CN185974

Overview
NameCN185974
Unique NameCN185974
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length710
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CN185974_ssr497 microsatellite CN185974_ssr497:497..508. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig2513 contig Csv1_Contig2513:1..710. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CN185974 vs. ExPASy Swiss-Prot
Match: IF4E1_ARATH (Eukaryotic translation initiation factor 4E-1 OS=Arabidopsis thaliana GN=EIF4E1 PE=2 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 3.260e-58
Identity = 61/79 (77.22%), Postives = 70/79 (88.61%), Query Frame = -2 
Query:  197 LLATIGEQFDHGDEICGAVVNVRARQEKISLWTKNASNEAAQMSIGKQWKELLDYSDTIGFIFHEDAKH-DNRSKNRYT 430
            LLA IGEQFDHGDEICGAVVN+R +QE+IS+WTKNASNEAAQ+SIGKQWKE LDY+++IGFI HEDAK  D  +KN YT
Sbjct:  156 LLALIGEQFDHGDEICGAVVNIRGKQERISIWTKNASNEAAQVSIGKQWKEFLDYNNSIGFIIHEDAKKLDRNAKNAYT 234

HSP 2 Score: 117.087 bits (292), Expect = 3.260e-58
Identity = 49/60 (81.67%), Postives = 54/60 (90.00%), Query Frame = -3 
Query:  529 EFWSLYNNIHHPSKLAVGADFYCFKNKIEPKWEDPVCANGGKWTVIFPKGKSDTSWLYTV 708
            EFWSLYNN+ HPSKLA GADFYCFK+ IEPKWEDP+CANGGKWT+ FPK KSD SWLYT+
Sbjct:   97 EFWSLYNNMKHPSKLAHGADFYCFKHIIEPKWEDPICANGGKWTMTFPKEKSDKSWLYTL 156
[Close]
BLAST of CN185974 vs. ExPASy Swiss-Prot
Match: IF4E1_ORYSJ (Eukaryotic translation initiation factor 4E-1 OS=Oryza sativa subsp. japonica GN=Os01g0970400 PE=2 SV=1)

HSP 1 Score: 124.405 bits (311), Expect = 1.337e-56
Identity = 59/79 (74.68%), Postives = 69/79 (87.34%), Query Frame = -2 
Query:  197 LLATIGEQFDHGDEICGAVVNVRARQEKISLWTKNASNEAAQMSIGKQWKELLDYSDTIGFIFHEDAKH-DNRSKNRYT 430
            LLA IGEQFD+GDEICGAVV+VR +QE+I++WTKNA+NEAAQ+SIGKQWKE LDY D+IGFI H+DAK  D   KNRYT
Sbjct:  148 LLAMIGEQFDYGDEICGAVVSVRGKQERIAIWTKNAANEAAQISIGKQWKEFLDYKDSIGFIVHDDAKKMDKGLKNRYT 226

HSP 2 Score: 117.472 bits (293), Expect = 1.337e-56
Identity = 48/60 (80.00%), Postives = 54/60 (90.00%), Query Frame = -3 
Query:  529 EFWSLYNNIHHPSKLAVGADFYCFKNKIEPKWEDPVCANGGKWTVIFPKGKSDTSWLYTV 708
            +FWSLYNNIHHPSKL VGADF+CFKNKIEPKWEDP+CANGGKWT    +GKSDT WL+T+
Sbjct:   89 DFWSLYNNIHHPSKLVVGADFHCFKNKIEPKWEDPICANGGKWTFSCGRGKSDTMWLHTL 148
[Close]
BLAST of CN185974 vs. ExPASy Swiss-Prot
Match: IF4E1_MAIZE (Eukaryotic translation initiation factor 4E-1 OS=Zea mays PE=2 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 1.116e-55
Identity = 59/79 (74.68%), Postives = 69/79 (87.34%), Query Frame = -2 
Query:  197 LLATIGEQFDHGDEICGAVVNVRARQEKISLWTKNASNEAAQMSIGKQWKELLDYSDTIGFIFHEDAKH-DNRSKNRYT 430
            LLA IGEQFD+GDEICGAVV+VR +QE+I++WTKNA+NEAAQ+SIGKQWKELLDY D+IGFI H+DAK  D   K RYT
Sbjct:  139 LLAMIGEQFDYGDEICGAVVSVRGKQERIAIWTKNAANEAAQVSIGKQWKELLDYKDSIGFIVHDDAKKMDKGLKERYT 217

HSP 2 Score: 115.161 bits (287), Expect = 1.116e-55
Identity = 47/60 (78.33%), Postives = 54/60 (90.00%), Query Frame = -3 
Query:  529 EFWSLYNNIHHPSKLAVGADFYCFKNKIEPKWEDPVCANGGKWTVIFPKGKSDTSWLYTV 708
            EFW LYNNI+HPSKL VGADF+CFKNKIEPKWEDP+CANGGKWT+   +GKSDT WL+T+
Sbjct:   80 EFWGLYNNINHPSKLIVGADFHCFKNKIEPKWEDPICANGGKWTISCGRGKSDTFWLHTL 139
[Close]
BLAST of CN185974 vs. ExPASy Swiss-Prot
Match: IF4E1_WHEAT (Eukaryotic translation initiation factor 4E-1 OS=Triticum aestivum PE=1 SV=3)

HSP 1 Score: 124.405 bits (311), Expect = 1.898e-55
Identity = 59/79 (74.68%), Postives = 68/79 (86.08%), Query Frame = -2 
Query:  197 LLATIGEQFDHGDEICGAVVNVRARQEKISLWTKNASNEAAQMSIGKQWKELLDYSDTIGFIFHEDAKH-DNRSKNRYT 430
            LLA IGEQFD GDEICGAVV+VR +QE++++WTKNA+NEAAQ+SIGKQWKE LDY D+IGFI HEDAK  D   KNRYT
Sbjct:  136 LLAMIGEQFDFGDEICGAVVSVRQKQERVAIWTKNAANEAAQISIGKQWKEFLDYKDSIGFIVHEDAKRSDKGPKNRYT 214

HSP 2 Score: 113.62 bits (283), Expect = 1.898e-55
Identity = 46/60 (76.67%), Postives = 54/60 (90.00%), Query Frame = -3 
Query:  529 EFWSLYNNIHHPSKLAVGADFYCFKNKIEPKWEDPVCANGGKWTVIFPKGKSDTSWLYTV 708
            +FW LYNNIH+PSKL VGADF+CFKNKIEPKWEDP+CANGGKWT+   +GKSDT WL+T+
Sbjct:   77 DFWGLYNNIHNPSKLNVGADFHCFKNKIEPKWEDPICANGGKWTISCGRGKSDTFWLHTL 136
[Close]
BLAST of CN185974 vs. ExPASy Swiss-Prot
Match: IF4E4_ARATH (Putative eukaryotic translation initiation factor 4E-4 OS=Arabidopsis thaliana GN=At1g29550 PE=2 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 2.031e-51
Identity = 60/85 (70.59%), Postives = 69/85 (81.18%), Query Frame = -2 
Query:  197 CTW*--LLATIGEQFDHGDEICGAVVNVRARQEKISLWTKNASNEAAQMSIGKQWKELLDYSDTIGFIFHEDAKH-DNRSKNRYT 442
            C W   LLA +GEQFD GDEICGAV+N RAR ++ISLWTKNA+NE AQ+SIGKQWKELL Y++TIGFI HEDAK  D  +K RYT
Sbjct:  155 CNWLNTLLALVGEQFDQGDEICGAVLNFRARGDRISLWTKNAANEEAQLSIGKQWKELLGYNETIGFIVHEDAKTLDRDAKRRYT 239

HSP 2 Score: 103.219 bits (256), Expect = 2.031e-51
Identity = 41/60 (68.33%), Postives = 50/60 (83.33%), Query Frame = -3 
Query:  529 EFWSLYNNIHHPSKLAVGADFYCFKNKIEPKWEDPVCANGGKWTVIFPKGKSDTSWLYTV 708
            EFWSLYNNIH P+K   GAD YCFK+KIEPKWEDP+CANGGKW+++FPK   + +WL T+
Sbjct:  102 EFWSLYNNIHPPTKWVSGADLYCFKDKIEPKWEDPICANGGKWSMMFPKATLECNWLNTL 161
[Close]
BLAST of CN185974 vs. ExPASy Swiss-Prot
Match: IF4E5_ARATH (Eukaryotic translation initiation factor 4E-5 OS=Arabidopsis thaliana GN=At1g29590 PE=2 SV=2)

HSP 1 Score: 117.857 bits (294), Expect = 2.876e-50
Identity = 57/79 (72.15%), Postives = 65/79 (82.28%), Query Frame = -2 
Query:  197 LLATIGEQFDHGDEICGAVVNVRARQEKISLWTKNASNEAAQMSIGKQWKELLDYSDTIGFIFHEDAKH-DNRSKNRYT 430
            LLA +GEQFD GDEICGAV+N R R ++ISLWTK A+NE AQ+SIGKQWKELL Y+DTIGFI HEDAK  D  +K RYT
Sbjct:  161 LLALVGEQFDQGDEICGAVLNFRTRGDRISLWTKKAANEEAQLSIGKQWKELLGYNDTIGFIVHEDAKTLDRDAKRRYT 239

HSP 2 Score: 102.834 bits (255), Expect = 2.876e-50
Identity = 40/60 (66.67%), Postives = 50/60 (83.33%), Query Frame = -3 
Query:  529 EFWSLYNNIHHPSKLAVGADFYCFKNKIEPKWEDPVCANGGKWTVIFPKGKSDTSWLYTV 708
            EFWSLYNNIH P+K   G+D YCFK+KIEPKWEDP+CANGGKWT+ FP+   +++WL T+
Sbjct:  102 EFWSLYNNIHPPTKWVSGSDLYCFKDKIEPKWEDPICANGGKWTMFFPRATLESNWLNTL 161
[Close]
BLAST of CN185974 vs. ExPASy Swiss-Prot
Match: IF4E2_MAIZE (Eukaryotic translation initiation factor 4E-2 OS=Zea mays PE=2 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 9.862e-38
Identity = 44/79 (55.70%), Postives = 63/79 (79.75%), Query Frame = -2 
Query:  197 LLATIGEQFDHGDEICGAVVNVRARQEKISLWTKNASNEAAQMSIGKQWKELLDYSDTIGFIFHEDAKHDNRSK-NRYT 430
            L+A IGEQFD  ++ICG V +VRAR +K++LWT+ ASNEA Q++IGK+WK+++DY+D I + FH+D+K D  S+  RYT
Sbjct:  137 LMALIGEQFDETEDICGIVASVRARGDKLALWTRTASNEAVQVNIGKKWKDVIDYNDKITYTFHDDSKRDKPSRGGRYT 215

HSP 2 Score: 78.9518 bits (193), Expect = 9.862e-38
Identity = 36/61 (59.02%), Postives = 43/61 (70.49%), Query Frame = -3 
Query:  529 EFWSLYNNIHHPSKLAVGADFYCFKNKIEPKWEDPVCANGGKWTV-IFPKGKSDTSWLYTV 708
            EFWS+Y+ I  PSKL+  ADF+ FK  +EPKWEDP CANGGKWTV    K   +T WL T+
Sbjct:   77 EFWSMYDQIFRPSKLSGNADFHLFKAGVEPKWEDPECANGGKWTVPCNRKATFETMWLETL 137
[Close]
BLAST of CN185974 vs. ExPASy Swiss-Prot
Match: IF4E2_WHEAT (Eukaryotic translation initiation factor 4E-2 OS=Triticum aestivum PE=2 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 9.868e-38
Identity = 44/78 (56.41%), Postives = 59/78 (75.64%), Query Frame = -2 
Query:  197 LATIGEQFDHGDEICGAVVNVRARQEKISLWTKNASNEAAQMSIGKQWKELLDYSDTIGFIFHEDAKHDNRSK-NRYT 427
            +A IGEQFD   EICG V +VR RQ+K+SLWTK ASNEA Q+ IGK+WKE++DY+D + + FH+D++    S+  RYT
Sbjct:  131 MALIGEQFDESQEICGVVASVRQRQDKLSLWTKTASNEAVQVDIGKKWKEVIDYNDKMVYSFHDDSRSQKPSRGGRYT 208

HSP 2 Score: 80.8777 bits (198), Expect = 9.868e-38
Identity = 38/60 (63.33%), Postives = 41/60 (68.33%), Query Frame = -3 
Query:  532 EFWSLYNNIHHPSKLAVGADFYCFKNKIEPKWEDPVCANGGKWTVIFP-KGKSDTSWLYT 708
            EFW LY+ I  PSKL   ADF+ FK  +EPKWEDP CANGGKWTVI   K   DT WL T
Sbjct:   70 EFWCLYDQIFRPSKLVGSADFHLFKAGVEPKWEDPECANGGKWTVISSRKTNLDTMWLET 129
[Close]
BLAST of CN185974 vs. ExPASy Swiss-Prot
Match: IF4E2_ORYSJ (Eukaryotic translation initiation factor 4E-2 OS=Oryza sativa subsp. japonica GN=Os10g0467600 PE=2 SV=2)

HSP 1 Score: 97.0561 bits (240), Expect = 2.358e-36
Identity = 41/74 (55.41%), Postives = 59/74 (79.73%), Query Frame = -2 
Query:  209 LLATIGEQFDHGDEICGAVVNVRARQEKISLWTKNASNEAAQMSIGKQWKELLDYSDTIGFIFHEDAKHDNRSK 430
            L+A IGEQFD  +EICG V +VR R +K++LWT+ ASNEA Q++IGK+WKE++DY+D + + FH+DAK +  S+
Sbjct:  127 LMALIGEQFDESEEICGVVASVRQRGDKLALWTRTASNEAVQVNIGKKWKEIVDYNDKMVYSFHDDAKREKPSR 200

HSP 2 Score: 77.0258 bits (188), Expect = 2.358e-36
Identity = 35/61 (57.38%), Postives = 41/61 (67.21%), Query Frame = -3 
Query:  529 EFWSLYNNIHHPSKLAVGADFYCFKNKIEPKWEDPVCANGGKWTV-IFPKGKSDTSWLYTV 708
            EFW LY+ I  PSK+ V ADF+ FK  +EPKWEDP CANGGKWTV    K   +  WL T+
Sbjct:   67 EFWGLYDQIFRPSKVTVNADFHLFKAGVEPKWEDPECANGGKWTVPCSRKTTLENMWLETL 127
[Close]
BLAST of CN185974 vs. ExPASy Swiss-Prot
Match: IF4E2_ARATH (Eukaryotic translation initiation factor 4E-2 OS=Arabidopsis thaliana GN=EIF4E2 PE=1 SV=2)

HSP 1 Score: 85.8853 bits (211), Expect = 4.834e-30
Identity = 40/70 (57.14%), Postives = 53/70 (75.71%), Query Frame = -2 
Query:  227 LLATIGEQFDHGDEICGAVVNVR--ARQEKISLWTKNASNEAAQMSIGKQWKELLDYSDTIGFIFHEDAK 430
            L+A IGEQFD  DEICG V +VR  ++Q+K+SLWT+  SNEA  M IGK+WKE+LD +D I F  H+D++
Sbjct:  123 LMALIGEQFDEADEICGVVASVRPQSKQDKLSLWTRTKSNEAVLMGIGKKWKEILDVTDKITFNNHDDSR 192

HSP 2 Score: 67.0106 bits (162), Expect = 4.834e-30
Identity = 29/63 (46.03%), Postives = 37/63 (58.73%), Query Frame = -3 
Query:  529 EFWSLYNNIHHPSKLAVGADFYCFKNKIEPKWEDPVCANGGKWTVIFPKGKS---DTSWLYTV 708
            +FW L+  I   SKL   A+ + FK  +EPKWEDP CANGGKWT +    +    D  WL T+
Sbjct:   61 DFWGLHETIFQTSKLTANAEIHLFKAGVEPKWEDPECANGGKWTWVVTANRKEALDKGWLETL 123
The following BLAST results are available for this feature:
BLAST of CN185974 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 24
Match NameE-valueIdentityDescription
IF4E1_ARATH3.260e-5877.22Eukaryotic translation initiation factor 4E-1 OS=A... [more]
IF4E1_ORYSJ1.337e-5674.68Eukaryotic translation initiation factor 4E-1 OS=O... [more]
IF4E1_MAIZE1.116e-5574.68Eukaryotic translation initiation factor 4E-1 OS=Z... [more]
IF4E1_WHEAT1.898e-5574.68Eukaryotic translation initiation factor 4E-1 OS=T... [more]
IF4E4_ARATH2.031e-5170.59Putative eukaryotic translation initiation factor ... [more]
IF4E5_ARATH2.876e-5072.15Eukaryotic translation initiation factor 4E-5 OS=A... [more]
IF4E2_MAIZE9.862e-3855.70Eukaryotic translation initiation factor 4E-2 OS=Z... [more]
IF4E2_WHEAT9.868e-3856.41Eukaryotic translation initiation factor 4E-2 OS=T... [more]
IF4E2_ORYSJ2.358e-3655.41Eukaryotic translation initiation factor 4E-2 OS=O... [more]
IF4E2_ARATH4.834e-3057.14Eukaryotic translation initiation factor 4E-2 OS=A... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0002C16_f Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0002C16, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN185974 ID=CN185974; Name=CN185974; organism=Citrus sinensis; type=EST; length=710bp
TTTTTTTAACAACTTCAATATATTGCAAAGGTAAAATCAGCTAGTGCTTC
CCATTGGCGTTTAAGAGGGTATTAGTACAGAAAACAGGAAATCGTAAAAG
GTTTCAGAGCTCTATAACAACTAACAAGCCTACTGTCATCCAGAAAATAC
AATGCATTTCCTGGGACTACACTGCAAAACTCTGTACAGTTTATGTTGTG
TAGCGATTCTTTGACCTGTTGTCATGCTTTGCATCCTCATGAAAGATGAA
CCCTATGGTGTCACTATAATCAAGCAACTCCTTCCATTGTTTCCCAATGC
TCATCTGAGCAGCTTCATTTGAAGCATTCTTGGTCCACAGAGATATCTTT
TCCTGCCTAGCTCTAACATTTACAACTGCTCCACAGATTTCATCTCCATG
ATCAAACTGTTCTCCAATGGTTGCCAGCAACTACCAAGTACATGTACAAA
TTTAAACCCATACTTGGCATTTAATATTAGGCTGATAAACCAACATAAAC
AAACAAACCCACTCATTGAGTCAAGACATACCGTATACAGCCAAGAAGTA
TCAGATTTTCCTTTAGGAAAGATGACAGTCCACTTCCCGCCATTAGCACA
AACTGGGTCCTCCCATTTTGGCTCAATCTTATTCTTGAAACAATAAAAGT
CTGCTCCCACAGCCAACTTGCTCGGATGATGTATATTGTTGTAAAGGCTC
CAGAACTCTT
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