EY665376

Overview
NameEY665376
Unique NameEY665376
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length1228
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY665376 vs. ExPASy Swiss-Prot
Match: PRS8A_ARATH (26S protease regulatory subunit 8 homolog A OS=Arabidopsis thaliana GN=RPT6A PE=2 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 1.676e-27
Identity = 43/75 (57.33%), Postives = 50/75 (66.67%), Query Frame = 1 
Query:  304 VLHMILPSKLDPLVNLRKAGMVPDSTYDMVAALDARLDVIKEVNELPNLRPKLFESLERTQGAGVLKEGTQNKAK 528
            VLH++LPSK+DPLVNL K   VPDSTYDM+  LD ++  IKEV ELP   P+LFESL   Q  GVL  G     K
Sbjct:  134 VLHLVLPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGK 208

HSP 2 Score: 62.3882 bits (150), Expect = 1.676e-27
Identity = 31/45 (68.89%), Postives = 35/45 (77.78%), Query Frame = 3 
Query:  165 LLKLWEKIRFLYMVHPEGKYVVDIDHSIDITKIAPSTRVELRTDS 299
            ++K+  K + L  VHPEGKYVVDID SIDITKI PSTRV LR DS
Sbjct:   88 VVKVMGKNKVLVKVHPEGKYVVDIDKSIDITKITPSTRVALRNDS 132
[Close]
BLAST of EY665376 vs. ExPASy Swiss-Prot
Match: PRS8B_ARATH (26S protease regulatory subunit 8 homolog B OS=Arabidopsis thaliana GN=RPT6B PE=2 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 2.840e-27
Identity = 43/75 (57.33%), Postives = 50/75 (66.67%), Query Frame = 1 
Query:  304 VLHMILPSKLDPLVNLRKAGMVPDSTYDMVAALDARLDVIKEVNELPNLRPKLFESLERTQGAGVLKEGTQNKAK 528
            VLH++LPSK+DPLVNL K   VPDSTYDM+  LD ++  IKEV ELP   P+LFESL   Q  GVL  G     K
Sbjct:  134 VLHLVLPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGK 208

HSP 2 Score: 61.6178 bits (148), Expect = 2.840e-27
Identity = 31/36 (86.11%), Postives = 34/36 (94.44%), Query Frame = 2 
Query:   92 LYSRLRMLKE*LQLLQQPGSYVGEVVKVMGKNKVLV 199
            L SR+RML+E LQLLQ+PGSYVGEVVKVMGKNKVLV
Sbjct:   64 LNSRVRMLREELQLLQEPGSYVGEVVKVMGKNKVLV 99
[Close]
BLAST of EY665376 vs. ExPASy Swiss-Prot
Match: PRS8_DICDI (26S protease regulatory subunit 8 OS=Dictyostelium discoideum GN=psmC5 PE=1 SV=2)

HSP 1 Score: 77.411 bits (189), Expect = 2.878e-23
Identity = 41/74 (55.41%), Postives = 48/74 (64.86%), Query Frame = 1 
Query:  307 LHMILPSKLDPLVNLRKAGMVPDSTYDMVAALDARLDVIKEVNELPNLRPKLFESLERTQGAGVLKEGTQNKAK 528
            LH ILP+K+DPLV+L K   +PDSTYDMV  LD ++  IKEV ELP   P+LFESL   Q  GVL  G     K
Sbjct:  119 LHRILPNKIDPLVSLMKVEKIPDSTYDMVGGLDKQIKEIKEVIELPIKHPELFESLGIAQPKGVLLYGPPGTGK 192

HSP 2 Score: 53.1434 bits (126), Expect = 2.878e-23
Identity = 27/36 (75.00%), Postives = 31/36 (86.11%), Query Frame = 2 
Query:   92 LYSRLRMLKE*LQLLQQPGSYVGEVVKVMGKNKVLV 199
            L +R+RMLKE LQLL  PGS+V EVVK+MGKNKVLV
Sbjct:   48 LNNRVRMLKEELQLLTNPGSHVAEVVKLMGKNKVLV 83
[Close]
BLAST of EY665376 vs. ExPASy Swiss-Prot
Match: PRS8_NAEFO (26S protease regulatory subunit 8 homolog OS=Naegleria fowleri PE=2 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 3.074e-22
Identity = 39/74 (52.70%), Postives = 48/74 (64.86%), Query Frame = 1 
Query:  307 LHMILPSKLDPLVNLRKAGMVPDSTYDMVAALDARLDVIKEVNELPNLRPKLFESLERTQGAGVLKEGTQNKAK 528
            LH ILP+K+DPLV+L K   VPD+TY+MV  LD ++  IKEV ELP   P+LFESL   Q  GV+  G     K
Sbjct:  130 LHKILPNKIDPLVSLMKVEKVPDATYEMVGGLDEQIKEIKEVIELPIKHPELFESLGIAQPKGVIMYGPPGTGK 203

HSP 2 Score: 52.373 bits (124), Expect = 3.074e-22
Identity = 28/43 (65.12%), Postives = 33/43 (76.74%), Query Frame = 2 
Query:   92 LYSRLRMLKE*LQLLQQPGSYVGEVVKVMGKNKVLVYGSS*GE 220
            L  R+R LKE LQ LQ+PGS+VGEVVKVMGK KVL+  S  G+
Sbjct:   59 LNGRVRSLKEELQQLQEPGSHVGEVVKVMGKKKVLIKISQEGK 101
[Close]
BLAST of EY665376 vs. ExPASy Swiss-Prot
Match: PRS8_SCHPO (26S protease regulatory subunit 8 homolog OS=Schizosaccharomyces pombe GN=let1 PE=2 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 4.270e-21
Identity = 35/71 (49.30%), Postives = 45/71 (63.38%), Query Frame = 1 
Query:  316 ILPSKLDPLVNLRKAGMVPDSTYDMVAALDARLDVIKEVNELPNLRPKLFESLERTQGAGVLKEGTQNKAK 528
            ILP+K+DPLV+L     +PDSTY+MV  L+ ++  IKEV ELP   P+LFESL   Q  G+L  G     K
Sbjct:  122 ILPNKVDPLVSLMMVEKIPDSTYEMVGGLEKQIKEIKEVIELPVKHPELFESLGIPQPKGILLYGPPGTGK 192

HSP 2 Score: 56.6102 bits (135), Expect = 4.270e-21
Identity = 26/36 (72.22%), Postives = 33/36 (91.67%), Query Frame = 2 
Query:   92 LYSRLRMLKE*LQLLQQPGSYVGEVVKVMGKNKVLV 199
            L +R+R+L+E +QLLQ+PGSYVGEV+K MGKNKVLV
Sbjct:   48 LNARVRLLREEIQLLQEPGSYVGEVIKTMGKNKVLV 83
[Close]
BLAST of EY665376 vs. ExPASy Swiss-Prot
Match: PRS8_RAT (26S protease regulatory subunit 8 OS=Rattus norvegicus GN=Psmc5 PE=1 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 5.554e-21
Identity = 38/74 (51.35%), Postives = 47/74 (63.51%), Query Frame = 1 
Query:  307 LHMILPSKLDPLVNLRKAGMVPDSTYDMVAALDARLDVIKEVNELPNLRPKLFESLERTQGAGVLKEGTQNKAK 528
            LH ILP+K+DPLV+L     VPDSTY+M+  LD ++  IKEV ELP   P+LFE+L   Q  GVL  G     K
Sbjct:  123 LHKILPNKVDPLVSLMMVEKVPDSTYEMIGGLDKQIKEIKEVIELPVKHPELFEALGIAQPKGVLLYGPPGTGK 196

HSP 2 Score: 50.8322 bits (120), Expect = 5.554e-21
Identity = 22/45 (48.89%), Postives = 32/45 (71.11%), Query Frame = 3 
Query:  165 LLKLWEKIRFLYMVHPEGKYVVDIDHSIDITKIAPSTRVELRTDS 299
            +++  +K + L  VHPEGK+VVD+D +IDI  + P+ RV LR DS
Sbjct:   76 VVRAMDKKKVLVKVHPEGKFVVDVDKNIDINDVTPNCRVALRNDS 120
[Close]
BLAST of EY665376 vs. ExPASy Swiss-Prot
Match: PRS8_PIG (26S protease regulatory subunit 8 OS=Sus scrofa GN=PSMC5 PE=2 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 5.554e-21
Identity = 38/74 (51.35%), Postives = 47/74 (63.51%), Query Frame = 1 
Query:  307 LHMILPSKLDPLVNLRKAGMVPDSTYDMVAALDARLDVIKEVNELPNLRPKLFESLERTQGAGVLKEGTQNKAK 528
            LH ILP+K+DPLV+L     VPDSTY+M+  LD ++  IKEV ELP   P+LFE+L   Q  GVL  G     K
Sbjct:  123 LHKILPNKVDPLVSLMMVEKVPDSTYEMIGGLDKQIKEIKEVIELPVKHPELFEALGIAQPKGVLLYGPPGTGK 196

HSP 2 Score: 50.8322 bits (120), Expect = 5.554e-21
Identity = 22/45 (48.89%), Postives = 32/45 (71.11%), Query Frame = 3 
Query:  165 LLKLWEKIRFLYMVHPEGKYVVDIDHSIDITKIAPSTRVELRTDS 299
            +++  +K + L  VHPEGK+VVD+D +IDI  + P+ RV LR DS
Sbjct:   76 VVRAMDKKKVLVKVHPEGKFVVDVDKNIDINDVTPNCRVALRNDS 120
[Close]
BLAST of EY665376 vs. ExPASy Swiss-Prot
Match: PRS8_MOUSE (26S protease regulatory subunit 8 OS=Mus musculus GN=Psmc5 PE=1 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 5.554e-21
Identity = 38/74 (51.35%), Postives = 47/74 (63.51%), Query Frame = 1 
Query:  307 LHMILPSKLDPLVNLRKAGMVPDSTYDMVAALDARLDVIKEVNELPNLRPKLFESLERTQGAGVLKEGTQNKAK 528
            LH ILP+K+DPLV+L     VPDSTY+M+  LD ++  IKEV ELP   P+LFE+L   Q  GVL  G     K
Sbjct:  123 LHKILPNKVDPLVSLMMVEKVPDSTYEMIGGLDKQIKEIKEVIELPVKHPELFEALGIAQPKGVLLYGPPGTGK 196

HSP 2 Score: 50.8322 bits (120), Expect = 5.554e-21
Identity = 22/45 (48.89%), Postives = 32/45 (71.11%), Query Frame = 3 
Query:  165 LLKLWEKIRFLYMVHPEGKYVVDIDHSIDITKIAPSTRVELRTDS 299
            +++  +K + L  VHPEGK+VVD+D +IDI  + P+ RV LR DS
Sbjct:   76 VVRAMDKKKVLVKVHPEGKFVVDVDKNIDINDVTPNCRVALRNDS 120
[Close]
BLAST of EY665376 vs. ExPASy Swiss-Prot
Match: PRS8_HUMAN (26S protease regulatory subunit 8 OS=Homo sapiens GN=PSMC5 PE=1 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 5.554e-21
Identity = 38/74 (51.35%), Postives = 47/74 (63.51%), Query Frame = 1 
Query:  307 LHMILPSKLDPLVNLRKAGMVPDSTYDMVAALDARLDVIKEVNELPNLRPKLFESLERTQGAGVLKEGTQNKAK 528
            LH ILP+K+DPLV+L     VPDSTY+M+  LD ++  IKEV ELP   P+LFE+L   Q  GVL  G     K
Sbjct:  123 LHKILPNKVDPLVSLMMVEKVPDSTYEMIGGLDKQIKEIKEVIELPVKHPELFEALGIAQPKGVLLYGPPGTGK 196

HSP 2 Score: 50.8322 bits (120), Expect = 5.554e-21
Identity = 22/45 (48.89%), Postives = 32/45 (71.11%), Query Frame = 3 
Query:  165 LLKLWEKIRFLYMVHPEGKYVVDIDHSIDITKIAPSTRVELRTDS 299
            +++  +K + L  VHPEGK+VVD+D +IDI  + P+ RV LR DS
Sbjct:   76 VVRAMDKKKVLVKVHPEGKFVVDVDKNIDINDVTPNCRVALRNDS 120
[Close]
BLAST of EY665376 vs. ExPASy Swiss-Prot
Match: PRS8_BOVIN (26S protease regulatory subunit 8 OS=Bos taurus GN=PSMC5 PE=2 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 5.554e-21
Identity = 38/74 (51.35%), Postives = 47/74 (63.51%), Query Frame = 1 
Query:  307 LHMILPSKLDPLVNLRKAGMVPDSTYDMVAALDARLDVIKEVNELPNLRPKLFESLERTQGAGVLKEGTQNKAK 528
            LH ILP+K+DPLV+L     VPDSTY+M+  LD ++  IKEV ELP   P+LFE+L   Q  GVL  G     K
Sbjct:  123 LHKILPNKVDPLVSLMMVEKVPDSTYEMIGGLDKQIKEIKEVIELPVKHPELFEALGIAQPKGVLLYGPPGTGK 196

HSP 2 Score: 50.8322 bits (120), Expect = 5.554e-21
Identity = 22/45 (48.89%), Postives = 32/45 (71.11%), Query Frame = 3 
Query:  165 LLKLWEKIRFLYMVHPEGKYVVDIDHSIDITKIAPSTRVELRTDS 299
            +++  +K + L  VHPEGK+VVD+D +IDI  + P+ RV LR DS
Sbjct:   76 VVRAMDKKKVLVKVHPEGKFVVDVDKNIDINDVTPNCRVALRNDS 120
The following BLAST results are available for this feature:
BLAST of EY665376 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 15
Match NameE-valueIdentityDescription
PRS8A_ARATH1.676e-2757.3326S protease regulatory subunit 8 homolog A OS=Ara... [more]
PRS8B_ARATH2.840e-2757.3326S protease regulatory subunit 8 homolog B OS=Ara... [more]
PRS8_DICDI2.878e-2355.4126S protease regulatory subunit 8 OS=Dictyostelium... [more]
PRS8_NAEFO3.074e-2252.7026S protease regulatory subunit 8 homolog OS=Naegl... [more]
PRS8_SCHPO4.270e-2149.3026S protease regulatory subunit 8 homolog OS=Schiz... [more]
PRS8_RAT5.554e-2151.3526S protease regulatory subunit 8 OS=Rattus norveg... [more]
PRS8_PIG5.554e-2151.3526S protease regulatory subunit 8 OS=Sus scrofa GN... [more]
PRS8_MOUSE5.554e-2151.3526S protease regulatory subunit 8 OS=Mus musculus ... [more]
PRS8_HUMAN5.554e-2151.3526S protease regulatory subunit 8 OS=Homo sapiens ... [more]
PRS8_BOVIN5.554e-2151.3526S protease regulatory subunit 8 OS=Bos taurus GN... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-101-007-B10-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY665376 ID=EY665376; Name=EY665376; organism=Citrus sinensis; type=EST; length=1228bp
ATATTTTATTTTGCTGCGTCGGGTCGGGATTCCGGGTCGACCCCGCGTCC
GATGAACACCATCTCTGTCGCCTCGAATCCCACCCTTTTAACTCTATTCT
AGACTGCGGATGCTTAAAGAATAATTACAGCTTCTTCAACAGCCTGGATC
CTATGTCGGTGAGGTTGTTAAAGTTATGGGAAAAAATAAGGTTCTTGTAT
ATGGTTCATCCTGAGGGGAAGTATGTTGTTGATATTGATCACAGTATTGA
TATAACCAAGATCGCTCCGTCAACAAGAGTTGAACTTCGTACTGACAGCT
AGTGTTCTTCATATGATCCTGCCAAGCAAATTAGATCCCCTGGTCAACCT
TAGGAAGGCTGGAATGGTCCCTGATTCTACTTATGACATGGTTGCTGCCT
TGGATGCCCGACTTGACGTGATCAAGGAGGTCAATGAGCTTCCTAATCTA
CGTCCCAAATTGTTCGAGTCTCTAGAAAGAACTCAAGGCGCCGGTGTATT
GAAGGAAGGAACACAGAATAAGGCAAAACATTGGTGAGAAGAGTTAAGGT
CCACCAAATGAATTGACTATTCACAAAGTTTCGGGTTCGAGATAGTCCAG
AATATCTTGGCTAAAGGACCGGAAGGCTAAAAAATTTTGGTATAGGATGG
TGAATGTCCCTTCATAATTTTAGGGAGAATTGCAAGTACTATATGGATCA
ATGAATCTAGCCCATATTGATTTTATAAATACCACAATTTCCTTATCTTC
CACCACTAAAGTATTAAGTCCTATAATATTGGTATTTGATCTATCAATTA
TATTCAACTCCCTACTATGGGATGTAAACTCAACATGTAATATCATACAA
GAGTAAACTCCAAGCAATAGAAACGTAAGAAGGTTATCGCTTTAATCTTA
AATATTAACAGTAAAAGTGAAGAACGATATACCAAATGCCATCCGTTCTT
AACACTGATATTACATAGATATATGCAGTGTAAAAAATACACAACAATGT
AAAATAACTTCTACATCTATTTGATACCAGCTCTTCTCATGACTTGCATT
CTTCCTAAATCTCTTTACTAATCATCACTAAATTAAAATGATTGTTATAT
ATGTAGCTCTAATGGTTACCCTAAACTATAATACACATAAATATAAAAGA
AGTTACTGCGATTACCAAGGCAATGATCAGCGAATTCAAGACGATGTCTA
ACTGTTGCAACTAGAATAAGAGAAGTAT
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