CN181835

Overview
NameCN181835
Unique NameCN181835
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length675
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CN181835_ssr37 microsatellite CN181835_ssr37:37..69. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig9880 contig Csv1_Contig9880:65..739. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CN181835 vs. ExPASy Swiss-Prot
Match: PSA1B_ARATH (Proteasome subunit alpha type-1-B OS=Arabidopsis thaliana GN=PAF2 PE=1 SV=2)

HSP 1 Score: 330.487 bits (846), Expect = 4.948e-90
Identity = 161/173 (93.06%), Postives = 166/173 (95.95%), Query Frame = 3 
Query:  156 MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLGCVNKANSELSSHQKKIFKVDDHIGVAIAGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCTQRSWKRPYGVGLLVAGLDEKGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLE 674
            MFRNQYDTDVTTWSP GRLFQVEYAMEAVKQGSAAIGLRS++HVVL CVNKA SELSSHQ+KIFKVDDHIGVAIAGLTADGRVLSRYMRSE IN+S+TYESPLPVGRLVV LADKAQVCTQRSWKRPYGVGLLV GLDE GAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLE
Sbjct:    1 MFRNQYDTDVTTWSPTGRLFQVEYAMEAVKQGSAAIGLRSRSHVVLACVNKAQSELSSHQRKIFKVDDHIGVAIAGLTADGRVLSRYMRSESINHSFTYESPLPVGRLVVHLADKAQVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLE 173
[Close]
BLAST of CN181835 vs. ExPASy Swiss-Prot
Match: PSA1A_ARATH (Proteasome subunit alpha type-1-A OS=Arabidopsis thaliana GN=PAF1 PE=1 SV=3)

HSP 1 Score: 330.487 bits (846), Expect = 4.948e-90
Identity = 161/173 (93.06%), Postives = 166/173 (95.95%), Query Frame = 3 
Query:  156 MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLGCVNKANSELSSHQKKIFKVDDHIGVAIAGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCTQRSWKRPYGVGLLVAGLDEKGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLE 674
            MFRNQYDTDVTTWSP GRLFQVEYAMEAVKQGSAAIGLRS++HVVL CVNKA SELSSHQ+KIFKVDDHIGVAIAGLTADGRVLSRYMRSE IN+S+TYESPLPVGRLVV LADKAQVCTQRSWKRPYGVGLLV GLDE GAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLE
Sbjct:    1 MFRNQYDTDVTTWSPTGRLFQVEYAMEAVKQGSAAIGLRSRSHVVLACVNKAQSELSSHQRKIFKVDDHIGVAIAGLTADGRVLSRYMRSESINHSFTYESPLPVGRLVVHLADKAQVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLE 173
[Close]
BLAST of CN181835 vs. ExPASy Swiss-Prot
Match: PSA1_ORYSJ (Proteasome subunit alpha type-1 OS=Oryza sativa subsp. japonica GN=PAF1 PE=2 SV=1)

HSP 1 Score: 308.145 bits (788), Expect = 2.630e-83
Identity = 145/173 (83.82%), Postives = 161/173 (93.06%), Query Frame = 3 
Query:  156 MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLGCVNKANSELSSHQKKIFKVDDHIGVAIAGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCTQRSWKRPYGVGLLVAGLDEKGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLE 674
            MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSA +GLRS+TH VL   NKA SELSSHQ+K+F+V DH GVA+AGLTADGRVLSR++RSECIN+++ Y++PLPV RL ++LADKAQVCTQRSWKRPYGVGLLVAGLDE GAHLYYNCPSGNYFEYQAFAIGSRSQAAKT+LE
Sbjct:    1 MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSACVGLRSRTHAVLAAANKAASELSSHQRKVFRVADHAGVALAGLTADGRVLSRFLRSECINHAFVYDAPLPVSRLALRLADKAQVCTQRSWKRPYGVGLLVAGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTFLE 173
[Close]
BLAST of CN181835 vs. ExPASy Swiss-Prot
Match: PSA1_DICDI (Proteasome subunit alpha type-1 OS=Dictyostelium discoideum GN=psmA1 PE=3 SV=1)

HSP 1 Score: 238.039 bits (606), Expect = 3.342e-62
Identity = 111/173 (64.16%), Postives = 144/173 (83.24%), Query Frame = 3 
Query:  156 MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLGCVNKANSELSSHQKKIFKVDDHIGVAIAGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCTQRSWKRPYGVGLLVAGLDEKGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLE 674
            M+RNQYD+D+TT+SP+GR+ Q+EYAMEAVKQG AA+ L+SK   VL  + +++SEL S+QKKIF VDDH+G+AI+GLTAD R+L  YMR+EC NY+Y YES + V RLV ++ADK QV TQR  +RPYGVGLLVAG D+ GAH+Y  CPSGN+++Y++ +IGSRSQ+AKTYLE
Sbjct:    1 MYRNQYDSDITTFSPSGRIHQIEYAMEAVKQGGAAVALKSKQFAVLVSLKRSSSELGSYQKKIFVVDDHVGIAISGLTADARMLCNYMRNECSNYNYVYESQMRVERLVTKVADKHQVHTQRYGRRPYGVGLLVAGYDQLGAHIYQTCPSGNFYDYKSISIGSRSQSAKTYLE 173
[Close]
BLAST of CN181835 vs. ExPASy Swiss-Prot
Match: PSA1_PONAB (Proteasome subunit alpha type-1 OS=Pongo abelii GN=PSMA1 PE=2 SV=1)

HSP 1 Score: 230.335 bits (586), Expect = 6.969e-60
Identity = 105/173 (60.69%), Postives = 136/173 (78.61%), Query Frame = 3 
Query:  156 MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLGCVNKANSELSSHQKKIFKVDDHIGVAIAGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCTQRSWKRPYGVGLLVAGLDEKGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLE 674
            MFRNQYD DVT WSP GR+ Q+EYAMEAVKQGSA +GL+SKTH VL  + +A SEL++HQKKI  VD+HIG++IAGLTAD R+L  +MR EC++  + ++ PLPV RLV  +  K Q+ TQR  +RPYGVGLL+AG D+ G H++  CPS NYF+ +A +IG+RSQ+A+TYLE
Sbjct:    1 MFRNQYDNDVTVWSPQGRIHQIEYAMEAVKQGSATVGLKSKTHAVLVALKRAQSELAAHQKKILHVDNHIGISIAGLTADARLLCNFMRQECLDSRFVFDRPLPVSRLVSLIGSKTQIPTQRYGRRPYGVGLLIAGYDDMGPHIFQTCPSANYFDCRAMSIGARSQSARTYLE 173
[Close]
BLAST of CN181835 vs. ExPASy Swiss-Prot
Match: PSA1_MOUSE (Proteasome subunit alpha type-1 OS=Mus musculus GN=Psma1 PE=1 SV=1)

HSP 1 Score: 230.335 bits (586), Expect = 6.969e-60
Identity = 105/173 (60.69%), Postives = 136/173 (78.61%), Query Frame = 3 
Query:  156 MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLGCVNKANSELSSHQKKIFKVDDHIGVAIAGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCTQRSWKRPYGVGLLVAGLDEKGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLE 674
            MFRNQYD DVT WSP GR+ Q+EYAMEAVKQGSA +GL+SKTH VL  + +A SEL++HQKKI  VD+HIG++IAGLTAD R+L  +MR EC++  + ++ PLPV RLV  +  K Q+ TQR  +RPYGVGLL+AG D+ G H++  CPS NYF+ +A +IG+RSQ+A+TYLE
Sbjct:    1 MFRNQYDNDVTVWSPQGRIHQIEYAMEAVKQGSATVGLKSKTHAVLVALKRAQSELAAHQKKILHVDNHIGISIAGLTADARLLCNFMRQECLDSRFVFDRPLPVSRLVSLIGSKTQIPTQRYGRRPYGVGLLIAGYDDMGPHIFQTCPSANYFDCRAMSIGARSQSARTYLE 173
[Close]
BLAST of CN181835 vs. ExPASy Swiss-Prot
Match: PSA1_HUMAN (Proteasome subunit alpha type-1 OS=Homo sapiens GN=PSMA1 PE=1 SV=1)

HSP 1 Score: 230.335 bits (586), Expect = 6.969e-60
Identity = 105/173 (60.69%), Postives = 136/173 (78.61%), Query Frame = 3 
Query:  156 MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLGCVNKANSELSSHQKKIFKVDDHIGVAIAGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCTQRSWKRPYGVGLLVAGLDEKGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLE 674
            MFRNQYD DVT WSP GR+ Q+EYAMEAVKQGSA +GL+SKTH VL  + +A SEL++HQKKI  VD+HIG++IAGLTAD R+L  +MR EC++  + ++ PLPV RLV  +  K Q+ TQR  +RPYGVGLL+AG D+ G H++  CPS NYF+ +A +IG+RSQ+A+TYLE
Sbjct:    1 MFRNQYDNDVTVWSPQGRIHQIEYAMEAVKQGSATVGLKSKTHAVLVALKRAQSELAAHQKKILHVDNHIGISIAGLTADARLLCNFMRQECLDSRFVFDRPLPVSRLVSLIGSKTQIPTQRYGRRPYGVGLLIAGYDDMGPHIFQTCPSANYFDCRAMSIGARSQSARTYLE 173
[Close]
BLAST of CN181835 vs. ExPASy Swiss-Prot
Match: PSA1_BOVIN (Proteasome subunit alpha type-1 OS=Bos taurus GN=PSMA1 PE=1 SV=1)

HSP 1 Score: 230.335 bits (586), Expect = 6.969e-60
Identity = 105/173 (60.69%), Postives = 136/173 (78.61%), Query Frame = 3 
Query:  156 MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLGCVNKANSELSSHQKKIFKVDDHIGVAIAGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCTQRSWKRPYGVGLLVAGLDEKGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLE 674
            MFRNQYD DVT WSP GR+ Q+EYAMEAVKQGSA +GL+SKTH VL  + +A SEL++HQKKI  VD+HIG++IAGLTAD R+L  +MR EC++  + ++ PLPV RLV  +  K Q+ TQR  +RPYGVGLL+AG D+ G H++  CPS NYF+ +A +IG+RSQ+A+TYLE
Sbjct:    1 MFRNQYDNDVTVWSPQGRIHQIEYAMEAVKQGSATVGLKSKTHAVLVALKRAQSELAAHQKKILHVDNHIGISIAGLTADARLLCNFMRQECLDSRFVFDRPLPVSRLVSLIGSKTQIPTQRYGRRPYGVGLLIAGYDDMGPHIFQTCPSANYFDCRAMSIGARSQSARTYLE 173
[Close]
BLAST of CN181835 vs. ExPASy Swiss-Prot
Match: PSA1_RAT (Proteasome subunit alpha type-1 OS=Rattus norvegicus GN=Psma1 PE=1 SV=2)

HSP 1 Score: 229.95 bits (585), Expect = 9.102e-60
Identity = 105/173 (60.69%), Postives = 136/173 (78.61%), Query Frame = 3 
Query:  156 MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLGCVNKANSELSSHQKKIFKVDDHIGVAIAGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCTQRSWKRPYGVGLLVAGLDEKGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLE 674
            MFRNQYD DVT WSP GR+ Q+EYAMEAVKQGSA +GL+SKTH VL  + +A SEL++HQKKI  VD+HIG++IAGLTAD R+L  +MR EC++  + ++ PLPV RLV  +  K Q+ TQR  +RPYGVGLL+AG D+ G H++  CPS NYF+ +A +IG+RSQ+A+TYLE
Sbjct:    1 MFRNQYDNDVTVWSPQGRIHQIEYAMEAVKQGSATVGLKSKTHAVLVALKRAQSELAAHQKKILHVDNHIGISIAGLTADARLLCNFMRQECLDSRFVFDRPLPVSRLVSLIGSKTQIPTQRYGRRPYGVGLLIAGYDDMGPHVFQTCPSANYFDCRAMSIGARSQSARTYLE 173
[Close]
BLAST of CN181835 vs. ExPASy Swiss-Prot
Match: PSA1_MACFA (Proteasome subunit alpha type-1 OS=Macaca fascicularis GN=PSMA1 PE=2 SV=1)

HSP 1 Score: 228.794 bits (582), Expect = 2.028e-59
Identity = 104/173 (60.12%), Postives = 136/173 (78.61%), Query Frame = 3 
Query:  156 MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLGCVNKANSELSSHQKKIFKVDDHIGVAIAGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCTQRSWKRPYGVGLLVAGLDEKGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLE 674
            MFRNQYD DVT WSP GR+ Q+EYAMEAVKQGSA +GL+SKTH VL  + +A SEL++H+KKI  VD+HIG++IAGLTAD R+L  +MR EC++  + ++ PLPV RLV  +  K Q+ TQR  +RPYGVGLL+AG D+ G H++  CPS NYF+ +A +IG+RSQ+A+TYLE
Sbjct:    1 MFRNQYDNDVTVWSPQGRIHQIEYAMEAVKQGSATVGLKSKTHAVLVALKRAQSELAAHRKKILHVDNHIGISIAGLTADARLLCNFMRQECLDSRFVFDRPLPVSRLVSLIGSKTQIPTQRYGRRPYGVGLLIAGYDDMGPHIFQTCPSANYFDCRAMSIGARSQSARTYLE 173
The following BLAST results are available for this feature:
BLAST of CN181835 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 174
Match NameE-valueIdentityDescription
PSA1B_ARATH4.948e-9093.06Proteasome subunit alpha type-1-B OS=Arabidopsis t... [more]
PSA1A_ARATH4.948e-9093.06Proteasome subunit alpha type-1-A OS=Arabidopsis t... [more]
PSA1_ORYSJ2.630e-8383.82Proteasome subunit alpha type-1 OS=Oryza sativa su... [more]
PSA1_DICDI3.342e-6264.16Proteasome subunit alpha type-1 OS=Dictyostelium d... [more]
PSA1_PONAB6.969e-6060.69Proteasome subunit alpha type-1 OS=Pongo abelii GN... [more]
PSA1_MOUSE6.969e-6060.69Proteasome subunit alpha type-1 OS=Mus musculus GN... [more]
PSA1_HUMAN6.969e-6060.69Proteasome subunit alpha type-1 OS=Homo sapiens GN... [more]
PSA1_BOVIN6.969e-6060.69Proteasome subunit alpha type-1 OS=Bos taurus GN=P... [more]
PSA1_RAT9.102e-6060.69Proteasome subunit alpha type-1 OS=Rattus norvegic... [more]
PSA1_MACFA2.028e-5960.12Proteasome subunit alpha type-1 OS=Macaca fascicul... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0001I21_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0001I21, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN181835 ID=CN181835; Name=CN181835; organism=Citrus sinensis; type=EST; length=675bp
GAGGCTTGGCTTGAAGAAAAAGAAAACACTTTCTACTATTATTATTATTA
TTATTATTATTATTATTATTAAGACGGCTTTTGGTTGCAGTAGATTTACT
TGCAGCGTTTGTTTCATCGCGAAATTAATACGGCACGAAAGATTAAACCC
TAAAAATGTTTAGGAACCAATACGACACCGACGTGACAACATGGAGCCCC
GCGGGCCGATTATTTCAAGTGGAGTACGCGATGGAGGCCGTCAAGCAAGG
CTCCGCCGCAATTGGGCTCCGATCTAAGACTCACGTGGTCTTGGGCTGCG
TTAACAAGGCCAACTCCGAGCTCTCGTCTCACCAGAAGAAGATCTTCAAG
GTCGACGACCACATCGGCGTCGCTATCGCCGGTCTCACCGCCGACGGCCG
TGTTCTGTCTCGGTACATGCGATCCGAGTGTATTAACTACAGTTACACTT
ACGAGTCGCCGCTTCCTGTCGGCCGCCTTGTTGTCCAGCTCGCTGACAAG
GCTCAGGTCTGTACCCAGCGCTCATGGAAACGGCCATATGGTGTTGGCCT
GCTGGTTGCTGGCTTGGATGAAAAAGGAGCCCACCTTTATTATAACTGCC
CAAGTGGGAACTATTTTGAGTATCAAGCATTTGCCATTGGGTCCCGATCT
CAAGCTGCAAAGACATACTTGGAAC
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