EY674772

Overview
NameEY674772
Unique NameEY674772
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length1108
Libraries
Library NameType
Sweet orange leaf, infected with CiLVcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY674772 vs. ExPASy Swiss-Prot
Match: LGUL_ORYSJ (Lactoylglutathione lyase OS=Oryza sativa subsp. japonica GN=GLX-I PE=1 SV=2)

HSP 1 Score: 145.976 bits (367), Expect = 3.827e-34
Identity = 83/164 (50.61%), Postives = 95/164 (57.93%), Query Frame = 2 
Query:   74 WPKKDKRRLLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELDVQLWCNFI*YWNWFWPFCNCN*RCLQTG*KHSCQGLAMSLESLVQLKVEQLTLPL*TIPMVIFLKLSQRGPTPETLRPVLLRVGKLGRSLKFYENGFGGK 565
            WPKKDK+RLLHAVYRVGDLDRTIK YTECFGMKLLRKRDVPEEKY+NAFLGFGPE ++F +EL      +       F  F        +   K               +K     +     P     +L QRGPTPE L  V+LRVG L RS+KFYE   G K
Sbjct:   17 WPKKDKKRLLHAVYRVGDLDRTIKCYTECFGMKLLRKRDVPEEKYTNAFLGFGPEDTNFALELTYNYGVDKYDIGAGFGHFAIATEDVYKLAEKIKSSCCCKITREPGPVKGGSTVIAFAQDPDGYMFELIQRGPTPEPLCQVMLRVGDLDRSIKFYEKALGMK 180
[Close]
BLAST of EY674772 vs. ExPASy Swiss-Prot
Match: LGUL_BRAOG (Putative lactoylglutathione lyase OS=Brassica oleracea var. gemmifera PE=2 SV=1)

HSP 1 Score: 145.206 bits (365), Expect = 6.527e-34
Identity = 91/187 (48.66%), Postives = 111/187 (59.36%), Query Frame = 2 
Query:   74 WPKKDKRRLLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELDVQLWCNFI*YWNWFWPFCNCN*RCLQTG*KHSCQGLAMSLESLVQLKVEQLTLPL*TI----PMVIFLK--------LSQRGPTPETLRPVLLRVGKLGRSLKFYENGFGGKNW*GTVEKPEY 598
            WPKKDKRR LH VYRVGDLDRTI+FYTECFGMK+LRKRDVPEEKYSNAFLGFGPE S+FVVEL      +       F  F              S Q ++  +E+ V+ K   +T     +     ++ F+K        L QRGPTPE L  V+LRVG L R++KF E   G +     +E+PEY
Sbjct:   10 WPKKDKRRFLHVVYRVGDLDRTIQFYTECFGMKVLRKRDVPEEKYSNAFLGFGPETSNFVVELTYNYGVSSYDIGTGFGHFA------------ISTQDVSKMVEA-VRAKGGNVTREPGPVKGGGSVIAFVKDPDGYTFELIQRGPTPEPLCQVMLRVGDLDRAVKFMEKALGMRLL-RRIERPEY 182

HSP 2 Score: 42.743 bits (99), Expect = 1.083e-11
Identity = 22/55 (40.00%), Postives = 32/55 (58.18%), Query Frame = 2 
Query:   98 LLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVEL 262
            L   + RVGDLDR +KF  +  GM+LLR+ + PE   +   +G+  E    V+EL
Sbjct:  148 LCQVMLRVGDLDRAVKFMEKALGMRLLRRIERPEYN-TIGMMGYAEEYESIVLEL 201

HSP 3 Score: 40.817 bits (94), Expect = 1.083e-11
Identity = 17/33 (51.52%), Postives = 21/33 (63.64%), Query Frame = 3 
Query:  264 TYNYGVTSYDIGTGFGHFAIATEDVYKLVENIR 362
            TYNYGVT Y  G  +   AI T+DVYK  E ++
Sbjct:  202 TYNYGVTEYTKGNAYAQIAIGTDDVYKSAEVVK 234

HSP 4 Score: 27.335 bits (59), Expect = 1.083e-11
Identity = 12/26 (46.15%), Postives = 15/26 (57.69%), Query Frame = 1 
Query:  373 GNVTREPGPVKSGTTHIAFVNDPDGY 450
            G +TRE GP+    T I    DPDG+
Sbjct:  242 GKITREAGPLPGLGTKIVSFLDPDGW 267
[Close]
BLAST of EY674772 vs. ExPASy Swiss-Prot
Match: LGUL_VIBPA (Probable lactoylglutathione lyase OS=Vibrio parahaemolyticus GN=gloA PE=3 SV=2)

HSP 1 Score: 65.0846 bits (157), Expect = 5.248e-29
Identity = 30/56 (53.57%), Postives = 41/56 (73.21%), Query Frame = 2 
Query:   95 RLLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVEL 262
            R+LH + RVGDLD++IKFYTE  GM+LLR  +  E +Y+ AF+G+G E    V+EL
Sbjct:    5 RILHTMLRVGDLDKSIKFYTEVMGMQLLRTNENKEYEYTLAFVGYGDESQGAVIEL 60

HSP 2 Score: 56.9954 bits (136), Expect = 5.248e-29
Identity = 25/37 (67.57%), Postives = 28/37 (75.68%), Query Frame = 1 
Query:  373 GNVTREPGPVKSGTTHIAFVNDPDGYIFETLPERSNS 483
            GNVTRE GPVK GTTHIAFV DPDGY+ E +  +  S
Sbjct:   97 GNVTREAGPVKGGTTHIAFVKDPDGYMIELIQNKQAS 133

HSP 3 Score: 48.1358 bits (113), Expect = 5.248e-29
Identity = 19/36 (52.78%), Postives = 25/36 (69.44%), Query Frame = 3 
Query:  264 TYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKG 371
            TYN+G T YD+GT FGH AI  +D+Y   + I+A G
Sbjct:   61 TYNWGKTEYDLGTAFGHIAIGVDDIYATCDAIKAAG 96
[Close]
BLAST of EY674772 vs. ExPASy Swiss-Prot
Match: LGUL_SYNY3 (Probable lactoylglutathione lyase OS=Synechocystis sp. (strain PCC 6803) GN=gloA PE=3 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 7.912e-26
Identity = 27/55 (49.09%), Postives = 41/55 (74.55%), Query Frame = 2 
Query:   98 LLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVEL 262
            LLH + RVGDLD++++FY +  GM LLRK+D P  +++ AF+G+G E  + V+EL
Sbjct:    3 LLHTMIRVGDLDKSLQFYCDILGMNLLRKKDYPSGEFTLAFVGYGKESENAVIEL 57

HSP 2 Score: 47.7506 bits (112), Expect = 7.912e-26
Identity = 18/36 (50.00%), Postives = 24/36 (66.67%), Query Frame = 3 
Query:  264 TYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKG 371
            T+N+G   YD+G GFGH A+  ED+Y   + IR KG
Sbjct:   58 THNWGTDKYDLGNGFGHIALGVEDIYSTCDKIRDKG 93

HSP 3 Score: 47.7506 bits (112), Expect = 7.912e-26
Identity = 22/35 (62.86%), Postives = 24/35 (68.57%), Query Frame = 1 
Query:  373 GNVTREPGPVKSGTTHIAFVNDPDGYIFETLPERS 477
            G V REPGP+K GTT IAFV DPDGY  E +   S
Sbjct:   94 GKVVREPGPMKHGTTVIAFVEDPDGYKIELIQTSS 128
[Close]
BLAST of EY674772 vs. ExPASy Swiss-Prot
Match: LGUL_HAEIN (Lactoylglutathione lyase OS=Haemophilus influenzae GN=gloA PE=1 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 3.762e-25
Identity = 29/56 (51.79%), Postives = 40/56 (71.43%), Query Frame = 2 
Query:   95 RLLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVEL 262
            ++LH + RVGDLDR+IKFY +  GM+LLR  + PE KY+ AFLG+   +S   +EL
Sbjct:    2 QILHTMLRVGDLDRSIKFYQDVLGMRLLRTSENPEYKYTLAFLGYEDGESAAEIEL 57

HSP 2 Score: 50.0618 bits (118), Expect = 3.762e-25
Identity = 23/35 (65.71%), Postives = 26/35 (74.29%), Query Frame = 1 
Query:  373 GNVTREPGPVKSGTTHIAFVNDPDGYIFETLPERS 477
            GNVTRE GPVK G+T IAFV DPDGY  E +  +S
Sbjct:   94 GNVTREAGPVKGGSTVIAFVEDPDGYKIEFIENKS 128

HSP 3 Score: 45.8246 bits (107), Expect = 3.762e-25
Identity = 18/36 (50.00%), Postives = 24/36 (66.67%), Query Frame = 3 
Query:  264 TYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKG 371
            TYN+GV  Y+ GT +GH AI  +D+Y   E +RA G
Sbjct:   58 TYNWGVDKYEHGTAYGHIAIGVDDIYATCEAVRASG 93
[Close]
BLAST of EY674772 vs. ExPASy Swiss-Prot
Match: LGUL_SHIFL (Lactoylglutathione lyase OS=Shigella flexneri GN=gloA PE=3 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 3.596e-21
Identity = 34/56 (60.71%), Postives = 41/56 (73.21%), Query Frame = 2 
Query:   95 RLLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVEL 262
            RLLH + RVGDL R+I FYT+  GMKLLR  + PE KYS AF+G+GPE    V+EL
Sbjct:    2 RLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIEL 57

HSP 2 Score: 52.373 bits (124), Expect = 3.596e-21
Identity = 24/34 (70.59%), Postives = 26/34 (76.47%), Query Frame = 1 
Query:  373 GNVTREPGPVKSGTTHIAFVNDPDGYIFETLPER 474
            GNVTRE GPVK GTT IAFV DPDGY  E + E+
Sbjct:   94 GNVTREAGPVKGGTTVIAFVEDPDGYKIELIEEK 127
[Close]
BLAST of EY674772 vs. ExPASy Swiss-Prot
Match: LGUL_ECOLI (Lactoylglutathione lyase OS=Escherichia coli (strain K12) GN=gloA PE=1 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 3.596e-21
Identity = 34/56 (60.71%), Postives = 41/56 (73.21%), Query Frame = 2 
Query:   95 RLLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVEL 262
            RLLH + RVGDL R+I FYT+  GMKLLR  + PE KYS AF+G+GPE    V+EL
Sbjct:    2 RLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIEL 57

HSP 2 Score: 52.373 bits (124), Expect = 3.596e-21
Identity = 24/34 (70.59%), Postives = 26/34 (76.47%), Query Frame = 1 
Query:  373 GNVTREPGPVKSGTTHIAFVNDPDGYIFETLPER 474
            GNVTRE GPVK GTT IAFV DPDGY  E + E+
Sbjct:   94 GNVTREAGPVKGGTTVIAFVEDPDGYKIELIEEK 127
[Close]
BLAST of EY674772 vs. ExPASy Swiss-Prot
Match: LGUL_ECO57 (Lactoylglutathione lyase OS=Escherichia coli O157:H7 GN=gloA PE=3 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 3.596e-21
Identity = 34/56 (60.71%), Postives = 41/56 (73.21%), Query Frame = 2 
Query:   95 RLLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVEL 262
            RLLH + RVGDL R+I FYT+  GMKLLR  + PE KYS AF+G+GPE    V+EL
Sbjct:    2 RLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIEL 57

HSP 2 Score: 52.373 bits (124), Expect = 3.596e-21
Identity = 24/34 (70.59%), Postives = 26/34 (76.47%), Query Frame = 1 
Query:  373 GNVTREPGPVKSGTTHIAFVNDPDGYIFETLPER 474
            GNVTRE GPVK GTT IAFV DPDGY  E + E+
Sbjct:   94 GNVTREAGPVKGGTTVIAFVEDPDGYKIELIEEK 127
[Close]
BLAST of EY674772 vs. ExPASy Swiss-Prot
Match: LGUL_VIBCH (Probable lactoylglutathione lyase OS=Vibrio cholerae GN=gloA PE=3 SV=2)

HSP 1 Score: 67.0106 bits (162), Expect = 6.071e-21
Identity = 31/56 (55.36%), Postives = 41/56 (73.21%), Query Frame = 2 
Query:   95 RLLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVEL 262
            R+LH + RVGDLD++I+FYT+  GM LLRK +  E KY+ AFLG+G E    V+EL
Sbjct:    5 RILHTMLRVGDLDKSIEFYTQVMGMSLLRKNENTEYKYTLAFLGYGDESQGAVIEL 60

HSP 2 Score: 55.8398 bits (133), Expect = 6.071e-21
Identity = 24/34 (70.59%), Postives = 27/34 (79.41%), Query Frame = 1 
Query:  373 GNVTREPGPVKSGTTHIAFVNDPDGYIFETLPER 474
            G VTREPGPVK GTTHIAFV DPDGY+ E +  +
Sbjct:   97 GIVTREPGPVKGGTTHIAFVKDPDGYMIELIQNK 130
[Close]
BLAST of EY674772 vs. ExPASy Swiss-Prot
Match: LGUL_SALTY (Lactoylglutathione lyase OS=Salmonella typhimurium GN=gloA PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 6.498e-20
Identity = 34/56 (60.71%), Postives = 40/56 (71.43%), Query Frame = 2 
Query:   95 RLLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVEL 262
            RLLH + RVGDL R+I FYT   GMKLLR  + PE KYS AF+G+GPE    V+EL
Sbjct:    2 RLLHTMLRVGDLQRSIAFYTNVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIEL 57

HSP 2 Score: 48.521 bits (114), Expect = 6.498e-20
Identity = 22/31 (70.97%), Postives = 24/31 (77.42%), Query Frame = 1 
Query:  373 GNVTREPGPVKSGTTHIAFVNDPDGYIFETL 465
            GNVTRE GPVK G+T IAFV DPDGY  E +
Sbjct:   94 GNVTREAGPVKGGSTIIAFVEDPDGYKIELI 124
The following BLAST results are available for this feature:
BLAST of EY674772 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 13
Match NameE-valueIdentityDescription
LGUL_ORYSJ3.827e-3450.61Lactoylglutathione lyase OS=Oryza sativa subsp. ja... [more]
LGUL_BRAOG6.527e-3448.66Putative lactoylglutathione lyase OS=Brassica oler... [more]
LGUL_VIBPA5.248e-2953.57Probable lactoylglutathione lyase OS=Vibrio paraha... [more]
LGUL_SYNY37.912e-2649.09Probable lactoylglutathione lyase OS=Synechocystis... [more]
LGUL_HAEIN3.762e-2551.79Lactoylglutathione lyase OS=Haemophilus influenzae... [more]
LGUL_SHIFL3.596e-2160.71Lactoylglutathione lyase OS=Shigella flexneri GN=g... [more]
LGUL_ECOLI3.596e-2160.71Lactoylglutathione lyase OS=Escherichia coli (stra... [more]
LGUL_ECO573.596e-2160.71Lactoylglutathione lyase OS=Escherichia coli O157:... [more]
LGUL_VIBCH6.071e-2155.36Probable lactoylglutathione lyase OS=Vibrio choler... [more]
LGUL_SALTY6.498e-2060.71Lactoylglutathione lyase OS=Salmonella typhimurium... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionCS00-C1-401-001-C04-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY674772 ID=EY674772; Name=EY674772; organism=Citrus sinensis; type=EST; length=1108bp
CCGACTTTCTGACCCATTCCATATCAAAATGGCTGAGGCTTCTCCTGCTG
CTGCTCATGCCGAGTTGCTGGAATGGCCAAAGAAAGATAAGCGACGCCTC
CTTCATGCTGTGTATCGTGTTGGTGATCTTGATCGCACCATCAAATTTTA
CACCGAATGCTTTGGGATGAAGCTGTTGAGAAAGAGAGACGTCCCGGAAG
AGAAATACTCAAATGCCTTTCTTGGATTTGGCCCCGAACAGTCTCACTTT
GTTGTGGAGTTAGACGTACAACTATGGTGTAACTTCATATGATATTGGAA
CTGGTTTTGGCCATTTTGCAATTGCAACTGAAGATGTTTACAAACTGGTT
GAAAACATTCGTGCCAAGGGTTGGCAATGTCACTAGAGAGCCTGGTCCAG
TTAAAAGTGGAACAACTCACATTGCCTTTGTGAACGATCCCGATGGTTAT
ATTTTTGAAACTCTCCCAGAGAGGTCCAACTCCTGAAACACTCCGTCCAG
TATTGCTTCGCGTTGGCAAACTTGGTCGCTCCCTCAAATTTTATGAAAAT
GGTTTTGGGGGAAAAAACTGGTAAGGGACAGTTGAAAAACCTGAATACCA
ATTACACCCTTGGCCCTGGCTTTGGAATTTCCTGAGAAAAAATAAAAACA
ACTGTCTCGGAATTTAAACTTTTAATTAATGGGTGTGTCTTGAATAATCC
CCAAGGGAAAATGGAATTTGCCCCCAGGTTTTCCAAAAAACAAACTGAAA
AAGGATAAAAAAAAATGGTGTAAAAGGTGGACAAACCATGGTATCACCAA
GAAACCTATGGGAGAGAATAATAAACTCTCTTGCAAACCCAAGATATTCA
TCCCCTTGGGATCATATTACAACACTAAATATATCCCCCCCCTTTTTATA
TGTAAACACCAACCAAGAAGGGGGGGGGAAAAAAAAAGTAGGTTCCTGGA
GGTGTTGTTAAATAATAAAAGAAAAATAAAAAATTTTTTTTTGCATGAAA
CAAAAATAAAGAATATTTTAATACACCCACCAAATATTCTTGCGTACGAA
TAAACTTCAATTATAAAAGAAAAAATGGTGGCAAAAAAAACTGACTTCGC
CACTACAT
back to top