CN181546

Overview
NameCN181546
Unique NameCN181546
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length674
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig13395 contig Csv1_Contig13395:17..690. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CN181546 vs. ExPASy Swiss-Prot
Match: LGUL_BRAOG (Putative lactoylglutathione lyase OS=Brassica oleracea var. gemmifera PE=2 SV=1)

HSP 1 Score: 350.132 bits (897), Expect = 6.035e-96
Identity = 166/201 (82.59%), Postives = 186/201 (92.54%), Query Frame = 1 
Query:   70 WPKKDKRRFLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPLKGGTTHIAFVKDPDGYIFELIQRGPTPEPLCQVMLRVGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGVTE 672
            WPKKDKRRFLH VYRVGDLDRTI+FYTECFGMK+LRKRDVPEEKYSNAFLGFGPE S+FVVELTYNYGV+SYDIGTGFGHFAI+T+DV K+VE +RAKGGNVTREPGP+KGG + IAFVKDPDGY FELIQRGPTPEPLCQVMLRVGDL R++KF EKALGM+LLR +++PEY  T+ M+GYAEE ++ VLELTYNYGVTE
Sbjct:   10 WPKKDKRRFLHVVYRVGDLDRTIQFYTECFGMKVLRKRDVPEEKYSNAFLGFGPETSNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKMVEAVRAKGGNVTREPGPVKGGGSVIAFVKDPDGYTFELIQRGPTPEPLCQVMLRVGDLDRAVKFMEKALGMRLLRRIERPEYN-TIGMMGYAEEYESIVLELTYNYGVTE 209

HSP 2 Score: 99.7525 bits (247), Expect = 1.421e-20
Identity = 53/115 (46.09%), Postives = 68/115 (59.13%), Query Frame = 1 
Query:  112 RVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIR----AKGGNVTREPGPLKGGTTHIAFVKDPDGY 444
            RVGDLDR +KF  +  GM+LLR+ + PE   +   +G+  E    V+ELTYNYGVT Y  G  +   AI T+DVYK  E ++      GG +TRE GPL G  T I    DPDG+
Sbjct:  154 RVGDLDRAVKFMEKALGMRLLRRIERPEYN-TIGMMGYAEEYESIVLELTYNYGVTEYTKGNAYAQIAIGTDDVYKSAEVVKIVNQELGGKITREAGPLPGLGTKIVSFLDPDGW 267
[Close]
BLAST of CN181546 vs. ExPASy Swiss-Prot
Match: LGUL_ORYSJ (Lactoylglutathione lyase OS=Oryza sativa subsp. japonica GN=GLX-I PE=1 SV=2)

HSP 1 Score: 346.28 bits (887), Expect = 8.714e-95
Identity = 165/202 (81.68%), Postives = 182/202 (90.10%), Query Frame = 1 
Query:   70 WPKKDKRRFLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGG-NVTREPGPLKGGTTHIAFVKDPDGYIFELIQRGPTPEPLCQVMLRVGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGVTE 672
            WPKKDK+R LHAVYRVGDLDRTIK YTECFGMKLLRKRDVPEEKY+NAFLGFGPE ++F +ELTYNYGV  YDIG GFGHFAIATEDVYKL E I++     +TREPGP+KGG+T IAF +DPDGY+FELIQRGPTPEPLCQVMLRVGDL RSIKFYEKALGMKLLR  D P+YKYT+AMLGYA+ED+TTV+ELTYNYGVTE
Sbjct:   17 WPKKDKKRLLHAVYRVGDLDRTIKCYTECFGMKLLRKRDVPEEKYTNAFLGFGPEDTNFALELTYNYGVDKYDIGAGFGHFAIATEDVYKLAEKIKSSCCCKITREPGPVKGGSTVIAFAQDPDGYMFELIQRGPTPEPLCQVMLRVGDLDRSIKFYEKALGMKLLRKKDVPDYKYTIAMLGYADEDKTTVIELTYNYGVTE 218

HSP 2 Score: 125.946 bits (315), Expect = 1.851e-28
Identity = 64/120 (53.33%), Postives = 78/120 (65.00%), Query Frame = 1 
Query:  112 RVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENI----RAKGGNVTREPGPLKGGTTHIAFVKDPDGYIFELI 459
            RVGDLDR+IKFY +  GMKLLRK+DVP+ KY+ A LG+  E    V+ELTYNYGVT Y  G  +   AI TEDVYK  E +    +  GG + R+PGPL G  T IA   DPDG+   L+
Sbjct:  162 RVGDLDRSIKFYEKALGMKLLRKKDVPDYKYTIAMLGYADEDKTTVIELTYNYGVTEYTKGNAYAQVAIGTEDVYKSAEAVELVTKELGGKILRQPGPLPGLNTKIASFLDPDGWKVVLV 281
[Close]
BLAST of CN181546 vs. ExPASy Swiss-Prot
Match: LGUL_VIBPA (Probable lactoylglutathione lyase OS=Vibrio parahaemolyticus GN=gloA PE=3 SV=2)

HSP 1 Score: 166.777 bits (421), Expect = 9.462e-41
Identity = 77/124 (62.10%), Postives = 95/124 (76.61%), Query Frame = 1 
Query:   91 RFLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPLKGGTTHIAFVKDPDGYIFELIQ 462
            R LH + RVGDLD++IKFYTE  GM+LLR  +  E +Y+ AF+G+G E    V+ELTYN+G T YD+GT FGH AI  +D+Y   + I+A GGNVTRE GP+KGGTTHIAFVKDPDGY+ ELIQ
Sbjct:    5 RILHTMLRVGDLDKSIKFYTEVMGMQLLRTNENKEYEYTLAFVGYGDESQGAVIELTYNWGKTEYDLGTAFGHIAIGVDDIYATCDAIKAAGGNVTREAGPVKGGTTHIAFVKDPDGYMIELIQ 128

HSP 2 Score: 80.8777 bits (198), Expect = 6.829e-15
Identity = 37/63 (58.73%), Postives = 48/63 (76.19%), Query Frame = 1 
Query:  484 LCQVMLRVGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGVTE 672
            +   MLRVGDL +SIKFY + +GM+LLRT +  EY+YTLA +GY +E Q  V+ELTYN+G TE
Sbjct:    6 ILHTMLRVGDLDKSIKFYTEVMGMQLLRTNENKEYEYTLAFVGYGDESQGAVIELTYNWGKTE 68
[Close]
BLAST of CN181546 vs. ExPASy Swiss-Prot
Match: LGUL_VIBCH (Probable lactoylglutathione lyase OS=Vibrio cholerae GN=gloA PE=3 SV=2)

HSP 1 Score: 162.925 bits (411), Expect = 1.366e-39
Identity = 75/124 (60.48%), Postives = 93/124 (75.00%), Query Frame = 1 
Query:   91 RFLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPLKGGTTHIAFVKDPDGYIFELIQ 462
            R LH + RVGDLD++I+FYT+  GM LLRK +  E KY+ AFLG+G E    V+ELTYN+GV  Y+ G  +GH AI  +D+Y   + I+A GG VTREPGP+KGGTTHIAFVKDPDGY+ ELIQ
Sbjct:    5 RILHTMLRVGDLDKSIEFYTQVMGMSLLRKNENTEYKYTLAFLGYGDESQGAVIELTYNWGVADYEKGNAYGHIAIGVDDIYATCDTIKAAGGIVTREPGPVKGGTTHIAFVKDPDGYMIELIQ 128

HSP 2 Score: 78.5666 bits (192), Expect = 3.389e-14
Identity = 36/63 (57.14%), Postives = 46/63 (73.02%), Query Frame = 1 
Query:  484 LCQVMLRVGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGVTE 672
            +   MLRVGDL +SI+FY + +GM LLR  +  EYKYTLA LGY +E Q  V+ELTYN+GV +
Sbjct:    6 ILHTMLRVGDLDKSIEFYTQVMGMSLLRKNENTEYKYTLAFLGYGDESQGAVIELTYNWGVAD 68
[Close]
BLAST of CN181546 vs. ExPASy Swiss-Prot
Match: LGUL_SHIFL (Lactoylglutathione lyase OS=Shigella flexneri GN=gloA PE=3 SV=1)

HSP 1 Score: 156.377 bits (394), Expect = 1.279e-37
Identity = 73/124 (58.87%), Postives = 92/124 (74.19%), Query Frame = 1 
Query:   91 RFLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPLKGGTTHIAFVKDPDGYIFELIQ 462
            R LH + RVGDL R+I FYT+  GMKLLR  + PE KYS AF+G+GPE    V+ELTYN+GV  Y++GT +GH A++ ++  +  E IR  GGNVTRE GP+KGGTT IAFV+DPDGY  ELI+
Sbjct:    2 RLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGPVKGGTTVIAFVEDPDGYKIELIE 125

HSP 2 Score: 83.5741 bits (205), Expect = 1.054e-15
Identity = 40/61 (65.57%), Postives = 46/61 (75.41%), Query Frame = 1 
Query:  484 LCQVMLRVGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGV 666
            L   MLRVGDL RSI FY K LGMKLLRT + PEYKY+LA +GY  E +  V+ELTYN+GV
Sbjct:    3 LLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGV 63
[Close]
BLAST of CN181546 vs. ExPASy Swiss-Prot
Match: LGUL_ECOLI (Lactoylglutathione lyase OS=Escherichia coli (strain K12) GN=gloA PE=1 SV=1)

HSP 1 Score: 156.377 bits (394), Expect = 1.279e-37
Identity = 73/124 (58.87%), Postives = 92/124 (74.19%), Query Frame = 1 
Query:   91 RFLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPLKGGTTHIAFVKDPDGYIFELIQ 462
            R LH + RVGDL R+I FYT+  GMKLLR  + PE KYS AF+G+GPE    V+ELTYN+GV  Y++GT +GH A++ ++  +  E IR  GGNVTRE GP+KGGTT IAFV+DPDGY  ELI+
Sbjct:    2 RLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGPVKGGTTVIAFVEDPDGYKIELIE 125

HSP 2 Score: 83.5741 bits (205), Expect = 1.054e-15
Identity = 40/61 (65.57%), Postives = 46/61 (75.41%), Query Frame = 1 
Query:  484 LCQVMLRVGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGV 666
            L   MLRVGDL RSI FY K LGMKLLRT + PEYKY+LA +GY  E +  V+ELTYN+GV
Sbjct:    3 LLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGV 63
[Close]
BLAST of CN181546 vs. ExPASy Swiss-Prot
Match: LGUL_ECO57 (Lactoylglutathione lyase OS=Escherichia coli O157:H7 GN=gloA PE=3 SV=1)

HSP 1 Score: 156.377 bits (394), Expect = 1.279e-37
Identity = 73/124 (58.87%), Postives = 92/124 (74.19%), Query Frame = 1 
Query:   91 RFLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPLKGGTTHIAFVKDPDGYIFELIQ 462
            R LH + RVGDL R+I FYT+  GMKLLR  + PE KYS AF+G+GPE    V+ELTYN+GV  Y++GT +GH A++ ++  +  E IR  GGNVTRE GP+KGGTT IAFV+DPDGY  ELI+
Sbjct:    2 RLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGPVKGGTTVIAFVEDPDGYKIELIE 125

HSP 2 Score: 83.5741 bits (205), Expect = 1.054e-15
Identity = 40/61 (65.57%), Postives = 46/61 (75.41%), Query Frame = 1 
Query:  484 LCQVMLRVGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGV 666
            L   MLRVGDL RSI FY K LGMKLLRT + PEYKY+LA +GY  E +  V+ELTYN+GV
Sbjct:    3 LLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGV 63
[Close]
BLAST of CN181546 vs. ExPASy Swiss-Prot
Match: LGUL_SALTY (Lactoylglutathione lyase OS=Salmonella typhimurium GN=gloA PE=3 SV=1)

HSP 1 Score: 155.221 bits (391), Expect = 2.849e-37
Identity = 73/124 (58.87%), Postives = 91/124 (73.39%), Query Frame = 1 
Query:   91 RFLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPLKGGTTHIAFVKDPDGYIFELIQ 462
            R LH + RVGDL R+I FYT   GMKLLR  + PE KYS AF+G+GPE    V+ELTYN+GV SYD+G  +GH A++ ++  +  E IR  GGNVTRE GP+KGG+T IAFV+DPDGY  ELI+
Sbjct:    2 RLLHTMLRVGDLQRSIAFYTNVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVESYDMGNAYGHIALSVDNAAEACERIRQNGGNVTREAGPVKGGSTIIAFVEDPDGYKIELIE 125

HSP 2 Score: 81.6481 bits (200), Expect = 4.003e-15
Identity = 39/61 (63.93%), Postives = 45/61 (73.77%), Query Frame = 1 
Query:  484 LCQVMLRVGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGV 666
            L   MLRVGDL RSI FY   LGMKLLRT + PEYKY+LA +GY  E +  V+ELTYN+GV
Sbjct:    3 LLHTMLRVGDLQRSIAFYTNVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGV 63
[Close]
BLAST of CN181546 vs. ExPASy Swiss-Prot
Match: LGUL_SALTI (Lactoylglutathione lyase OS=Salmonella typhi GN=gloA PE=3 SV=1)

HSP 1 Score: 155.221 bits (391), Expect = 2.849e-37
Identity = 73/124 (58.87%), Postives = 91/124 (73.39%), Query Frame = 1 
Query:   91 RFLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPLKGGTTHIAFVKDPDGYIFELIQ 462
            R LH + RVGDL R+I FYT   GMKLLR  + PE KYS AF+G+GPE    V+ELTYN+GV SYD+G  +GH A++ ++  +  E IR  GGNVTRE GP+KGG+T IAFV+DPDGY  ELI+
Sbjct:    2 RLLHTMLRVGDLQRSIAFYTNVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGVESYDMGNAYGHIALSVDNAAEACERIRQNGGNVTREAGPVKGGSTIIAFVEDPDGYKIELIE 125

HSP 2 Score: 81.6481 bits (200), Expect = 4.003e-15
Identity = 39/61 (63.93%), Postives = 45/61 (73.77%), Query Frame = 1 
Query:  484 LCQVMLRVGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYGV 666
            L   MLRVGDL RSI FY   LGMKLLRT + PEYKY+LA +GY  E +  V+ELTYN+GV
Sbjct:    3 LLHTMLRVGDLQRSIAFYTNVLGMKLLRTSENPEYKYSLAFVGYGPETEEAVIELTYNWGV 63
[Close]
BLAST of CN181546 vs. ExPASy Swiss-Prot
Match: LGUL_SYNY3 (Probable lactoylglutathione lyase OS=Synechocystis sp. (strain PCC 6803) GN=gloA PE=3 SV=1)

HSP 1 Score: 153.295 bits (386), Expect = 1.083e-36
Identity = 68/122 (55.74%), Postives = 90/122 (73.77%), Query Frame = 1 
Query:   97 LHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEQSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPLKGGTTHIAFVKDPDGYIFELIQ 462
            LH + RVGDLD++++FY +  GM LLRK+D P  +++ AF+G+G E  + V+ELT+N+G   YD+G GFGH A+  ED+Y   + IR KGG V REPGP+K GTT IAFV+DPDGY  ELIQ
Sbjct:    4 LHTMIRVGDLDKSLQFYCDILGMNLLRKKDYPSGEFTLAFVGYGKESENAVIELTHNWGTDKYDLGNGFGHIALGVEDIYSTCDKIRDKGGKVVREPGPMKHGTTVIAFVEDPDGYKIELIQ 125
The following BLAST results are available for this feature:
BLAST of CN181546 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 28
Match NameE-valueIdentityDescription
LGUL_BRAOG6.035e-9682.59Putative lactoylglutathione lyase OS=Brassica oler... [more]
LGUL_ORYSJ8.714e-9581.68Lactoylglutathione lyase OS=Oryza sativa subsp. ja... [more]
LGUL_VIBPA9.462e-4162.10Probable lactoylglutathione lyase OS=Vibrio paraha... [more]
LGUL_VIBCH1.366e-3960.48Probable lactoylglutathione lyase OS=Vibrio choler... [more]
LGUL_SHIFL1.279e-3758.87Lactoylglutathione lyase OS=Shigella flexneri GN=g... [more]
LGUL_ECOLI1.279e-3758.87Lactoylglutathione lyase OS=Escherichia coli (stra... [more]
LGUL_ECO571.279e-3758.87Lactoylglutathione lyase OS=Escherichia coli O157:... [more]
LGUL_SALTY2.849e-3758.87Lactoylglutathione lyase OS=Salmonella typhimurium... [more]
LGUL_SALTI2.849e-3758.87Lactoylglutathione lyase OS=Salmonella typhi GN=gl... [more]
LGUL_SYNY31.083e-3655.74Probable lactoylglutathione lyase OS=Synechocystis... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0001B17_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0001B17, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN181546 ID=CN181546; Name=CN181546; organism=Citrus sinensis; type=EST; length=674bp
GAGGCTGAGTGATTCCATATCAAAATGGCTGAGGCTTCTCCTGCTGCTGC
TAATGCCGAGTTGCTGGAATGGCCAAAGAAAGATAAGCGACGCTTCCTTC
ATGCTGTGTATCGTGTTGGTGATCTTGATCGCACCATCAAATTTTACACC
GAATGCTTTGGGATGAAGCTGTTGAGAAAGAGAGACGTCCCGGAAGAGAA
ATACTCAAATGCCTTTCTTGGATTTGGCCCCGAACAGTCTCACTTTGTTG
TGGAGTTAACGTACAACTATGGTGTAACTTCATATGATATTGGAACTGGT
TTTGGCCATTTTGCAATTGCAACTGAAGATGTTTACAAACTGGTTGAAAA
CATTCGTGCCAAGGGTGGCAATGTCACTAGAGAGCCTGGTCCACTTAAAG
GTGGAACAACTCACATTGCCTTTGTGAAGGATCCCGATGGTTATATTTTT
GAACTCATCCAGAGAGGTCCAACTCCTGAACCACTCTGTCAAGTAATGCT
TCGCGTTGGCGATCTTGGTCGCTCCATCAAGTTCTATGAAAAGGCTTTGG
GGATGAAACTGTTAAGAACAGTTGATAAACCTGAATACAAGTACACCCTG
GCCATGCTTGGATATGCTGAGGAAGATCAGACTACTGTTCTGGAGTTAAC
TTATAATTATGGTGTGACTGAATA
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