CN186252

Overview
NameCN186252
Unique NameCN186252
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length860
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig1523 contig Csv1_Contig1523:347..1208. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CN186252 vs. ExPASy Swiss-Prot
Match: 1433D_SOYBN (14-3-3-like protein D OS=Glycine max GN=GF14D PE=2 SV=1)

HSP 1 Score: 274.248 bits (700), Expect = 6.364e-73
Identity = 139/172 (80.81%), Postives = 148/172 (86.05%), Query Frame = -2 
Query:  332 KRLNLNCRTFANDIMTVIDEHLILSASAGESTVFFYKMKGDYYRYLAEFKFGDEKKEAAANSMKXXXXXXXXXXADLPPTHPIRLGLALNFSVFYYEIMNSPERACHLAKQAFDEAISELDTLNEESYKDSTLIMQLLRDNLTLWTSDIPEDG-DAQKANGTAKVGGGEDAE 844
            +++ L      ND+M VIDEHLI SA+AGESTVF+YKMKGDYYRYLAEFK G+EKKEAA  SMK          ADLPPTHPIRLGLALNFSVFYYEI+NSPERACHLAKQAFDEAISELDTLNEESYKDSTLIMQLLRDNLTLWTSDIPEDG DAQK NGTAK+GGGEDAE
Sbjct:   90 QKVELELSNICNDVMRVIDEHLIPSAAAGESTVFYYKMKGDYYRYLAEFKSGNEKKEAADQSMKAYESATAAAEADLPPTHPIRLGLALNFSVFYYEILNSPERACHLAKQAFDEAISELDTLNEESYKDSTLIMQLLRDNLTLWTSDIPEDGEDAQKVNGTAKLGGGEDAE 261
[Close]
BLAST of CN186252 vs. ExPASy Swiss-Prot
Match: 1433B_VICFA (14-3-3-like protein B OS=Vicia faba PE=2 SV=1)

HSP 1 Score: 265.003 bits (676), Expect = 8.038e-73
Identity = 134/160 (83.75%), Postives = 142/160 (88.75%), Query Frame = -2 
Query:  332 DIMTVIDEHLILSASAGESTVFFYKMKGDYYRYLAEFKFGDEKKEAAANSMKXXXXXXXXXXADLPPTHPIRLGLALNFSVFYYEIMNSPERACHLAKQAFDEAISELDTLNEESYKDSTLIMQLLRDNLTLWTSDIPEDG-DAQKANGTAKVGGGEDAE 808
            D+M VIDEHLI SA+AGESTVF+YKMKGDYYRYLAEFK G+EKKEA   SMK          A+LPPTHPIRLGLALNFSVFYYEI+NSPERACHLAKQAFDEAISELDTLNEESYKDSTLIMQLLRDNLTLWTSDIPEDG D+QKANGTAK GGG+DAE
Sbjct:  102 DVMRVIDEHLIPSAAAGESTVFYYKMKGDYYRYLAEFKTGNEKKEAGDQSMKAYESATTAAEAELPPTHPIRLGLALNFSVFYYEILNSPERACHLAKQAFDEAISELDTLNEESYKDSTLIMQLLRDNLTLWTSDIPEDGEDSQKANGTAKFGGGDDAE 261

HSP 2 Score: 30.8018 bits (68), Expect = 8.038e-73
Identity = 13/16 (81.25%), Postives = 15/16 (93.75%), Query Frame = -1 
Query:  813 IKEYRQKVESELSDIC 860
            IKEYR KVE+ELS+IC
Sbjct:   85 IKEYRHKVETELSNIC 100
[Close]
BLAST of CN186252 vs. ExPASy Swiss-Prot
Match: 14339_SOLLC (14-3-3 protein 9 OS=Solanum lycopersicum GN=TFT9 PE=2 SV=2)

HSP 1 Score: 248.44 bits (633), Expect = 1.243e-67
Identity = 126/161 (78.26%), Postives = 137/161 (85.09%), Query Frame = -2 
Query:  332 NDIMTVIDEHLILSASAGESTVFFYKMKGDYYRYLAEFKFGDEKKEAAANSMKXXXXXXXXXXADLPPTHPIRLGLALNFSVFYYEIMNSPERACHLAKQAFDEAISELDTLNEESYKDSTLIMQLLRDNLTLWTSDIPEDG-DAQKANGTAKVGGGEDAE 811
            NDIM VID+HLI S SAGESTVF++KMKGDYYRYLAEFK G++KKE A  S+K          A+L PTHPIRLGLALNFSVFYYEIMNSPERACHLAKQAFDEAISELD+LNE+SYKDSTLIMQLLRDNLTLWTSD+PED  DAQK + T K GGGEDAE
Sbjct:  101 NDIMVVIDQHLIPSCSAGESTVFYHKMKGDYYRYLAEFKAGNDKKEVAELSLKAYQAATTAAEAELAPTHPIRLGLALNFSVFYYEIMNSPERACHLAKQAFDEAISELDSLNEDSYKDSTLIMQLLRDNLTLWTSDLPEDAEDAQKGDATNKAGGGEDAE 261

HSP 2 Score: 30.0314 bits (66), Expect = 1.243e-67
Identity = 13/16 (81.25%), Postives = 15/16 (93.75%), Query Frame = -1 
Query:  813 IKEYRQKVESELSDIC 860
            IKEY QKVESEL++IC
Sbjct:   85 IKEYLQKVESELTNIC 100
[Close]
BLAST of CN186252 vs. ExPASy Swiss-Prot
Match: 1433C_SOYBN (14-3-3-like protein C OS=Glycine max GN=GF14C PE=2 SV=1)

HSP 1 Score: 241.891 bits (616), Expect = 5.112e-66
Identity = 123/159 (77.36%), Postives = 138/159 (86.79%), Query Frame = -2 
Query:  335 NDIMTVIDEHLILSASAGESTVFFYKMKGDYYRYLAEFKFGDEKKEAAANSMKXXXXXXXXXXADLPPTHPIRLGLALNFSVFYYEIMNSPERACHLAKQAFDEAISELDTLNEESYKDSTLIMQLLRDNLTLWTSDIPEDGDAQKANGTAKVGGGEDA 811
            +DIMTVIDE+LI S+S+GE +VFFYKMKGDYYRYLAEFK GDE+KEAA +SMK          A+L  THPIRLGLALNFSVFYYEI+NSPERACHLAKQAFDEAISELDTL+EESYKDSTLIMQLLRDNLTLWTSDIPEDG+ QK + +A+  GG+DA
Sbjct:  101 SDIMTVIDEYLIPSSSSGEPSVFFYKMKGDYYRYLAEFKSGDERKEAADHSMKAYQLASTTAEAELASTHPIRLGLALNFSVFYYEILNSPERACHLAKQAFDEAISELDTLSEESYKDSTLIMQLLRDNLTLWTSDIPEDGEEQKVD-SARADGGDDA 258

HSP 2 Score: 31.187 bits (69), Expect = 5.112e-66
Identity = 14/16 (87.50%), Postives = 15/16 (93.75%), Query Frame = -1 
Query:  813 IKEYRQKVESELSDIC 860
            IKEYR KVESELS+IC
Sbjct:   85 IKEYRLKVESELSNIC 100
[Close]
BLAST of CN186252 vs. ExPASy Swiss-Prot
Match: 14339_ARATH (14-3-3-like protein GF14 mu OS=Arabidopsis thaliana GN=GRF9 PE=1 SV=2)

HSP 1 Score: 247.669 bits (631), Expect = 6.379e-65
Identity = 130/174 (74.71%), Postives = 142/174 (81.61%), Query Frame = -2 
Query:  332 KRLNLNCRTFANDIMTVIDEHLILSASAGESTVFFYKMKGDYYRYLAEFKFGDEKKEAAANSMKXXXXXXXXXXADLPPTHPIRLGLALNFSVFYYEIMNSPERACHLAKQAFDEAISELDTLNEESYKDSTLIMQLLRDNLTLWTSDIPEDG--DAQKANGTAKVG-GGEDAE 844
            +++ L       DIM+V+DEHLI SAS GESTVFF KMKGDYYRYLAEFK G+E+KEAA  S+K          A LPPTHPIRLGLALNFSVFYYEIMN+PERACHLAKQAFDEAISELDTLNEESYKDSTLIMQLLRDNLTLWTSDI E+G  DA K NG+AK G GG+DAE
Sbjct:   90 EKVELELSNICIDIMSVLDEHLIPSASEGESTVFFNKMKGDYYRYLAEFKSGNERKEAADQSLKAYEIATTAAEAKLPPTHPIRLGLALNFSVFYYEIMNAPERACHLAKQAFDEAISELDTLNEESYKDSTLIMQLLRDNLTLWTSDISEEGGDDAHKTNGSAKPGAGGDDAE 263
[Close]
BLAST of CN186252 vs. ExPASy Swiss-Prot
Match: 14338_SOLLC (14-3-3 protein 8 OS=Solanum lycopersicum GN=TFT8 PE=2 SV=2)

HSP 1 Score: 238.039 bits (606), Expect = 1.235e-64
Identity = 121/161 (75.16%), Postives = 134/161 (83.23%), Query Frame = -2 
Query:  332 NDIMTVIDEHLILSASAGESTVFFYKMKGDYYRYLAEFKFGDEKKEAAANSMKXXXXXXXXXXADLPPTHPIRLGLALNFSVFYYEIMNSPERACHLAKQAFDEAISELDTLNEESYKDSTLIMQLLRDNLTLWTSDIPEDG-DAQKANGTAKVGGGEDAE 811
            N+IMTVIDEHLI S +AGESTVF+YKMKGDYYRYLAEFK GD+KKE +  S+K          A+LP THPIRLGLALNFSVFYYEIMNSPERAC LAKQ FDEAISELD+LNE++YKD TLI+QLLRDNLTLWTSDIPEDG +A K +   KVG GEDAE
Sbjct:  101 NNIMTVIDEHLIPSCTAGESTVFYYKMKGDYYRYLAEFKTGDDKKEVSDLSLKAYQTATTTAEAELPITHPIRLGLALNFSVFYYEIMNSPERACQLAKQVFDEAISELDSLNEDNYKDGTLILQLLRDNLTLWTSDIPEDGEEAPKGDAANKVGAGEDAE 261

HSP 2 Score: 30.4166 bits (67), Expect = 1.235e-64
Identity = 13/16 (81.25%), Postives = 15/16 (93.75%), Query Frame = -1 
Query:  813 IKEYRQKVESELSDIC 860
            I+ YRQKVESEL+DIC
Sbjct:   85 IQGYRQKVESELTDIC 100
[Close]
BLAST of CN186252 vs. ExPASy Swiss-Prot
Match: 14312_ARATH (14-3-3-like protein GF14 iota OS=Arabidopsis thaliana GN=GRF12 PE=2 SV=1)

HSP 1 Score: 224.557 bits (571), Expect = 3.858e-60
Identity = 109/141 (77.30%), Postives = 122/141 (86.52%), Query Frame = -2 
Query:  386 DIMTVIDEHLILSASAGESTVFFYKMKGDYYRYLAEFKFGDEKKEAAANSMKXXXXXXXXXXADLPPTHPIRLGLALNFSVFYYEIMNSPERACHLAKQAFDEAISELDTLNEESYKDSTLIMQLLRDNLTLWTSDIPEDG 808
            DI+T+ID+HLI  A++GE+TVF+YKMKGDYYRYLAEFK   E+KEAA  S+K           +LP THPIRLGLALNFSVFYYEIMNSPERACHLAKQAFDEAI+ELDTL+EESYKDSTLIMQLLRDNLTLWTSD+PEDG
Sbjct:  105 DILTIIDQHLIPHATSGEATVFYYKMKGDYYRYLAEFKTEQERKEAAEQSLKGYEAATQAASTELPSTHPIRLGLALNFSVFYYEIMNSPERACHLAKQAFDEAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDLPEDG 245

HSP 2 Score: 28.8758 bits (63), Expect = 3.858e-60
Identity = 12/17 (70.59%), Postives = 15/17 (88.24%), Query Frame = -1 
Query:  810 IKEYRQKVESELSDICK 860
            IK YRQKVE EL++IC+
Sbjct:   88 IKGYRQKVEDELANICQ 104
[Close]
BLAST of CN186252 vs. ExPASy Swiss-Prot
Match: 14337_SOLLC (14-3-3 protein 7 OS=Solanum lycopersicum GN=TFT7 PE=2 SV=2)

HSP 1 Score: 224.557 bits (571), Expect = 1.455e-59
Identity = 108/148 (72.97%), Postives = 127/148 (85.81%), Query Frame = -2 
Query:  368 NDIMTVIDEHLILSASAGESTVFFYKMKGDYYRYLAEFKFGDEKKEAAANSMKXXXXXXXXXXADLPPTHPIRLGLALNFSVFYYEIMNSPERACHLAKQAFDEAISELDTLNEESYKDSTLIMQLLRDNLTLWTSDIPEDGDAQKAN 811
            +DI++VIDEHL+ S++ GESTVF+YKMKGDYYRYLAEFK GD++KEA+  S+K          +DL PTHPIRLGLALNFSVFYYEI+NSPERACHLAKQAFDEAI+ELD+L+EESYKDSTLIMQLLRDNLTLWTSD+ E G+  K +
Sbjct:   99 SDILSVIDEHLVPSSTTGESTVFYYKMKGDYYRYLAEFKAGDDRKEASEQSLKAYEAATATASSDLAPTHPIRLGLALNFSVFYYEILNSPERACHLAKQAFDEAIAELDSLSEESYKDSTLIMQLLRDNLTLWTSDLEEGGEHSKGD 246

HSP 2 Score: 26.9498 bits (58), Expect = 1.455e-59
Identity = 11/16 (68.75%), Postives = 13/16 (81.25%), Query Frame = -1 
Query:  813 IKEYRQKVESELSDIC 860
            IK YRQ+VE EL+ IC
Sbjct:   83 IKTYRQRVEDELTKIC 98
[Close]
BLAST of CN186252 vs. ExPASy Swiss-Prot
Match: 1433_TOBAC (14-3-3-like protein OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 218.779 bits (556), Expect = 3.173e-56
Identity = 106/145 (73.10%), Postives = 124/145 (85.52%), Query Frame = -2 
Query:  368 MTVIDEHLILSASAGESTVFFYKMKGDYYRYLAEFKFGDEKKEAAANSMKXXXXXXXXXXADLPPTHPIRLGLALNFSVFYYEIMNSPERACHLAKQAFDEAISELDTLNEESYKDSTLIMQLLRDNLTLWTSDIPEDGDAQKAN 802
            ++VIDEH++ S+++GESTVF+YKMKGDYYRYLAEFK GD++KEAA  S+K          ADL PTHPIRLGLALNFSVFYYEI+NSPERACHLAKQAFDEAI+ELD+L+EESYKDSTLIMQLLRDN TLWTSD+ E G+  K +
Sbjct:  101 LSVIDEHVVPSSTSGESTVFYYKMKGDYYRYLAEFKSGDDRKEAADQSLKAYEAATATASADLAPTHPIRLGLALNFSVFYYEILNSPERACHLAKQAFDEAIAELDSLSEESYKDSTLIMQLLRDNFTLWTSDLEEGGEHSKGD 245
[Close]
BLAST of CN186252 vs. ExPASy Swiss-Prot
Match: 14335_ORYSJ (14-3-3-like protein GF14-E OS=Oryza sativa subsp. japonica GN=GF14E PE=2 SV=1)

HSP 1 Score: 207.223 bits (526), Expect = 5.892e-55
Identity = 103/139 (74.10%), Postives = 118/139 (84.89%), Query Frame = -2 
Query:  389 IMTVIDEHLILSASAGESTVFFYKMKGDYYRYLAEFKFGDEKKEAAANSMKXXXXXXXXXXADLPPTHPIRLGLALNFSVFYYEIMNSPERACHLAKQAFDEAISELDTLNEESYKDSTLIMQLLRDNLTLWTSDIPED 805
            I+ ++D HL+ S++A ES VF+ KMKGDYYRYLAEFK G E+K+AA N+M           A+LPPTHPIRLGLALNFSVFYYEI+NSP+RAC+LAKQAFDEAISELDTL+EESYKDSTLIMQLLRDNLTLWTSDI ED
Sbjct:  107 ILKLLDSHLVPSSTAPESKVFYLKMKGDYYRYLAEFKTGAERKDAAENTMVAYKAAQDIALAELPPTHPIRLGLALNFSVFYYEILNSPDRACNLAKQAFDEAISELDTLSEESYKDSTLIMQLLRDNLTLWTSDISED 245

HSP 2 Score: 28.8758 bits (63), Expect = 5.892e-55
Identity = 12/16 (75.00%), Postives = 14/16 (87.50%), Query Frame = -1 
Query:  813 IKEYRQKVESELSDIC 860
            IKEYR K+E+ELS IC
Sbjct:   89 IKEYRGKIETELSKIC 104
The following BLAST results are available for this feature:
BLAST of CN186252 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 143
Match NameE-valueIdentityDescription
1433D_SOYBN6.364e-7380.8114-3-3-like protein D OS=Glycine max GN=GF14D PE=2... [more]
1433B_VICFA8.038e-7383.7514-3-3-like protein B OS=Vicia faba PE=2 SV=1[more]
14339_SOLLC1.243e-6778.2614-3-3 protein 9 OS=Solanum lycopersicum GN=TFT9 P... [more]
1433C_SOYBN5.112e-6677.3614-3-3-like protein C OS=Glycine max GN=GF14C PE=2... [more]
14339_ARATH6.379e-6574.7114-3-3-like protein GF14 mu OS=Arabidopsis thalian... [more]
14338_SOLLC1.235e-6475.1614-3-3 protein 8 OS=Solanum lycopersicum GN=TFT8 P... [more]
14312_ARATH3.858e-6077.3014-3-3-like protein GF14 iota OS=Arabidopsis thali... [more]
14337_SOLLC1.455e-5972.9714-3-3 protein 7 OS=Solanum lycopersicum GN=TFT7 P... [more]
1433_TOBAC3.173e-5673.1014-3-3-like protein OS=Nicotiana tabacum PE=2 SV=1[more]
14335_ORYSJ5.892e-5574.1014-3-3-like protein GF14-E OS=Oryza sativa subsp. ... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0002J19_f Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0002J19, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN186252 ID=CN186252; Name=CN186252; organism=Citrus sinensis; type=EST; length=860bp
GCCCCAAACATAGAGGATGTCCAAATAGCAGAAATCCTCCTGTGGCATTT
ACAGCTACATTAGAAAAACACCTTGTGCAATCAGACCAAATAATCCTCTA
CTACACTCCATCCATCATGACAGGAGGGAAACAATGGGAGGCAGGACAAG
TACGACCAATCATGTTTGGACAACCATGCTATAAAGTAAGCCATACCACA
ATATGGCACTATTAAAATGAAAATACAGCAAGCAAAAGACAATGGCACTT
TAAGCAAACACTAAGTTTGATTAACAAGTCTCATCCTTTCTCCCCCAATC
ACATAGCAACTCCTCGATGCTCTCCTTGTCACTCTGCATCTTCTCCCCCA
CCAACTTTGGCAGTGCCATTAGCCTTCTGGGCATCTCCATCTTCTGGAAT
GTCAGAAGTCCATAAGGTAAGGTTGTCCCTAAGAAGCTGCATAATTAAGG
TGCTGTCTTTGTAGGACTCCTCGTTGAGGGTGTCCAGCTCGGAAATAGCT
TCATCAAAAGCTTGTTTTGCAAGATGGCAAGCCCTTTCAGGAGAATTCAT
GATCTCATAATAAAAGACAGAGAAATTCAGAGCCAAACCCAATCGGATGG
GATGTGTAGGTGGCAAATCAGCCTCTGCAGCAGTGGTAGCTGTCTCATAC
GCTTTCATTGAATTAGCAGCAGCCTCTTTCTTCTCATCGCCAAACTTGAA
TTCAGCAAGGTACCGATAATAATCTCCTTTCATCTTATAGAAGAACACAG
TTGATTCACCAGCACTTGCTGAAAGAATAAGATGCTCATCAATGACAGTC
ATGATATCATTTGCAAATGTCCGACAATTCAGATTCAACCTTTTGCCTAT
ACTCCTTGAT
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