EY674779

Overview
NameEY674779
Unique NameEY674779
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length1025
Libraries
Library NameType
Sweet orange leaf, infected with CiLVcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY674779 vs. ExPASy Swiss-Prot
Match: MAON_SOLTU (NAD-dependent malic enzyme 59 kDa isoform, mitochondrial OS=Solanum tuberosum PE=1 SV=1)

HSP 1 Score: 218.779 bits (556), Expect = 4.127e-56
Identity = 119/189 (62.96%), Postives = 137/189 (72.49%), Query Frame = 2 
Query:    2 YTPTVALVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPAQQVDMIVLTDGSRILGLGDLGVQGIGIPIGKLDVYVAAAGINPQRILPVMLDVGTNNQKLLEDRLFEQIWDSDNLG*KEKNI----YQLLMNSWKLFTHV-GPKLLYSSRTSK*SGLFELWNDIEKGFVCLMMTYRGTAGVALAGIIG 553
            YTPTV LVCQNYSGLFRRPRGMYFSAKDKGEMMSMI+NWP+ QVDMIVLTDGSRILGLGDLGVQGIGIPIGKLD+YVAAAGINPQR+LPVMLDVGTNNQKLLED L+        LG ++  +    Y  +++ +    H   PK +      +    FE  +   K F       +GTAGVALAG++G
Sbjct:  129 YTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIFNWPSTQVDMIVLTDGSRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRVLPVMLDVGTNNQKLLEDPLY--------LGLRQPRLEGEEYLSIVDEFVEAVHARWPKAVVQFEDFQAKWAFETLDRYRKKFCMFNDDIQGTAGVALAGLLG 309
[Close]
BLAST of EY674779 vs. ExPASy Swiss-Prot
Match: MAOM_SOLTU (NAD-dependent malic enzyme 62 kDa isoform, mitochondrial OS=Solanum tuberosum PE=1 SV=1)

HSP 1 Score: 202.216 bits (513), Expect = 3.998e-51
Identity = 106/184 (57.61%), Postives = 132/184 (71.74%), Query Frame = 2 
Query:    2 YTPTVALVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPAQQVDMIVLTDGSRILGLGDLGVQGIGIPIGKLDVYVAAAGINPQRILPVMLDVGTNNQKLLEDRLFEQIWDSDNLG*KEKNIYQLLMNSWKLFTHVGPKLLYSSRTSK*SGLFELWNDIEKGFVCLMMTYRGTAGVALAGIIG 553
            YTPTV LVCQ YSGLFRRPRGMYFSA+D+GEMMSM+YNWPA QVDMIV+TDGSRILGLGDLG+QGIGI IGKLD+YVAAAGINPQR+LPVM+DVGT+N+ LL+D L+  + D    G +   +    M +  +FT   P ++      +    F+L       +       +GTAGVA+AG++G
Sbjct:  146 YTPTVGLVCQKYSGLFRRPRGMYFSAEDRGEMMSMVYNWPADQVDMIVVTDGSRILGLGDLGIQGIGIAIGKLDLYVAAAGINPQRVLPVMIDVGTDNENLLKDPLYLGLQDHRLDGEEYIEVIDEFMEA--VFTR-WPHVIVQFEDFQSKWAFKLLQRYRNNYRMFNDDIQGTAGVAIAGLLG 326
[Close]
BLAST of EY674779 vs. ExPASy Swiss-Prot
Match: MAOH_NEOFR (Malic enzyme, hydrogenosomal OS=Neocallimastix frontalis PE=1 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 2.103e-47
Identity = 62/107 (57.94%), Postives = 80/107 (74.77%), Query Frame = 2 
Query:    2 YTPTVALVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPAQQVDMIVLTDGSRILGLGDLGVQGIGIPIGKLDVYVAAAGINPQRILPVMLDVGTNNQKLLEDRLF 322
            YTP V   CQ +  +F + RGMYFS  D+G+M ++  NWP   VD+IV+TDGSRILGLGDLG  G+ IPIGKL +YV   GINP+ +LP++LDVGTNN++LL D L+
Sbjct:  134 YTPVVGEACQKFHKIFTQTRGMYFSTADRGQMSAVAANWPYDDVDVIVVTDGSRILGLGDLGAGGMQIPIGKLTLYVCGGGINPRNVLPIVLDVGTNNKELLNDPLY 240

HSP 2 Score: 74.3294 bits (181), Expect = 2.103e-47
Identity = 39/104 (37.50%), Postives = 64/104 (61.54%), Query Frame = 1 
Query:  331 LGLRQPRLEGEEYLSIVDEFMEAVHARWPKAIVQFEDFQMKWAF*TLERYRKRFCMFNDDLQGNCWCCTCGNYWELSEHKGLSLTDLLRNQKKFVLGGWGTPGL 642
            LG++ PRL+GEE+ + VDE++ A+  R+PKA++QFEDF M  A   L +Y+ + CMFNDD+Q +    T  +       +G +  D+   ++ F+  G G+ G+
Sbjct:  241 LGMQHPRLQGEEFHAFVDEWVSAITDRFPKAVIQFEDFMMPNALDLLLKYKDQICMFNDDIQ-STGAITLASVLATMRARGGTFADI--KKETFLCLGAGSSGV 341
[Close]
BLAST of EY674779 vs. ExPASy Swiss-Prot
Match: MAOM_AMAHP (NAD-dependent malic enzyme 65 kDa isoform, mitochondrial OS=Amaranthus hypochondriacus PE=1 SV=1)

HSP 1 Score: 189.119 bits (479), Expect = 3.502e-47
Identity = 101/189 (53.44%), Postives = 133/189 (70.37%), Query Frame = 2 
Query:    5 TPTVALVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPAQQVDMIVLTDGSRILGLGDLGVQGIGIPIGKLDVYVAAAGINPQRILPVMLDVGTNNQKLLEDRLFEQIWDSDNLG*KEKNI----YQLLMNSW--KLFTHVGPKLLYSSRTSK*SGLFELWNDIEKGFVCLMMTYRGTAGVALAGIIG 553
            TPTV LVCQ +SGL+RRPRGMYFS+ D+GEMMSM+YNWPA+QVDMIV+TDGSRILGLGDLGV GIG+ IGKLD+YVAAAGINPQR+LPVM+DVGTNN+ LL++ L+        LG ++K +    Y  +M+ +   +FT   P ++      +      L       +    +  +GT+GVA+AG++G
Sbjct:  144 TPTVGLVCQKFSGLYRRPRGMYFSSDDRGEMMSMVYNWPAEQVDMIVVTDGSRILGLGDLGVHGIGVAIGKLDLYVAAAGINPQRVLPVMIDVGTNNEDLLKNPLY--------LGLQKKRLDGEEYLAVMDEFMEAVFTR-WPNVIVQFEDIQNKWALTLLQRYRHKYRTFNVDVQGTSGVAIAGLLG 323
[Close]
BLAST of EY674779 vs. ExPASy Swiss-Prot
Match: MAO1_ERWT9 (NAD-dependent malic enzyme OS=Erwinia tasmaniensis (strain DSM 17950 / Et1/99) GN=sfcA PE=3 SV=1)

HSP 1 Score: 122.479 bits (306), Expect = 9.305e-45
Identity = 57/107 (53.27%), Postives = 77/107 (71.96%), Query Frame = 2 
Query:    2 YTPTVALVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPAQQVDMIVLTDGSRILGLGDLGVQGIGIPIGKLDVYVAAAGINPQRILPVMLDVGTNNQKLLEDRLF 322
            YTPTV   C+++S ++RR RG++ S  ++  +  M+ N   Q V +IV+TDG RILGLGD G+ G+GIPIGKL +Y A  GI+P   LPV+LDVGTNNQ+LL D L+
Sbjct:  104 YTPTVGAACEHFSEIYRRARGLFISYPNRANIEDMLQNATKQNVKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLY 210

HSP 2 Score: 80.1073 bits (196), Expect = 9.305e-45
Identity = 45/103 (43.69%), Postives = 58/103 (56.31%), Query Frame = 1 
Query:  331 LGLRQPRLEGEEYLSIVDEFMEAVHARWPKAIVQFEDFQMKWAF*TLERYRKRFCMFNDDLQGNCWCCTCGNYWELSEHKGLSLTDLLRNQKKFVLGGWGTPG 639
            +G R PR+ GEEY + V+EF++AV +RWP  ++QFEDF  K A   LERYR   C FNDD+QG     T G     S   G  L      ++K V  G G+ G
Sbjct:  211 MGWRHPRITGEEYDNFVNEFIQAVKSRWPNVLLQFEDFAQKNAMPLLERYRDEVCCFNDDIQGTA-AVTLGTLIAASRAAGGKLC-----EQKVVFLGAGSAG 307
[Close]
BLAST of EY674779 vs. ExPASy Swiss-Prot
Match: MAO1_SHISS (NAD-dependent malic enzyme OS=Shigella sonnei (strain Ss046) GN=sfcA PE=3 SV=2)

HSP 1 Score: 121.709 bits (304), Expect = 2.684e-44
Identity = 56/107 (52.34%), Postives = 77/107 (71.96%), Query Frame = 2 
Query:    2 YTPTVALVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPAQQVDMIVLTDGSRILGLGDLGVQGIGIPIGKLDVYVAAAGINPQRILPVMLDVGTNNQKLLEDRLF 322
            YTPTV   C+ +S ++RR RG++ S +++  M  ++ N P   + +IV+TDG RILGLGD G+ G+GIPIGKL +Y A  GI+P   LPV+LDVGTNNQ+LL D L+
Sbjct:  104 YTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLY 210

HSP 2 Score: 79.337 bits (194), Expect = 2.684e-44
Identity = 44/103 (42.72%), Postives = 55/103 (53.40%), Query Frame = 1 
Query:  331 LGLRQPRLEGEEYLSIVDEFMEAVHARWPKAIVQFEDFQMKWAF*TLERYRKRFCMFNDDLQGNCWCCTCGNYWELSEHKGLSLTDLLRNQKKFVLGGWGTPG 639
            +G R PR+  +EY   VDEF++AV  RWP  ++QFEDF  K A   L RYR   C FNDD+QG     T G     S   G  L     ++KK V  G G+ G
Sbjct:  211 MGWRNPRITDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTA-AVTVGTLIAASRAAGGQL-----SEKKIVFLGAGSAG 307
[Close]
BLAST of EY674779 vs. ExPASy Swiss-Prot
Match: MAO1_SHIFL (NAD-dependent malic enzyme OS=Shigella flexneri GN=sfcA PE=3 SV=4)

HSP 1 Score: 121.709 bits (304), Expect = 2.684e-44
Identity = 56/107 (52.34%), Postives = 77/107 (71.96%), Query Frame = 2 
Query:    2 YTPTVALVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPAQQVDMIVLTDGSRILGLGDLGVQGIGIPIGKLDVYVAAAGINPQRILPVMLDVGTNNQKLLEDRLF 322
            YTPTV   C+ +S ++RR RG++ S +++  M  ++ N P   + +IV+TDG RILGLGD G+ G+GIPIGKL +Y A  GI+P   LPV+LDVGTNNQ+LL D L+
Sbjct:  104 YTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLY 210

HSP 2 Score: 79.337 bits (194), Expect = 2.684e-44
Identity = 44/103 (42.72%), Postives = 55/103 (53.40%), Query Frame = 1 
Query:  331 LGLRQPRLEGEEYLSIVDEFMEAVHARWPKAIVQFEDFQMKWAF*TLERYRKRFCMFNDDLQGNCWCCTCGNYWELSEHKGLSLTDLLRNQKKFVLGGWGTPG 639
            +G R PR+  +EY   VDEF++AV  RWP  ++QFEDF  K A   L RYR   C FNDD+QG     T G     S   G  L     ++KK V  G G+ G
Sbjct:  211 MGWRNPRITDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTA-AVTVGTLIAASRAAGGQL-----SEKKIVFLGAGSAG 307
[Close]
BLAST of EY674779 vs. ExPASy Swiss-Prot
Match: MAO1_SHIF8 (NAD-dependent malic enzyme OS=Shigella flexneri serotype 5b (strain 8401) GN=sfcA PE=3 SV=1)

HSP 1 Score: 121.709 bits (304), Expect = 2.684e-44
Identity = 56/107 (52.34%), Postives = 77/107 (71.96%), Query Frame = 2 
Query:    2 YTPTVALVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPAQQVDMIVLTDGSRILGLGDLGVQGIGIPIGKLDVYVAAAGINPQRILPVMLDVGTNNQKLLEDRLF 322
            YTPTV   C+ +S ++RR RG++ S +++  M  ++ N P   + +IV+TDG RILGLGD G+ G+GIPIGKL +Y A  GI+P   LPV+LDVGTNNQ+LL D L+
Sbjct:  104 YTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLY 210

HSP 2 Score: 79.337 bits (194), Expect = 2.684e-44
Identity = 44/103 (42.72%), Postives = 55/103 (53.40%), Query Frame = 1 
Query:  331 LGLRQPRLEGEEYLSIVDEFMEAVHARWPKAIVQFEDFQMKWAF*TLERYRKRFCMFNDDLQGNCWCCTCGNYWELSEHKGLSLTDLLRNQKKFVLGGWGTPG 639
            +G R PR+  +EY   VDEF++AV  RWP  ++QFEDF  K A   L RYR   C FNDD+QG     T G     S   G  L     ++KK V  G G+ G
Sbjct:  211 MGWRNPRITDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTA-AVTVGTLIAASRAAGGQL-----SEKKIVFLGAGSAG 307
[Close]
BLAST of EY674779 vs. ExPASy Swiss-Prot
Match: MAO1_ESCF3 (NAD-dependent malic enzyme OS=Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73) GN=sfcA PE=3 SV=1)

HSP 1 Score: 121.709 bits (304), Expect = 2.684e-44
Identity = 56/107 (52.34%), Postives = 77/107 (71.96%), Query Frame = 2 
Query:    2 YTPTVALVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPAQQVDMIVLTDGSRILGLGDLGVQGIGIPIGKLDVYVAAAGINPQRILPVMLDVGTNNQKLLEDRLF 322
            YTPTV   C+ +S ++RR RG++ S +++  M  ++ N P   + +IV+TDG RILGLGD G+ G+GIPIGKL +Y A  GI+P   LPV+LDVGTNNQ+LL D L+
Sbjct:  104 YTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLY 210

HSP 2 Score: 79.337 bits (194), Expect = 2.684e-44
Identity = 44/103 (42.72%), Postives = 55/103 (53.40%), Query Frame = 1 
Query:  331 LGLRQPRLEGEEYLSIVDEFMEAVHARWPKAIVQFEDFQMKWAF*TLERYRKRFCMFNDDLQGNCWCCTCGNYWELSEHKGLSLTDLLRNQKKFVLGGWGTPG 639
            +G R PR+  +EY   VDEF++AV  RWP  ++QFEDF  K A   L RYR   C FNDD+QG     T G     S   G  L     ++KK V  G G+ G
Sbjct:  211 MGWRNPRITDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTA-AVTVGTLIAASRAAGGQL-----SEKKIVFLGAGSAG 307
[Close]
BLAST of EY674779 vs. ExPASy Swiss-Prot
Match: MAO1_ECOUT (NAD-dependent malic enzyme OS=Escherichia coli (strain UTI89 / UPEC) GN=sfcA PE=3 SV=2)

HSP 1 Score: 121.709 bits (304), Expect = 2.684e-44
Identity = 56/107 (52.34%), Postives = 77/107 (71.96%), Query Frame = 2 
Query:    2 YTPTVALVCQNYSGLFRRPRGMYFSAKDKGEMMSMIYNWPAQQVDMIVLTDGSRILGLGDLGVQGIGIPIGKLDVYVAAAGINPQRILPVMLDVGTNNQKLLEDRLF 322
            YTPTV   C+ +S ++RR RG++ S +++  M  ++ N P   + +IV+TDG RILGLGD G+ G+GIPIGKL +Y A  GI+P   LPV+LDVGTNNQ+LL D L+
Sbjct:  104 YTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLY 210

HSP 2 Score: 79.337 bits (194), Expect = 2.684e-44
Identity = 44/103 (42.72%), Postives = 55/103 (53.40%), Query Frame = 1 
Query:  331 LGLRQPRLEGEEYLSIVDEFMEAVHARWPKAIVQFEDFQMKWAF*TLERYRKRFCMFNDDLQGNCWCCTCGNYWELSEHKGLSLTDLLRNQKKFVLGGWGTPG 639
            +G R PR+  +EY   VDEF++AV  RWP  ++QFEDF  K A   L RYR   C FNDD+QG     T G     S   G  L     ++KK V  G G+ G
Sbjct:  211 MGWRNPRITDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTA-AVTVGTLIAASRAAGGQL-----SEKKIVFLGAGSAG 307
The following BLAST results are available for this feature:
BLAST of EY674779 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 140
Match NameE-valueIdentityDescription
MAON_SOLTU4.127e-5662.96NAD-dependent malic enzyme 59 kDa isoform, mitocho... [more]
MAOM_SOLTU3.998e-5157.61NAD-dependent malic enzyme 62 kDa isoform, mitocho... [more]
MAOH_NEOFR2.103e-4757.94Malic enzyme, hydrogenosomal OS=Neocallimastix fro... [more]
MAOM_AMAHP3.502e-4753.44NAD-dependent malic enzyme 65 kDa isoform, mitocho... [more]
MAO1_ERWT99.305e-4553.27NAD-dependent malic enzyme OS=Erwinia tasmaniensis... [more]
MAO1_SHISS2.684e-4452.34NAD-dependent malic enzyme OS=Shigella sonnei (str... [more]
MAO1_SHIFL2.684e-4452.34NAD-dependent malic enzyme OS=Shigella flexneri GN... [more]
MAO1_SHIF82.684e-4452.34NAD-dependent malic enzyme OS=Shigella flexneri se... [more]
MAO1_ESCF32.684e-4452.34NAD-dependent malic enzyme OS=Escherichia ferguson... [more]
MAO1_ECOUT2.684e-4452.34NAD-dependent malic enzyme OS=Escherichia coli (st... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-401-001-C12-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY674779 ID=EY674779; Name=EY674779; organism=Citrus sinensis; type=EST; length=1025bp
ATACACTCCAACTGTTGCATTGGTTTGCCAGAATTATTCAGGTTTGTTTA
GACGTCCCCGTGGAATGTATTTCAGTGCAAAGGATAAGGGGGAGATGATG
TCTATGATCTACAATTGGCCTGCTCAACAGGTAGATATGATAGTCCTAAC
GGATGGAAGTCGTATTCTTGGCTTAGGCGACCTTGGAGTTCAGGGAATTG
GAATTCCTATTGGAAAGCTGGATGTGTATGTGGCTGCTGCAGGCATCAAT
CCACAGAGAATTCTCCCGGTTATGCTTGATGTTGGCACTAACAATCAAAA
GCTGCTTGAAGACCGTCTATTTGAACAGATTTGGGACTCCGACAACCTAG
GCTAGAAGGAGAAGAATATCTATCAATTGTTGATGAATTCATGGAAGCTG
TTCACGCACGTTGGCCCAAAGCTATTGTACAGTTCGAGGACTTCCAAATG
AAGTGGGCTTTTTGAACTTTGGAACGATATCGAAAAAGGTTTTGTATGTT
TAATGATGACTTACAGGGGAACTGCTGGTGTTGCACTTGCGGGAATTATT
GGGAACTGTCAGAACACAAGGGTCTATCATTGACTGACCTTTTGCGGAAC
CAAAAAAAATTTGTATTGGGGGGCTGGGGAACCCCCGGGCTTTGGTGGTT
CCTAAAATGGCTTGTACAACCCTGCACCAAAGAAATGGCCGGGGAAAAAA
TTGGACCCCTTTTTGCCAAAAATTAAATTTTTTCCCTTCTTTTGACAAAA
AATGGGCCTCTCCCCCCAAAAAAAAAAGGGAAAAAATCTTTGGCCCCACC
GCCCCGCCCCTTTTTTGCTAAAAAATCCCCAAGAAATTTTTTGGGGGGGT
TTATGGGAGGGGGGACCTAAGCCTTATTTTTGAGGTGGGGGTAAAAAAAG
GAGGACAAACCCCCCCAGTTGTGGCTTCCTTTGGGGGTTATTTTCGCTGG
GGGGGCAAATGAGGTGGGGGGGTTGCTCTTTCTCCTCTAGAAGAAAGAAA
AAAAAAGAACAAAAATAGGGGAGAA
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