EY702587 | Citrus Genome Database

Overview


Name	EY702587
Unique Name	EY702587
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	876

Libraries


Library Name	Type
Sweet orange fruit, development stadium (2 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
EY702587_ssr858	microsatellite	EY702587_ssr858:858..869.	BLAST: Citrus ESTs to Prunus persica proteins V1

Homology

[Close]

BLAST of EY702587 vs. ExPASy Swiss-Prot
Match: RL142_ARATH (60S ribosomal protein L14-2 OS=Arabidopsis thaliana GN=RPL14B PE=2 SV=1)

HSP 1 Score: 138.658 bits (348), Expect = 3.109e-44
Identity = 69/84 (82.14%), Postives = 74/84 (88.10%), Query Frame = 2

Query:  131 LDQNRALVDAPDMVRSQMNFKRVSLTDIKIDINRVPRKKSLIEAMEKADVNNKWENSSWGRKLIVQKRRAALNDFDTFKPHVGK 382
            +DQNRALVDAPDM R QMNFKR+SLTDI IDINRVP+KK+LIEAMEKADV NKWE SSWGRKLIVQKRRA LNDFD FK  + K
Sbjct:   31 VDQNRALVDAPDMERIQMNFKRLSLTDIVIDINRVPKKKALIEAMEKADVKNKWEKSSWGRKLIVQKRRANLNDFDRFKIMLAK 114

HSP 2 Score: 44.2838 bits (103), Expect = 3.109e-44
Identity = 21/25 (84.00%), Postives = 23/25 (92.00%), Query Frame = 1

Query:   40 MPFKRYVEI*RVALVNYGKDYGKLV 114
            M FKRYVEI RVALVNYG+D+GKLV
Sbjct:    1 MGFKRYVEIGRVALVNYGEDHGKLV 25

HSP 3 Score: 38.5058 bits (88), Expect = 3.109e-44
Identity = 19/28 (67.86%), Postives = 22/28 (78.57%), Query Frame = 3

Query:  360 RSSLMLANIKKGGLVRQELAKLRKENAA 443
            R  +MLA IKK G+VRQELAKL+KE  A
Sbjct:  107 RFKIMLAKIKKAGVVRQELAKLKKEITA 134

[Close]

BLAST of EY702587 vs. ExPASy Swiss-Prot
Match: RL141_ARATH (60S ribosomal protein L14-1 OS=Arabidopsis thaliana GN=RPL14A PE=2 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 6.839e-44
Identity = 69/84 (82.14%), Postives = 73/84 (86.90%), Query Frame = 2

Query:  131 LDQNRALVDAPDMVRSQMNFKRVSLTDIKIDINRVPRKKSLIEAMEKADVNNKWENSSWGRKLIVQKRRAALNDFDTFKPHVGK 382
            +DQNRALVDAPDM R QMN KR+SLTDI IDINRVP+KK LIEAMEKADV NKWE SSWGRKLIVQKRRAALNDFD FK  + K
Sbjct:   31 VDQNRALVDAPDMERIQMNLKRLSLTDIVIDINRVPKKKVLIEAMEKADVKNKWEKSSWGRKLIVQKRRAALNDFDRFKIMLAK 114

HSP 2 Score: 44.669 bits (104), Expect = 6.839e-44
Identity = 21/25 (84.00%), Postives = 23/25 (92.00%), Query Frame = 1

Query:   40 MPFKRYVEI*RVALVNYGKDYGKLV 114
            M FKR+VEI RVALVNYG+DYGKLV
Sbjct:    1 MGFKRFVEIGRVALVNYGEDYGKLV 25

HSP 3 Score: 38.1206 bits (87), Expect = 6.839e-44
Identity = 18/28 (64.29%), Postives = 23/28 (82.14%), Query Frame = 3

Query:  360 RSSLMLANIKKGGLVRQELAKLRKENAA 443
            R  +MLA IK+ G+VRQELAKL++E AA
Sbjct:  107 RFKIMLAKIKRAGIVRQELAKLKREIAA 134

[Close]

BLAST of EY702587 vs. ExPASy Swiss-Prot
Match: RL14_PEA (Probable 60S ribosomal protein L14 OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 2.499e-39
Identity = 61/84 (72.62%), Postives = 70/84 (83.33%), Query Frame = 2

Query:  131 LDQNRALVDAPDMVRSQMNFKRVSLTDIKIDINRVPRKKSLIEAMEKADVNNKWENSSWGRKLIVQKRRAALNDFDTFKPHVGK 382
            +DQ RALVDAPDM RS +NFKR+SLTD+KIDI RVP+KK LI+A+E ADV NKW  SSWGRKLIV+K RAALNDFD FK  + K
Sbjct:   31 IDQTRALVDAPDMERSPINFKRLSLTDLKIDIKRVPKKKDLIKALEAADVKNKWAKSSWGRKLIVKKTRAALNDFDRFKIMLAK 114

HSP 2 Score: 48.1358 bits (113), Expect = 2.499e-39
Identity = 21/25 (84.00%), Postives = 24/25 (96.00%), Query Frame = 1

Query:   40 MPFKRYVEI*RVALVNYGKDYGKLV 114
            MPFKR+VEI RVAL+NYGKDYG+LV
Sbjct:    1 MPFKRFVEIGRVALINYGKDYGRLV 25

HSP 3 Score: 31.187 bits (69), Expect = 2.499e-39
Identity = 16/27 (59.26%), Postives = 19/27 (70.37%), Query Frame = 3

Query:  360 RSSLMLANIKKGGLVRQELAKLRKENA 440
            R  +MLA IK+   VRQELAKL+K  A
Sbjct:  107 RFKIMLAKIKRAAGVRQELAKLKKTAA 133

[Close]

BLAST of EY702587 vs. ExPASy Swiss-Prot
Match: RL14_MOUSE (60S ribosomal protein L14 OS=Mus musculus GN=Rpl14 PE=2 SV=3)

HSP 1 Score: 70.0922 bits (170), Expect = 6.974e-15
Identity = 39/101 (38.61%), Postives = 54/101 (53.47%), Query Frame = 2

Query:  131 LDQNRALVDAP--DMVRSQMNFKRVSLTDIKIDINRVPRKKSLIEAMEKADVNNKWENSSWGRKLIVQKRRAALNDFDTFKPHVGKHQEGWTCSTGACKTQ 427
            +DQNRALVD P   + R  M FK + LTD  +      R+K + +A EKAD+N KW  + W +K+  ++R+A + DFD FK    K        T   K Q
Sbjct:   31 IDQNRALVDGPCTRVRRQAMPFKCMQLTDFILKFPHSARQKYVRKAWEKADINTKWAATRWAKKIDARERKAKMTDFDRFKVMKAKKMRNRIIKTEVKKLQ 131

HSP 2 Score: 31.9574 bits (71), Expect = 6.974e-15
Identity = 14/25 (56.00%), Postives = 19/25 (76.00%), Query Frame = 1

Query:   40 MPFKRYVEI*RVALVNYGKDYGKLV 114
            M F+RYVE+ RVA +++G   GKLV
Sbjct:    1 MVFRRYVEVGRVAYISFGPHAGKLV 25

[Close]

BLAST of EY702587 vs. ExPASy Swiss-Prot
Match: RL14_RAT (60S ribosomal protein L14 OS=Rattus norvegicus GN=Rpl14 PE=1 SV=3)

HSP 1 Score: 70.0922 bits (170), Expect = 1.990e-14
Identity = 39/101 (38.61%), Postives = 54/101 (53.47%), Query Frame = 2

Query:  131 LDQNRALVDAP--DMVRSQMNFKRVSLTDIKIDINRVPRKKSLIEAMEKADVNNKWENSSWGRKLIVQKRRAALNDFDTFKPHVGKHQEGWTCSTGACKTQ 427
            +DQNRALVD P   + R  M FK + LTD  +      R+K + +A EKAD+N KW  + W +K+  ++R+A + DFD FK    K        T   K Q
Sbjct:   31 IDQNRALVDGPCTRVRRQAMPFKCMQLTDFILKFPHSARQKYVRKAWEKADINTKWAATRWAKKIDARERKAKMTDFDRFKVMKAKKMRNRIIKTEVKKLQ 131

HSP 2 Score: 30.4166 bits (67), Expect = 1.990e-14
Identity = 13/25 (52.00%), Postives = 19/25 (76.00%), Query Frame = 1

Query:   40 MPFKRYVEI*RVALVNYGKDYGKLV 114
            M F+R+VE+ RVA +++G   GKLV
Sbjct:    1 MVFRRFVEVGRVAYISFGPHAGKLV 25

[Close]

BLAST of EY702587 vs. ExPASy Swiss-Prot
Match: RL14_HUMAN (60S ribosomal protein L14 OS=Homo sapiens GN=RPL14 PE=1 SV=4)

HSP 1 Score: 67.3958 bits (163), Expect = 9.572e-14
Identity = 33/81 (40.74%), Postives = 49/81 (60.49%), Query Frame = 2

Query:  131 LDQNRALVDAP--DMVRSQMNFKRVSLTDIKIDINRVPRKKSLIEAMEKADVNNKWENSSWGRKLIVQKRRAALNDFDTFK 367
            +DQNRALVD P   + R  M FK + LTD  +       +K + +A +KAD+N KW  + W +K+  ++R+A + DFD FK
Sbjct:   31 IDQNRALVDGPCTQVRRQAMPFKCMQLTDFILKFPHSAHQKYVRQAWQKADINTKWAATRWAKKIEARERKAKMTDFDRFK 111

HSP 2 Score: 30.8018 bits (68), Expect = 9.572e-14
Identity = 14/25 (56.00%), Postives = 19/25 (76.00%), Query Frame = 1

Query:   40 MPFKRYVEI*RVALVNYGKDYGKLV 114
            M F+R+VE+ RVA V++G   GKLV
Sbjct:    1 MVFRRFVEVGRVAYVSFGPHAGKLV 25

[Close]

BLAST of EY702587 vs. ExPASy Swiss-Prot
Match: RL14_PIG (60S ribosomal protein L14 OS=Sus scrofa GN=RPL14 PE=2 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 1.244e-13
Identity = 33/81 (40.74%), Postives = 50/81 (61.73%), Query Frame = 2

Query:  131 LDQNRALVDAP--DMVRSQMNFKRVSLTDIKIDINRVPRKKSLIEAMEKADVNNKWENSSWGRKLIVQKRRAALNDFDTFK 367
            +DQNRALVD P   + R  M FK + LTD  +      R+K + +A EKAD+N KW  + W +K+  ++++A + DFD +K
Sbjct:   31 IDQNRALVDGPCTQVRRQAMPFKCMQLTDFILKFPHSARQKYVRKAWEKADINAKWAATRWAKKIEAREKKAKMTDFDRYK 111

HSP 2 Score: 30.8018 bits (68), Expect = 1.244e-13
Identity = 14/25 (56.00%), Postives = 19/25 (76.00%), Query Frame = 1

Query:   40 MPFKRYVEI*RVALVNYGKDYGKLV 114
            M F+R+VE+ RVA V++G   GKLV
Sbjct:    1 MVFRRFVEVGRVAYVSFGPHAGKLV 25

[Close]

BLAST of EY702587 vs. ExPASy Swiss-Prot
Match: RL14_BOVIN (60S ribosomal protein L14 OS=Bos taurus GN=RPL14 PE=2 SV=3)

HSP 1 Score: 67.0106 bits (162), Expect = 1.244e-13
Identity = 33/81 (40.74%), Postives = 50/81 (61.73%), Query Frame = 2

Query:  131 LDQNRALVDAP--DMVRSQMNFKRVSLTDIKIDINRVPRKKSLIEAMEKADVNNKWENSSWGRKLIVQKRRAALNDFDTFK 367
            +DQNRALVD P   + R  M FK + LTD  +      R+K + +A EKAD+N KW  + W +K+  ++++A + DFD +K
Sbjct:   31 IDQNRALVDGPCTQVRRQAMPFKCMQLTDFILKFPHSARQKYVRKAWEKADINAKWAATRWAKKIEAREKKAKMTDFDRYK 111

HSP 2 Score: 30.8018 bits (68), Expect = 1.244e-13
Identity = 14/25 (56.00%), Postives = 19/25 (76.00%), Query Frame = 1

Query:   40 MPFKRYVEI*RVALVNYGKDYGKLV 114
            M F+R+VE+ RVA V++G   GKLV
Sbjct:    1 MVFRRFVEVGRVAYVSFGPHAGKLV 25

[Close]

BLAST of EY702587 vs. ExPASy Swiss-Prot
Match: RL14_LUMRU (60S ribosomal protein L14 OS=Lumbricus rubellus GN=RPL14 PE=3 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 3.618e-13
Identity = 31/81 (38.27%), Postives = 48/81 (59.26%), Query Frame = 2

Query:  131 LDQNRALVDAP--DMVRSQMNFKRVSLTDIKIDINRVPRKKSLIEAMEKADVNNKWENSSWGRKLIVQKRRAALNDFDTFK 367
            +DQNRALVD P   + R  MNFK + LT++K       +   + +A EK +++ KWE S   +K+  +++R  L DF+ FK
Sbjct:   31 IDQNRALVDGPCTHVARKSMNFKELELTNLKAKFPHSAKTGVVKKAWEKDEISKKWEESHLAKKIAAKEKRKTLTDFERFK 111

HSP 2 Score: 32.7278 bits (73), Expect = 3.618e-13
Identity = 16/25 (64.00%), Postives = 18/25 (72.00%), Query Frame = 1

Query:   40 MPFKRYVEI*RVALVNYGKDYGKLV 114
            M F+R+VEI RVA   YG D GKLV
Sbjct:    1 MSFRRFVEIGRVARAVYGPDQGKLV 25

[Close]

BLAST of EY702587 vs. ExPASy Swiss-Prot
Match: RL14_GRIJA (60S ribosomal protein L14 OS=Griffithsia japonica GN=RPL14 PE=2 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 8.486e-12
Identity = 37/89 (41.57%), Postives = 53/89 (59.55%), Query Frame = 2

Query:  131 LDQNRALVDAPD--MVRSQMNFKRVSLTDIKIDINRVPRKKSLIEAMEK---ADVNNKWENSSWGRKLIVQKRRAALNDFDTFKPHVGK 382
            LDQNR LVD+P   + R  +N KR++LT IK+D   + R   + E   K   A V+  +  S WG+KL  +++RAAL+DF  FK  V +
Sbjct:   31 LDQNRILVDSPSHGLKRKVINVKRIALTSIKVD--DIARGAPVAEVKSKYTAAKVDETFAASGWGKKLAKREKRAALDDFGRFKVMVAR 117

HSP 2 Score: 30.8018 bits (68), Expect = 8.486e-12
Identity = 15/25 (60.00%), Postives = 18/25 (72.00%), Query Frame = 1

Query:   40 MPFKRYVEI*RVALVNYGKDYGKLV 114
            M F R+VE  RVALV+YG+   KLV
Sbjct:    1 MTFTRFVEAGRVALVSYGEHLNKLV 25

The following BLAST results are available for this feature:

BLAST of EY702587 vs. ExPASy Swiss-Prot

BLAST of EY702587 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 10


Match Name	E-value	Identity	Description
RL142_ARATH	3.109e-44	82.14	60S ribosomal protein L14-2 OS=Arabidopsis thalian...	[more]
RL141_ARATH	6.839e-44	82.14	60S ribosomal protein L14-1 OS=Arabidopsis thalian...	[more]
RL14_PEA	2.499e-39	72.62	Probable 60S ribosomal protein L14 OS=Pisum sativu...	[more]
RL14_MOUSE	6.974e-15	38.61	60S ribosomal protein L14 OS=Mus musculus GN=Rpl14...	[more]
RL14_RAT	1.990e-14	38.61	60S ribosomal protein L14 OS=Rattus norvegicus GN=...	[more]
RL14_HUMAN	9.572e-14	40.74	60S ribosomal protein L14 OS=Homo sapiens GN=RPL14...	[more]
RL14_PIG	1.244e-13	40.74	60S ribosomal protein L14 OS=Sus scrofa GN=RPL14 P...	[more]
RL14_BOVIN	1.244e-13	40.74	60S ribosomal protein L14 OS=Bos taurus GN=RPL14 P...	[more]
RL14_LUMRU	3.618e-13	38.27	60S ribosomal protein L14 OS=Lumbricus rubellus GN...	[more]
RL14_GRIJA	8.486e-12	41.57	60S ribosomal protein L14 OS=Griffithsia japonica ...	[more]

Properties


Property Name	Value
Genbank description	CS00-C3-701-009-G07-CT.F Sweet orange fruit, development stadium (2 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY702587 ID=EY702587; Name=EY702587; organism=Citrus sinensis; type=EST; length=876bp
CTCCTCGCTCCTCCGGGAGAGCCTTGAATATCTTCGAAAATGCCGTTCAA
GAGGTACGTGGAGATATGAAGAGTTGCTCTCGTTAATTACGGCAAGGACT
ATGGGAAGCTTGTGGGCGGGCGGCGGTGGCCTTGATCAAAACAGGGCTCT
TGTTGATGCCCCTGATATGGTGAGGAGCCAAATGAACTTCAAGAGGGTTT
CTCTCACGGATATTAAGATTGACATCAATCGAGTTCCCAGGAAGAAGTCT
TTGATTGAAGCCATGGAGAAGGCTGATGTGAATAACAAATGGGAAAACAG
CTCCTGGGGCAGGAAGCTGATTGTCCAGAAGAGGAGAGCGGCACTGAATG
ATTTTGATACGTTCAAGCCTCATGTTGGCAAACATCAAGAAGGGTGGACT
TGTTCGACAGGAGCTTGCAAAACTCAGGAAGGAGAATGCAGCATAGAATT
TCTCTCTTGGGGTCCCCCTTTCTGGGTTCTAGCTCTTACCGCTTCAATTT
GATTCTATTAATGGGTTGATTAATATGACAAAACATCCGCTTGGGCGTTT
TGGGTTAATTGTTATTCTGGACAGAATTTCTAGATACGCCCCGGCGCGCC
CGCGGGCCGGGGGGCCCAGGCGCGCCCGCCGCGGCCGGCGCCCCCCCCCC
GCGCCGCCCGTGCAGGCGCGGCGGTCGCTCGCCGCGCCTCGCGCGTCCCC
CCCCCTCCCGCCCGTCCCATCCGCCCCCGCCACGCCCCGCCCCACCGCGC
CCGGCCGGGTGGTCAGCGGGGTGCCATCGTCTGGCCCCCCGGCCCCCCGC
CCCGCACCCCCGCTGGGGGCCTGCCCCGTGATGCTGTGCCGGGGGCCGGT
GTGGTGGCCGCCCGCCCGCCCCCCCC