36 (91.67%), Query Frame = 2 
Query:   71 QEKVNPKPGTLHHAVIQDKPLWIVFPRDMVIQLRKE 178
            ++ VNPKPGTL HAVIQ++PLWIVF RDMVI+L++E
Sbjct:  169 EDVVNPKPGTLRHAVIQEEPLWIVFKRDMVIELKQE 204

HSP 2 Score: 30.0314 bits (66), Expect = 1.123e-11
Identity = 11/14 (78.57%), Postives = 13/14 (92.86%), Query Frame = 1 
Query:   22 LGPRDGRFYVVTDP 63
            +G RDGRFY+VTDP
Sbjct:  153 IGGRDGRFYIVTDP 166
[Close]
BLAST of EY675161 vs. ExPASy Swiss-Prot
Match: PEL8_ARATH (Probable pectate lyase 8 OS=Arabidopsis thaliana GN=At3g07010 PE=2 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 2.467e-11
Identity = 26/38 (68.42%), Postives = 31/38 (81.58%), Query Frame = 2 
Query:   65 GPQEKVNPKPGTLHHAVIQDKPLWIVFPRDMVIQLRKE 178
            G  + VNP PGTL HAVIQD+PLWI+F RDMVI L++E
Sbjct:  113 GDDDPVNPIPGTLRHAVIQDEPLWIIFKRDMVITLKQE 150

HSP 2 Score: 30.4166 bits (67), Expect = 2.467e-11
Identity = 12/14 (85.71%), Postives = 13/14 (92.86%), Query Frame = 1 
Query:   22 LGPRDGRFYVVTDP 63
            +G RDGRFYVVTDP
Sbjct:   99 IGGRDGRFYVVTDP 112
[Close]
BLAST of EY675161 vs. ExPASy Swiss-Prot
Match: PEL10_ARATH (Probable pectate lyase 10 OS=Arabidopsis thaliana GN=At3g24670 PE=2 SV=2)

HSP 1 Score: 59.3066 bits (142), Expect = 6.990e-11
Identity = 26/35 (74.29%), Postives = 30/35 (85.71%), Query Frame = 2 
Query:   74 EKVNPKPGTLHHAVIQDKPLWIVFPRDMVIQLRKE 178
            + VNP+PGTL HAVIQD+PLWIVF RDMVI L +E
Sbjct:  140 DAVNPRPGTLRHAVIQDRPLWIVFKRDMVITLTQE 174

HSP 2 Score: 29.261 bits (64), Expect = 6.990e-11
Identity = 11/14 (78.57%), Postives = 13/14 (92.86%), Query Frame = 1 
Query:   22 LGPRDGRFYVVTDP 63
            +G RDGR+YVVTDP
Sbjct:  123 IGGRDGRYYVVTDP 136
The following BLAST results are available for this feature:
BLAST of EY675161 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 4
Match NameE-valueIdentityDescription
PEL1_ARATH1.073e-1281.82Probable pectate lyase 1 OS=Arabidopsis thaliana G... [more]
PEL15_ARATH1.123e-1172.22Probable pectate lyase 15 OS=Arabidopsis thaliana ... [more]
PEL8_ARATH2.467e-1168.42Probable pectate lyase 8 OS=Arabidopsis thaliana G... [more]
PEL10_ARATH6.990e-1174.29Probable pectate lyase 10 OS=Arabidopsis thaliana ... [more]
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Properties
Property NameValue
Genbank descriptionCS00-C1-401-007-A02-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY675161 ID=EY675161; Name=EY675161; organism=Citrus sinensis; type=EST; length=983bp
CCACGCGTCCGAGAAATGCCATTAGGTCCCCGTGATGGTCGCTTCTATGT
TGTCACTGACCCAAGGGCCCCAAGAAAAAGTTAACCCCAAACCTGGAACT
TTACACCATGCTGTTATCCAGGATAAACCTCTCTGGATTGTTTTCCCAAG
GGACATGGTGATTCAATTGAGAAAAGAAACTGATTGTCACATACTTCAGG
AGCATATAATGGACGAGGGGAGCAAGAGAAGATTGGTAAAGGAAAGTGCA
ACAAGAGACAAATGGAAACAAAAGAGATCGGACAAAAAGATCGAAGTAAA
GGAAGTAAAGAAAATAGTAGTGAAAAGAAAAGAGAAACAACGAAAACAAT
ACACACGGAACGAAACCCGAAAAGGAGCCGGAACAATAACTCCAACGAAT
CAAGACACAACAAAAATGAAAAAGAAGTCGAGAGAGAGGAGAAGAAGAAA
AGAAAACAAGAGACAAAAAAAGAGAGAGAGCGAAACGAAGAGAAAAAAAA
AAAAGAACGAAAGAGAGACGACGAAAGACGAAAAAAAGGAAGAGAAAAGA
GGAAACAGAAAGAACAAAAACGAAAGGAAAAAAACAAGCAAGAGAGAAAG
AGACACGAAGAAGCAGAACAGGAAAGGATGAGAGAAAAAAGAGGAAAACA
AAGAGACAAAGGAAGAGCAAAGAAAAAGGAGAAAGAAGAAAAGAGAAGAA
AGAAAAAAGAAGAGAAACAAAAACGAAGAGGCAACAAGAGAGAGGAAAAA
ACAAAGAAAACGAAAGGAAAAGAAACGGCAGCAAAAAAGGAAGAAAGACA
GGAGAGAAGAACGAAAGCAAAGAAAAAGGAGAACGGCGGCAAAAAAAGAG
AAGGAGGGACAGGAAGAACGGAGAGAGAAAAAAGAAAAAAAAAAAGAGAG
ACAATAAAAGAAGAAAAATGGAGAGCAACAAGAGAGAAAAAAGACAGAGC
AAAGAAGAGAAGAGAAAAGAAAAAGAAAAAAAA
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