CX070097

Overview
NameCX070097
Unique NameCX070097
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length848
Libraries
Library NameType
Parent Washington Navel Orange Callus cDNA Library UCRCS08-2cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CX070097 vs. ExPASy Swiss-Prot
Match: TBG1_ARATH (Tubulin gamma-1 chain OS=Arabidopsis thaliana GN=TUBG1 PE=2 SV=1)

HSP 1 Score: 454.136 bits (1167), Expect = 4.382e-127
Identity = 232/237 (97.89%), Postives = 234/237 (98.73%), Query Frame = 3
Query:  138 QMETSDVVVQPYNSLLTLKRLTLNADCVVVLDNTALNRIAVERLHLQNPTFAQTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHFLMTGYTPLTVERQANVIRKTTVLDVMRRLLQTKNIMVSSYARTKEASQAKYISILNIIQGEVDPTQVHESLQRIRERKLVNFIEWGPASIQVALSRKSPYVQTAHRVSGLMLASHTSIRHLFSKCLSQYEKLRKKQAFLDNYR 848
            QMETSDVVVQPYNSLLTLKRLTLNADCVVVLDNTAL RIAVERLHL NPTFAQTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHFLMTGYTPLTVERQANVIRKTTVLDVMRRLLQTKNIMVSSYAR KEASQAKYISILNIIQGEVDPTQVHESLQRIRERKLVNFIEWGPASIQVALS+KSPYVQTAHRVSGLMLASHTSIRHLFSKCLSQY+KLRKKQAFLDNYR
Sbjct:  175 QMETSDVVVQPYNSLLTLKRLTLNADCVVVLDNTALGRIAVERLHLTNPTFAQTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHFLMTGYTPLTVERQANVIRKTTVLDVMRRLLQTKNIMVSSYARNKEASQAKYISILNIIQGEVDPTQVHESLQRIRERKLVNFIEWGPASIQVALSKKSPYVQTAHRVSGLMLASHTSIRHLFSKCLSQYDKLRKKQAFLDNYR 411          
BLAST of CX070097 vs. ExPASy Swiss-Prot
Match: TBG2_ARATH (Tubulin gamma-2 chain OS=Arabidopsis thaliana GN=TUBG2 PE=2 SV=1)

HSP 1 Score: 452.981 bits (1164), Expect = 9.762e-127
Identity = 230/237 (97.05%), Postives = 235/237 (99.16%), Query Frame = 3
Query:  138 QMETSDVVVQPYNSLLTLKRLTLNADCVVVLDNTALNRIAVERLHLQNPTFAQTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHFLMTGYTPLTVERQANVIRKTTVLDVMRRLLQTKNIMVSSYARTKEASQAKYISILNIIQGEVDPTQVHESLQRIRERKLVNFIEWGPASIQVALSRKSPYVQTAHRVSGLMLASHTSIRHLFSKCLSQYEKLRKKQAFLDNYR 848
            QMETSDVVVQPYNSLLTLKRLTLNADCVVVLDNTALNRIAVERLHL NPTFAQTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHFLMTGYTPLTVERQANVIRKTTVLDVMRRLLQTKNIMVSSYAR KEASQAKYISILNIIQGEVDPTQVHESLQRIRERKLVNFI+WGPASIQVALS+KSPYVQT+HRVSGLMLASHTSIRHLFS+CLSQY+KLRKKQAFLDNYR
Sbjct:  175 QMETSDVVVQPYNSLLTLKRLTLNADCVVVLDNTALNRIAVERLHLTNPTFAQTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHFLMTGYTPLTVERQANVIRKTTVLDVMRRLLQTKNIMVSSYARNKEASQAKYISILNIIQGEVDPTQVHESLQRIRERKLVNFIDWGPASIQVALSKKSPYVQTSHRVSGLMLASHTSIRHLFSRCLSQYDKLRKKQAFLDNYR 411          
BLAST of CX070097 vs. ExPASy Swiss-Prot
Match: TBG2_ORYSJ (Tubulin gamma-2 chain OS=Oryza sativa subsp. japonica GN=TUBG2 PE=2 SV=1)

HSP 1 Score: 451.825 bits (1161), Expect = 2.175e-126
Identity = 230/237 (97.05%), Postives = 232/237 (97.89%), Query Frame = 3
Query:  138 QMETSDVVVQPYNSLLTLKRLTLNADCVVVLDNTALNRIAVERLHLQNPTFAQTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHFLMTGYTPLTVERQANVIRKTTVLDVMRRLLQTKNIMVSSYARTKEASQAKYISILNIIQGEVDPTQVHESLQRIRERKLVNFIEWGPASIQVALSRKSPYVQTAHRVSGLMLASHTSIRHLFSKCLSQYEKLRKKQAFLDNYR 848
            QMETSDVVVQPYNSLLTLKRLTLNADCVVVLDNTALNRIAVERLHL NPTFAQTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHFLMTGYTPLTVERQ N+IRKTTVLDVMRRLLQTKNIMVSSYAR KEASQAKYISILNIIQGEVDPTQVHESLQRIRERKLVNFIEWGPASIQVALSRKSPYVQT HRVSGLMLA+HTSIRHLFSKCL QYEKLRKKQAFLDNYR
Sbjct:  175 QMETSDVVVQPYNSLLTLKRLTLNADCVVVLDNTALNRIAVERLHLANPTFAQTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHFLMTGYTPLTVERQVNMIRKTTVLDVMRRLLQTKNIMVSSYARNKEASQAKYISILNIIQGEVDPTQVHESLQRIRERKLVNFIEWGPASIQVALSRKSPYVQTTHRVSGLMLANHTSIRHLFSKCLGQYEKLRKKQAFLDNYR 411          
BLAST of CX070097 vs. ExPASy Swiss-Prot
Match: TBG_PHYPA (Tubulin gamma chain OS=Physcomitrella patens GN=TUBG1 PE=3 SV=1)

HSP 1 Score: 450.284 bits (1157), Expect = 6.327e-126
Identity = 228/237 (96.20%), Postives = 233/237 (98.31%), Query Frame = 3
Query:  138 QMETSDVVVQPYNSLLTLKRLTLNADCVVVLDNTALNRIAVERLHLQNPTFAQTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHFLMTGYTPLTVERQANVIRKTTVLDVMRRLLQTKNIMVSSYARTKEASQAKYISILNIIQGEVDPTQVHESLQRIRERKLVNFIEWGPASIQVALSRKSPYVQTAHRVSGLMLASHTSIRHLFSKCLSQYEKLRKKQAFLDNYR 848
            QMETSDVVVQPYNSLLTLKRLTLNADCVVVLDNTALNRIAV+RLH+ NPTFAQTNSLVSTVMSASTTTLRYPGYMNNDLVGL+ASLIPTPRCHFLMTGYTPLTVERQAN IRKTTVLDVMRRLLQ KNIMVSSYARTKEASQAKYISILNIIQGEVDPTQVH+SLQRIRERKL NFIEWGPASIQVALSRKSPYVQTAHRVSGLMLASHTSIRHLFSKC+SQYEKLRKKQAFLDNYR
Sbjct:  175 QMETSDVVVQPYNSLLTLKRLTLNADCVVVLDNTALNRIAVDRLHIPNPTFAQTNSLVSTVMSASTTTLRYPGYMNNDLVGLVASLIPTPRCHFLMTGYTPLTVERQANAIRKTTVLDVMRRLLQAKNIMVSSYARTKEASQAKYISILNIIQGEVDPTQVHKSLQRIRERKLANFIEWGPASIQVALSRKSPYVQTAHRVSGLMLASHTSIRHLFSKCISQYEKLRKKQAFLDNYR 411          
BLAST of CX070097 vs. ExPASy Swiss-Prot
Match: TBG3_MAIZE (Tubulin gamma-3 chain (Fragment) OS=Zea mays GN=TUBG3 PE=2 SV=1)

HSP 1 Score: 449.129 bits (1154), Expect = 1.410e-125
Identity = 228/237 (96.20%), Postives = 232/237 (97.89%), Query Frame = 3
Query:  138 QMETSDVVVQPYNSLLTLKRLTLNADCVVVLDNTALNRIAVERLHLQNPTFAQTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHFLMTGYTPLTVERQANVIRKTTVLDVMRRLLQTKNIMVSSYARTKEASQAKYISILNIIQGEVDPTQVHESLQRIRERKLVNFIEWGPASIQVALSRKSPYVQTAHRVSGLMLASHTSIRHLFSKCLSQYEKLRKKQAFLDNYR 848
            Q+ETSDVVVQPYNSLLTLKRLTLNADCVVVLDNTALNRIAVERLHL NPTFAQTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHFLMTGYTPLTVERQ N+IRKTTVLDVMRRLLQTKNIMVSSYARTKEASQAKYISILNIIQGEVDPTQVHESLQRIRERKLVNFI+W PASIQVALSRKSPYVQT HRVSGLMLA+HTSIRHLFSKCL QYEKLRKKQAFLDNYR
Sbjct:  127 QVETSDVVVQPYNSLLTLKRLTLNADCVVVLDNTALNRIAVERLHLSNPTFAQTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHFLMTGYTPLTVERQVNMIRKTTVLDVMRRLLQTKNIMVSSYARTKEASQAKYISILNIIQGEVDPTQVHESLQRIRERKLVNFIDWAPASIQVALSRKSPYVQTTHRVSGLMLANHTSIRHLFSKCLGQYEKLRKKQAFLDNYR 363          
BLAST of CX070097 vs. ExPASy Swiss-Prot
Match: TBG2_MAIZE (Tubulin gamma-2 chain OS=Zea mays GN=TUBG2 PE=2 SV=1)

HSP 1 Score: 448.743 bits (1153), Expect = 1.841e-125
Identity = 227/237 (95.78%), Postives = 231/237 (97.47%), Query Frame = 3
Query:  138 QMETSDVVVQPYNSLLTLKRLTLNADCVVVLDNTALNRIAVERLHLQNPTFAQTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHFLMTGYTPLTVERQANVIRKTTVLDVMRRLLQTKNIMVSSYARTKEASQAKYISILNIIQGEVDPTQVHESLQRIRERKLVNFIEWGPASIQVALSRKSPYVQTAHRVSGLMLASHTSIRHLFSKCLSQYEKLRKKQAFLDNYR 848
            QMETSDVVVQPYNSLLTLKRLTLNADCVVVLDNTALNRIAVERLHL NPTFAQTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHFLMTGYTPLTVERQ N+IRKTTVLDVMRRLLQTKN+MVSSYARTKEASQAKYISILNIIQGEVDPTQVHESLQRIRERKLVNFI+W PASIQVALSRKSPYVQT HRVSGLMLA+HTSIRHLF KCL QYEKLRKKQAFLDNYR
Sbjct:  175 QMETSDVVVQPYNSLLTLKRLTLNADCVVVLDNTALNRIAVERLHLSNPTFAQTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHFLMTGYTPLTVERQVNMIRKTTVLDVMRRLLQTKNVMVSSYARTKEASQAKYISILNIIQGEVDPTQVHESLQRIRERKLVNFIDWAPASIQVALSRKSPYVQTTHRVSGLMLANHTSIRHLFGKCLGQYEKLRKKQAFLDNYR 411          
BLAST of CX070097 vs. ExPASy Swiss-Prot
Match: TBG1_MAIZE (Tubulin gamma-1 chain OS=Zea mays GN=TUBG1 PE=2 SV=1)

HSP 1 Score: 447.588 bits (1150), Expect = 4.101e-125
Identity = 228/237 (96.20%), Postives = 231/237 (97.47%), Query Frame = 3
Query:  138 QMETSDVVVQPYNSLLTLKRLTLNADCVVVLDNTALNRIAVERLHLQNPTFAQTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHFLMTGYTPLTVERQANVIRKTTVLDVMRRLLQTKNIMVSSYARTKEASQAKYISILNIIQGEVDPTQVHESLQRIRERKLVNFIEWGPASIQVALSRKSPYVQTAHRVSGLMLASHTSIRHLFSKCLSQYEKLRKKQAFLDNYR 848
            Q+ETSDVVVQPYNSLLTLKRLTLNADCVVVLDNTALNRIAVERLHL NPTFAQTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHFLMTGYTPLTVERQ N+IRKTTVLDVMRRLLQTKNIMVSSYARTKEASQAKYISILNIIQGEVDPTQVHESLQRIRERKLVNFI W PASIQVALSRKSPYVQT HRVSGLMLA+HTSIRHLFSKCL QYEKLRKKQAFLDNYR
Sbjct:  175 QVETSDVVVQPYNSLLTLKRLTLNADCVVVLDNTALNRIAVERLHLSNPTFAQTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHFLMTGYTPLTVERQVNMIRKTTVLDVMRRLLQTKNIMVSSYARTKEASQAKYISILNIIQGEVDPTQVHESLQRIRERKLVNFIYWAPASIQVALSRKSPYVQTTHRVSGLMLANHTSIRHLFSKCLGQYEKLRKKQAFLDNYR 411          
BLAST of CX070097 vs. ExPASy Swiss-Prot
Match: TBG_ANEPH (Tubulin gamma chain OS=Anemia phyllitidis GN=TUBG PE=2 SV=1)

HSP 1 Score: 443.736 bits (1140), Expect = 5.922e-124
Identity = 226/238 (94.96%), Postives = 233/238 (97.90%), Query Frame = 3
Query:  138 QMETSDVVVQPYNSLLTL-KRLTLNADCVVVLDNTALNRIAVERLHLQNPTFAQTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHFLMTGYTPLTVERQANVIRKTTVLDVMRRLLQTKNIMVSSYARTKEASQAKYISILNIIQGEVDPTQVHESLQRIRERKLVNFIEWGPASIQVALSRKSPYVQTAHRVSGLMLASHTSIRHLFSKCLSQYEKLRKKQAFLDNYR 848
            QMETSDVVVQPYNSLLTL KRLT+NADCVVVLDNTALNRIAV+RLH+ NPTFAQTNSLVSTVMSASTTTLRYPGYMNNDLVGL+ASLIPTPRCHFLMTGYTPLTVERQAN IRKTTVLDVMRRLLQ KNIMVSSYARTKEASQAKYISILNIIQGEVDPTQVH+SLQRIRERKL NFIEWGPASIQVALSRKSPYVQTAHRVSGLMLASHTSIRHLFSKC++QYEKLRKKQAFLDNYR
Sbjct:  175 QMETSDVVVQPYNSLLTLNKRLTINADCVVVLDNTALNRIAVDRLHIPNPTFAQTNSLVSTVMSASTTTLRYPGYMNNDLVGLVASLIPTPRCHFLMTGYTPLTVERQANAIRKTTVLDVMRRLLQAKNIMVSSYARTKEASQAKYISILNIIQGEVDPTQVHKSLQRIRERKLANFIEWGPASIQVALSRKSPYVQTAHRVSGLMLASHTSIRHLFSKCINQYEKLRKKQAFLDNYR 412          
BLAST of CX070097 vs. ExPASy Swiss-Prot
Match: TBG_CHLRE (Tubulin gamma chain OS=Chlamydomonas reinhardtii GN=TUBG PE=3 SV=1)

HSP 1 Score: 365.54 bits (937), Expect = 2.050e-100
Identity = 188/245 (76.73%), Postives = 213/245 (86.94%), Query Frame = 3
Query:  138 QMETSDVVVQPYNSLLTLKRLTLNADCVVVLDNTALNRIAVERLHLQNPTFAQTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHFLMTGYTPLTVERQANV----IRKTTVLDVMRRLLQTKNIMVSSYARTKEASQAKYISILNIIQGEVDPTQVHESLQRIRERKLVNFIEWGPASIQVALSRKSPYVQTAHRVSGLMLASHTSIRHLFSKCLSQYEKL----RKKQAFLDNYR 848
            Q E+SDVVVQPYNSLLTLKRLTL+AD VVVLDNTAL++IAVERLHL  P   Q NSL++TVM+ASTTTLRYPGYMNNDLVGL+ASLIPTPRCHFLMTGYTPLT E  A      IRKTTVLDVMRRLLQ KNIMVS++ ++++ + AKYISILNIIQGEVDP+QVH+SLQRIRERK  NFIEWGPASIQVALS+KSPYVQTAHRVSGLMLA+HTS+RHLF+K L  YEKL    +++QAF+  YR
Sbjct:  175 QSESSDVVVQPYNSLLTLKRLTLHADAVVVLDNTALDKIAVERLHLHKPDVQQINSLIATVMAASTTTLRYPGYMNNDLVGLVASLIPTPRCHFLMTGYTPLTAENAAGQVTSNIRKTTVLDVMRRLLQPKNIMVSTHTKSRDIANAKYISILNIIQGEVDPSQVHKSLQRIRERKQANFIEWGPASIQVALSKKSPYVQTAHRVSGLMLANHTSVRHLFNKVLRDYEKLMGPKQERQAFMQAYR 419          
BLAST of CX070097 vs. ExPASy Swiss-Prot
Match: TBG1_HUMAN (Tubulin gamma-1 chain OS=Homo sapiens GN=TUBG1 PE=1 SV=2)

HSP 1 Score: 357.836 bits (917), Expect = 4.274e-98
Identity = 180/237 (75.95%), Postives = 207/237 (87.34%), Query Frame = 3
Query:  138 QMETSDVVVQPYNSLLTLKRLTLNADCVVVLDNTALNRIAVERLHLQNPTFAQTNSLVSTVMSASTTTLRYPGYMNNDLVGLLASLIPTPRCHFLMTGYTPLTVERQANVIRKTTVLDVMRRLLQTKNIMVSSYARTKEASQAKYISILNIIQGEVDPTQVHESLQRIRERKLVNFIEWGPASIQVALSRKSPYVQTAHRVSGLMLASHTSIRHLFSKCLSQYEKLRKKQAFLDNYR 848
            Q E SDVVVQPYNSLLTLKRLT NADCVVVLDNTALNRIA +RLH+QNP+F+Q N LVST+MSASTTTLRYPGYMNNDL+GL+ASLIPTPR HFLMTGYTPLT ++    +RKTTVLDVMRRLLQ KN+MVS+  R ++ +   YI+ILNIIQGEVDPTQVH+SLQRIRERKL NFI WGPASIQVALSRKSPY+ +AHRVSGLM+A+HTSI  LF +   QY+KLRK++AFL+ +R
Sbjct:  175 QDEMSDVVVQPYNSLLTLKRLTQNADCVVVLDNTALNRIATDRLHIQNPSFSQINQLVSTIMSASTTTLRYPGYMNNDLIGLIASLIPTPRLHFLMTGYTPLTTDQSVASVRKTTVLDVMRRLLQPKNVMVST-GRDRQTNHC-YIAILNIIQGEVDPTQVHKSLQRIRERKLANFIPWGPASIQVALSRKSPYLPSAHRVSGLMMANHTSISSLFERTCRQYDKLRKREAFLEQFR 409          
The following BLAST results are available for this feature:
BLAST of CX070097 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 417
Match NameE-valueIdentityDescription
TBG1_ARATH4.382e-12797.89Tubulin gamma-1 chain OS=Arabidopsis thaliana GN=T... [more]
TBG2_ARATH9.762e-12797.05Tubulin gamma-2 chain OS=Arabidopsis thaliana GN=T... [more]
TBG2_ORYSJ2.175e-12697.05Tubulin gamma-2 chain OS=Oryza sativa subsp. japon... [more]
TBG_PHYPA6.327e-12696.20Tubulin gamma chain OS=Physcomitrella patens GN=TU... [more]
TBG3_MAIZE1.410e-12596.20Tubulin gamma-3 chain (Fragment) OS=Zea mays GN=TU... [more]
TBG2_MAIZE1.841e-12595.78Tubulin gamma-2 chain OS=Zea mays GN=TUBG2 PE=2 SV... [more]
TBG1_MAIZE4.101e-12596.20Tubulin gamma-1 chain OS=Zea mays GN=TUBG1 PE=2 SV... [more]
TBG_ANEPH5.922e-12494.96Tubulin gamma chain OS=Anemia phyllitidis GN=TUBG ... [more]
TBG_CHLRE2.050e-10076.73Tubulin gamma chain OS=Chlamydomonas reinhardtii G... [more]
TBG1_HUMAN4.274e-9875.95Tubulin gamma-1 chain OS=Homo sapiens GN=TUBG1 PE=... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS08_14G03_b Parent Washington Navel Orange Callus cDNA Library UCRCS08-2 Citrus sinensis cDNA clone UCRCS08-14G03-M6-1-5.b, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX070097 ID=CX070097; Name=CX070097; organism=Citrus sinensis; type=EST; length=848bp
TTTAAGTGAAATATTTCACTCATGAATTTTGAAGTTTACTGTTTGGTTAT
ATGAATCTTATTTGGATGAATCCATAAGTACTCTGGAAAACAAAAAGACA
AATAAAATGACCGCTCAGTAGGGGCAGATGAACTATGCAGATGGAGACAA
GTGATGTGGTGGTCCAACCGTACAACTCTCTCTTGACACTCAAGCGACTG
ACCCTAAATGCTGATTGTGTTGTTGTTCTTGACAATACTGCACTGAATCG
AATTGCTGTGGAGCGCCTACATCTGCAAAATCCTACATTTGCTCAAACTA
ATTCATTGGTTTCCACTGTAATGTCTGCAAGCACAACCACATTGAGGTAT
CCAGGATACATGAATAATGACTTGGTTGGTCTTCTTGCCTCTCTAATTCC
AACGCCGAGATGCCATTTTCTCATGACAGGATACACACCCCTAACTGTGG
AACGTCAAGCTAATGTGATCCGTAAAACTACTGTACTAGATGTTATGAGA
AGACTGCTGCAGACCAAAAATATCATGGTTTCTTCTTACGCTCGTACAAA
AGAAGCTAGTCAGGCTAAGTACATTTCTATATTGAATATCATTCAAGGAG
AGGTGGACCCTACTCAGGTGCATGAAAGTTTACAGAGGATTCGTGAAAGA
AAGCTTGTCAACTTTATTGAGTGGGGCCCTGCAAGTATTCAGGTTGCTCT
CTCTAGAAAGTCGCCATACGTTCAAACTGCACATAGGGTTAGTGGTCTCA
TGCTGGCAAGCCATACTAGTATCAGGCATCTCTTCAGCAAATGTTTGAGC
CAATATGAGAAGTTGCGAAAAAAGCAAGCCTTTCTTGACAACTACCGA
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