CN187847

Overview
NameCN187847
Unique NameCN187847
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length729
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CN187847_ssr47 microsatellite CN187847_ssr47:47..58. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig2623 contig Csv1_Contig2623:1..729. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CN187847 vs. ExPASy Swiss-Prot
Match: MED10_AEDAE (Mediator of RNA polymerase II transcription subunit 10 OS=Aedes aegypti GN=MED10 PE=3 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 9.021e-19
Identity = 45/129 (34.88%), Postives = 78/129 (60.47%), Query Frame = 1 
Query:  190 PKQNLNQVINSVQKTLGLLHQLYLTVSSFNAASQLPLLQRLNSLVSELDNMVKLSEKCNIQVPMEVLNLIDDGKNPDEFTRDVINSCIAKNQVTKGKTDAFKSLRKHLLDELEQTFPDEVEAYREIRAN 576
            P +NL    N ++  +  + Q+ + VS F    Q  L Q++ SLV+ L  + KL  + ++ VP+EV + ID G+NP  +T+D I+  + KN+  KGK D+++  + +L+ EL++TFP E+  Y+ IR +
Sbjct:    4 PLENLE---NHLEMFIENVRQIRIIVSDFQPQGQNVLNQKIQSLVTGLQEIDKLKNQVDVNVPLEVFDYIDQGRNPQLYTKDCIDKALTKNEEVKGKIDSYRKFKSNLMKELDETFPTEIAKYKAIRGD 129
[Close]
BLAST of CN187847 vs. ExPASy Swiss-Prot
Match: MED10_ANOGA (Mediator of RNA polymerase II transcription subunit 10 OS=Anopheles gambiae GN=MED10 PE=3 SV=2)

HSP 1 Score: 92.8189 bits (229), Expect = 2.010e-18
Identity = 45/129 (34.88%), Postives = 77/129 (59.69%), Query Frame = 1 
Query:  190 PKQNLNQVINSVQKTLGLLHQLYLTVSSFNAASQLPLLQRLNSLVSELDNMVKLSEKCNIQVPMEVLNLIDDGKNPDEFTRDVINSCIAKNQVTKGKTDAFKSLRKHLLDELEQTFPDEVEAYREIRAN 576
            P +NL    N ++  +  + Q+ + VS F    Q  L Q++ SLV+ L  + KL  + ++ VP+EV + ID G+NP  +T+D I+  + KN+  KGK D+++  + +L+ EL +TFP E+  Y+ IR +
Sbjct:    4 PLENLE---NHLEMFIENVRQIRIIVSDFQPQGQNVLNQKIQSLVTGLQEIDKLKNQIDVNVPLEVFDYIDQGRNPQLYTKDCIDKALTKNEEVKGKIDSYRKFKSNLMKELSETFPVEISKYKAIRGD 129
[Close]
BLAST of CN187847 vs. ExPASy Swiss-Prot
Match: MED10_MOUSE (Mediator of RNA polymerase II transcription subunit 10 OS=Mus musculus GN=Med10 PE=1 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 4.190e-16
Identity = 43/117 (36.75%), Postives = 71/117 (60.68%), Query Frame = 1 
Query:  223 VQKTLGLLHQLYLTVSSFNAASQLPLLQRLNSLVSELDNMVKLSEKCN-IQVPMEVLNLIDDGKNPDEFTRDVINSCIAKNQVTKGKTDAFKSLRKHLLDELEQTFPDEVEAYREIR 570
            ++K +  + QL + VS F  +SQ  L Q+LN +V+ L ++ K  ++ + I VP+EV   ID G+NP  +T++ +   +AKN+  KGK D  K  +  L+ EL + FP+++  YR IR
Sbjct:   12 LEKFVENIRQLGIIVSDFQPSSQAGLSQKLNFIVTGLQDIDKCRQQLHDITVPLEVFEYIDQGRNPQLYTKECLERALAKNEQVKGKIDTMKKFKSLLIQELSKVFPEDMAKYRSIR 128
[Close]
BLAST of CN187847 vs. ExPASy Swiss-Prot
Match: MED10_PONAB (Mediator of RNA polymerase II transcription subunit 10 OS=Pongo abelii GN=MED10 PE=2 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 5.473e-16
Identity = 43/117 (36.75%), Postives = 71/117 (60.68%), Query Frame = 1 
Query:  223 VQKTLGLLHQLYLTVSSFNAASQLPLLQRLNSLVSELDNMVKLSEKCN-IQVPMEVLNLIDDGKNPDEFTRDVINSCIAKNQVTKGKTDAFKSLRKHLLDELEQTFPDEVEAYREIR 570
            ++K +  + QL + VS F  +SQ  L Q+LN +V+ L ++ K  ++ + I VP+EV   ID G+NP  +T++ +   +AKN+  KGK D  K  +  L+ EL + FP+++  YR IR
Sbjct:   12 LEKFVENIRQLGIIVSDFQPSSQAGLNQKLNFIVTGLQDIDKCRQQLHDITVPLEVFEYIDQGRNPQLYTKECLERALAKNEQVKGKIDTMKKFKSLLIQELSKVFPEDMAKYRSIR 128
[Close]
BLAST of CN187847 vs. ExPASy Swiss-Prot
Match: MED10_HUMAN (Mediator of RNA polymerase II transcription subunit 10 OS=Homo sapiens GN=MED10 PE=1 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 5.473e-16
Identity = 43/117 (36.75%), Postives = 71/117 (60.68%), Query Frame = 1 
Query:  223 VQKTLGLLHQLYLTVSSFNAASQLPLLQRLNSLVSELDNMVKLSEKCN-IQVPMEVLNLIDDGKNPDEFTRDVINSCIAKNQVTKGKTDAFKSLRKHLLDELEQTFPDEVEAYREIR 570
            ++K +  + QL + VS F  +SQ  L Q+LN +V+ L ++ K  ++ + I VP+EV   ID G+NP  +T++ +   +AKN+  KGK D  K  +  L+ EL + FP+++  YR IR
Sbjct:   12 LEKFVENIRQLGIIVSDFQPSSQAGLNQKLNFIVTGLQDIDKCRQQLHDITVPLEVFEYIDQGRNPQLYTKECLERALAKNEQVKGKIDTMKKFKSLLIQELSKVFPEDMAKYRSIR 128
[Close]
BLAST of CN187847 vs. ExPASy Swiss-Prot
Match: MED10_BOVIN (Mediator of RNA polymerase II transcription subunit 10 OS=Bos taurus GN=MED10 PE=2 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 5.473e-16
Identity = 43/117 (36.75%), Postives = 71/117 (60.68%), Query Frame = 1 
Query:  223 VQKTLGLLHQLYLTVSSFNAASQLPLLQRLNSLVSELDNMVKLSEKCN-IQVPMEVLNLIDDGKNPDEFTRDVINSCIAKNQVTKGKTDAFKSLRKHLLDELEQTFPDEVEAYREIR 570
            ++K +  + QL + VS F  +SQ  L Q+LN +V+ L ++ K  ++ + I VP+EV   ID G+NP  +T++ +   +AKN+  KGK D  K  +  L+ EL + FP+++  YR IR
Sbjct:   12 LEKFVENIRQLGIIVSDFQPSSQAGLNQKLNFIVTGLQDIDKCRQQLHDITVPLEVFEYIDQGRNPQLYTKECLERALAKNEQVKGKIDTMKKFKSLLIQELSKVFPEDMAKYRSIR 128
[Close]
BLAST of CN187847 vs. ExPASy Swiss-Prot
Match: MED10_XENTR (Mediator of RNA polymerase II transcription subunit 10 OS=Xenopus tropicalis GN=med10 PE=2 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 7.148e-16
Identity = 43/117 (36.75%), Postives = 72/117 (61.54%), Query Frame = 1 
Query:  223 VQKTLGLLHQLYLTVSSFNAASQLPLLQRLNSLVSELDNMVKLSEKCN-IQVPMEVLNLIDDGKNPDEFTRDVINSCIAKNQVTKGKTDAFKSLRKHLLDELEQTFPDEVEAYREIR 570
            ++K +  + QL + VS F  +SQ  L Q+LN LV+ L ++ K  ++ + I VP+EV + ID G+NP  +T++ +   +AKN+  KGK D  K  +  L+ EL + FP+++  Y+ IR
Sbjct:   12 LEKFVENIRQLGIIVSDFQPSSQAGLNQKLNFLVTGLQDIDKCRQQLHDITVPLEVFDYIDQGRNPQLYTKECLERALAKNEQVKGKIDTLKKFKSLLIQELSKVFPEDMAKYKAIR 128
[Close]
BLAST of CN187847 vs. ExPASy Swiss-Prot
Match: MED10_XENLA (Mediator of RNA polymerase II transcription subunit 10 OS=Xenopus laevis GN=med10 PE=2 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 1.219e-15
Identity = 42/126 (33.33%), Postives = 75/126 (59.52%), Query Frame = 1 
Query:  196 QNLNQVINSVQKTLGLLHQLYLTVSSFNAASQLPLLQRLNSLVSELDNMVKLSEKCN-IQVPMEVLNLIDDGKNPDEFTRDVINSCIAKNQVTKGKTDAFKSLRKHLLDELEQTFPDEVEAYREIR 570
            +  + + + ++K +  + QL + VS F  +SQ  L Q+LN LV  L ++ K  ++ + I VP+EV + ID G+NP  +T++ +   +AKN+  KGK D  K  +  L+ EL + FP+++  Y+ +R
Sbjct:    3 EKFDNLEDHLEKFVENIRQLGIIVSDFQPSSQAGLNQKLNFLVRGLQDIDKCRQQLHDITVPLEVFDYIDQGRNPQLYTKECLERALAKNEQVKGKIDTLKKFKSLLIQELSKVFPEDMAKYKAVR 128
[Close]
BLAST of CN187847 vs. ExPASy Swiss-Prot
Match: MED10_MACFA (Mediator of RNA polymerase II transcription subunit 10 OS=Macaca fascicularis GN=MED10 PE=2 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 3.547e-15
Identity = 42/117 (35.90%), Postives = 70/117 (59.83%), Query Frame = 1 
Query:  223 VQKTLGLLHQLYLTVSSFNAASQLPLLQRLNSLVSELDNMVKLSEKCN-IQVPMEVLNLIDDGKNPDEFTRDVINSCIAKNQVTKGKTDAFKSLRKHLLDELEQTFPDEVEAYREIR 570
            ++K +  + QL + VS F  +SQ  L Q+LN +V+ L ++ K  ++ + I VP+EV   ID G+NP  +T++ +   +AKN+  KGK D  K  +  L+  L + FP+++  YR IR
Sbjct:   12 LEKFVENIRQLGIIVSDFQPSSQAGLNQKLNFIVTGLQDIDKCRQQLHDITVPLEVFEYIDQGRNPQLYTKECLERALAKNEQVKGKIDTMKKFKSLLIQGLSKVFPEDMAKYRSIR 128
[Close]
BLAST of CN187847 vs. ExPASy Swiss-Prot
Match: MED10_DANRE (Mediator of RNA polymerase II transcription subunit 10 OS=Danio rerio GN=med10 PE=2 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 1.348e-14
Identity = 40/119 (33.61%), Postives = 70/119 (58.82%), Query Frame = 1 
Query:  223 VQKTLGLLHQLYLTVSSFNAASQLPLLQRLNSLVSELDNMVKLSEKCN-IQVPMEVLNLIDDGKNPDEFTRDVINSCIAKNQVTKGKTDAFKSLRKHLLDELEQTFPDEVEAYREIRAN 576
            ++K +  + QL + VS F  +SQ  L Q+LN +++ L ++ K  ++ + I VP+EV   ID G+NP  +T++ +   +AKN+  KGK D     +  L+ EL + FP+E+  Y+ I  +
Sbjct:   12 LEKFVENIRQLGIIVSDFQPSSQTGLNQKLNFMITGLQDIEKCRQQLHDINVPLEVFEYIDQGRNPQLYTKECLERALAKNEQVKGKIDTLTKFKSLLISELGKVFPEEMAKYKAIHGD 130
The following BLAST results are available for this feature:
BLAST of CN187847 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 12
Match NameE-valueIdentityDescription
MED10_AEDAE9.021e-1934.88Mediator of RNA polymerase II transcription subuni... [more]
MED10_ANOGA2.010e-1834.88Mediator of RNA polymerase II transcription subuni... [more]
MED10_MOUSE4.190e-1636.75Mediator of RNA polymerase II transcription subuni... [more]
MED10_PONAB5.473e-1636.75Mediator of RNA polymerase II transcription subuni... [more]
MED10_HUMAN5.473e-1636.75Mediator of RNA polymerase II transcription subuni... [more]
MED10_BOVIN5.473e-1636.75Mediator of RNA polymerase II transcription subuni... [more]
MED10_XENTR7.148e-1636.75Mediator of RNA polymerase II transcription subuni... [more]
MED10_XENLA1.219e-1533.33Mediator of RNA polymerase II transcription subuni... [more]
MED10_MACFA3.547e-1535.90Mediator of RNA polymerase II transcription subuni... [more]
MED10_DANRE1.348e-1433.61Mediator of RNA polymerase II transcription subuni... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionUCRCS05_0005B14_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0005B14, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN187847 ID=CN187847; Name=CN187847; organism=Citrus sinensis; type=EST; length=729bp
AGTACCCGGTCTTCTAAATAGCGATTCGCAGAAGTTTTGGGGTCCTGAAG
AAGAAGAAAACTGTAATTACAGTTTCCTGATACGAGAGAAAGAAAGATGG
ATGGACCGGTGGGGGGCAGCAGAGCAAGTGGTGGCAATGGAATGGTGAGC
AATCAAGCAAACGATACAACAACTGTGGCAGCTGACGATCCGAAGCAGAA
CTTGAACCAGGTTATTAATTCAGTTCAGAAGACTCTTGGGCTCCTACATC
AACTCTACCTTACCGTATCTTCCTTCAATGCTGCCTCCCAGCTCCCTCTC
CTACAAAGACTCAATTCTCTTGTTTCGGAGCTTGACAACATGGTTAAGTT
GTCTGAGAAGTGCAATATTCAAGTGCCCATGGAAGTGCTTAATTTGATTG
ATGACGGAAAAAATCCTGATGAATTCACAAGGGATGTTATAAACAGCTGC
ATTGCTAAAAACCAAGTCACCAAAGGAAAAACTGATGCTTTCAAGAGTTT
ACGTAAGCATCTTTTGGACGAACTTGAGCAGACATTTCCGGATGAAGTTG
AAGCATATAGAGAGATACGTGCAAATTCTGCTGCTGAAGCAAAGCGTCTT
GCTCAATCACAAAGTATGTTGCCAAATGGAGATGTGAAAGTTAAATCTGA
GCTTTAAATGTCCCATTTCATGCAGAGTGTTGGGGCTCTTTCGACTACTA
CTTTTCTCAATGTAATGTCATGAGTCGTT
back to top