EG358200

Overview
NameEG358200
Unique NameEG358200
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length461
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig6850 contig Csv1_Contig6850:1..461. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of EG358200 vs. ExPASy Swiss-Prot
Match: EFTUB_NICSY (Elongation factor TuB, chloroplastic OS=Nicotiana sylvestris GN=TUFB PE=2 SV=1)

HSP 1 Score: 249.595 bits (636), Expect = 4.716e-66
Identity = 124/153 (81.05%), Postives = 131/153 (85.62%), Query Frame = 1 
Query:    1 CPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHILLAKQVGVPNMVVFLNKXXXXXXXXXXXXXXXXXXXXXSSYEFPGDDVPIISGSALLALEALMANPSIKRGENQWVDKIYELMDSVDSYIPIPQRQTDLPFLLAVEDVFSITGR 459
            CPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHILLAKQVGVPNMVVFLNK                     SSYEFPGD++PIISGSALLALEALMANPSIKRGENQWVDKIY+LMD+VD YIPIPQRQT+LPFL+A+EDVFSITGR
Sbjct:  158 CPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHILLAKQVGVPNMVVFLNKQDQVDDEELLELVELEVRELLSSYEFPGDEIPIISGSALLALEALMANPSIKRGENQWVDKIYQLMDNVDEYIPIPQRQTELPFLMAIEDVFSITGR 310
[Close]
BLAST of EG358200 vs. ExPASy Swiss-Prot
Match: EFTU_TOBAC (Elongation factor Tu, chloroplastic OS=Nicotiana tabacum GN=TUFA PE=3 SV=1)

HSP 1 Score: 249.21 bits (635), Expect = 6.159e-66
Identity = 125/153 (81.70%), Postives = 130/153 (84.97%), Query Frame = 1 
Query:    1 CPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHILLAKQVGVPNMVVFLNKXXXXXXXXXXXXXXXXXXXXXSSYEFPGDDVPIISGSALLALEALMANPSIKRGENQWVDKIYELMDSVDSYIPIPQRQTDLPFLLAVEDVFSITGR 459
            CPGHADYVKNMITGAAQMDGAILV SGADGPMPQTKEHILLAKQVGVPNMVVFLNK                     SSYEFPGDD+PIISGSALLALEALMANPSIKRGENQWVDKIYELMD+VDSYIPIP RQT+LPFL+A+EDVFSITGR
Sbjct:  151 CPGHADYVKNMITGAAQMDGAILVCSGADGPMPQTKEHILLAKQVGVPNMVVFLNKQDQVDDEELLQLVELEVRELLSSYEFPGDDIPIISGSALLALEALMANPSIKRGENQWVDKIYELMDAVDSYIPIPVRQTELPFLMAIEDVFSITGR 303
[Close]
BLAST of EG358200 vs. ExPASy Swiss-Prot
Match: EFTUA_NICSY (Elongation factor TuA, chloroplastic OS=Nicotiana sylvestris GN=TUFA PE=2 SV=2)

HSP 1 Score: 249.21 bits (635), Expect = 6.159e-66
Identity = 125/153 (81.70%), Postives = 130/153 (84.97%), Query Frame = 1 
Query:    1 CPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHILLAKQVGVPNMVVFLNKXXXXXXXXXXXXXXXXXXXXXSSYEFPGDDVPIISGSALLALEALMANPSIKRGENQWVDKIYELMDSVDSYIPIPQRQTDLPFLLAVEDVFSITGR 459
            CPGHADYVKNMITGAAQMDGAILV SGADGPMPQTKEHILLAKQVGVPNMVVFLNK                     SSYEFPGDD+PIISGSALLALEALMANPSIKRGENQWVDKIYELMD+VDSYIPIP RQT+LPFL+A+EDVFSITGR
Sbjct:  151 CPGHADYVKNMITGAAQMDGAILVCSGADGPMPQTKEHILLAKQVGVPNMVVFLNKQDQVDDEELLQLVELEVRELLSSYEFPGDDIPIISGSALLALEALMANPSIKRGENQWVDKIYELMDAVDSYIPIPVRQTELPFLMAIEDVFSITGR 303
[Close]
BLAST of EG358200 vs. ExPASy Swiss-Prot
Match: EFTU2_SOYBN (Elongation factor Tu, chloroplastic OS=Glycine max GN=TUFB1 PE=3 SV=1)

HSP 1 Score: 246.514 bits (628), Expect = 3.992e-65
Identity = 123/153 (80.39%), Postives = 130/153 (84.97%), Query Frame = 1 
Query:    1 CPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHILLAKQVGVPNMVVFLNKXXXXXXXXXXXXXXXXXXXXXSSYEFPGDDVPIISGSALLALEALMANPSIKRGENQWVDKIYELMDSVDSYIPIPQRQTDLPFLLAVEDVFSITGR 459
            CPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHILLAKQVGVPN+VVFLNK                     S YEFPGDDVPIISGSALL+LEALMANPSIKRGENQWVDKIYELM++VD YIPIPQRQT+LPFLLA+EDVF+ITGR
Sbjct:  152 CPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHILLAKQVGVPNIVVFLNKQDQVDDEELLQLVELEVRELLSKYEFPGDDVPIISGSALLSLEALMANPSIKRGENQWVDKIYELMEAVDDYIPIPQRQTELPFLLAIEDVFTITGR 304
[Close]
BLAST of EG358200 vs. ExPASy Swiss-Prot
Match: EFTU1_SOYBN (Elongation factor Tu, chloroplastic OS=Glycine max GN=TUFA PE=3 SV=1)

HSP 1 Score: 246.514 bits (628), Expect = 3.992e-65
Identity = 122/153 (79.74%), Postives = 130/153 (84.97%), Query Frame = 1 
Query:    1 CPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHILLAKQVGVPNMVVFLNKXXXXXXXXXXXXXXXXXXXXXSSYEFPGDDVPIISGSALLALEALMANPSIKRGENQWVDKIYELMDSVDSYIPIPQRQTDLPFLLAVEDVFSITGR 459
            CPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHI+LAKQVGVPNMVVFLNK                     SSYEFPGDD PI+SGSALLALEALMANP+IKRG+N+WVDKI++LMD VD+YIPIPQRQTDLPFLLAVEDVFSITGR
Sbjct:  152 CPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHIILAKQVGVPNMVVFLNKQDQVDDEELLQLVEIEVRDLLSSYEFPGDDTPIVSGSALLALEALMANPAIKRGDNEWVDKIFQLMDEVDNYIPIPQRQTDLPFLLAVEDVFSITGR 304
[Close]
BLAST of EG358200 vs. ExPASy Swiss-Prot
Match: EFTU_PEA (Elongation factor Tu, chloroplastic OS=Pisum sativum GN=tufA PE=2 SV=1)

HSP 1 Score: 241.121 bits (614), Expect = 1.677e-63
Identity = 119/153 (77.78%), Postives = 128/153 (83.66%), Query Frame = 1 
Query:    1 CPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHILLAKQVGVPNMVVFLNKXXXXXXXXXXXXXXXXXXXXXSSYEFPGDDVPIISGSALLALEALMANPSIKRGENQWVDKIYELMDSVDSYIPIPQRQTDLPFLLAVEDVFSITGR 459
            CPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHILLAKQVGVP++VVFLNK                     SSYEFPGDD+PI+SGSALLALEALMANP++KRG NQWVDKIY+LMD VD YIPIPQRQT+LPFLLA+EDVFSIT R
Sbjct:  161 CPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHILLAKQVGVPSVVVFLNKQDQVDDEELLELVELEVRELLSSYEFPGDDIPIVSGSALLALEALMANPTLKRGNNQWVDKIYQLMDEVDKYIPIPQRQTELPFLLAIEDVFSITXR 313
[Close]
BLAST of EG358200 vs. ExPASy Swiss-Prot
Match: EFTU_ARATH (Elongation factor Tu, chloroplastic OS=Arabidopsis thaliana GN=TUFA PE=1 SV=1)

HSP 1 Score: 236.498 bits (602), Expect = 4.131e-62
Identity = 118/153 (77.12%), Postives = 126/153 (82.35%), Query Frame = 1 
Query:    1 CPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHILLAKQVGVPNMVVFLNKXXXXXXXXXXXXXXXXXXXXXSSYEFPGDDVPIISGSALLALEALMANPSIKRGENQWVDKIYELMDSVDSYIPIPQRQTDLPFLLAVEDVFSITGR 459
            CPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHILLAKQVGVP+MVVFLNK                     SSYEF GDD+PIISGSALLA+E L  NP +KRG+N+WVDKIYELMD+VD YIPIPQRQT+LPFLLAVEDVFSITGR
Sbjct:  149 CPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHILLAKQVGVPDMVVFLNKEDQVDDAELLELVELEVRELLSSYEFNGDDIPIISGSALLAVETLTENPKVKRGDNKWVDKIYELMDAVDDYIPIPQRQTELPFLLAVEDVFSITGR 301
[Close]
BLAST of EG358200 vs. ExPASy Swiss-Prot
Match: EFTU_CHLRE (Elongation factor Tu, chloroplastic OS=Chlamydomonas reinhardtii GN=tufA PE=3 SV=1)

HSP 1 Score: 228.794 bits (582), Expect = 8.614e-60
Identity = 112/153 (73.20%), Postives = 123/153 (80.39%), Query Frame = 1 
Query:    1 CPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHILLAKQVGVPNMVVFLNKXXXXXXXXXXXXXXXXXXXXXSSYEFPGDDVPIISGSALLALEALMANPSIKRGENQWVDKIYELMDSVDSYIPIPQRQTDLPFLLAVEDVFSITGR 459
            CPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHILLAKQVGVPN+VVFLNK                       YEFPGD++P++ GSALLALEAL+ NP  +RGEN+WVDKIY+LMD+VDSYIP PQR+TD PFLLAVEDV SITGR
Sbjct:   82 CPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHILLAKQVGVPNVVVFLNKEDQVDDKELLELVELEVRETLDKYEFPGDEIPVVPGSALLALEALIENPKTQRGENKWVDKIYQLMDNVDSYIPTPQRETDKPFLLAVEDVLSITGR 234
[Close]
BLAST of EG358200 vs. ExPASy Swiss-Prot
Match: EFTU_SCEOB (Elongation factor Tu, chloroplastic OS=Scenedesmus obliquus GN=tufA PE=3 SV=1)

HSP 1 Score: 228.024 bits (580), Expect = 1.469e-59
Identity = 112/153 (73.20%), Postives = 122/153 (79.74%), Query Frame = 1 
Query:    1 CPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHILLAKQVGVPNMVVFLNKXXXXXXXXXXXXXXXXXXXXXSSYEFPGDDVPIISGSALLALEALMANPSIKRGENQWVDKIYELMDSVDSYIPIPQRQTDLPFLLAVEDVFSITGR 459
            CPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHILLAKQVGVPNMVVFLNK                       YEFPGD++PI+SGSALLALEAL+ NP I+RG+N+WVDKI++LMD VD YIP P R+TD PFLLAVEDV SITGR
Sbjct:   82 CPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHILLAKQVGVPNMVVFLNKEDQVDDAELLELVELEVRETLDKYEFPGDEIPIVSGSALLALEALVENPKIQRGDNKWVDKIFDLMDKVDEYIPTPDRETDKPFLLAVEDVLSITGR 234
[Close]
BLAST of EG358200 vs. ExPASy Swiss-Prot
Match: EFTU_NEPOL (Elongation factor Tu, chloroplastic OS=Nephroselmis olivacea GN=tufA PE=3 SV=1)

HSP 1 Score: 226.098 bits (575), Expect = 5.583e-59
Identity = 111/153 (72.55%), Postives = 123/153 (80.39%), Query Frame = 1 
Query:    1 CPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHILLAKQVGVPNMVVFLNKXXXXXXXXXXXXXXXXXXXXXSSYEFPGDDVPIISGSALLALEALMANPSIKRGENQWVDKIYELMDSVDSYIPIPQRQTDLPFLLAVEDVFSITGR 459
            CPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHILLAKQVGVPN+VVFLNK                     S+YEFPGD+VP++ GSALLALEA+  NPS+KRGEN+WVDKI+ LMD+VD YIP P+R TD  FL+AVEDVFSITGR
Sbjct:   83 CPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHILLAKQVGVPNIVVFLNKQDQVDDEELLELVELEVRETLSNYEFPGDEVPVVPGSALLALEAMTENPSLKRGENEWVDKIFALMDAVDQYIPTPKRDTDKSFLMAVEDVFSITGR 235
The following BLAST results are available for this feature:
BLAST of EG358200 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
EFTUB_NICSY4.716e-6681.05Elongation factor TuB, chloroplastic OS=Nicotiana ... [more]
EFTU_TOBAC6.159e-6681.70Elongation factor Tu, chloroplastic OS=Nicotiana t... [more]
EFTUA_NICSY6.159e-6681.70Elongation factor TuA, chloroplastic OS=Nicotiana ... [more]
EFTU2_SOYBN3.992e-6580.39Elongation factor Tu, chloroplastic OS=Glycine max... [more]
EFTU1_SOYBN3.992e-6579.74Elongation factor Tu, chloroplastic OS=Glycine max... [more]
EFTU_PEA1.677e-6377.78Elongation factor Tu, chloroplastic OS=Pisum sativ... [more]
EFTU_ARATH4.131e-6277.12Elongation factor Tu, chloroplastic OS=Arabidopsis... [more]
EFTU_CHLRE8.614e-6073.20Elongation factor Tu, chloroplastic OS=Chlamydomon... [more]
EFTU_SCEOB1.469e-5973.20Elongation factor Tu, chloroplastic OS=Scenedesmus... [more]
EFTU_NEPOL5.583e-5972.55Elongation factor Tu, chloroplastic OS=Nephroselmi... [more]

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Properties
Property NameValue
Genbank description10d5 Citrus sinensis flesh suppression subtractive hybridization (SSH) cDNA library Moro nucellare 58-8D-I cultivar Citrus sinensis cDNA clone Cl0010d5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EG358200 ID=EG358200; Name=EG358200; organism=Citrus sinensis; type=EST; length=461bp
TGCCCTGGCCACGCCGATTATGTTAAGAATATGATTACTGGCGCTGCCCA
AATGGACGGTGCGATTTTGGTGGTGTCCGGTGCTGATGGGCCAATGCCAC
AAACTAAAGAACATATTTTGTTGGCTAAGCAAGTGGGTGTCCCTAACATG
GTTGTGTTTTTGAATAAACAGGACCAGGTTGATGATGAAGAGCTGTTGCA
ACTTGTGGAATTGGAGGTTCGTGAGTTACTTTCTTCTTATGAATTTCCTG
GTGATGATGTGCCTATTATTTCTGGTTCTGCGCTCTTAGCTTTAGAGGCT
TTGATGGCTAATCCTAGTATTAAGAGAGGAGAGAATCAATGGGTTGATAA
GATTTATGAGCTTATGGATTCTGTTGATAGTTACATTCCTATCCCACAAA
GACAAACTGATTTGCCCTTTTTGTTAGCTGTTGAAGATGTGTTTTCAATT
ACTGGTCGTGG
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