DC900350

Overview
NameDC900350
Unique NameDC900350
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length697
Libraries
Library NameType
VSScdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of DC900350 vs. ExPASy Swiss-Prot
Match: RL23A_FRIAG (60S ribosomal protein L23A OS=Fritillaria agrestis GN=RPL23A PE=2 SV=1)

HSP 1 Score: 229.18 bits (583), Expect = 1.650e-59
Identity = 121/154 (78.57%), Postives = 123/154 (79.87%), Query Frame = 3
Query:  102 MAPPKGETKKADPKVQALKTAKAVKSGRTF--KKAKKIRTSVTFHRPKTLKKDRNPKYPRISAPPRNKLDHYQILKYPLTTESAMKKIEDNNTLVFXXXXXXXXXXXXXXXXXMYDIQAKKVNTLIRPDGTKKAYVRLTPDSDALDVANKIGII 557
            MAPP    KK D K QALK AKAVKSG +   KKAKKIRTSVTFHRPKTLKKDRNPKYPRISAPPRNKLDHYQILKYPLTTESAMKKIEDNNTLVF                 MYDIQ KKVNTLIRPDGTKKAYVRLTPD DALD+ANKIGII
Sbjct:    1 MAPPAKVAKKPDGKAQALKVAKAVKSGVSTLQKKAKKIRTSVTFHRPKTLKKDRNPKYPRISAPPRNKLDHYQILKYPLTTESAMKKIEDNNTLVFIVDIRADKKKIKAAVKKMYDIQTKKVNTLIRPDGTKKAYVRLTPDYDALDIANKIGII 154          
BLAST of DC900350 vs. ExPASy Swiss-Prot
Match: RL23A_DAUCA (60S ribosomal protein L23a OS=Daucus carota GN=RPL23A PE=2 SV=1)

HSP 1 Score: 223.787 bits (569), Expect = 6.934e-58
Identity = 120/154 (77.92%), Postives = 124/154 (80.52%), Query Frame = 3
Query:  102 MAPPKGE-TKKADPKVQALKTAKAVKSGRT-FKKAKKIRTSVTFHRPKTLKKDRNPKYPRISAPPRNKLDHYQILKYPLTTESAMKKIEDNNTLVFXXXXXXXXXXXXXXXXXMYDIQAKKVNTLIRPDGTKKAYVRLTPDSDALDVANKIGII 557
            M+P K + TKK+D K QALKTAKAVKSG T FKK KKIRTSVTFHRP+TL KDRNPKYPRISA PRNKLD YQILKYPLTTESAMKKIEDNNTLVF                 MYDIQ KKVNTLIRPDGTKKAYVRLTPD DALDVANKIGII
Sbjct:    1 MSPAKVDVTKKSDAKAQALKTAKAVKSGTTKFKKVKKIRTSVTFHRPRTLTKDRNPKYPRISATPRNKLDQYQILKYPLTTESAMKKIEDNNTLVFIVDIRANKKKIKDAVKKMYDIQTKKVNTLIRPDGTKKAYVRLTPDYDALDVANKIGII 154          
BLAST of DC900350 vs. ExPASy Swiss-Prot
Match: RL23A_TOBAC (60S ribosomal protein L23a OS=Nicotiana tabacum GN=RPL23A PE=2 SV=1)

HSP 1 Score: 219.164 bits (557), Expect = 1.708e-56
Identity = 117/154 (75.97%), Postives = 122/154 (79.22%), Query Frame = 3
Query:  102 MAPPKGE-TKKADPKVQALKTAKAVKSGRTFKK-AKKIRTSVTFHRPKTLKKDRNPKYPRISAPPRNKLDHYQILKYPLTTESAMKKIEDNNTLVFXXXXXXXXXXXXXXXXXMYDIQAKKVNTLIRPDGTKKAYVRLTPDSDALDVANKIGII 557
            MAP K + +KK+DPK QA K AKAVKSG T KK ++KIRT VTFHRPKTLKKDRNPKYPRISAP RNKLD Y ILKYPLTTESAMKKIEDNNTLVF                 MYDIQ KKVNTLIRPDGTKKAYVRLTPD DALDVANKIGII
Sbjct:    1 MAPAKADPSKKSDPKAQAAKVAKAVKSGSTLKKKSQKIRTKVTFHRPKTLKKDRNPKYPRISAPGRNKLDQYGILKYPLTTESAMKKIEDNNTLVFIVDIKADKKKIKDAVKKMYDIQTKKVNTLIRPDGTKKAYVRLTPDYDALDVANKIGII 154          
BLAST of DC900350 vs. ExPASy Swiss-Prot
Match: R23A1_ARATH (60S ribosomal protein L23a-1 OS=Arabidopsis thaliana GN=RPL23AA PE=2 SV=2)

HSP 1 Score: 216.083 bits (549), Expect = 1.446e-55
Identity = 116/154 (75.32%), Postives = 121/154 (78.57%), Query Frame = 3
Query:  102 MAPPKGET-KKADPKVQALKTAKAVKSGRTFKKA-KKIRTSVTFHRPKTLKKDRNPKYPRISAPPRNKLDHYQILKYPLTTESAMKKIEDNNTLVFXXXXXXXXXXXXXXXXXMYDIQAKKVNTLIRPDGTKKAYVRLTPDSDALDVANKIGII 557
            M+P K +T KKADPK +ALK AKAVKSG+ FKK  KKIRT VTFHRPKTL K R  KYP+ISA PRNKLDHYQILKYPLTTESAMKKIEDNNTLVF                 MYDIQ KKVNTLIRPDGTKKAYVRLTPD DALDVANKIGII
Sbjct:    1 MSPAKVDTTKKADPKAKALKAAKAVKSGQAFKKKDKKIRTKVTFHRPKTLTKPRTGKYPKISATPRNKLDHYQILKYPLTTESAMKKIEDNNTLVFIVDIRADKKKIKDAVKKMYDIQTKKVNTLIRPDGTKKAYVRLTPDYDALDVANKIGII 154          
BLAST of DC900350 vs. ExPASy Swiss-Prot
Match: R23A2_ARATH (60S ribosomal protein L23a-2 OS=Arabidopsis thaliana GN=RPL23AB PE=2 SV=1)

HSP 1 Score: 215.698 bits (548), Expect = 1.888e-55
Identity = 116/154 (75.32%), Postives = 121/154 (78.57%), Query Frame = 3
Query:  102 MAPPKGE-TKKADPKVQALKTAKAVKSGRTFKK-AKKIRTSVTFHRPKTLKKDRNPKYPRISAPPRNKLDHYQILKYPLTTESAMKKIEDNNTLVFXXXXXXXXXXXXXXXXXMYDIQAKKVNTLIRPDGTKKAYVRLTPDSDALDVANKIGII 557
            M+P K + TKKADPK +ALK AKAVKSG+  KK AKKIRT VTFHRPKTL   R PKYP+ISA PRNKLDHYQILKYPLTTESAMKKIEDNNTLVF                 MYDIQ KKVNTLIRPDGTKKAYVRLTPD DALDVANKIGII
Sbjct:    1 MSPAKVDVTKKADPKAKALKAAKAVKSGQIVKKPAKKIRTKVTFHRPKTLTVPRKPKYPKISATPRNKLDHYQILKYPLTTESAMKKIEDNNTLVFIVDIRADKKKIKDAVKKMYDIQTKKVNTLIRPDGTKKAYVRLTPDYDALDVANKIGII 154          
BLAST of DC900350 vs. ExPASy Swiss-Prot
Match: RL23A_RAT (60S ribosomal protein L23a OS=Rattus norvegicus GN=Rpl23a PE=2 SV=1)

HSP 1 Score: 179.874 bits (455), Expect = 1.148e-44
Identity = 98/156 (62.82%), Postives = 105/156 (67.31%), Query Frame = 3
Query:   90 RRRTMAPPKGETKKADPKVQALKTAKAVKSGRTFKKAKKIRTSVTFHRPKTLKKDRNPKYPRISAPPRNKLDHYQILKYPLTTESAMKKIEDNNTLVFXXXXXXXXXXXXXXXXXMYDIQAKKVNTLIRPDGTKKAYVRLTPDSDALDVANKIGII 557
            ++   APPK E K      +ALK  KAV  G    K KKIRTS TF RPKTL+  R PKYPR SAP RNKLDHY I+K+PLTTESAMKKIEDNNTLVF                 +YDI   KVNTLIRPDG KKAYVRL PD DALDVANKIGII
Sbjct:    6 KKEAPAPPKAEAK-----AKALKAKKAVLKGVHSHKKKKIRTSPTFRRPKTLRLRRQPKYPRKSAPRRNKLDHYAIIKFPLTTESAMKKIEDNNTLVFIVDVKANKHQIKQAVKKLYDIDVAKVNTLIRPDGEKKAYVRLAPDYDALDVANKIGII 156          
BLAST of DC900350 vs. ExPASy Swiss-Prot
Match: RL23A_MOUSE (60S ribosomal protein L23a OS=Mus musculus GN=Rpl23a PE=2 SV=1)

HSP 1 Score: 179.874 bits (455), Expect = 1.148e-44
Identity = 98/156 (62.82%), Postives = 105/156 (67.31%), Query Frame = 3
Query:   90 RRRTMAPPKGETKKADPKVQALKTAKAVKSGRTFKKAKKIRTSVTFHRPKTLKKDRNPKYPRISAPPRNKLDHYQILKYPLTTESAMKKIEDNNTLVFXXXXXXXXXXXXXXXXXMYDIQAKKVNTLIRPDGTKKAYVRLTPDSDALDVANKIGII 557
            ++   APPK E K      +ALK  KAV  G    K KKIRTS TF RPKTL+  R PKYPR SAP RNKLDHY I+K+PLTTESAMKKIEDNNTLVF                 +YDI   KVNTLIRPDG KKAYVRL PD DALDVANKIGII
Sbjct:    6 KKEAPAPPKAEAK-----AKALKAKKAVLKGVHSHKKKKIRTSPTFRRPKTLRLRRQPKYPRKSAPRRNKLDHYAIIKFPLTTESAMKKIEDNNTLVFIVDVKANKHQIKQAVKKLYDIDVAKVNTLIRPDGEKKAYVRLAPDYDALDVANKIGII 156          
BLAST of DC900350 vs. ExPASy Swiss-Prot
Match: RL23A_HUMAN (60S ribosomal protein L23a OS=Homo sapiens GN=RPL23A PE=1 SV=1)

HSP 1 Score: 179.874 bits (455), Expect = 1.148e-44
Identity = 98/156 (62.82%), Postives = 105/156 (67.31%), Query Frame = 3
Query:   90 RRRTMAPPKGETKKADPKVQALKTAKAVKSGRTFKKAKKIRTSVTFHRPKTLKKDRNPKYPRISAPPRNKLDHYQILKYPLTTESAMKKIEDNNTLVFXXXXXXXXXXXXXXXXXMYDIQAKKVNTLIRPDGTKKAYVRLTPDSDALDVANKIGII 557
            ++   APPK E K      +ALK  KAV  G    K KKIRTS TF RPKTL+  R PKYPR SAP RNKLDHY I+K+PLTTESAMKKIEDNNTLVF                 +YDI   KVNTLIRPDG KKAYVRL PD DALDVANKIGII
Sbjct:    6 KKEAPAPPKAEAK-----AKALKAKKAVLKGVHSHKKKKIRTSPTFRRPKTLRLRRQPKYPRKSAPRRNKLDHYAIIKFPLTTESAMKKIEDNNTLVFIVDVKANKHQIKQAVKKLYDIDVAKVNTLIRPDGEKKAYVRLAPDYDALDVANKIGII 156          
BLAST of DC900350 vs. ExPASy Swiss-Prot
Match: RL23A_BOVIN (60S ribosomal protein L23a OS=Bos taurus GN=RPL23A PE=2 SV=1)

HSP 1 Score: 179.874 bits (455), Expect = 1.148e-44
Identity = 98/156 (62.82%), Postives = 105/156 (67.31%), Query Frame = 3
Query:   90 RRRTMAPPKGETKKADPKVQALKTAKAVKSGRTFKKAKKIRTSVTFHRPKTLKKDRNPKYPRISAPPRNKLDHYQILKYPLTTESAMKKIEDNNTLVFXXXXXXXXXXXXXXXXXMYDIQAKKVNTLIRPDGTKKAYVRLTPDSDALDVANKIGII 557
            ++   APPK E K      +ALK  KAV  G    K KKIRTS TF RPKTL+  R PKYPR SAP RNKLDHY I+K+PLTTESAMKKIEDNNTLVF                 +YDI   KVNTLIRPDG KKAYVRL PD DALDVANKIGII
Sbjct:    6 KKEAPAPPKAEAK-----AKALKAKKAVLKGVHSHKKKKIRTSPTFRRPKTLRLRRQPKYPRKSAPRRNKLDHYAIIKFPLTTESAMKKIEDNNTLVFIVDVKANKHQIKQAVKKLYDIDVAKVNTLIRPDGEKKAYVRLAPDYDALDVANKIGII 156          
BLAST of DC900350 vs. ExPASy Swiss-Prot
Match: RL25_PUCGR (60S ribosomal protein L25 OS=Puccinia graminis PE=2 SV=2)

HSP 1 Score: 154.066 bits (388), Expect = 6.747e-37
Identity = 84/158 (53.16%), Postives = 100/158 (63.29%), Query Frame = 3
Query:  108 PPKGETKKADPKVQALKTA--------KAVKSGRTFKKAKKIRTSVTFHRPKTLKKDRNPKYPRISAPPRNKLDHYQILKYPLTTESAMKKIEDNNTLVFXXXXXXXXXXXXXXXXXMYDIQAKKVNTLIRPDGTKKAYVRLTPDSDALDVANKIGII 557
            PPK  TK A+PK  + KT         KAV  G + K  ++IRTSVTF RPKTL+  R PKYPR S P   ++D Y+ L  PL TESAMKKIEDNNTL+F                 +YD+   +VNTLIRPDG KKA+VRLTP+ DALD+ANKIG I
Sbjct:    2 PPKSSTK-AEPKASSAKTQVAKAKSAKKAVVKGTSSKTQRRIRTSVTFRRPKTLRLSRKPKYPRTSVPHAPRMDAYRTLVRPLNTESAMKKIEDNNTLLFIVDLKANKRQIADAVKKLYDVTPLRVNTLIRPDGKKKAFVRLTPEVDALDIANKIGFI 158          
The following BLAST results are available for this feature:
BLAST of DC900350 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 20
Match NameE-valueIdentityDescription
RL23A_FRIAG1.650e-5978.5760S ribosomal protein L23A OS=Fritillaria agrestis... [more]
RL23A_DAUCA6.934e-5877.9260S ribosomal protein L23a OS=Daucus carota GN=RPL... [more]
RL23A_TOBAC1.708e-5675.9760S ribosomal protein L23a OS=Nicotiana tabacum GN... [more]
R23A1_ARATH1.446e-5575.3260S ribosomal protein L23a-1 OS=Arabidopsis thalia... [more]
R23A2_ARATH1.888e-5575.3260S ribosomal protein L23a-2 OS=Arabidopsis thalia... [more]
RL23A_RAT1.148e-4462.8260S ribosomal protein L23a OS=Rattus norvegicus GN... [more]
RL23A_MOUSE1.148e-4462.8260S ribosomal protein L23a OS=Mus musculus GN=Rpl2... [more]
RL23A_HUMAN1.148e-4462.8260S ribosomal protein L23a OS=Homo sapiens GN=RPL2... [more]
RL23A_BOVIN1.148e-4462.8260S ribosomal protein L23a OS=Bos taurus GN=RPL23A... [more]
RL25_PUCGR6.747e-3753.1660S ribosomal protein L25 OS=Puccinia graminis PE=... [more]

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Properties
Property NameValue
Genbank descriptionDC900350 VSS Citrus sinensis cDNA clone VS28909 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DC900350 ID=DC900350|Name=DC900350|organism=Citrus sinensis|type=EST|length=697bp
CTTTTTTATAAACCCTAGTTTCCTCTTCACAAAGACCAAATCCCAGAGCC
GGCTCCTCTACTGCCCACTTACAGGTAAGTTCTCATCATCGCCGGCGAAC
AATGGCTCCTCCAAAAGGTGAGACAAAAAAGGCTGATCCAAAGGTTCAGG
CCTTGAAGACTGCGAAAGCAGTGAAGTCTGGCCGTACCTTTAAGAAGGCC
AAGAAGATCCGGACTTCAGTTACATTTCACCGTCCAAAGACATTGAAGAA
GGACCGGAATCCCAAGTACCCTCGCATCAGTGCTCCACCTAGGAACAAGC
TTGACCATTATCAGATTCTCAAGTACCCACTCACCACTGAATCAGCAATG
AAGAAAATTGAAGACAACAATACTCTGGTTTTCATTGTTGACATACGGGC
TGATAAGAAAAAGATAAAGGATGCAGTTAAGAAGATGTATGATATCCAGG
CTAAGAAAGTGAATACCTTGATCAGGCCCGATGGCACAAAGAAGGCGTAT
GTCAGGTTGACACCAGACTCTGATGCTTTGGACGTAGCAAACAAGATTGG
AATCATTTAAACAATTGATCTTTCATTCACATTTTTGTGTATTTTTTAAT
CCAAAAAGTTAGTTATGTTAAGACCTCAAACAAAGATTTGCAAATTTGTT
CCTGTTAACATTTGTTACTGCTGATTAATATTTTCCTATTTTGTGTT
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