CN181741

Overview
NameCN181741
Unique NameCN181741
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length771
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig795 contig Csv1_Contig795:23..793. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CN181741 vs. ExPASy Swiss-Prot
Match: TBB2_PEA (Tubulin beta-2 chain (Fragment) OS=Pisum sativum GN=TUBB2 PE=2 SV=1)

HSP 1 Score: 455.292 bits (1170), Expect = 1.697e-127
Identity = 217/237 (91.56%), Postives = 222/237 (93.67%), Query Frame = 2 
Query:   59 EILHIQGGQCGNQIGSKFWEVVCAEHGIDPTGKYQGDTDLQLERINVYYNEASCGRYVPRAVLMDLEPGTMDSVRSGPYGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHXXXXXXXXXXXXXXISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLSTPSFGDLNHLISATMSGVTCC 769
            EI+HIQGGQCGNQIG+KFWEVVCAEHGIDPTG+Y GDTDLQLERINVYYNEASCGRYVPRAVLMDLEPGTMDSVRSGPYGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCH              ISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKL+TPSFGDLNHLISATMSGVTCC
Sbjct:    1 EIVHIQGGQCGNQIGAKFWEVVCAEHGIDPTGRYGGDTDLQLERINVYYNEASCGRYVPRAVLMDLEPGTMDSVRSGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTCC 237
[Close]
BLAST of CN181741 vs. ExPASy Swiss-Prot
Match: TBB2_ARATH (Tubulin beta-2/beta-3 chain OS=Arabidopsis thaliana GN=TUBB2 PE=1 SV=1)

HSP 1 Score: 455.292 bits (1170), Expect = 1.697e-127
Identity = 217/239 (90.79%), Postives = 223/239 (93.31%), Query Frame = 2 
Query:   53 MREILHIQGGQCGNQIGSKFWEVVCAEHGIDPTGKYQGDTDLQLERINVYYNEASCGRYVPRAVLMDLEPGTMDSVRSGPYGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHXXXXXXXXXXXXXXISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLSTPSFGDLNHLISATMSGVTCC 769
            MREILHIQGGQCGNQIG+KFWEVVCAEHGIDPTG+Y GD+DLQLERINVYYNEASCGR+VPRAVLMDLEPGTMDS+RSGPYGQ FRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCH              ISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKL+TPSFGDLNHLISATMSGVTCC
Sbjct:    1 MREILHIQGGQCGNQIGAKFWEVVCAEHGIDPTGRYTGDSDLQLERINVYYNEASCGRFVPRAVLMDLEPGTMDSLRSGPYGQTFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTCC 239
[Close]
BLAST of CN181741 vs. ExPASy Swiss-Prot
Match: TBB8_ARATH (Tubulin beta-8 chain OS=Arabidopsis thaliana GN=TUBB8 PE=2 SV=2)

HSP 1 Score: 454.521 bits (1168), Expect = 2.894e-127
Identity = 216/239 (90.38%), Postives = 223/239 (93.31%), Query Frame = 2 
Query:   53 MREILHIQGGQCGNQIGSKFWEVVCAEHGIDPTGKYQGDTDLQLERINVYYNEASCGRYVPRAVLMDLEPGTMDSVRSGPYGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHXXXXXXXXXXXXXXISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLSTPSFGDLNHLISATMSGVTCC 769
            MREILHIQGGQCGNQIG+KFWEVVCAEHGID TG+YQG+ DLQLER+NVYYNEASCGR+VPRAVLMDLEPGTMDSVRSGPYGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCH              ISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKL+TPSFGDLNHLISATMSGVTCC
Sbjct:    1 MREILHIQGGQCGNQIGAKFWEVVCAEHGIDSTGRYQGENDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRSGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTCC 239
[Close]
BLAST of CN181741 vs. ExPASy Swiss-Prot
Match: TBB6_ARATH (Tubulin beta-6 chain OS=Arabidopsis thaliana GN=TUBB6 PE=2 SV=1)

HSP 1 Score: 452.595 bits (1163), Expect = 1.100e-126
Identity = 214/239 (89.54%), Postives = 223/239 (93.31%), Query Frame = 2 
Query:   53 MREILHIQGGQCGNQIGSKFWEVVCAEHGIDPTGKYQGDTDLQLERINVYYNEASCGRYVPRAVLMDLEPGTMDSVRSGPYGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHXXXXXXXXXXXXXXISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLSTPSFGDLNHLISATMSGVTCC 769
            MREILHIQGGQCGNQIGSKFWEVVC EHGIDPTG+Y G++DLQLER+NVYYNEASCGRYVPRA+LMDLEPGTMDSVR+GPYGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELID+VLDVVRKEAENCDCLQGFQVCH              ISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKL+TPSFGDLNHLISATMSGVTCC
Sbjct:    1 MREILHIQGGQCGNQIGSKFWEVVCDEHGIDPTGRYVGNSDLQLERVNVYYNEASCGRYVPRAILMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDAVLDVVRKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTCC 239
[Close]
BLAST of CN181741 vs. ExPASy Swiss-Prot
Match: TBB6_ORYSJ (Tubulin beta-6 chain OS=Oryza sativa subsp. japonica GN=TUBB6 PE=2 SV=1)

HSP 1 Score: 452.21 bits (1162), Expect = 1.436e-126
Identity = 215/239 (89.96%), Postives = 222/239 (92.89%), Query Frame = 2 
Query:   53 MREILHIQGGQCGNQIGSKFWEVVCAEHGIDPTGKYQGDTDLQLERINVYYNEASCGRYVPRAVLMDLEPGTMDSVRSGPYGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHXXXXXXXXXXXXXXISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLSTPSFGDLNHLISATMSGVTCC 769
            MREILHIQGGQCGNQIGSKFWEVVC EHGIDPTG+Y G +DLQLER+NVYYNEASCGR+VPRAVLMDLEPGTMDSVR+GPYGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCH              ISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKL+TPSFGDLNHLISATMSGVTCC
Sbjct:    1 MREILHIQGGQCGNQIGSKFWEVVCDEHGIDPTGRYAGTSDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTCC 239
[Close]
BLAST of CN181741 vs. ExPASy Swiss-Prot
Match: TBB6_MAIZE (Tubulin beta-6 chain OS=Zea mays GN=TUBB6 PE=2 SV=1)

HSP 1 Score: 451.825 bits (1161), Expect = 1.876e-126
Identity = 214/239 (89.54%), Postives = 223/239 (93.31%), Query Frame = 2 
Query:   53 MREILHIQGGQCGNQIGSKFWEVVCAEHGIDPTGKYQGDTDLQLERINVYYNEASCGRYVPRAVLMDLEPGTMDSVRSGPYGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHXXXXXXXXXXXXXXISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLSTPSFGDLNHLISATMSGVTCC 769
            MREILHIQGGQCGNQIG+KFWEVVC EHGIDPTG+Y G++DLQLER+NVYYNEASCGR+VPRAVLMDLEPGTMDSVR+GPYGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCH              ISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKL+TPSFGDLNHLISATMSGVTCC
Sbjct:    1 MREILHIQGGQCGNQIGAKFWEVVCDEHGIDPTGRYTGNSDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTCC 239
[Close]
BLAST of CN181741 vs. ExPASy Swiss-Prot
Match: TBB5_WHEAT (Tubulin beta-5 chain OS=Triticum aestivum GN=TUBB5 PE=2 SV=1)

HSP 1 Score: 451.825 bits (1161), Expect = 1.876e-126
Identity = 215/239 (89.96%), Postives = 222/239 (92.89%), Query Frame = 2 
Query:   53 MREILHIQGGQCGNQIGSKFWEVVCAEHGIDPTGKYQGDTDLQLERINVYYNEASCGRYVPRAVLMDLEPGTMDSVRSGPYGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHXXXXXXXXXXXXXXISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLSTPSFGDLNHLISATMSGVTCC 769
            MREILHIQGGQCGNQIGSKFWEVVC EHGIDPTG+Y G +DLQLER+NVYYNEASCGR+VPRAVLMDLEPGTMDSVR+GPYGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCH              ISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKL+TPSFGDLNHLISATMSGVTCC
Sbjct:    1 MREILHIQGGQCGNQIGSKFWEVVCDEHGIDPTGRYVGTSDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTCC 239
[Close]
BLAST of CN181741 vs. ExPASy Swiss-Prot
Match: TBB5_MAIZE (Tubulin beta-5 chain OS=Zea mays GN=TUBB5 PE=2 SV=1)

HSP 1 Score: 451.825 bits (1161), Expect = 1.876e-126
Identity = 215/239 (89.96%), Postives = 222/239 (92.89%), Query Frame = 2 
Query:   53 MREILHIQGGQCGNQIGSKFWEVVCAEHGIDPTGKYQGDTDLQLERINVYYNEASCGRYVPRAVLMDLEPGTMDSVRSGPYGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHXXXXXXXXXXXXXXISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLSTPSFGDLNHLISATMSGVTCC 769
            MREILHIQGGQCGNQIGSKFWEVVC EHGIDPTG+Y G +DLQLER+NVYYNEASCGR+VPRAVLMDLEPGTMDSVR+GPYGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCH              ISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKL+TPSFGDLNHLISATMSGVTCC
Sbjct:    1 MREILHIQGGQCGNQIGSKFWEVVCDEHGIDPTGRYVGTSDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTCC 239
[Close]
BLAST of CN181741 vs. ExPASy Swiss-Prot
Match: TBB4_ORYSJ (Tubulin beta-4 chain OS=Oryza sativa subsp. japonica GN=TUBB4 PE=2 SV=1)

HSP 1 Score: 451.825 bits (1161), Expect = 1.876e-126
Identity = 214/239 (89.54%), Postives = 223/239 (93.31%), Query Frame = 2 
Query:   53 MREILHIQGGQCGNQIGSKFWEVVCAEHGIDPTGKYQGDTDLQLERINVYYNEASCGRYVPRAVLMDLEPGTMDSVRSGPYGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHXXXXXXXXXXXXXXISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLSTPSFGDLNHLISATMSGVTCC 769
            MREILHIQGGQCGNQIG+KFWEVVC EHGIDPTG+Y G++DLQLER+NVYYNEASCGR+VPRAVLMDLEPGTMDSVR+GPYGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCH              ISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKL+TPSFGDLNHLISATMSGVTCC
Sbjct:    1 MREILHIQGGQCGNQIGAKFWEVVCDEHGIDPTGRYTGNSDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTCC 239
[Close]
BLAST of CN181741 vs. ExPASy Swiss-Prot
Match: TBB4_ELEIN (Tubulin beta-4 chain OS=Eleusine indica GN=TUBB4 PE=2 SV=1)

HSP 1 Score: 451.825 bits (1161), Expect = 1.876e-126
Identity = 215/239 (89.96%), Postives = 222/239 (92.89%), Query Frame = 2 
Query:   53 MREILHIQGGQCGNQIGSKFWEVVCAEHGIDPTGKYQGDTDLQLERINVYYNEASCGRYVPRAVLMDLEPGTMDSVRSGPYGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHXXXXXXXXXXXXXXISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLSTPSFGDLNHLISATMSGVTCC 769
            MREILHIQGGQCGNQIGSKFWEVVC EHGIDPTG+Y G +DLQLER+NVYYNEASCGR+VPRAVLMDLEPGTMDSVR+GPYGQ+FRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCH              ISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKL+TPSFGDLNHLISATMSGVTCC
Sbjct:    1 MREILHIQGGQCGNQIGSKFWEVVCDEHGIDPTGRYVGTSDLQLERVNVYYNEASCGRFVPRAVLMDLEPGTMDSVRTGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTCC 239
The following BLAST results are available for this feature:
BLAST of CN181741 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 423
Match NameE-valueIdentityDescription
TBB2_PEA1.697e-12791.56Tubulin beta-2 chain (Fragment) OS=Pisum sativum G... [more]
TBB2_ARATH1.697e-12790.79Tubulin beta-2/beta-3 chain OS=Arabidopsis thalian... [more]
TBB8_ARATH2.894e-12790.38Tubulin beta-8 chain OS=Arabidopsis thaliana GN=TU... [more]
TBB6_ARATH1.100e-12689.54Tubulin beta-6 chain OS=Arabidopsis thaliana GN=TU... [more]
TBB6_ORYSJ1.436e-12689.96Tubulin beta-6 chain OS=Oryza sativa subsp. japoni... [more]
TBB6_MAIZE1.876e-12689.54Tubulin beta-6 chain OS=Zea mays GN=TUBB6 PE=2 SV=... [more]
TBB5_WHEAT1.876e-12689.96Tubulin beta-5 chain OS=Triticum aestivum GN=TUBB5... [more]
TBB5_MAIZE1.876e-12689.96Tubulin beta-5 chain OS=Zea mays GN=TUBB5 PE=2 SV=... [more]
TBB4_ORYSJ1.876e-12689.54Tubulin beta-4 chain OS=Oryza sativa subsp. japoni... [more]
TBB4_ELEIN1.876e-12689.96Tubulin beta-4 chain OS=Eleusine indica GN=TUBB4 P... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0001G13_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0001G13, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN181741 ID=CN181741; Name=CN181741; organism=Citrus sinensis; type=EST; length=771bp
GTGAAAATATCCTCTGCAAATCTCGCTAATCAAGAAACCCCAAAAGAAGA
AAATGCGTGAAATCCTTCACATTCAAGGGGGCCAATGCGGGAACCAAATC
GGATCCAAGTTCTGGGAAGTGGTTTGCGCCGAGCACGGTATAGACCCGAC
CGGAAAGTATCAAGGGGATACCGATTTGCAGCTTGAGAGGATTAATGTGT
ATTACAACGAGGCAAGTTGCGGGAGATACGTGCCTAGGGCCGTGCTCATG
GATCTTGAACCTGGGACCATGGATAGCGTCAGGTCTGGTCCGTACGGTCA
GGTTTTTAGGCCCGACAATTTTGTCTTTGGCCAGTCCGGCGCCGGAAACA
ACTGGGCGAAGGGGCATTATACTGAAGGAGCTGAGCTGATTGACTCTGTG
CTTGATGTTGTTCGTAAAGAAGCCGAGAACTGCGATTGCTTGCAGGGATT
TCAGGTGTGCCATTCTCTGGGAGGAGGGACAGGTTCCGGGATGGGAACTT
TGTTGATATCAAAGATAAGAGAGGAATACCCGGATAGAATGATGCTTACT
TTCTCAGTTTTCCCATCTCCTAAGGTCTCTGATACTGTTGTTGAGCCTTA
CAACGCTACTCTCTCTGTACATCAGCTAGTTGAAAATGCAGATGAGTGTA
TGGTTCTTGATAATGAAGCCCTTTACGACATCTGTTTCCGCACTCTCAAA
CTCAGCACTCCAAGCTTTGGTGATCTGAATCATTTGATATCTGCTACAAT
GTCTGGAGTTACCTGTTGTTT
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