CK937612 | Citrus Genome Database

Overview


Name	CK937612
Unique Name	CK937612
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	766

Libraries


Library Name	Type
Developing fruit albedo at 80 DAFB in pTriplEx2 vector	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CK937612 vs. ExPASy Swiss-Prot
Match: IDHP_MEDSA (Isocitrate dehydrogenase [NADP], chloroplastic (Fragment) OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 7.769e-28
Identity = 39/50 (78.00%), Postives = 47/50 (94.00%), Query Frame = 3

Query:  480 QLIFPYLDLDIKYYDLGILNRDATDDKVTVESAESTLKYNVAIKCATITP 629
            +LIFP+++LDIKY+DLG+  RD T+DKVTVESAE+TLKYNVAIKCATITP
Sbjct:   52 KLIFPFVELDIKYFDLGLPYRDETNDKVTVESAEATLKYNVAIKCATITP 101

HSP 2 Score: 60.8474 bits (146), Expect = 7.769e-28
Identity = 29/58 (50.00%), Postives = 38/58 (65.52%), Query Frame = 2

Query:  239 QLAPAMSFRCF-------ASATGIDRVKVQNPIVEMDGDEMTRIIWQMIKDKVCFTYL 391
            Q +P +SF  F        +  G  ++KV NPIVEMDGDEMTRIIW+ IKDK+ F ++
Sbjct:    1 QFSPNLSFSAFFPIITFTTATMGFQKIKVANPIVEMDGDEMTRIIWKYIKDKLIFPFV 58

[Close]

BLAST of CK937612 vs. ExPASy Swiss-Prot
Match: IDHC_TOBAC (Isocitrate dehydrogenase [NADP] OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 1.414e-26
Identity = 40/50 (80.00%), Postives = 47/50 (94.00%), Query Frame = 3

Query:  480 QLIFPYLDLDIKYYDLGILNRDATDDKVTVESAESTLKYNVAIKCATITP 629
            +LI P+L+LDIKY+DLG+ +RDATDDKVTVESAE+T KYNVAIKCATITP
Sbjct:   31 KLICPFLELDIKYFDLGLPHRDATDDKVTVESAEATQKYNVAIKCATITP 80

HSP 2 Score: 55.8398 bits (133), Expect = 1.414e-26
Identity = 23/34 (67.65%), Postives = 30/34 (88.24%), Query Frame = 2

Query:  290 DRVKVQNPIVEMDGDEMTRIIWQMIKDKVCFTYL 391
            D++KV+NPIVEMDGDEMTR+IW+ IKDK+   +L
Sbjct:    4 DKIKVENPIVEMDGDEMTRVIWKSIKDKLICPFL 37

[Close]

BLAST of CK937612 vs. ExPASy Swiss-Prot
Match: IDHC_SOYBN (Isocitrate dehydrogenase [NADP] OS=Glycine max GN=IDH1 PE=2 SV=2)

HSP 1 Score: 86.2705 bits (212), Expect = 4.060e-26
Identity = 40/50 (80.00%), Postives = 46/50 (92.00%), Query Frame = 3

Query:  480 QLIFPYLDLDIKYYDLGILNRDATDDKVTVESAESTLKYNVAIKCATITP 629
            +LI P+L+LDIKYYDLG+  RD TDDKVT+ESAE+TLKYNVAIKCATITP
Sbjct:   32 KLILPFLELDIKYYDLGLPYRDETDDKVTIESAEATLKYNVAIKCATITP 81

HSP 2 Score: 53.1434 bits (126), Expect = 4.060e-26
Identity = 22/33 (66.67%), Postives = 28/33 (84.85%), Query Frame = 2

Query:  293 RVKVQNPIVEMDGDEMTRIIWQMIKDKVCFTYL 391
            ++KV NPIVEMDGDEMTR+IW+ IKDK+   +L
Sbjct:    6 KIKVANPIVEMDGDEMTRVIWKSIKDKLILPFL 38

[Close]

BLAST of CK937612 vs. ExPASy Swiss-Prot
Match: IDHP_ASPNG (Isocitrate dehydrogenase [NADP], mitochondrial OS=Aspergillus niger GN=icdA PE=2 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.967e-25
Identity = 34/50 (68.00%), Postives = 42/50 (84.00%), Query Frame = 3

Query:  480 QLIFPYLDLDIKYYDLGILNRDATDDKVTVESAESTLKYNVAIKCATITP 629
            +LI PYLD+D+KYYDLG+  RD TDD+VTVE+AE+  KY V +KCATITP
Sbjct:  118 KLILPYLDVDLKYYDLGLEYRDQTDDQVTVEAAEAIKKYGVGVKCATITP 167

HSP 2 Score: 60.8474 bits (146), Expect = 1.967e-25
Identity = 40/94 (42.55%), Postives = 61/94 (64.89%), Query Frame = 2

Query:  110 AAATTKMMSSSTVAVKNPRWSLCSSNNSGLFSGHVNNRVSFRNQLAPAMSFRCFASATGIDRVKVQNPIVEMDGDEMTRIIWQMIKDKVCFTYL 391
            ++++T   SSS+V+ ++PR SL S++ S L S   + R +  + L    S      AT I ++KV+NP+VE+DGDEMTRIIWQ I++K+   YL
Sbjct:   36 SSSSTISSSSSSVSTRSPR-SLTSAS-SLLSSRTASARWTGLSSLNLTQSR---TMATEIPKIKVKNPVVELDGDEMTRIIWQEIREKLILPYL 124

[Close]

BLAST of CK937612 vs. ExPASy Swiss-Prot
Match: IDH1_CANTR (Isocitrate dehydrogenase [NADP], mitochondrial OS=Candida tropicalis GN=IDP1 PE=3 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 3.341e-25
Identity = 32/50 (64.00%), Postives = 43/50 (86.00%), Query Frame = 3

Query:  480 QLIFPYLDLDIKYYDLGILNRDATDDKVTVESAESTLKYNVAIKCATITP 629
            QLI PYLD+D+KYYDLGI +RDATDD++T+++A +  +Y V +KCATITP
Sbjct:   55 QLILPYLDVDLKYYDLGIESRDATDDQITIDAANAIKEYGVGVKCATITP 104

HSP 2 Score: 60.077 bits (144), Expect = 3.341e-25
Identity = 27/39 (69.23%), Postives = 33/39 (84.62%), Query Frame = 2

Query:  275 SATGIDRVKVQNPIVEMDGDEMTRIIWQMIKDKVCFTYL 391
            SAT  D++KV+NPIVE+DGDEMTRIIWQ IKD++   YL
Sbjct:   23 SATLADKIKVKNPIVELDGDEMTRIIWQKIKDQLILPYL 61

[Close]

BLAST of CK937612 vs. ExPASy Swiss-Prot
Match: IDH2_CANTR (Isocitrate dehydrogenase [NADP] peroxisomal OS=Candida tropicalis GN=IDP2 PE=1 SV=3)

HSP 1 Score: 74.7146 bits (182), Expect = 7.889e-24
Identity = 33/50 (66.00%), Postives = 42/50 (84.00%), Query Frame = 3

Query:  480 QLIFPYLDLDIKYYDLGILNRDATDDKVTVESAESTLKYNVAIKCATITP 629
            +LI PYL++D+KYYDLGI  RD TDDKVT ++AE+ L+Y V +KCATITP
Sbjct:   32 KLILPYLNVDLKYYDLGIEYRDKTDDKVTTDAAEAILQYGVGVKCATITP 81

HSP 2 Score: 56.9954 bits (136), Expect = 7.889e-24
Identity = 25/35 (71.43%), Postives = 29/35 (82.86%), Query Frame = 2

Query:  287 IDRVKVQNPIVEMDGDEMTRIIWQMIKDKVCFTYL 391
            I ++ V+NPIVEMDGDEMTRIIWQ IKDK+   YL
Sbjct:    4 IQKITVKNPIVEMDGDEMTRIIWQFIKDKLILPYL 38

[Close]

BLAST of CK937612 vs. ExPASy Swiss-Prot
Match: IDHP_MACFA (Isocitrate dehydrogenase [NADP], mitochondrial OS=Macaca fascicularis GN=IDH2 PE=2 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 1.170e-21
Identity = 31/50 (62.00%), Postives = 44/50 (88.00%), Query Frame = 3

Query:  480 QLIFPYLDLDIKYYDLGILNRDATDDKVTVESAESTLKYNVAIKCATITP 629
            +LI P++D+ +KY+DLG+ NRD TDD+VT++SA +T KY+VA+KCATITP
Sbjct:   69 KLILPHVDIQLKYFDLGLPNRDQTDDQVTIDSALATQKYSVAVKCATITP 118

HSP 2 Score: 50.8322 bits (120), Expect = 1.170e-21
Identity = 21/33 (63.64%), Postives = 27/33 (81.82%), Query Frame = 2

Query:  293 RVKVQNPIVEMDGDEMTRIIWQMIKDKVCFTYL 391
            R+KV  P+VEMDGDEMTRIIWQ IK+K+   ++
Sbjct:   43 RIKVAKPVVEMDGDEMTRIIWQFIKEKLILPHV 75

[Close]

BLAST of CK937612 vs. ExPASy Swiss-Prot
Match: IDHP_HUMAN (Isocitrate dehydrogenase [NADP], mitochondrial OS=Homo sapiens GN=IDH2 PE=1 SV=2)

HSP 1 Score: 73.559 bits (179), Expect = 1.170e-21
Identity = 31/50 (62.00%), Postives = 44/50 (88.00%), Query Frame = 3

Query:  480 QLIFPYLDLDIKYYDLGILNRDATDDKVTVESAESTLKYNVAIKCATITP 629
            +LI P++D+ +KY+DLG+ NRD TDD+VT++SA +T KY+VA+KCATITP
Sbjct:   69 KLILPHVDIQLKYFDLGLPNRDQTDDQVTIDSALATQKYSVAVKCATITP 118

HSP 2 Score: 50.8322 bits (120), Expect = 1.170e-21
Identity = 21/33 (63.64%), Postives = 27/33 (81.82%), Query Frame = 2

Query:  293 RVKVQNPIVEMDGDEMTRIIWQMIKDKVCFTYL 391
            R+KV  P+VEMDGDEMTRIIWQ IK+K+   ++
Sbjct:   43 RIKVAKPVVEMDGDEMTRIIWQFIKEKLILPHV 75

[Close]

BLAST of CK937612 vs. ExPASy Swiss-Prot
Match: IDHP_RAT (Isocitrate dehydrogenase [NADP], mitochondrial OS=Rattus norvegicus GN=Idh2 PE=1 SV=2)

HSP 1 Score: 71.2478 bits (173), Expect = 2.576e-21
Identity = 30/50 (60.00%), Postives = 44/50 (88.00%), Query Frame = 3

Query:  480 QLIFPYLDLDIKYYDLGILNRDATDDKVTVESAESTLKYNVAIKCATITP 629
            +LI P++D+ +KY+DLG+ NRD T+D+VT++SA +T KY+VA+KCATITP
Sbjct:   69 KLILPHVDVQLKYFDLGLPNRDQTNDQVTIDSALATQKYSVAVKCATITP 118

HSP 2 Score: 51.9878 bits (123), Expect = 2.576e-21
Identity = 21/33 (63.64%), Postives = 28/33 (84.85%), Query Frame = 2

Query:  293 RVKVQNPIVEMDGDEMTRIIWQMIKDKVCFTYL 391
            R+KV+ P+VEMDGDEMTRIIWQ IK+K+   ++
Sbjct:   43 RIKVEKPVVEMDGDEMTRIIWQFIKEKLILPHV 75

[Close]

BLAST of CK937612 vs. ExPASy Swiss-Prot
Match: IDHP_MOUSE (Isocitrate dehydrogenase [NADP], mitochondrial OS=Mus musculus GN=Idh2 PE=1 SV=3)

HSP 1 Score: 71.2478 bits (173), Expect = 2.576e-21
Identity = 30/50 (60.00%), Postives = 44/50 (88.00%), Query Frame = 3

Query:  480 QLIFPYLDLDIKYYDLGILNRDATDDKVTVESAESTLKYNVAIKCATITP 629
            +LI P++D+ +KY+DLG+ NRD T+D+VT++SA +T KY+VA+KCATITP
Sbjct:   69 KLILPHVDVQLKYFDLGLPNRDQTNDQVTIDSALATQKYSVAVKCATITP 118

HSP 2 Score: 51.9878 bits (123), Expect = 2.576e-21
Identity = 21/33 (63.64%), Postives = 28/33 (84.85%), Query Frame = 2

Query:  293 RVKVQNPIVEMDGDEMTRIIWQMIKDKVCFTYL 391
            R+KV+ P+VEMDGDEMTRIIWQ IK+K+   ++
Sbjct:   43 RIKVEKPVVEMDGDEMTRIIWQFIKEKLILPHV 75

The following BLAST results are available for this feature:

BLAST of CK937612 vs. ExPASy Swiss-Prot

BLAST of CK937612 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 30


Match Name	E-value	Identity	Description
IDHP_MEDSA	7.769e-28	78.00	Isocitrate dehydrogenase [NADP], chloroplastic (Fr...	[more]
IDHC_TOBAC	1.414e-26	80.00	Isocitrate dehydrogenase [NADP] OS=Nicotiana tabac...	[more]
IDHC_SOYBN	4.060e-26	80.00	Isocitrate dehydrogenase [NADP] OS=Glycine max GN=...	[more]
IDHP_ASPNG	1.967e-25	68.00	Isocitrate dehydrogenase [NADP], mitochondrial OS=...	[more]
IDH1_CANTR	3.341e-25	64.00	Isocitrate dehydrogenase [NADP], mitochondrial OS=...	[more]
IDH2_CANTR	7.889e-24	66.00	Isocitrate dehydrogenase [NADP] peroxisomal OS=Can...	[more]
IDHP_MACFA	1.170e-21	62.00	Isocitrate dehydrogenase [NADP], mitochondrial OS=...	[more]
IDHP_HUMAN	1.170e-21	62.00	Isocitrate dehydrogenase [NADP], mitochondrial OS=...	[more]
IDHP_RAT	2.576e-21	60.00	Isocitrate dehydrogenase [NADP], mitochondrial OS=...	[more]
IDHP_MOUSE	2.576e-21	60.00	Isocitrate dehydrogenase [NADP], mitochondrial OS=...	[more]

Pages

Properties


Property Name	Value
Genbank description	CGF1004778_D11 Developing fruit albedo at 80 DAFB in pTriplEx2 vector Citrus sinensis cDNA clone A80DAB0003_IIF_D11 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK937612 ID=CK937612; Name=CK937612; organism=Citrus sinensis; type=EST; length=766bp
GGGGAAGCAGAGTCACGGCGTATTAATTTAAAACGCCTCTTATCATTCCC
AAATGCTCAGGCTCATCAATAGTAGTGGTGGCAGTTTACGATCAAGCGCC
ATGTCACCTGCTGCTGCCACCACCAAAATGATGTCATCGTCAACCGTCGC
CGTTAAAAACCCCAGATGGTCACTCTGTTCTTCAAACAATTCCGGTTTAT
TCAGCGGACATGTGAACAATCGTGTGTCGTTTCGGAACCAGCTGGCTCCT
GCGATGTCGTTTCGGTGTTTTGCTAGTGCCACGGGGATTGATAGAGTCAA
GGTTCAGAATCCTATCGTTGAAATGGACGGCGATGAGATGACGAGGATTA
TTTGGCAGATGATAAAAGACAAAGTATGTTTTACTTATTTACCATAACTT
TCTTATTTTTCTTTTTCCAGAATAACCCTTTTAAAGCACATTGATAAAGT
TTAATGGGAATTAATTGCTTACATTGTTGCAGCTCATATTTCCTTACTTA
GATTTGGATATAAAGTATTATGACCTGGGGATATTGAATCGTGATGCAAC
TGATGACAAGGTTACCGTAGAAAGCGCTGAATCAACTCTAAAGTACAATG
TTGCCATCAAATGTGCCACAATAACTCCTGGTAAGCCATTATCCTTTTGC
AAGCTTTTTAAGCATTTTATTATTTTGGTACCAGAAATAAAAATTTCAGG
CAAGCCGAGTTTTATATAGTGTATGAAATTTTATNNNGCTCTTTACATTA
CATTGCAAGTGGATGG