Complete Genome Sequence of Citrus Huanglongbing Bacterium, 'Candidatus Liberibacter asiaticus' Obtained Through Metagenomics

Publication Overview
TitleComplete Genome Sequence of Citrus Huanglongbing Bacterium, 'Candidatus Liberibacter asiaticus' Obtained Through Metagenomics
AuthorsDuan Y, Zhou L, Hall DG, Li W, Doddapaneni H, Lin H, Liu L, Vahling CM, Gabriel DW, Williams KP, Dickerman A, Sun Y, Gottwald T
TypeJournal Article
Journal NameMolecular plant-microbe interactions : MPMI
Volume22
Issue8
Year2009
Page(s)1011-1020
CitationDuan Y, Zhou L, Hall DG, Li W, Doddapaneni H, Lin H, Liu L, Vahling CM, Gabriel DW, Williams KP, Dickerman A, Sun Y, Gottwald T. Complete Genome Sequence of Citrus Huanglongbing Bacterium, 'Candidatus Liberibacter asiaticus' Obtained Through Metagenomics. Molecular plant-microbe interactions : MPMI. 2009; 22(8):1011-1020.

Abstract

Citrus huanglongbing is the most destructive disease of citrus worldwide. It is spread by citrus psyllids and is associated with a low-titer, phloem-limited infection by any of three uncultured species of α-Proteobacteria, 'Candidatus Liberibacter asiaticus', 'Ca. L. americanus', and 'Ca. L. africanus'. A complete circular 'Ca. L. asiaticus' genome has been obtained by metagenomics, using the DNA extracted from a single 'Ca. L. asiaticus'-infected psyllid. The 1.23-Mb genome has an average 36.5% GC content. Annotation revealed a high percentage of genes involved in both cell motility (4.5%) and active transport in general (8.0%), which may contribute to its virulence. 'Ca. L. asiaticus' appears to have a limited ability for aerobic respiration and is likely auxotrophic for at least five amino acids. Consistent with its intracellular nature, 'Ca. L. asiaticus' lacks type III and type IV secretion systems as well as typical free-living or plant-colonizing extracellular degradative enzymes. 'Ca. L. asiaticus' appears to have all type I secretion system genes needed for both multidrug efflux and toxin effector secretion. Multi-protein phylogenetic analysis confirmed 'Ca. L. asiaticus' as an early-branching and highly divergent member of the family Rhizobiaceae. This is the first genome sequence of an uncultured α-proteobacteria that is both an intracellular plant pathogen and insect symbiont.
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Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Publication Date2009
Published Location|||
Language Abbreng
Publication Model[electronic resource].
URLhttp://dx.doi.org/10.1094/MPMI-22-8-1011
KeywordsCandidatus Liberibacter asiaticus, plant pathogenic bacteria, microbial genetics, genome, nucleotide sequences, genomics, DNA, cell motility, active transport, cell respiration, phylogeny, bacterial proteins, Type III secretion system, transport proteins, Psyllidae, symbiosis, symbionts, metagenomics, molecular sequence data
Cross References
This publication is also available in the following databases:
DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:4241273
PMID: PubMedPMID:19589076