CN185134

Overview
NameCN185134
Unique NameCN185134
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length756
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig2462 contig Csv1_Contig2462:1..756. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_CAMAC (Tryptophan synthase beta chain 2, chloroplastic OS=Camptotheca acuminata GN=TSB PE=2 SV=1)

HSP 1 Score: 215.698 bits (548), Expect = 3.845e-64
Identity = 101/135 (74.81%), Postives = 117/135 (86.67%), Query Frame = -1 
Query:  247 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVNY 651
            +EAAGFGLDSGKHAATL KGEVGV HGAMSYLLQD++GQI+  HS+  GL+YPGVGPE SFL+D GRAE+Y  TD+EA++A++RL RLEGI PALE SHALAFLEKLCPTLPNG KVV+NCSG GDKDV T + +
Sbjct:  329 VEAAGFGLDSGKHAATLTKGEVGVLHGAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSFLKDIGRAEYYCCTDEEALEAFKRLSRLEGIIPALETSHALAFLEKLCPTLPNGTKVVLNCSGRGDKDVHTAIKH 463

HSP 2 Score: 38.891 bits (89), Expect = 3.845e-64
Identity = 15/20 (75.00%), Postives = 20/20 (100.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFHEF++D+DVR+IGV
Sbjct:  310 NAMGLFHEFVDDKDVRMIGV 329

HSP 3 Score: 33.113 bits (74), Expect = 3.845e-64
Identity = 13/19 (68.42%), Postives = 15/19 (78.95%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            GGKPDVL+ CVG GS  +G
Sbjct:  295 GGKPDVLVACVGGGSNAMG 313
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRBP2_ARATH (Tryptophan synthase beta chain 2, chloroplastic OS=Arabidopsis thaliana GN=TSB2 PE=2 SV=2)

HSP 1 Score: 206.838 bits (525), Expect = 8.083e-61
Identity = 96/135 (71.11%), Postives = 117/135 (86.67%), Query Frame = -1 
Query:  247 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVNY 651
            +EAAGFGLDSGKHAATL KG+VGV HGAMSYLLQD++GQI+  HS+  GL+YPGVGPE SFL+D GRAE+++ TD+EA++A++R+ RLEGI PALE SHALA LEKLCPTLP+GA+VV+N SG GDKDV T + Y
Sbjct:  338 VEAAGFGLDSGKHAATLTKGDVGVLHGAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSFLKDVGRAEYFSVTDEEALEAFKRVSRLEGIIPALETSHALAHLEKLCPTLPDGARVVLNFSGRGDKDVQTAIKY 472

HSP 2 Score: 36.5798 bits (83), Expect = 8.083e-61
Identity = 14/20 (70.00%), Postives = 19/20 (95.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFHEF++D +VR+IGV
Sbjct:  319 NAMGLFHEFVDDTEVRMIGV 338

HSP 3 Score: 33.113 bits (74), Expect = 8.083e-61
Identity = 13/19 (68.42%), Postives = 15/19 (78.95%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            GGKPDVL+ CVG GS  +G
Sbjct:  304 GGKPDVLVACVGGGSNAMG 322
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB1_MAIZE (Tryptophan synthase beta chain 1 (Fragment) OS=Zea mays GN=TSB1 PE=2 SV=1)

HSP 1 Score: 197.208 bits (500), Expect = 7.179e-59
Identity = 91/135 (67.41%), Postives = 113/135 (83.70%), Query Frame = -1 
Query:  247 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVNY 651
            +EAAG G+D+ KHAATL KG+VGV HG+MSYLLQD++GQ++  HS+  GL+YPGVGPE SFL+D GRAE+ + TDQEA+ A++R+ RLEGI PALE SHALA+LEKLCPTLP+G +VV+NCSG GDKDV T   Y
Sbjct:  252 VEAAGHGVDTDKHAATLTKGQVGVLHGSMSYLLQDDDGQVIEPHSISAGLDYPGVGPEHSFLKDIGRAEYDSVTDQEALDAFKRVSRLEGIIPALETSHALAYLEKLCPTLPDGVRVVLNCSGRGDKDVHTASKY 386

HSP 2 Score: 39.6614 bits (91), Expect = 7.179e-59
Identity = 16/20 (80.00%), Postives = 19/20 (95.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFHEF+ D+DVRLIGV
Sbjct:  233 NAMGLFHEFVEDQDVRLIGV 252

HSP 3 Score: 33.113 bits (74), Expect = 7.179e-59
Identity = 13/19 (68.42%), Postives = 15/19 (78.95%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            GGKPDVL+ CVG GS  +G
Sbjct:  218 GGKPDVLVACVGGGSNAMG 236
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB2_MAIZE (Tryptophan synthase beta chain 2, chloroplastic (Fragment) OS=Zea mays GN=TSB2 PE=2 SV=1)

HSP 1 Score: 196.438 bits (498), Expect = 3.484e-58
Identity = 92/135 (68.15%), Postives = 112/135 (82.96%), Query Frame = -1 
Query:  247 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVNY 651
            LEAAG G+D+ KHAATL KG+VGV HG+MSYLLQD++GQ++  HS+  GL+YPGVGPE SFL+D GRAE+ + TDQEA+ A++R+ RLEGI PALE SHALA+LEKLCPTL +G +VVVNCSG GDKDV T   Y
Sbjct:  306 LEAAGHGVDTDKHAATLTKGQVGVLHGSMSYLLQDDDGQVIEPHSISAGLDYPGVGPEHSFLKDIGRAEYDSVTDQEALDAFKRVSRLEGIIPALETSHALAYLEKLCPTLADGVRVVVNCSGRGDKDVHTASKY 440

HSP 2 Score: 38.1206 bits (87), Expect = 3.484e-58
Identity = 14/20 (70.00%), Postives = 19/20 (95.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFHEF+ D+DVRL+G+
Sbjct:  287 NAMGLFHEFVEDQDVRLVGL 306

HSP 3 Score: 33.113 bits (74), Expect = 3.484e-58
Identity = 13/19 (68.42%), Postives = 15/19 (78.95%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            GGKPDVL+ CVG GS  +G
Sbjct:  272 GGKPDVLVACVGGGSNAMG 290
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB1_ARATH (Tryptophan synthase beta chain 1, chloroplastic OS=Arabidopsis thaliana GN=TSB1 PE=2 SV=1)

HSP 1 Score: 203.756 bits (517), Expect = 3.030e-55
Identity = 98/149 (65.77%), Postives = 122/149 (81.88%), Query Frame = -1 
Query:  247 FMNS*MMKM*D*LELEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVNY 693
            F+N   ++M   + +EAAGFGLDSGKHAATL KG+VGV HGAMSYLLQD++GQI+  HS+  GL+YPGVGPE SF +D GRAE+Y+ TD+EA++A++R+ RLEGI PALE SHALA+LEKLCPTL +G +VV+N SG GDKDV TV  Y
Sbjct:  322 FVNDTEVRM---IGVEAAGFGLDSGKHAATLTKGDVGVLHGAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSFFKDMGRAEYYSITDEEALEAFKRVSRLEGIIPALETSHALAYLEKLCPTLSDGTRVVLNFSGRGDKDVQTVAKY 467

HSP 2 Score: 33.113 bits (74), Expect = 3.030e-55
Identity = 13/19 (68.42%), Postives = 15/19 (78.95%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            GGKPDVL+ CVG GS  +G
Sbjct:  299 GGKPDVLVACVGGGSNAMG 317
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_ANAVT (Tryptophan synthase beta chain OS=Anabaena variabilis (strain ATCC 29413 / PCC 7937) GN=trpB PE=3 SV=1)

HSP 1 Score: 187.578 bits (475), Expect = 5.986e-54
Identity = 84/135 (62.22%), Postives = 109/135 (80.74%), Query Frame = -1 
Query:  247 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVNY 651
            +EAAG G+++ KHAATL KG VGV HGAMSYLLQDE+GQ++  HS+  GL+YPGVGPE S+L+D GRAE+Y+ TD++A+ A+QRL RLEGI PALE +HA+A+LE LCP L    ++++NCSG GDKDV TV  +
Sbjct:  275 VEAAGEGVNTEKHAATLTKGRVGVLHGAMSYLLQDEDGQVIEAHSISAGLDYPGVGPEHSYLKDVGRAEYYSVTDEQALAAFQRLSRLEGIIPALETAHAIAYLETLCPQLDGSPRIIINCSGRGDKDVQTVAKF 409

HSP 2 Score: 34.6538 bits (78), Expect = 5.986e-54
Identity = 13/20 (65.00%), Postives = 18/20 (90.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLF+EF+N+  +RLIGV
Sbjct:  256 NAMGLFYEFVNESSIRLIGV 275

HSP 3 Score: 31.187 bits (69), Expect = 5.986e-54
Identity = 12/19 (63.16%), Postives = 14/19 (73.68%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            GG PD+LL CVG GS  +G
Sbjct:  241 GGLPDILLACVGGGSNAMG 259
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_THEEB (Tryptophan synthase beta chain OS=Thermosynechococcus elongatus (strain BP-1) GN=trpB PE=3 SV=1)

HSP 1 Score: 186.808 bits (473), Expect = 1.014e-53
Identity = 88/135 (65.19%), Postives = 107/135 (79.26%), Query Frame = -1 
Query:  247 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVNY 651
            +EAAG GLD+G HAATL KGEVGV HGAMSYLLQD +GQ++  HS+  GL+YPGVGPE S+L+D GRAE+Y+ TD EAV A  RL +LEGI PALE +HALA+LE LCP L    ++V+NCSG GDKDV+T+  Y
Sbjct:  272 VEAAGQGLDTGHHAATLTKGEVGVLHGAMSYLLQDADGQVVEAHSISAGLDYPGVGPEHSYLKDIGRAEYYSVTDTEAVAACVRLAQLEGILPALETAHALAYLETLCPQLTGQPRIVINCSGRGDKDVETIGRY 406

HSP 2 Score: 34.6538 bits (78), Expect = 1.014e-53
Identity = 14/20 (70.00%), Postives = 17/20 (85.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFHEF+ +  VRLIGV
Sbjct:  253 NAMGLFHEFVEEPQVRLIGV 272

HSP 3 Score: 31.187 bits (69), Expect = 1.014e-53
Identity = 12/19 (63.16%), Postives = 14/19 (73.68%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            GG PD+LL CVG GS  +G
Sbjct:  238 GGLPDILLACVGGGSNAMG 256
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB_SYNY3 (Tryptophan synthase beta chain OS=Synechocystis sp. (strain PCC 6803) GN=trpB PE=3 SV=1)

HSP 1 Score: 188.734 bits (478), Expect = 3.783e-53
Identity = 88/135 (65.19%), Postives = 106/135 (78.52%), Query Frame = -1 
Query:  247 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVNY 651
            +EAAG  + SGKHAATL  G+ GV HGAMSYLLQD+EGQ+   HS+  GL+YPGVGPE S+L+D GRAE+Y+ TDQEA+ A QRL +LEGI PALE +HA A+LE LCP L NG ++V+NCSG GDKDV TV  Y
Sbjct:  273 IEAAGESIVSGKHAATLTMGKPGVLHGAMSYLLQDKEGQVTEAHSISAGLDYPGVGPEHSYLKDAGRAEYYSVTDQEAITALQRLSQLEGIIPALETAHAFAYLETLCPQLKNGERIVINCSGRGDKDVQTVAKY 407

HSP 2 Score: 31.187 bits (69), Expect = 3.783e-53
Identity = 12/19 (63.16%), Postives = 14/19 (73.68%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            GG PD+LL CVG GS  +G
Sbjct:  239 GGLPDILLACVGGGSNAMG 257

HSP 3 Score: 30.8018 bits (68), Expect = 3.783e-53
Identity = 12/20 (60.00%), Postives = 18/20 (90.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLF++FI++  VRLIG+
Sbjct:  254 NAMGLFYDFIDEPAVRLIGI 273
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB1_ANASP (Tryptophan synthase beta chain 1 OS=Anabaena sp. (strain PCC 7120) GN=trpB1 PE=3 SV=1)

HSP 1 Score: 180.644 bits (457), Expect = 3.111e-52
Identity = 83/132 (62.88%), Postives = 105/132 (79.55%), Query Frame = -1 
Query:  256 LEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTV 651
            +EAAG G+D+ KHAATL  G VGV HGAMSYLLQDE+GQ++  HS+  GL+YPGVGPE S+L+D  RAE+Y+ TD++A+  +Q+L RLEGI PALE SHA+A+LE LCP L    ++V+NCSG GDKDV TV
Sbjct:  272 VEAAGEGVDTDKHAATLTLGRVGVLHGAMSYLLQDEDGQVIEAHSISAGLDYPGVGPEHSYLKDIRRAEYYSVTDKQALDGFQQLSRLEGIIPALETSHAIAYLETLCPQLNGSPRIVINCSGRGDKDVQTV 403

HSP 2 Score: 35.039 bits (79), Expect = 3.111e-52
Identity = 14/20 (70.00%), Postives = 18/20 (90.00%), Query Frame = -3 
Query:  650 NALGLFHEFINDEDVRLIGV 709
            NA+GLFHEFI++  +RLIGV
Sbjct:  253 NAIGLFHEFIDEPSIRLIGV 272

HSP 3 Score: 31.9574 bits (71), Expect = 3.111e-52
Identity = 12/19 (63.16%), Postives = 14/19 (73.68%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            GG PD+LL CVG GS  +G
Sbjct:  238 GGLPDILLACVGGGSNAIG 256
[Close]
BLAST of CN185134 vs. ExPASy Swiss-Prot
Match: TRPB2_ANASP (Tryptophan synthase beta chain 2 OS=Anabaena sp. (strain PCC 7120) GN=trpB2 PE=3 SV=1)

HSP 1 Score: 189.889 bits (481), Expect = 1.594e-50
Identity = 89/149 (59.73%), Postives = 116/149 (77.85%), Query Frame = -1 
Query:  247 FMNS*MMKM*D*LELEAAGFGLDSGKHAATLAKGEVGVYHGAMSYLLQDEEGQILGTHSVGVGLEYPGVGPEISFLRDTGRAEFYTATDQEAVQAYQRLCRLEGIFPALEASHALAFLEKLCPTLPNGAKVVVNCSGGGDKDVDTVVNY 693
            F+N   +K+   + +EAAG G+++ KHAATL KG VGV HGAMSYLLQDE+GQ++  HS+  GL+YPGVGPE S+L+D GRAE+Y+ TD+EA+ A+QRL RLEGI PALE +HA+A+LE LCP L    ++V+NCSG GDKDV TV  +
Sbjct:  264 FVNESSIKL---IGVEAAGEGVNTEKHAATLTKGRVGVLHGAMSYLLQDEDGQVIEAHSISAGLDYPGVGPEHSYLKDVGRAEYYSVTDEEALAAFQRLSRLEGIIPALETAHAIAYLETLCPQLDGSPRIVINCSGRGDKDVQTVAKF 409

HSP 2 Score: 31.187 bits (69), Expect = 1.594e-50
Identity = 12/19 (63.16%), Postives = 14/19 (73.68%), Query Frame = -2 
Query:  699 GGKPDVLLLCVGSGSKCVG 755
            GG PD+LL CVG GS  +G
Sbjct:  241 GGLPDILLACVGGGSNAMG 259
The following BLAST results are available for this feature:
BLAST of CN185134 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 427
Match NameE-valueIdentityDescription
TRPB_CAMAC3.845e-6474.81Tryptophan synthase beta chain 2, chloroplastic OS... [more]
TRBP2_ARATH8.083e-6171.11Tryptophan synthase beta chain 2, chloroplastic OS... [more]
TRPB1_MAIZE7.179e-5967.41Tryptophan synthase beta chain 1 (Fragment) OS=Zea... [more]
TRPB2_MAIZE3.484e-5868.15Tryptophan synthase beta chain 2, chloroplastic (F... [more]
TRPB1_ARATH3.030e-5565.77Tryptophan synthase beta chain 1, chloroplastic OS... [more]
TRPB_ANAVT5.986e-5462.22Tryptophan synthase beta chain OS=Anabaena variabi... [more]
TRPB_THEEB1.014e-5365.19Tryptophan synthase beta chain OS=Thermosynechococ... [more]
TRPB_SYNY33.783e-5365.19Tryptophan synthase beta chain OS=Synechocystis sp... [more]
TRPB1_ANASP3.111e-5262.88Tryptophan synthase beta chain 1 OS=Anabaena sp. (... [more]
TRPB2_ANASP1.594e-5059.73Tryptophan synthase beta chain 2 OS=Anabaena sp. (... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0006N01_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0006N01, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN185134 ID=CN185134; Name=CN185134; organism=Citrus sinensis; type=EST; length=756bp
GCTTATAGATTGTACTTCCTATCAAAACAAATATCAACGAGTACAATAGT
TGCTTTGGCGATTGTGGCTCGGATTGGATCGAAAAACTTAGACATTAAAT
TAAAATATGCACAAAAGGGAGAAAAATAGGTCTTAAATTAATAGATTTCT
CGGGTTGCTGCTAAATTGACGGGAACCCTTAAATATTGTGGTCACCACTT
CTTCGATCCATTACAATTTCTACGTCGATAATTATTGATTGTATCTGTAA
TTGACGACCGTGTCGACGTCCTTATCCCCACCTCCACTACAGTTGACAAC
AACTTTTGCACCATTAGGTAAAGTAGGGCAAAGTTTCTCTAGAAATGCTA
ATGCATGAGATGCCTCAAGGGCCGGAAATATGCCTTCTAATCTGCACAGC
CGTTGATATGCTTGGACAGCTTCTTGGTCAGTCGCAGTGTAGAACTCCGC
ACGCCCAGTGTCCCTAAGAAAGCTTATCTCCGGGCCAACTCCTGGATACT
CTAGCCCTACACCGACAGAATGAGTCCCCAAGATTTGGCCTTCTTCATCT
TGCAACAAGTAGCTCATGGCTCCATGATAAACACCCACTTCCCCTTTAGC
CAGAGTTGCAGCGTGTTTGCCGCTGTCCAAGCCAAATCCAGCAGCCTCCA
ACTCCAATCAATCTCACATCTTCATCATTTATGAATTCATGAAACAAACC
CAACGCATTTTGATCCACTGCCCACACAAAGCAGCAATACATCAGGCTTG
CCTCCC
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