EY702594

Overview
NameEY702594
Unique NameEY702594
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length868
Libraries
Library NameType
Sweet orange fruit, development stadium (2 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig1286 contig Csv1_Contig1286:503..1370. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of EY702594 vs. ExPASy Swiss-Prot
Match: GSH1_SOLLC (Glutamate--cysteine ligase, chloroplastic OS=Solanum lycopersicum GN=GSH1 PE=2 SV=1)

HSP 1 Score: 476.478 bits (1225), Expect = 8.592e-134
Identity = 234/278 (84.17%), Postives = 246/278 (88.49%), Query Frame = 1 
Query:   13 VGYLASGCKPKEKWRIGTEHEKFGFEFGTLHPMKYEQIAELVNSIAERFDWEKVMEGDYIIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVAKEMGIGFLGIGFQPKWGLKDIPVMPKGRYEIMRNYMPKVGSLGLDMMFRTCTVHVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRGHIWTDTDNNRAGMLPFAFDDSFGFEQYGDYALDGQ*NLLIAKRVY*LCWDV-IQGLLGWKIPCPPGELPLL 843
            VGYLASGCK KEKWRIGTEHEKFGFEFGTL PMKY+QIA+L+N IAERFDWEKVMEGD IIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVA+EMGIGFLG GFQPKWGLKDIP+MPKGRYEI+RNYMPKVGSLGLDMMFRTCTV VNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYLS R HIWTDTDNNRAGMLPF FDDSFGFEQY DYALD     +  K+ Y  C  +  +  +  K+P  PGE P L
Sbjct:   96 VGYLASGCKSKEKWRIGTEHEKFGFEFGTLRPMKYDQIADLLNGIAERFDWEKVMEGDKIIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGTGFQPKWGLKDIPIMPKGRYEIIRNYMPKVGSLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSKRSHIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYVDCTGLSFRDFMNGKLPPIPGEYPTL 373
[Close]
BLAST of EY702594 vs. ExPASy Swiss-Prot
Match: GSH1_TOBAC (Glutamate--cysteine ligase, chloroplastic OS=Nicotiana tabacum GN=GSH1 PE=2 SV=2)

HSP 1 Score: 476.093 bits (1224), Expect = 1.122e-133
Identity = 236/278 (84.89%), Postives = 245/278 (88.13%), Query Frame = 1 
Query:   13 VGYLASGCKPKEKWRIGTEHEKFGFEFGTLHPMKYEQIAELVNSIAERFDWEKVMEGDYIIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVAKEMGIGFLGIGFQPKWGLKDIPVMPKGRYEIMRNYMPKVGSLGLDMMFRTCTVHVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRGHIWTDTDNNRAGMLPFAFDDSFGFEQYGDYALDGQ*NLLIAKRVY*LCWDV-IQGLLGWKIPCPPGELPLL 843
            V YLASGCK KEKWRIGTEHEKFGFEFGTL PMKYEQIAEL+N IAERFDWEKVMEGD IIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVA+EMGIGFLG GFQPKWGLKDIPVMPKGRYEIMRNYMPKVGSLGLDMMFRTCTV VNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMR HIWTDTDNNRAGMLPF FDDSFGFEQY DYALD     +  K+ Y  C  +  +  +  K+   PG+ P L
Sbjct:   95 VAYLASGCKSKEKWRIGTEHEKFGFEFGTLRPMKYEQIAELLNGIAERFDWEKVMEGDNIIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGTGFQPKWGLKDIPVMPKGRYEIMRNYMPKVGSLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRSHIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGMSFRDFMNGKLSPIPGDYPTL 372
[Close]
BLAST of EY702594 vs. ExPASy Swiss-Prot
Match: GSH1_MEDTR (Glutamate--cysteine ligase, chloroplastic OS=Medicago truncatula GN=GSH1 PE=2 SV=1)

HSP 1 Score: 460.685 bits (1184), Expect = 4.879e-129
Identity = 224/278 (80.58%), Postives = 243/278 (87.41%), Query Frame = 1 
Query:   13 VGYLASGCKPKEKWRIGTEHEKFGFEFGTLHPMKYEQIAELVNSIAERFDWEKVMEGDYIIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVAKEMGIGFLGIGFQPKWGLKDIPVMPKGRYEIMRNYMPKVGSLGLDMMFRTCTVHVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRGHIWTDTDNNRAGMLPFAFDDSFGFEQYGDYALDGQ*NLLIAKRVY*LCWDV-IQGLLGWKIPCPPGELPLL 843
            + YLASGCK K+KWRIGTEHEKFGFE G+L PMKYEQI+EL+N IAERFDW+KVMEGD IIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVA+EMGIGFLGIGFQPKW  KDIP+MPKGRYEIM+ YMPKVGSLGLDMMFRTCTV VNLDFSSEADMIRKFRAGLALQPIATALFANSPFT+GKPNG++SMR HIWTDTD +R GMLPF FDDSFGFEQY D+ALD     +  K+ Y  C  +  +  L  K+PC PGELP L
Sbjct:   81 IDYLASGCKTKDKWRIGTEHEKFGFELGSLRPMKYEQISELLNGIAERFDWDKVMEGDNIIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWERKDIPMMPKGRYEIMKKYMPKVGSLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTDGKPNGFVSMRSHIWTDTDKDRTGMLPFVFDDSFGFEQYVDFALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLPCIPGELPTL 358
[Close]
BLAST of EY702594 vs. ExPASy Swiss-Prot
Match: GSH1_BRAJU (Glutamate--cysteine ligase, chloroplastic OS=Brassica juncea GN=GSH1 PE=1 SV=1)

HSP 1 Score: 457.218 bits (1175), Expect = 5.394e-128
Identity = 225/276 (81.52%), Postives = 238/276 (86.23%), Query Frame = 1 
Query:   13 VGYLASGCKPKEKWRIGTEHEKFGFEFGTLHPMKYEQIAELVNSIAERFDWEKVMEGDYIIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVAKEMGIGFLGIGFQPKWGLKDIPVMPKGRYEIMRNYMPKVGSLGLDMMFRTCTVHVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRGHIWTDTDNNRAGMLPFAFDDSFGFEQYGDYALDGQ*NLLIAKRVY*LCWDV-IQGLLGWKIPCPPGELP 837
            + YLASGCK KEKWRIGTEHEKFGFE  TL PMKY+QIAEL+NSIAERF+WEKVMEGD IIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVA+EMGIGFLG+GFQPKW  +DIP MPKGRY+IMRNYMPKVGSLGLDMM RTCTV VNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNG+LSMR HIWTDTD +R GMLPF FDDSFGFEQY DYALD           Y  C  +  +  L  K+PC PGELP
Sbjct:   87 IAYLASGCKSKEKWRIGTEHEKFGFEVNTLRPMKYDQIAELLNSIAERFEWEKVMEGDKIIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGMGFQPKWRREDIPTMPKGRYDIMRNYMPKVGSLGLDMMLRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGFLSMRSHIWTDTDKDRTGMLPFVFDDSFGFEQYVDYALDVPMYFAYRNGKYVDCTGMTFRQFLAGKLPCLPGELP 362
[Close]
BLAST of EY702594 vs. ExPASy Swiss-Prot
Match: GSH1_ARATH (Glutamate--cysteine ligase, chloroplastic OS=Arabidopsis thaliana GN=GSH1 PE=1 SV=2)

HSP 1 Score: 454.907 bits (1169), Expect = 2.677e-127
Identity = 223/276 (80.80%), Postives = 239/276 (86.59%), Query Frame = 1 
Query:   13 VGYLASGCKPKEKWRIGTEHEKFGFEFGTLHPMKYEQIAELVNSIAERFDWEKVMEGDYIIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVAKEMGIGFLGIGFQPKWGLKDIPVMPKGRYEIMRNYMPKVGSLGLDMMFRTCTVHVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRGHIWTDTDNNRAGMLPFAFDDSFGFEQYGDYALDGQ*NLLIAKRVY*LCWDV-IQGLLGWKIPCPPGELP 837
            + YLASGCK K+K+RIGTEHEKFGFE  TL PMKY+QIAEL+N IAERF+WEKVMEGD IIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVA+EMGIGFLGIGFQPKW  +DIP+MPKGRY+IMRNYMPKVG+LGLDMM RTCTV VNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNG+LSMR HIWTDTD +R GMLPF FDDSFGFEQY DYALD        K  Y  C  +  +  L  K+PC PGELP
Sbjct:   95 IAYLASGCKTKDKYRIGTEHEKFGFEVNTLRPMKYDQIAELLNGIAERFEWEKVMEGDKIIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWRREDIPIMPKGRYDIMRNYMPKVGTLGLDMMLRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGFLSMRSHIWTDTDKDRTGMLPFVFDDSFGFEQYVDYALDVPMYFAYRKNKYIDCTGMTFRQFLAGKLPCLPGELP 370
[Close]
BLAST of EY702594 vs. ExPASy Swiss-Prot
Match: GSH1B_ORYSI (Glutamate--cysteine ligase B, chloroplastic OS=Oryza sativa subsp. indica GN=GSH1-2 PE=3 SV=2)

HSP 1 Score: 444.506 bits (1142), Expect = 3.618e-124
Identity = 213/278 (76.62%), Postives = 237/278 (85.25%), Query Frame = 1 
Query:   13 VGYLASGCKPKEKWRIGTEHEKFGFEFGTLHPMKYEQIAELVNSIAERFDWEKVMEGDYIIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVAKEMGIGFLGIGFQPKWGLKDIPVMPKGRYEIMRNYMPKVGSLGLDMMFRTCTVHVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRGHIWTDTDNNRAGMLPFAFDDSFGFEQYGDYALDGQ*NLLIAKRVY*LCWDV-IQGLLGWKIPCPPGELPLL 843
            + YL SGCKPKE WRIGTEHEKFGFE  TL P+KY+QI +++N +AERFDW+K++E + +IGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAV +EMGIGFLGIGFQPKW L DIP+MPKGRYEIMRNYMPKVGSLGLDMMFRTCTV VNLDFSSE DMIRKFRAGLALQPIATA+FANSPF EGKPNGYLS+R HIWTDTDNNR+GMLPF FDDSFGFE+Y DYALD     +   + Y  C  +  +  +  K+P  PGELP L
Sbjct:   69 MAYLVSGCKPKENWRIGTEHEKFGFEVDTLRPIKYDQIRDILNGLAERFDWDKIVEENNVIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVGEEMGIGFLGIGFQPKWALSDIPIMPKGRYEIMRNYMPKVGSLGLDMMFRTCTVQVNLDFSSEQDMIRKFRAGLALQPIATAIFANSPFKEGKPNGYLSLRSHIWTDTDNNRSGMLPFVFDDSFGFERYVDYALDVPMYFVYRNKKYIDCTGMSFRDFMVGKLPQAPGELPTL 346
[Close]
BLAST of EY702594 vs. ExPASy Swiss-Prot
Match: GSH1A_ORYSJ (Glutamate--cysteine ligase A, chloroplastic OS=Oryza sativa subsp. japonica GN=GSH1-1 PE=2 SV=1)

HSP 1 Score: 443.736 bits (1140), Expect = 6.172e-124
Identity = 213/278 (76.62%), Postives = 236/278 (84.89%), Query Frame = 1 
Query:   13 VGYLASGCKPKEKWRIGTEHEKFGFEFGTLHPMKYEQIAELVNSIAERFDWEKVMEGDYIIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVAKEMGIGFLGIGFQPKWGLKDIPVMPKGRYEIMRNYMPKVGSLGLDMMFRTCTVHVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRGHIWTDTDNNRAGMLPFAFDDSFGFEQYGDYALDGQ*NLLIAKRVY*LCWDV-IQGLLGWKIPCPPGELPLL 843
            V YL SGCKPKE WRIGTEHEKFGFE  TL P+KY+QI +++N +AERFDW+K++E + +IGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAV +EMGIGFLGIGFQPKW L DIP+MPKGRYEIMRNYMPKVGSLGLDMMFRTCTV VNLDFSSE DMIRKFR GLALQPIATA+FANSPF EGKPNGYLS+R HIWTDTDNNR+GMLPF FDDSFGFE+Y DYALD     +   + Y  C  +  +  +  K+P  PGELP L
Sbjct:   65 VAYLVSGCKPKENWRIGTEHEKFGFEVDTLRPIKYDQIRDILNGLAERFDWDKIVEENNVIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVGEEMGIGFLGIGFQPKWALSDIPIMPKGRYEIMRNYMPKVGSLGLDMMFRTCTVQVNLDFSSEQDMIRKFRTGLALQPIATAIFANSPFKEGKPNGYLSLRSHIWTDTDNNRSGMLPFVFDDSFGFERYVDYALDIPMYFVYRNKKYIDCTGMSFRDFMVGKLPQAPGELPTL 342
[Close]
BLAST of EY702594 vs. ExPASy Swiss-Prot
Match: GSH1B_ORYSJ (Glutamate--cysteine ligase B, chloroplastic OS=Oryza sativa subsp. japonica GN=GSH1-2 PE=3 SV=1)

HSP 1 Score: 442.965 bits (1138), Expect = 1.053e-123
Identity = 212/278 (76.26%), Postives = 236/278 (84.89%), Query Frame = 1 
Query:   13 VGYLASGCKPKEKWRIGTEHEKFGFEFGTLHPMKYEQIAELVNSIAERFDWEKVMEGDYIIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVAKEMGIGFLGIGFQPKWGLKDIPVMPKGRYEIMRNYMPKVGSLGLDMMFRTCTVHVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRGHIWTDTDNNRAGMLPFAFDDSFGFEQYGDYALDGQ*NLLIAKRVY*LCWDV-IQGLLGWKIPCPPGELPLL 843
            + YL SGCKPKE WRIGTEHEKFGFE  TL P+KY+QI +++N +AERFDW+K++E + +IGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAV +EMGIGFLGIGFQPKW L DIP+MPKGRYEIMRNYMPKVGSLGLDMMFRTCTV VNLDFSSE DMIRKFR GLALQPIATA+FANSPF EGKPNGYLS+R HIWTDTDNNR+GMLPF FDDSFGFE+Y DYALD     +   + Y  C  +  +  +  K+P  PGELP L
Sbjct:   69 MAYLVSGCKPKENWRIGTEHEKFGFEVDTLRPIKYDQIRDILNGLAERFDWDKIVEENNVIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVGEEMGIGFLGIGFQPKWALSDIPIMPKGRYEIMRNYMPKVGSLGLDMMFRTCTVQVNLDFSSEQDMIRKFRTGLALQPIATAIFANSPFKEGKPNGYLSLRSHIWTDTDNNRSGMLPFVFDDSFGFERYVDYALDVPMYFVYRNKKYIDCTGMSFRDFMVGKLPQAPGELPTL 346
[Close]
BLAST of EY702594 vs. ExPASy Swiss-Prot
Match: GSH1A_ORYSI (Glutamate--cysteine ligase A, chloroplastic OS=Oryza sativa subsp. indica GN=GSH1-1 PE=2 SV=2)

HSP 1 Score: 442.195 bits (1136), Expect = 1.796e-123
Identity = 212/278 (76.26%), Postives = 235/278 (84.53%), Query Frame = 1 
Query:   13 VGYLASGCKPKEKWRIGTEHEKFGFEFGTLHPMKYEQIAELVNSIAERFDWEKVMEGDYIIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVAKEMGIGFLGIGFQPKWGLKDIPVMPKGRYEIMRNYMPKVGSLGLDMMFRTCTVHVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRGHIWTDTDNNRAGMLPFAFDDSFGFEQYGDYALDGQ*NLLIAKRVY*LCWDV-IQGLLGWKIPCPPGELPLL 843
            V YL SGCKPKE WRIGTEHEKFGFE  TL P+KY+QI +++N +AERFDW+K++E + +IGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAV +EMGIGFLGIGFQPKW L DIP+MPKGRYEIMRNYMPKVGSLGLDMMFRTCTV VNLDFSSE DMIRKF  GLALQPIATA+FANSPF EGKPNGYLS+R HIWTDTDNNR+GMLPF FDDSFGFE+Y DYALD     +   + Y  C  +  +  +  K+P  PGELP L
Sbjct:   65 VAYLVSGCKPKENWRIGTEHEKFGFEVDTLRPIKYDQIRDILNGLAERFDWDKIVEENNVIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHLYQVKAVGEEMGIGFLGIGFQPKWALSDIPIMPKGRYEIMRNYMPKVGSLGLDMMFRTCTVQVNLDFSSEQDMIRKFHTGLALQPIATAIFANSPFKEGKPNGYLSLRSHIWTDTDNNRSGMLPFVFDDSFGFERYVDYALDVPMYFVYRNKKYIDCTGMSFRDFMVGKLPQAPGELPTL 342
The following BLAST results are available for this feature:
BLAST of EY702594 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 9
Match NameE-valueIdentityDescription
GSH1_SOLLC8.592e-13484.17Glutamate--cysteine ligase, chloroplastic OS=Solan... [more]
GSH1_TOBAC1.122e-13384.89Glutamate--cysteine ligase, chloroplastic OS=Nicot... [more]
GSH1_MEDTR4.879e-12980.58Glutamate--cysteine ligase, chloroplastic OS=Medic... [more]
GSH1_BRAJU5.394e-12881.52Glutamate--cysteine ligase, chloroplastic OS=Brass... [more]
GSH1_ARATH2.677e-12780.80Glutamate--cysteine ligase, chloroplastic OS=Arabi... [more]
GSH1B_ORYSI3.618e-12476.62Glutamate--cysteine ligase B, chloroplastic OS=Ory... [more]
GSH1A_ORYSJ6.172e-12476.62Glutamate--cysteine ligase A, chloroplastic OS=Ory... [more]
GSH1B_ORYSJ1.053e-12376.26Glutamate--cysteine ligase B, chloroplastic OS=Ory... [more]
GSH1A_ORYSI1.796e-12376.26Glutamate--cysteine ligase A, chloroplastic OS=Ory... [more]
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Properties
Property NameValue
Genbank descriptionCS00-C3-701-009-H03-CT.F Sweet orange fruit, development stadium (2 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY702594 ID=EY702594; Name=EY702594; organism=Citrus sinensis; type=EST; length=868bp
CCACGCGTCCGAGTTGGATACCTTGCCTCTGGGTGCAAGCCTAAGGAAAA
GTGGAGAATTGGTACAGAACATGAGAAGTTTGGTTTTGAGTTTGGAACTT
TACATCCTATGAAATACGAACAAATAGCCGAGTTGGTTAACAGCATTGCT
GAGAGGTTCGATTGGGAGAAAGTAATGGAAGGAGATTACATCATAGGACT
GAAACAAGGGAAACAAAGCATATCACTTGAACCTGGCGGTCAGTTTGAGC
TTAGTGGTGCACCCCTTGAAACTCTGCATCAAACTTGTGCTGAGGTTAAT
TCACACCTATATCAGGTGAAAGCTGTTGCAAAAGAAATGGGAATTGGATT
TTTAGGAATTGGCTTCCAGCCTAAGTGGGGACTTAAAGATATACCTGTTA
TGCCAAAGGGAAGATATGAGATTATGCGGAACTACATGCCTAAAGTTGGA
TCACTCGGACTTGATATGATGTTTAGGACATGCACTGTTCACGTTAATTT
GGACTTTAGTTCGGAAGCCGACATGATCCGCAAATTTCGTGCTGGTCTTG
CTTTGCAGCCAATAGCAACAGCTCTATTTGCAAATTCACCATTTACAGAA
GGAAAACCAAATGGCTATCTCAGCATGAGAGGCCACATCTGGACAGATAC
CGATAACAATCGTGCTGGCATGCTTCCCTTTGCTTTTGATGACTCCTTTG
GGTTTGAGCAATATGGTGATTACGCCCTTGATGGTCAATGAAATTTGTTA
ATCGCAAAAAGAGTATATTGATTGTGCTGGGATGTCATCCAGGGACTCTT
GGGCTGGAAAATTCCCTGCCCTCCTGGGGAATTGCCACTCCTAATGATTG
GGAAAATCCCCTGACACC
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