CN188720

Overview
NameCN188720
Unique NameCN188720
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length725
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig12127 contig Csv1_Contig12127:429..1153. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CN188720 vs. ExPASy Swiss-Prot
Match: ARD3_ARATH (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 3 OS=Arabidopsis thaliana GN=ARD3 PE=2 SV=1)

HSP 1 Score: 358.221 bits (918), Expect = 2.525e-98
Identity = 160/187 (85.56%), Postives = 176/187 (94.12%), Query Frame = 2 
Query:   74 QDPREEVIQAWYMDDSDEDQRLPHHKDPKEFVSLDQLSELGVLSWRLDADNYETDEELKKIREDRGYSYMDFCEVCPEKLPNYEEKIKNFFEEHLHTDEEIRYCVAGSGYFDVRDHNEKWIRIWVKKGGMIVLPAGCYHRFTLDTDNYIKAMRLFVGDPVWTPFNRPHDHLPARKGYVQNFLQKEAG 634
            +D REEVIQAWYMDDS+EDQRLPHHKDPKEF+SLD+L+ELGVLSWRLDADNYETDE+LKKIRE RGYSYMDFCEVCPEKLPNYE K+K+FFEEHLHTDEEIRYCVAGSGYFDVRD NE WIR+WVKKGGMIVLPAG YHRFT+D+DNYIKAMRLFVG+PVWTP+NRPHDHLPARK Y+ NF++   G
Sbjct:    6 KDGREEVIQAWYMDDSEEDQRLPHHKDPKEFLSLDKLAELGVLSWRLDADNYETDEDLKKIRESRGYSYMDFCEVCPEKLPNYEVKVKSFFEEHLHTDEEIRYCVAGSGYFDVRDRNEAWIRVWVKKGGMIVLPAGIYHRFTVDSDNYIKAMRLFVGEPVWTPYNRPHDHLPARKEYIDNFVKVNEG 192
[Close]
BLAST of CN188720 vs. ExPASy Swiss-Prot
Match: ARD2_ARATH (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 2 OS=Arabidopsis thaliana GN=ARD2 PE=2 SV=1)

HSP 1 Score: 353.214 bits (905), Expect = 8.122e-97
Identity = 160/184 (86.96%), Postives = 174/184 (94.57%), Query Frame = 2 
Query:   68 APQDPREEVIQAWYMDDSDEDQRLPHHKDPKEFVSLDQLSELGVLSWRLDADNYETDEELKKIREDRGYSYMDFCEVCPEKLPNYEEKIKNFFEEHLHTDEEIRYCVAGSGYFDVRDHNEKWIRIWVKKGGMIVLPAGCYHRFTLDTDNYIKAMRLFVGDPVWTPFNRPHDHLPARKGYVQNFL 619
            A +D REEVIQAWYMDDS+EDQRLPHHKDPKEFVSLD+L+ELGVLSWRLDADNYETDE+LKKIRE RGYSYMDFCEVCPEKLPNYE K+K+FFEEHLHTDEEIRYCVAG+GYFDVRD NE WIR+ VKKGGMIVLPAG YHRFT+D+DNYIKAMRLFVG+PVWTP+NRPHDHLPARK YV NF+
Sbjct:    4 AVKDGREEVIQAWYMDDSEEDQRLPHHKDPKEFVSLDKLAELGVLSWRLDADNYETDEDLKKIRESRGYSYMDFCEVCPEKLPNYEVKVKSFFEEHLHTDEEIRYCVAGTGYFDVRDRNEAWIRVLVKKGGMIVLPAGIYHRFTVDSDNYIKAMRLFVGEPVWTPYNRPHDHLPARKEYVDNFM 187
[Close]
BLAST of CN188720 vs. ExPASy Swiss-Prot
Match: ARD1_ARATH (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 1 OS=Arabidopsis thaliana GN=ARD1 PE=2 SV=1)

HSP 1 Score: 338.191 bits (866), Expect = 2.704e-92
Identity = 154/197 (78.17%), Postives = 175/197 (88.83%), Query Frame = 2 
Query:   68 APQDPREEVIQAWYMDDSDEDQRLPHHKDPKEFVSLDQLSELGVLSWRLDADNYETDEELKKIREDRGYSYMDFCEVCPEKLPNYEEKIKNFFEEHLHTDEEIRYCVAGSGYFDVRDHNEKWIRIWVKKGGMIVLPAGCYHRFTLDTDNYIKAMRLFVGDPVWTPFNRPHDHLPARKGYVQNFLQKEAGDSPINAAA 658
            A +D  EEVIQAWY+D+ +EDQ+LPHHKDPKEFVSLD+L+ELGVL WRLDADNYETDEELK+IRE RGYSYMD CEVCPEKLPNYEEK+K FFEEHLH DEEIRYC+AGSGYFDVRD N+ WIR+WVKKGG+IV PAG YHRFT+D+DNY+KAMRLFVG PVWT +NRPHDHLPARK Y++ FL K  GD  I+A+A
Sbjct:    4 AAKDQTEEVIQAWYLDNKEEDQKLPHHKDPKEFVSLDKLAELGVLCWRLDADNYETDEELKRIRESRGYSYMDLCEVCPEKLPNYEEKVKMFFEEHLHIDEEIRYCLAGSGYFDVRDLNDIWIRVWVKKGGLIVFPAGIYHRFTVDSDNYMKAMRLFVGGPVWTAYNRPHDHLPARKAYMKKFL-KVIGDRNIDASA 199
[Close]
BLAST of CN188720 vs. ExPASy Swiss-Prot
Match: ARD2_ORYSJ (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 2 OS=Oryza sativa subsp. japonica GN=ARD2 PE=2 SV=1)

HSP 1 Score: 329.717 bits (844), Expect = 9.616e-90
Identity = 145/184 (78.80%), Postives = 168/184 (91.30%), Query Frame = 2 
Query:   74 QDPREEVIQAWYMDDSDEDQRLPHHKDPKEFVSLDQLSELGVLSWRLDADNYETDEELKKIREDRGYSYMDFCEVCPEKLPNYEEKIKNFFEEHLHTDEEIRYCVAGSGYFDVRDHNEKWIRIWVKKGGMIVLPAGCYHRFTLDTDNYIKAMRLFVGDPVWTPFNRPHDHLPARKGYVQNFLQK 625
            QD +EEVI+AWYMDDS+EDQRLPHH++PKEF+ L +LSELG+LSWRL+AD++E DE LKKIRE RGYSYMD C+VCPEKLPNYE K+KNFFEEHLHTDEEIRYC+ GSGYFDVRD N++WIR+ VKKGGMIVLPAG YHRFTLD+DNYIKAMRLFVG+PVWTP+NRPHDHLPARK YV+  + +
Sbjct:    6 QDGKEEVIEAWYMDDSEEDQRLPHHREPKEFIPLSKLSELGILSWRLNADDWENDENLKKIREARGYSYMDICDVCPEKLPNYEAKLKNFFEEHLHTDEEIRYCLEGSGYFDVRDQNDQWIRVAVKKGGMIVLPAGMYHRFTLDSDNYIKAMRLFVGEPVWTPYNRPHDHLPARKEYVEKIINR 189
[Close]
BLAST of CN188720 vs. ExPASy Swiss-Prot
Match: ARD2_ORYSI (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 2 OS=Oryza sativa subsp. indica GN=ARD2 PE=2 SV=2)

HSP 1 Score: 329.717 bits (844), Expect = 9.616e-90
Identity = 145/184 (78.80%), Postives = 168/184 (91.30%), Query Frame = 2 
Query:   74 QDPREEVIQAWYMDDSDEDQRLPHHKDPKEFVSLDQLSELGVLSWRLDADNYETDEELKKIREDRGYSYMDFCEVCPEKLPNYEEKIKNFFEEHLHTDEEIRYCVAGSGYFDVRDHNEKWIRIWVKKGGMIVLPAGCYHRFTLDTDNYIKAMRLFVGDPVWTPFNRPHDHLPARKGYVQNFLQK 625
            QD +EEVI+AWYMDDS+EDQRLPHH++PKEF+ L +LSELG+LSWRL+AD++E DE LKKIRE RGYSYMD C+VCPEKLPNYE K+KNFFEEHLHTDEEIRYC+ GSGYFDVRD N++WIR+ VKKGGMIVLPAG YHRFTLD+DNYIKAMRLFVG+PVWTP+NRPHDHLPARK YV+  + +
Sbjct:    6 QDGKEEVIEAWYMDDSEEDQRLPHHREPKEFIPLSKLSELGILSWRLNADDWENDENLKKIREARGYSYMDICDVCPEKLPNYEAKLKNFFEEHLHTDEEIRYCLEGSGYFDVRDQNDQWIRVAVKKGGMIVLPAGMYHRFTLDSDNYIKAMRLFVGEPVWTPYNRPHDHLPARKEYVEKIINR 189
[Close]
BLAST of CN188720 vs. ExPASy Swiss-Prot
Match: ARD1_ORYSJ (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 1 OS=Oryza sativa subsp. japonica GN=ARD1 PE=2 SV=1)

HSP 1 Score: 320.857 bits (821), Expect = 4.467e-87
Identity = 142/191 (74.35%), Postives = 168/191 (87.96%), Query Frame = 2 
Query:   74 QDPREEVIQAWYMDDSDEDQRLPHHKDPKEFVSLDQLSELGVLSWRLDADNYETDEELKKIREDRGYSYMDFCEVCPEKLPNYEEKIKNFFEEHLHTDEEIRYCVAGSGYFDVRDHNEKWIRIWVKKGGMIVLPAGCYHRFTLDTDNYIKAMRLFVGDPVWTPFNRPHDHLPARKGYVQNFLQKEAGDSPI 646
            QD + EVI+AWYMDDS+EDQRLPHH++PKEF+ +D+L+ELGV+SWRL+ DN+E  E LK+IRE RGYSY+D C+VCPEKLPNYE KIK+FFEEHLHTDEEIRYC+ GSGYFDVRD N++WIRI +KKGGMIVLPAG YHRFTLDTDNYIKAMRLFVGDPVWTP+NRPHDHLPARK ++   L+ E  +  +
Sbjct:    6 QDGKTEVIEAWYMDDSEEDQRLPHHREPKEFIHVDKLTELGVISWRLNPDNWENCENLKRIREARGYSYVDICDVCPEKLPNYETKIKSFFEEHLHTDEEIRYCLEGSGYFDVRDQNDQWIRIALKKGGMIVLPAGMYHRFTLDTDNYIKAMRLFVGDPVWTPYNRPHDHLPARKEFLAKLLKSEGENQAV 196
[Close]
BLAST of CN188720 vs. ExPASy Swiss-Prot
Match: ARD1_ORYSI (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 1 OS=Oryza sativa subsp. indica GN=ARD1 PE=1 SV=2)

HSP 1 Score: 320.857 bits (821), Expect = 4.467e-87
Identity = 142/191 (74.35%), Postives = 168/191 (87.96%), Query Frame = 2 
Query:   74 QDPREEVIQAWYMDDSDEDQRLPHHKDPKEFVSLDQLSELGVLSWRLDADNYETDEELKKIREDRGYSYMDFCEVCPEKLPNYEEKIKNFFEEHLHTDEEIRYCVAGSGYFDVRDHNEKWIRIWVKKGGMIVLPAGCYHRFTLDTDNYIKAMRLFVGDPVWTPFNRPHDHLPARKGYVQNFLQKEAGDSPI 646
            QD + EVI+AWYMDDS+EDQRLPHH++PKEF+ +D+L+ELGV+SWRL+ DN+E  E LK+IRE RGYSY+D C+VCPEKLPNYE KIK+FFEEHLHTDEEIRYC+ GSGYFDVRD N++WIRI +KKGGMIVLPAG YHRFTLDTDNYIKAMRLFVGDPVWTP+NRPHDHLPARK ++   L+ E  +  +
Sbjct:    6 QDGKTEVIEAWYMDDSEEDQRLPHHREPKEFIHVDKLTELGVISWRLNPDNWENCENLKRIREARGYSYVDICDVCPEKLPNYETKIKSFFEEHLHTDEEIRYCLEGSGYFDVRDQNDQWIRIALKKGGMIVLPAGMYHRFTLDTDNYIKAMRLFVGDPVWTPYNRPHDHLPARKEFLAKLLKSEGENQAV 196
[Close]
BLAST of CN188720 vs. ExPASy Swiss-Prot
Match: ARD4_ARATH (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 4 OS=Arabidopsis thaliana GN=ARD4 PE=2 SV=1)

HSP 1 Score: 280.796 bits (717), Expect = 5.123e-75
Identity = 121/177 (68.36%), Postives = 147/177 (83.05%), Query Frame = 2 
Query:   95 IQAWYMDDSDEDQRLPHHKDPKEFVSLDQLSELGVLSWRLDADNYETDEELKKIREDRGYSYMDFCEVCPEKLPNYEEKIKNFFEEHLHTDEEIRYCVAGSGYFDVRDHNEKWIRIWVKKGGMIVLPAGCYHRFTLDTDNYIKAMRLFVGDPVWTPFNRPHDHLPARKGYVQNFLQK 625
            ++AW+MDDS+EDQRLPHH++PKE VSLD L+ELGVL W+L+ +NYE D EL KIREDRGY YMD  ++CPEK+ NYEEK+KNFF EH+H DEEIRYC+AGSGYFDVRD +++WIRIW++ G +IVLPAG YHRFTLD  NYIK MRLFVG+PVWTP+NRP +  P RK Y+     K
Sbjct:    3 LEAWFMDDSNEDQRLPHHRNPKELVSLDYLAELGVLYWKLNPENYENDSELSKIREDRGYDYMDLLDLCPEKVSNYEEKLKNFFTEHIHKDEEIRYCLAGSGYFDVRDKDDRWIRIWMQPGDLIVLPAGIYHRFTLDASNYIKLMRLFVGEPVWTPYNRPQEEHPVRKKYIHGLTYK 179
[Close]
BLAST of CN188720 vs. ExPASy Swiss-Prot
Match: ARD3_ORYSJ (Putative 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 3 OS=Oryza sativa subsp. japonica GN=ARD3 PE=3 SV=3)

HSP 1 Score: 259.996 bits (663), Expect = 9.357e-69
Identity = 117/178 (65.73%), Postives = 143/178 (80.34%), Query Frame = 2 
Query:   86 EEVIQAWYMDDSD---EDQRLPHHKDPKEFVSLDQLSELGVLSWRLDADNYETDEELKKIREDRGYSYMDFCEVCPEKLPNYEEKIKNFFEEHLHTDEEIRYCVAGSGYFDVRDHNEKWIRIWVKKGGMIVLPAGCYHRFTLDTDNYIKAMRLFVGDPVWTPFNRPHDHLPARKGYVQ 610
            EEV+QAWYMDD D   EDQRLPH + P + + L +L +LG+++ RLDADN+E DE LK +RE RGY +MD  E+ PEK+PNYE  IK FFEEHLHTDEE+RYC+ GSGYFDVRD N+KW+R+ V+KG +IV+PAG YHRFTLDT+NYIK MRLF G P WT +NRPHDHLP RK Y++
Sbjct:    9 EEVVQAWYMDDDDNAEEDQRLPHRRQPDDLLPLAKLLDLGLVAMRLDADNHEHDENLKIMREQRGYLHMDIVELTPEKMPNYEVMIKRFFEEHLHTDEEVRYCLDGSGYFDVRDENDKWVRVSVRKGALIVVPAGIYHRFTLDTNNYIKTMRLFSGGPDWTAYNRPHDHLPERKKYLE 186
[Close]
BLAST of CN188720 vs. ExPASy Swiss-Prot
Match: MTND_BOVIN (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase OS=Bos taurus GN=ADI1 PE=2 SV=1)

HSP 1 Score: 250.366 bits (638), Expect = 7.414e-66
Identity = 115/180 (63.89%), Postives = 141/180 (78.33%), Query Frame = 2 
Query:   92 VIQAWYMDDSDEDQRLPHHKDPKEFVSLDQLSELGVLSWRLDADNYETDEELKKIREDRGYSYMDFCEVCPEKLPNYEEKIKNFFEEHLHTDEEIRYCVAGSGYFDVRDHNEKWIRIWVKKGGMIVLPAGCYHRFTLDTDNYIKAMRLFVGDPVWTPFNRPHDHLPARKGYVQNFLQKEA 631
            +++AWY+DD+ +D RLPH  +P   V L+QL  LGVL W+LDAD YE D EL+KIR++R YS++D   +  +KLPNYEEKIK FFEEHLH DEEIRY + GSGYFDVRD  ++WIRI ++KG MI LPAG YHRFTLD  NY+KAMRLFVGDPVWTP+NRP DHL AR  Y++ FL + A
Sbjct:    1 MVEAWYIDDAADDPRLPHRAEPARPVGLEQLRRLGVLYWKLDADKYENDPELEKIRKERNYSWVDIITISKDKLPNYEEKIKMFFEEHLHLDEEIRYILDGSGYFDVRDQEDRWIRISMEKGDMITLPAGIYHRFTLDEKNYVKAMRLFVGDPVWTPYNRPSDHLEARTQYLE-FLAQSA 179
The following BLAST results are available for this feature:
BLAST of CN188720 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 38
Match NameE-valueIdentityDescription
ARD3_ARATH2.525e-9885.561,2-dihydroxy-3-keto-5-methylthiopentene dioxygena... [more]
ARD2_ARATH8.122e-9786.961,2-dihydroxy-3-keto-5-methylthiopentene dioxygena... [more]
ARD1_ARATH2.704e-9278.171,2-dihydroxy-3-keto-5-methylthiopentene dioxygena... [more]
ARD2_ORYSJ9.616e-9078.801,2-dihydroxy-3-keto-5-methylthiopentene dioxygena... [more]
ARD2_ORYSI9.616e-9078.801,2-dihydroxy-3-keto-5-methylthiopentene dioxygena... [more]
ARD1_ORYSJ4.467e-8774.351,2-dihydroxy-3-keto-5-methylthiopentene dioxygena... [more]
ARD1_ORYSI4.467e-8774.351,2-dihydroxy-3-keto-5-methylthiopentene dioxygena... [more]
ARD4_ARATH5.123e-7568.361,2-dihydroxy-3-keto-5-methylthiopentene dioxygena... [more]
ARD3_ORYSJ9.357e-6965.73Putative 1,2-dihydroxy-3-keto-5-methylthiopentene ... [more]
MTND_BOVIN7.414e-6663.891,2-dihydroxy-3-keto-5-methylthiopentene dioxygena... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0006K08_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0006K08, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN188720 ID=CN188720; Name=CN188720; organism=Citrus sinensis; type=EST; length=725bp
AGTCTCAACCTCATTTCTATTGCGTTGCCACCAAGCGTTCGTTCAAAGAA
TTTCAGAAACCATGGTTGCCCCTCAGGATCCTAGAGAGGAAGTTATTCAG
GCATGGTACATGGATGATAGTGATGAAGATCAAAGACTCCCCCATCACAA
GGATCCAAAGGAATTTGTGTCCCTGGACCAGCTTTCTGAACTTGGAGTAC
TCAGCTGGCGATTAGATGCTGATAACTATGAAACTGATGAGGAACTGAAG
AAGATTCGTGAAGATCGCGGATATTCTTACATGGACTTTTGTGAGGTTTG
CCCAGAAAAACTGCCAAATTATGAAGAGAAGATCAAAAACTTTTTCGAAG
AACATCTTCATACAGATGAAGAGATCCGCTACTGTGTGGCAGGAAGTGGT
TACTTTGATGTCAGGGACCATAATGAAAAATGGATTCGCATATGGGTAAA
GAAAGGAGGAATGATTGTTTTACCTGCTGGATGTTACCATCGCTTTACTT
TGGATACAGACAACTATATCAAGGCTATGCGTCTGTTTGTAGGTGATCCC
GTTTGGACTCCATTCAACCGTCCCCATGATCATTTACCCGCAAGGAAGGG
ATATGTTCAAAATTTCTTGCAGAAAGAAGCTGGTGACAGCCCTATCAATG
CTGCTGCGTAATTTGTGCAATTCCTTGCTCATTGGATCAATACTACTGGC
TTTAATAAATAAAGAAACTTCTGTT
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